65 results about "Aspergillus wentii" patented technology

The invention discloses a feed compound enzyme-containing dedicated enzyme for growing pigs and a preparation method of the feed compound enzyme-containing dedicated enzyme, and belongs to the technical field of preparation of enzyme preparations. The dedicated enzyme for the growing pigs is prepared by scientifically compounding aspergillus niger cultures, acidic xylanase, phytase, beta-dextranase, cellulase, amylase, acid proteinase, Chinese herb extracts, a protective agent and an activating agent, wherein the aspergillus niger cells in the aspergillus niger cultures can inhibit the growth of pathogenic escherichia coli, inhibit the growth and breeding of aspergillus flavus, disintegrate the aspergillus flavus, promote the growth of animals, adjust gastrointestinal tract flora to be balanced, and enhance whole immunity during the processes of growth and fermentation, in addition, the fermentation liquid crude enzyme liquid of the aspergillus niger cultures contains different enzyme activity such as protease activity, mannase activity, alpha-galactase activity and pectinase activity, and the dedicated enzyme is specially suitable for being added in the feed for the growing pigs.

The invention especially discloses a method for preparing soluble corn peptide from Aspergillus niger fermented maize yellow powder, which belongs to the technical field of food biochemistry. According to the invention, Aspergillus niger is used in the method, main components of a medium comprise maize yellow powder, wheat bran and water, a proper amount of cane sugar and dipotassium hydrogen phosphate are added into the main components, and the steps of centrifugation, pumping filtration, decolouring, ultrafiltration and gel chromatography are carried out to obtain a soluble corn peptide solution. The method has the advantages of a simple process, low cost and a few by-products and enables the obtained corn peptide to have high water-solubility, good security, etc.

The invention relates to a novel method for preparing fructo-oligosaccharide, which adopts a thallus anaerobic fermentation technology to produce the fructo-oligosaccharide with the component contentover 50 percent. The method has simple process, does not need a sterile air preparation system equipped for producing the fructo-oligosaccharide by anaerobic fermentation and relevant electric power appliance, and has less equipment investment and low energy resource consumption, thereby greatly saving energy sources. The novel method is applicable to producing the fructo-oligosaccharide of Aspergillus niger BLB-11, which comprises the following steps: carrying out primary or secondary cultivation to obtain a strain for enlarging the culture; transferring the strain to an anaerobic fermentation tank; under the anaerobic condition, adjusting sucrose concentration to 10-60 percent, adding 1-40 percent of bacteroid cells, setting the pH value to be 4-7, carry out the anaerobic fermentation for 10-36 hours at the reaction temperature of 25-60 DEG C to obtain a reaction solution with the content of the fructo-oligosaccharide over 50 percent, micro-filtering, decolorizing, refining and condensation the reaction solution to obtain a fructo-oligosaccharide liquid product, drying the fructo-oligosaccharide liquid product to to obtain a solid fructo-oligosaccharide product after concentration.

The invention discloses an aspergillus oryzae and an application method thereof in preparing sauce fragrant flavor material. The aspergillus oryzae was preserved in a China Typical Culture Collection Center in July 1st, 2012, and the preservation number is CCTCC NO.M2012265. The aspergillus oryzae provided by the invention and aspergillus niger are mixedly fermented, a fermentation condition is controlled, enzyme systems produced by the two aspergilli are utilized for the controllable enzymolysis of raw material to obtain enzymolysis liquid containing rich small molecular weight peptide (1-5kDa), amino acid and sauce fragrant material, and finally the sauce fragrant meat flavor material is obtained. Compared with the meat flavor material prepared in a method of directly adding commercial enzymic preparations, the meat flavor material prepared in the method disclosed by the invention has the advantages of low cost, mellow taste, strong sauce fragrance, nature, safety and the like.

The invention relates to an Aspergillus niger lipase and its preparing process, which consists of separating and screening wild strains S-8341 of Aspergillus niger in nature, obtaining fine production strains S-7749 through industrial microorganism mutagenesis breeding, subjecting the strains to enlargement culture and low temperature high oxygen fermentation to obtain aspergillus niger lipase whose N-terminal amino acid sequence is ATADAAAFPDLHRAAKLSSA.

A de-bonding enzyme produced by Aspergillus niger HYA4 and its use in flax de-gumming are disclosed. The process is carried out by taking potato glucose agar culturing medium as tapered-land preservative culturing medium, taking solid fermenting culture medium as bran, bean dregs, ammonia sulfate and ammonium nitrate, putting ferment under room temperature, leaching by natural water stilly, filtering to obtain coarse enzyme liquid, diluting, and determining pectase and xylanase activity. It's cheap and non-toxic; has more enzyme yield, complete varieties, shorter production period, various raw material sources, more yielding rate and combing rate and less water-source pollution.

The invention discloses a new method for extracting resveratrol by fermenting a peanut root by an Aspergillus niger, which belongs to the technical field of extracting natural products by utilizing green plants and is characterized in that the resveratrol is extracted from the peanut root by enzymolysis of a cellulase generated by the peanut root fermentation. The method has the advantages that enzymatic extraction is carried out by utilizing the cellulase generated by the peanut root fermentation by the Aspergillus niger to replace a commercial cellulase, with short extraction time and mild extraction condition, thus greatly improving the obtaining rate of the resveratrol, largely reducing the manufacturing cost of the resveratrol, and being suitable for large-scale industrial production.

The organic selenium derived from aspergillus oryzae and a preparation method thereof of the present invention belong to the field of food and health product additives. The organic selenium of the present invention uses Aspergillus oryzae as a carrier, and exists in the form of Aspergillus oryzae cells, aqueous solution or dry powder. Its preparation method uses selenium compound as raw material, through the process of preparing selenium-containing medium, inoculating and culturing Aspergillus oryzae, collecting Aspergillus oryzae, washing to remove extracellular selenium, sterilizing or / and drying; the organic aspergillus oryzae cell form is obtained by drying. Selenium, or the organic selenium in the form of an aqueous solution obtained from thalline autolysis, or the organic selenium in the form of dry powder obtained by drying the aqueous solution. Aspergillus oryzae has the characteristics of fast growth and high content of organic selenium in selenium-enriched Aspergillus oryzae cells. During the fermentation process, Aspergillus oryzae can convert inorganic selenium into organic selenium, which is easily metabolized and absorbed by the human body. Using Aspergillus oryzae containing organic selenium instead of common Aspergillus oryzae as an additive in food and health products can supplement selenium in a small amount and continuously, and effectively prevent and treat diseases caused by selenium deficiency.

The invention relates to a method for preparing compost by washing wool junk microbial fermentation. Microorganism accounting for 10 percent of the total mass of silt is implanted in the washing wool silt and fermented for 15-30 days at the temperature of 45-65 DEG C. The microorganisms are the composite of bacteria, fungi and actinomycetes, and the number ratio of the bacteria, the fungi and the actinomycetes is 10-30:0.5-2.5:0-1.5. The bacteria are bacillus subtilis, Pseudomonas aeruginosa and Bacillus mucilaginosus with the number ratio of 1-5:0.5-3:0.3-1.5; and the fungi are Aspergillus niger and Penicillium simplicissimum with the number ratio of 1-3:0.5-1.5. The compost produced by fermentation contains 30-80% of organic matter, 3-15% of total nitrogen, 2-10% of total phosphorus and 1-7% of total potassium.

The invention discloses a preparation method of beta-glucosaccharase. The method includes the following steps: selecting Aspergillus niger as parent strain through screening, inoculating the Aspergillus niger, the inoculum size of which is 106-108cfu / g, to bran, rice bran and ammonia chloride, wherein in a solid culture medium prepared from the bran, the rice bran and the ammonia chloride according to the weight percentage of 90-100 percent: 0-10 percent:1-3 percent, the initial water content of the culture medium is 65-75 percent, and the pH is 5.5-6.5, culturing for 48-80h at the temperature of 25-30 DEG C and detecting the activity of beta-glucosaccharase to reach 300-430U / g. The method adopts solid fermentation, uses cheap and rich subsidiary agricultural products as fermentation substrate, simultaneously optimizes the culture medium and culture conditions and achieves high yield of the beta-glucosaccharase under solid fermentation conditions, thus providing scientific basis to the industrialization development of the beta-glucosaccharase.

The invention discloses a preparation process of broad bean paste fermented by mixed aspergillus oryzae and lactobacillus plantarum. The broad bean paste is prepared from the raw materials by weight as follows: 40-80 parts of broad beans, 12-20 parts of edible salt, 15-25 parts of flour, 0.01-0.02 parts of aspergillus oryzae powder, 0.002-0.008 parts of lactobacillus plantarum powder and 40-80 parts of water. The mixed aspergillus oryzae and lactobacillus plantarum is adopted for fermentation, the viscosity of the broad bean paste is improved, so that quality of each batch of the broad bean paste keeps uniform, the risk of pollution of a product by aflatoxin is reduced, and the safety edibility is significantly increased; meanwhile, a temperature control and humidity control technology is adopted, during koji making, the temperature is controlled at 28-32 DEG C, the humidity is controlled to be 75%-80%, the reproduction rate of dominant fermentation strains, namely, the aspergillus oryzae and the lactobacillus plantarum, is increased, koji making time is shortened to 24 hours from traditional 3-5 days, and production cost is saved.

The invention discloses a controlled culture method of Aspergillus niger for producing citric acid and aims to overcome defects that the workload is heavy, the operation is inconvenient, the method is not applicable to mass production and the like during usage of a wheat bran culture medium in the prior art. Mouldy bran taking corncob grans as the culture medium component is used, and the culture mode is innovated, so that the elimination rate is decreased, the cost is reduced, and the working efficiency is improved; meanwhile, the operation is convenient, and above all, the capacity is improved.

The invention discloses an aspergillus niger gene knockout method and belongs to the technical field of biotechnology. A shearing element and a gene knockout expression cassette are integrated in an aspergillus niger genome simultaneously, resistance genes are induced to be eliminated by inducible promoters later, and accordingly, resistance can be eliminated by transforming aspergillus niger once. The method can be used for knocking out multiple genes continuously; an effective method and a new concept are provided for metabolic transformation of aspergillus niger.

The invention relates the aspergillus Niger variant, and spore is ochre. The bacterial comprises the following biology characters: the bacterial is on the culture medium to grow for 7 days at 25Deg.C, the diameter is 45-70mm; the texture is villiform and granular; conidiospore is light brown; the reverse surface of bacterial colony is yellow. Using the aspergillus Niger variant, and adopting solid invoice method, the alpha-galactosidase is made. The enzymatic activity is 286IU / g, and the maximum is 345IU / g.

The invention relates to aspergillus oryzae ZA210. The aspergillus oryzae has the advantages of thick and solid hypha, high germination rate, low raw material consumption rate, and high comprehensiveenzyme activity, so that the fermented raw oil has the advantages of high total nitrogen index, high glutamic acid and alanine content, low arginine content and the like. The aspergillus oryzae is applied to soy sauce production so as to ferment the raw oil with pure flavor, gentle and harmonized taste, outstanding freshness and sweetness, abundant taste, so that the zero-addition pure brewing soysauce can be industrially produced easily, and energy conservation and efficiency increment are realized.

The invention discloses a method for producing calcium gluconate by fermenting Aspergillus niger, which comprises seed culture and fermentation culture, and is characterized in that: Aspergillus niger TN9518 inclined-plane stains are adopted to ferment liquid glucose, the glucose is converted into gluconic acid which exchanges calcium with a calcium-containing compound to generate calcium gluconate, and the calcium gluconate is orderly subjected to aseptic filtration, concentration and crystallization, separation and drying to form a finished product of the calcium gluconate. Through the method, the damage to partial glucose structures due to 121 DEG C sterilization of the glucose is avoided; byproducts do not exist, and the oxidation is concentrated; inorganic salt in a culture medium is omitted; the purity of the product is improved; film condensation is adopted, so steam for normal condensation is saved and the production process is simplified; the labor capacity is improved; secondary water consumption and wastewater generated in the production process are reduced; and recycle is performed synchronously so the environmental pollution is lightened; therefore, the method has the advantages of low energy consumption and low cost.

The present invention relates to a co-culture method of Sphingomonas sp. bacterial strain and Aspergillus sp. fungus strain, in which the novel Sphingomonas sp. bacterial strain KMK-001 is cultured in a liquid medium and the novel Aspergillus sp. strain KMC-901 separately cultured in another liquid medium is added to the above culture solution, a novel glionitrin biosynthesized therefrom and a pharmaceutical composition comprising the said glionitrin or its pharmaceutically acceptable salt as an active ingredient. The glionitrin herein has strong cytotoxic effect on cancer cells and has antibiotic effect on 10 pathogenic bacteria including the novel Sphingomonas sp. bacterial strain KMK-001, so that it can be effectively applied in antibiotics or anti-cancer agents.

The invention relates to an Aspergillus niger genetic engineering strain with high yield of organic acid under a low-dissolved oxygen condition. The construction steps of the genetic engineering strain are as follows: step 1, constructing a heterologously-expressed vhb gene plasmid, wherein the sequence fragment of the gene vhb is controlled by an Aspergillus niger 3-glycerophosphate dehydrogenasegene promoter PgpdA; and step 2, acquiring a heterogeneously-expressed vhb gene strain so as to obtain the Aspergillus niger genetic engineering strain with high yield of organic acid under a low-dissolved oxygen condition. Based on the natural characteristics that Aspergillus niger generates organic acid, the Aspergillus niger genetic engineering strain is obtained by modifying the physiologicalcharacteristics of Aspergillus niger through genetic recombination. Experiments prove that the ability of the aspergillus niger genetic engineering strain for producing organic acid under the condition of low dissolved oxygen is obviously improved, and an excellent strain is provided for preparing organic acid by a microbial fermentation method.

The invention relates to a large-scale culture method of lactobacillus acidophilus. The method is characterized by comprising the steps as follows: 1) preparation of a strain: the strain consists of aspergillus niger spores and lactobacillus acidophilus in the weight ratio being (8-9):1; 2) preparation of a culture medium: the culture medium consists of rice bran, soybean meal, NaCl and wheat bran, and all substance components are mixed and stirred uniformly; 3) solid fermentation: the culture medium is poured in a solid fermentation tank, the solid fermentation tank is inoculated with the aspergillus niger spores, the relative humidity and the air supply are kept unchanged, and culture is performed for 48 h. The method realizes industrial solid fermentation and low-cost and large-scale culture and has the benefits of low cost and high production efficiency.

The present invention discloses a method for quickly constructing an aspergillus niger recombinant strain and belongs to the technical field of genetic engineering. Aspergillus niger albA gene and aspergillus niger kuSA gene are simultaneously sheared through CRISPR-Cas 9, and an aspergillus niger kuSA mutant strain is rapidly screened out through spore phenotyping. Compared with a homologous recombination method for mutating the kuSA gene, the method obviously improves homozygote probability of the homologous recombination strain, and enables damage probability of the white mutant kuSA to reach 50% and probability of homozygote to reach 100%.

The invention provides a culture method of Aspergillus niger seeds. The transplantation opportunity is selected by monitoring the acid production speed, the glucose consumption speed or the glucoamylase activity in the culture process. The invention specifically discloses a method for determining the acid production speed, the glucose consumption speed or the glucoamylase activity and discloses influence of the concentration of dissolved oxygen on the culture process. The defect that the growth state of the seeds is judged mainly by observing the sizes and shapes of Aspergillus niger balls in a conventional culture method is overcome. The standard deviation of fermentation results of the seeds cultured with the method is smaller, the seed fermentation in adjacent batches is more stable, the fermentation conversion rate is increased, and the fermentation period is remarkably shortened.

Relating to the field of biological engineering, the invention provides to a method for Aspergillus oryzae to secrete lignin to prepare peroxidase. The production process includes: subjecting straw to crushing, pre-wetting and other pre-treatment technologies, and subjecting Aspergillus oryzae to shaking culture, first class seed culture, and mixing with straw to undergo solid state fermentation so as to obtain a lignin peroxidase-containing straw fermentation product with enzyme activity of 3-800U / g dry fermentation product, and carrying out water extraction, molecular interception and freeze drying on the fermentation product so as to obtain a crude enzyme preparation with enzyme activity of 900-8000U / g dry crude product. The fermentation process is simple, has no pollution to the environment, and causes no resource waste. The lignin peroxidase prepared by the method can be used to degrade lignin macromolecular structures, release small molecule substances, and improve the digestion utilization rate of lignin-containing straw, thus being helpful to improve the feeding value of straw.

The invention discloses a mechanized production process for multi-strain yellow wine raw wheat koji and belongs to the technical field of brewing. The production process comprises the following steps: respectively processing the koji starters of Aspergillus niger CCTCC NO:M2015202 and Aspergillus oryzae CCTCC NO:M2015201, and simultaneously manufacturing the bacterium solution of Lactobacillus plantarum CCTCC NO:M2015118 and Lactobacillus hilgardii CGMCC No:8098; and crushing the wheat, and respectively inoculating the Aspergillus niger, the Aspergillus oryzae and the monascus koji starters in a disk starter making machine to obtain the yellow wine raw wheat koji with good characteristic. The mechanized production process has the characteristics of short fermenting time, high enzyme activity, easy culture and labor cost conservation.

The invention discloses a method for producing chymosin by aspergillus niger solid fermentation. The method adopts a solid basal medium and comprises the following steps of adding a nitrogen source and an acidic nutrient solution containing one or more inorganic salts and an amino acid into the solid basal medium to wet it, mixing well, carrying out a sterilization process at a temperature of 121 DEG C for 30 minutes, cooling, inoculating the sterilized solid basal medium with an aspergillus niger strain, carrying out solid fermentation at a fermentation temperature of 35 to 37 DEG C for 72 to 100 hours, carrying out solid-liquid extraction through adopting water as a solvent, carrying out centrifugation and extraction filtration, and carrying out the processes of vacuum rotation, evaporation, concentration and freeze-drying on supernate obtained by the previous step to obtain crude dry powder of chymosin. Chymosin which has the advantages of low added amount in raw milk such as milk and the like, rapid curd speed, specific catalytic site and low capacity for non-specific protein hydrolysis is produced through the method.

The invention discloses a method for producing potassium gluconate through fermentation of Aspergillus niger, which comprises the following steps: performing one-step jet on starch and starch-containing materials, and performing double-enzyme glucose preparation; using an Aspergillus niger P1205X21 strain which is resistant to high-concentration K<+> to ferment the glucose solution, and feeding an ion membrane KOH solution in the fermentation process to obtain a potassium gluconate solution; and by using a thermal crystallization process of multiple-effect continuous evaporation and crystallization, directly evaporating and crystallizing in a multiple-effect evaporation crystallizer to obtain the potassium gluconate finished product. The invention has the advantages that the raw materials are rich and accessible, the cost is low, the fermentation product is simplex, the product quality is high and easy to control, no waste water and waste gas are generated, no calcium gluconate mother liquor and calcium sulfate waste are generated, the environmental pollution is greatly reduced, the process route is short, evaporation, crystallization, drying and other procedures during calcium gluconate preparation are reduced, vapor is saved, the power consumption is reduced, and the production cost is greatly lowered.

The invention relates to the technical field of pharmaceutical chemistry and microbial medicines, and in particular relates to a norditerpenoid compound obtained from a fermentation product of Aspergillus wentii, a separation and purification method for the norditerpenoid compound and application of the norditerpenoid compound in the aspect of inhibiting the activity of aquatic disease bacteria. The norditerpenoid compound comprises a compound 1 and a compound 2 as shown in the formula I; the compound 1 and the compound 2 are a pair of isomers; the molecular formula of each of the compound 1 and the compound 2 is C19H26O3; the bacteriostatic activity tests show that the compound 1 and the compound 2 have outstanding inhibitory activity on the aquatic disease bacteria such as Edvardsiella tarda, Vibrio harveyi and Vibrio parahaemolyticus, and the minimum inhibitory concentration (MIC) of each of the compound 1 and the compound 2 is 8.0 microgram / mL, and the norditerpenoid compound is expected to be developed into a novel aquatic disease bacteria prevention and treatment drug. The formula I is shown in the description.

The invnetion provides aspergillus fumigatus strain TMS-26 for producing taxol. The strain can produce the taxol under the condition of liquid fermentation. The strain is collected in China Center For Type Culture Collection (Wuhan University) on June 15, 2014, and the collection number is CCTCC M2014258. The taxol yield of the strain under the conditions of PDA liquid culture medium, 28 DEG C, 60r / min and dark culture for 10 to 12d can reach 696.24[mu]g / L to the maximum degree. The novel strain provided by the invention provides good study basis for the breeding of good strains in industrial large-scale production, and the application prospects are wide.

The invention belongs to the technical field of microorganisms, and particularly relates to a production method for organic acid fermentation aspergillus niger mycelium extract. The method includes the steps that an organic acid fermentation aspergillus niger mycelium obtained after starch raw materials are adopted for producing organic acid through aspergillus niger fermentation is filtered, washed and subjected to serous mixing, and mycelium serous fluid is obtained; a cell wall-breaking enzyme is added into the mycelium serous fluid for carrying out a cell wall-breaking reaction, and wall-broken serous fluid is obtained; protease and nuclease are added into the serous fluid for carrying out a hydrolysis reaction, so that hydrolysate is obtained; the hydrolysate is subjected to centrifugal separation, so that a liquid phase is obtained; the liquid phase is directly subjected to evaporation and concentration, so that the aspergillus niger mycelium extract is prepared. According to the method, pollution is avoided, the reaction condition is moderate, product quality is good, and high-valued utilization of the aspergillus niger mycelium is realized.

The invention discloses a method for improving yield of resveratrol through fermentation of aspergillus niger. An aspergillus niger strain is cultured in a triangular flask culture medium for 48h, 2% by mass of hypha is evenly added to giant knotweed rhizomes with humidity of 2:1, and the stacking fermentation is carried out at a constant temperature of 40 DEG C for 30h. Through a solid stacking fermentation method, polydatin in giant knotweed rhizomes is converted into resveratrol, and the solid fermentation condition is optimized. In the method, a strong acid-base reaction is not generated, and glycoside does not need to be crudely extracted; meanwhile, expensive equipment does not need to be added, and the method is easy to popularize. The extraction yield of resveratrol is increased to 1.197% and is increased by 30% compared with that of the existing method, so the very obvious technical effects are achieved.

The invention relates to the field of insecticides, particularly application of a natural seaweed endophytic fungus diterpene alkaloid compound. The seaweed endophytic fungus diterpene alkaloid compound has a disinsection function, and is disclosed as Formula (I). In the invention, the fungus Aspergillus oryzae cf-2 which is separated from ocean red algae Heterosiphonia is fermentated, extracted and separated to obtain the diterpene alkaloid natural compound. The insecticidal activity experiment indicates that the lethality rate of the 100 mcg / ml diterpene alkaloid compound for Artemia is 60.3%.