Aspergillus oryzae ZA210 and application thereof
A technology of ZA210 and Aspergillus oryzae, which is applied in the direction of microorganisms, fungi, biochemical equipment and methods, etc., can solve the problems of heavy post-bitterness, acidity and saltiness of fermented crude oil, etc. silk thick effect
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Embodiment 1
[0045] The screening of embodiment 1-Aspergillus oryzae ZA210 obtains
[0046] 1. Strain mutagenesis
[0047] Mature slant spores of As3.951 strain of Zhongke were taken to make spore suspension, and the spore suspension was subjected to ARTP mutagenesis. Dilute the mutagenized spore suspension to 10 with 0.85% sterile saline -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Concentration, made spore dilution.
[0048] Take 0.2 mL of the spore dilution and spread it on the casein plate medium, and incubate at 31° C. in the dark for 96 hours. During the cultivation process, observe and record the hyphal growth and sporulation of the colony, the speed of the formation of the transparent circle, and the size of the transparent circle. Compared with the starting strain As3.951, the colony with the characteristics of thick hyphae, large transparent circle and slightly less sporulation was selected.
[0049] The target colony selected on the above casein plate was further spread on the...
Embodiment 2
[0076] The effect verification of embodiment 2-Aspergillus oryzae ZA210 bacterial strain
[0077] The new strain ZA210 screened by mutagenesis and the starting strain As3.951 were used in the production of soy sauce simultaneously. After the koji making was finished, the enzyme activity was sampled and tested, and the crude oil index was tested after the fermentation was finished, and the sensory evaluation of the crude oil was carried out.
[0078] The test results of various indicators are shown in Table 6, Table 7, Table 8, and Table 9, and the sensory evaluation results are shown in image 3 .
[0079] Table 6 Koji quality analysis
[0080]
[0081] As shown in the results in Table 6, the number of spores of the Aspergillus oryzae ZA210 strain was slightly less than that of As3.951, the mycelium was thick and vigorous, and the activity of protease, leucine aminopeptidase E, acid carboxypeptidase A and glutamine The enzyme activity was higher than the corresponding ind...
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