Application of bacillus subtilis amino-peptidase in preparing flavor functional nutrient rice
A technology of Bacillus subtilis and preparation process, applied in the fields of enzyme preparations and food additives, can solve problems such as poor taste, and achieve the effects of improving antioxidant capacity, increasing umami taste, and improving application space
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[0041] Example 1
[0042] The fermentation broth of Bacillus subtilis Zj016 ([Food and Fermentation Industry] 2006, Vol. 32, Issue 3 has been published) was centrifuged at 4℃, 10000rpm for 10min to collect 3.5L fermentation broth, and added (NH) at 4℃ 4 ) 2 SO 4 700 g (stirring while adding), centrifugation at 4°C 10000rpm for 10min to collect 3.6L of supernatant, use 50kDa ultrafiltration membrane to concentrate to 1.4L, flow rate 35mL / min; select 30kDa ultrafiltration membrane to desalinate 1.4L, flow rate 40 mL / min; freeze-dry the concentrated desalting solution to obtain solid aminopeptidase, the enzyme activity of the obtained solid aminopeptidase is 7.9×10 3 U / g. Use 5g rice powder as the substrate, 50mL water as the medium, and use neutral protease and solid aminopeptidase for enzymatic hydrolysis. The mass ratio of neutral protease (E1): solid aminopeptidase (E2) is 5:1 , Wherein the total amount of compound enzyme added is 0.8% of the substrate; 50℃ enzymatic hydrolys...
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[0044] Example 2
[0045] The fermentation broth of Bacillus subtilis Zj016 was centrifuged at 4℃, 10000rpm for 10min to collect 2.5L of fermentation broth, and added (NH 4 ) 2 SO 4 500 g (stirring while adding), centrifugation at 4°C, 10000rpm for 10min to collect 2.5L of clear liquid, select 50kDa ultrafiltration membrane to concentrate to 0.85L, flow rate 30mL / min; select 30kDa ultrafiltration membrane to desalinate 0.85L, flow rate 40 mL / min, freeze-dry the concentrated desalting solution to obtain solid aminopeptidase. The enzyme activity of the obtained solid aminopeptidase is 6.7×10 3 U / g. Use 5g rice powder as the substrate, 50mL water as the medium, and use neutral protease and solid aminopeptidase for enzymatic hydrolysis. The mass ratio of neutral protease (E1): solid aminopeptidase (E2) is 5:1 , The total amount of compound enzyme added is 1.2% of the substrate; 50℃ enzymatic hydrolysis 6h. Boil the enzyme for 5 minutes, add 95% ethanol to a system ethanol volume...
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