Application of bacillus subtilis amino-peptidase in preparing flavor functional nutrient rice
A technology of Bacillus subtilis and preparation process, applied in the fields of enzyme preparations and food additives, can solve problems such as poor taste, and achieve the effects of improving antioxidant capacity, increasing umami taste, and improving application space
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Embodiment 1
[0042] Centrifuge the fermentation broth of Bacillus subtilis Zj016 ([Food and Fermentation Industry] 2006 Volume 32, Issue 3) at 4°C and centrifuge at 10,000rpm for 10min to collect 3.5L of fermentation broth, add (NH 4 ) 2 SO 4 700 g (stirring while adding), centrifuge at 10000rpm at 4°C for 10min to collect 3.6L of clarified liquid, select 50kDa ultrafiltration membrane to concentrate to 1.4L, flow rate 35mL / min; select 30kDa ultrafiltration membrane to desalinate 1.4L, flow rate 40 mL / min; freeze-dry the concentrated desalted solution to obtain solid aminopeptidase, and the enzyme activity of the obtained solid aminopeptidase is 7.9×10 3 U / g. Using 5g of rice powder as the substrate and 50mL of water as the medium, use a mixture of neutral protease and solid aminopeptidase for enzymolysis. The mass ratio of neutral protease (E1) : solid aminopeptidase (E2) is 5:1 , wherein the total amount of compound enzyme added is 0.8% of the substrate; enzymatic hydrolysis at 50°...
Embodiment 2
[0045] Centrifuge the Bacillus subtilis Zj016 fermentation broth at 4°C for 10 min at 10,000 rpm to collect 2.5L of the fermentation broth, add (NH 4 ) 2 SO 4 500 g (stirring while adding), 4°C, 10000rpm centrifuge for 10min to collect 2.5L of clarified liquid, select 50kDa ultrafiltration membrane to concentrate to 0.85L, flow rate 30mL / min; select 30kDa ultrafiltration membrane to desalt 0.85L, flow rate 40 mL / min, freeze-dry the concentrated desalted solution to obtain solid aminopeptidase. The enzymatic activity of the obtained solid-state aminopeptidase is 6.7×10 3 U / g. Using 5g of rice powder as the substrate and 50mL of water as the medium, use a mixture of neutral protease and solid aminopeptidase for enzymolysis. The mass ratio of neutral protease (E1) : solid aminopeptidase (E2) is 5:1 , wherein the total amount of compound enzyme added is 1.2% of the substrate; 50 ° C enzyme hydrolysis for 6 hours. Boil the enzyme for 5 minutes, add ethanol with a volume fra...
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