43 results about "Peroxyl radical" patented technology

The invention discloses a casein-sourced multifunctional peptide, and belongs to the field of food science and nutriology. The casein is subjected to enzymolysis through pepsin, a method combining activity verification and mass spectrum identification is adopted, the novel multifunctional peptide YPE with a clear sequence is screened and verified, the highest ABTS clearance rate of the casein peptide YPE can reach 77.76%, the highest hydroxyl radical clearance rate of the casein peptide YPE can reach 40.18%, and the casein peptide YPE can be used for preparing the novel multifunctional peptide YPE. The inhibition rate of alpha-glucosidase reaches 30.47%, the inhibition rate of escherichia coli is 10.18%, and the limitation of an existing single-function peptide in application is overcome.

The invention belongs to the technical field of biological fermentation, and particularly relates to a preparation method and application of a high-activity antioxidant product based on hericium erinaceus-hawthorn solid state fermentation. Activating the strain to prepare a seed solution; adding hawthorn into the rice solid culture medium, inoculating hericium erinaceus, performing constant-temperature culture, drying a fermentation product to constant weight at normal temperature and normal pressure after the culture is finished, crushing, and sieving for later use. The antioxidant effect of the fermentation product is comprehensively evaluated and verified through a systematic in-vitro chemical antioxidant experiment (ABTS < + >, DPPH free radical and hydroxyl free radical scavenging) and an in-vivo zebra fish oxidative stress model, and solid data support is provided for application of the fermentation product in healthy products. The resource high-value utilization way of the hawthorn and the hericium erinaceus is widened, and a technical scheme and a product prototype are provided for developing novel food raw materials, health-care products or medicine auxiliary materials with definite antioxidant functions.

The invention provides an agropyron cristatum-Chinese date antioxidant composition as well as a preparation method and application thereof, and belongs to the technical field of functional foods. The composition consists of wheatgrass and Chinese dates. The composition provided by the invention can be used for removing hydroxyl radicals and exerting an anti-oxidation effect. The hydroxyl radical scavenging rate of the composition is obviously higher than that of single wheatgrass or single jujube, and the composition shows a synergistic anti-oxidation effect.

The invention belongs to the technical field of biology, and particularly relates to acetylated raffinose and application thereof. According to the invention, acetylation modification is carried out on raffinose by using an acetylation reagent to obtain acetylated raffinose, the acetylated raffinose can significantly improve the scavenging ability and total antioxidant ability of raffinose on DPPH free radicals, and the scavenging ability on hydroxyl free radicals and the reduction ability on iron ions are also improved to a certain extent. The acetylated raffinose provided by the invention has potential application value in development of functional foods and antioxidants.

The invention discloses a method for extracting nanocrystallized bamboo fiber cell walls from bamboo green, which comprises the following steps: thermally activating a bamboo green material from natural bamboo wood in a weak base solution, and then carrying out organic acid mediated in-situ peroxy radical oxidation by using an organic acid-hydrogen peroxide mixture aqueous solution, then the water suspension is subjected to hydraulic separation under the hydraulic action to obtain bamboo fiber cells, and finally the obtained bamboo fiber cells are subjected to mechanical crushing and nanocrystallization refining treatment to obtain the needed nanocrystallization bamboo fiber cell walls. According to the method for extracting the nanocrystallized bamboo fiber cell wall, the natural moso bamboo is directly used as the raw material, the bamboo does not need to be pulped or smashed in advance, the preparation process is simple, the method is suitable for large-scale production, and the obtained nanocrystallized bamboo fiber cell wall product can be widely applied to the fields of food thickening, low-GI food additives and the like.

The invention discloses a fermentation medium for high yield of oudemansiella oosporium mycelium polysaccharide and a culture method thereof. The fermentation medium comprises the following components in percentage by mass: 20-25% of potatoes, 1-4% of corn flour, 0.2-0.4% of peptone, 0.2-0.4% of CaSO4, 0.05-0.15% of MgSO4 and the balance of water, and the pH value of the fermentation medium is 5.5-6.5. According to the culture medium, growth of Oudemansiella ovalis hyphae can be accelerated by regulating and controlling the proportion of different nutritional components, synthesis of Oudemansiella ovalis polysaccharide can be promoted, the final sugar yield of the strain is increased by 7 times compared with that of conventional culture by adopting the optimized fermentation culture medium and fermentation process, and the sugar yield can reach 167.37 mg / g. The scavenging rates of DPPH free radicals, ABTS free radicals and hydroxyl free radicals of the oospore oudemansiella sp. Polysaccharide are 95.02%, 98.08% and 93.74% respectively, and the scavenging rates are obviously higher than those of previous research results. The invention provides a new direction for developing natural antioxidants, and also provides theoretical significance and application value for developing oudemansiella oosporium mycelium fermentation products.

This invention discloses the application of a porcine-derived Bacillus in antioxidant activity, wherein the porcine-derived Bacillus is used to prepare antioxidant products. The porcine-derived Bacillus is Bacillus subtilis YB-X-721, classified as Bacillus subtilis, and was deposited at the China General Microbiological Culture Collection Center on November 10, 2023, with accession number CGMCC No. 28942. The fermentation supernatant, bacterial suspension, and cell-free extract of the porcine-derived Bacillus subtilis YB-X-721 of this invention all possess certain DPPH free radical, superoxide anion free radical, and hydroxyl free radical scavenging abilities, as well as a certain reducing ability, and can be used to prepare antioxidant products. Furthermore, when the porcine-derived Bacillus subtilis YB-X-721 of this invention is added to piglet feed as an animal feed additive, it can improve the growth performance of weaned piglets, significantly increasing average daily weight gain, and significantly reducing average daily feed intake, feed conversion ratio, and diarrhea rate.

The invention relates to a method for extracting roselle anthocyanin through enzymolysis-ultrasonic assistance, a product and application of the product. The method comprises the following steps: degreasing roselle powder, and performing enzymolysis for 24 hours by using cellulase and pectinase (20U / g respectively) under the conditions that the pH is 5.0 and the temperature is 40 DEG C; an acetone-hydrochloric acid mixed solution (the volume fraction of hydrochloric acid is 36%) with the liquid-material ratio being 10: 1 serves as an extracting agent, and ultrasonic extraction is conducted for 34 minutes at the temperature of 58 DEG C and the power of 160 W; and finally, centrifuging, extracting with ethyl acetate, and purifying to obtain an anthocyanin product. According to the method, the extraction rate reaches 3.087 mg / g, the main component of the product is cyanidin-3-glucoside, the antioxidant and antibacterial activity is remarkable, the scavenging rates of DPPH, ABTS and hydroxyl radicals are 86.26%, 81.34% and 93.75% respectively under the concentration of 3.0 mg / mL, and the scavenging rates are superior to those of vitamin C with the same concentration; under the concentration of 11mg / mL, the compound has an obvious inhibition effect on escherichia coli and staphylococcus aureus. The process is efficient and mild, the product activity is excellent, and the method is suitable for development and application of natural antioxidants and antibacterial agents.

This invention discloses a method for extracting polysaccharides from *Microcystis aeruginosa* and its applications. The method includes fat removal, alcohol-soluble molecule removal, ultrasonic water extraction, flavonoid removal, protein removal, pigment removal, ethanol precipitation, small molecule substance removal, and freeze-drying. This invention combines directional stepwise impurity removal with ultrasonic water extraction to achieve efficient separation of polysaccharides from impurities. The optimal ultrasonic extraction process was determined using response surface methodology: a fixed ultrasonic extraction power of 100W, an ultrasonic extraction temperature of 49℃, an extraction time of 61 min, a solid-liquid ratio of 1:20.5 g / mL, and three extractions. Under these conditions, the polysaccharide yield reached 1.45%. The obtained *Microcystis aeruginosa* polysaccharides exhibit significant antioxidant activity, showing good scavenging effects against DPPH free radicals, hydroxyl free radicals, and ABTS free radicals, with the total reducing power increasing in a dose-dependent manner. This invention provides a simple and controllable extraction process, yielding polysaccharides with high purity and good activity, which can be used as natural antioxidants in functional foods, health products, and cosmetics.

The invention provides functional saccharomyces cerevisiae and application thereof in fermentation of pueraria polysaccharide, and belongs to the technical field of microbial fermentation. The invention discloses a saccharomyces cerevisiae Y25, which has the physiological characteristics of high ethanol yield, low higher alcohol yield, high ester yield and good tolerance to ethanol, acid and high temperature, can improve the quality of white spirit and enrich and coordinate the flavor of the white spirit, and can be used for fermenting kudzuvine root polysaccharide, so that the obtained fermented polysaccharide is high in yield, strong in oxidation resistance and high in yield. The polysaccharide content is increased by 9.79% compared with commercial Angel yeast, and the DPPH free radical scavenging rate and the hydroxyl free radical scavenging rate are respectively increased by 10.32% and 18.78% compared with the commercial Angel yeast. The saccharomyces cerevisiae Y25 disclosed by the invention has far-reaching social and economic significance on enhanced utilization of functional brewing microbial resources and innovation and upgrading of a traditional brewing process.

The invention discloses a multifunctional active peptide from mackerel viscera and application of the multifunctional active peptide. The mackerel viscera extract is prepared by adopting a single exogenous enzyme hydrolysis method, the extract has small molecule peptides with ACE inhibitory activity, three ACE inhibitory peptides are obtained by further identification and screening, the amino acid sequences are respectively GPPVPGP, GPMGPMGP and SPGPVGMP, and the half inhibitory concentration IC50 values of the ACE inhibitory peptides are respectively 80.84 + / -1.12 mu g / mL, 177.41 + / -3.26 mu g / mL and 163.25 + / -3.46 mu g / mL. Wherein the heptapeptide GPPVPGP with the optimal activity also has the function of removing DPPH free radicals and hydroxyl free radicals, and can be used for adjuvant therapy for reducing hypertension or applied to other fields.

The application discloses a kind of alpha-lactalbumin multifunctional peptides with antioxidant, anticoagulant and tyrosinase inhibitory activity and application thereof, and belongs to the field of food, biopharmaceutical and cosmetic technology.The multifunctional peptide SEKLDQWLCEKL is screened, and the DPPH clearance rate and ABTS clearance rate of the multifunctional peptide SEKLDQWLCEKL are highest, which can reach 62.32% and 88.32% respectively, the clearance rate of hydroxyl radical is highest, which can reach 78.89%, PT and TT are respectively extended by 3.4 s and 4.7 s, and the inhibition rate of tyrosinase is highest, which can reach 89.07%, which overcomes the limitation of existing single functional peptide in application.

The invention relates to the technical field of active polysaccharide preparation, in particular to a hemerocallis fulva polysaccharide DLP-N as well as a preparation method and application of the hemerocallis fulva polysaccharide DLP-N. Grinding the dried day lily into powder, degreasing, desugaring and air-drying; preparing a deep eutectic solvent; using an extraction deep eutectic solvent; performing alcohol precipitation; performing freeze-drying; removing protein by a Sevage method; depigmentation is performed; carrying out DEAE-52 cellulose anion exchange chromatography; and carrying out sephadex SephacrylS-400 chromatography to obtain the white flocculent refined polysaccharide. The day lily polysaccharide component with remarkable antioxidant and cancer-inhibiting double activities is prepared by taking day lily as a raw material, the yield of the day lily polysaccharide component is as high as 25.6%, high-value utilization of resources is effectively realized, and the day lily polysaccharide component has excellent scavenging performance on DPPH free radicals, ABTS free radicals, hydroxyl free radicals and superoxide anion free radicals. Meanwhile, cancer cell proliferation can be effectively inhibited, and a definite structure-function relationship and functional gradient are shown.

This invention provides a peroxide free radical source generation and calibration system, relating to the field of pollutant monitoring technology. The system includes a free radical generation module and an online calibration module. The free radical generation module includes a main photolysis cavity, which generates ·H and ·OH by photolysis of H2O using ultraviolet light. The ·H generated in the main photolysis cavity reacts with O2 to generate HO2 free radicals. The online calibration module includes a calibration channel connected to the main photolysis cavity and a separate background channel. NO standard gas is introduced into the calibration channel for calibration; NO standard gas is added to the background channel as background. This invention can accurately acquire NO2 signals under both active and passive scenarios, effectively deducting background interference and achieving quantitative measurement of HO2.

The invention relates to an anti-oxidation Chinese grass turtle egg protein peptide extract as well as a preparation method and application thereof, and belongs to the technical field of biological preparation. The invention relates to a Chinese grass turtle egg protein peptide extract, which comprises peptide fragments with amino acid sequences as shown in SEQ ID NO.1-6. The preparation method comprises the steps of separation, enzymolysis, purification and the like, and specifically comprises the following steps: separating out egg white, freeze-drying, preparing egg white crude protein peptide hydrolysate by using an ultrasonic-assisted enzymolysis method, and finally purifying, freezing and drying to obtain protein peptide powder. The Chinese grass turtle egg protein peptide extract is prepared by adopting an ultrasonic-assisted papain method, the obtained polypeptide is relatively high in purity and clear in main component and structure, the activity is well maintained, and the Chinese grass turtle egg protein peptide extract has the capabilities of removing ABTS free radicals, DPPH free radicals and hydroxyl free radicals and enhancing iron ion reduction, and has a remarkable antioxidant effect.

The invention belongs to the technical field of biological medicine, and relates to sargassum polysaccharide as well as a preparation method and application thereof. The uniform acidic polysaccharide WZSP-2 is separated and purified from the Weizhou Sargassum through hot water extraction, ethanol precipitation, protein and color removal, anion exchange column and gel column chromatography and other methods, and the physicochemical properties, monosaccharide composition and structural morphology of the Weizhou Sargassum are systematically characterized. In-vitro experiments show that the WZSP-2 has remarkable DPPH, ABTS and hydroxyl radical scavenging capacity and total reducing power, can effectively inhibit the activity of alpha-amylase and alpha-glucosidase, can be combined with glycocholate and taurocholate, and has a bacteriostatic effect on methicillin-resistant staphylococcus aureus (MRSA) at the same time. The sargassum polysaccharide provided by the invention integrates antioxidant activity, hypoglycemic activity, hypolipidemic activity and antibacterial activity, provides a new source for developing multifunctional natural products, and has a good application prospect in the fields of functional food, health care products and medical auxiliary materials.

The present application relates to the technical field of plant extract, and discloses a preparation method of Hericium erinaceus extract and application of the Hericium erinaceus extract in cosmetics, which comprises the following steps: taking Hericium erinaceus raw materials, adopting ethanol reflux extraction, combining the extraction liquid, and obtaining extract by vacuum concentration; liquid-liquid extraction is carried out by using ethyl acetate, and the Hericium erinaceus extract is obtained by concentration and freeze-drying. The main active ingredient of Hericium erinaceus extract is Hericenones C by HPLC separation. The activity verification experiment shows that the extract has significant antioxidant activity, the IC50 value of DPPH free radical scavenging capacity is 239±7 μg / mL; the IC50 value of hydroxyl radical (·OH) scavenging capacity is 547±15 μg / mL; the Trolox equivalent antioxidant capacity (TEAC) of ABTS+· free radical scavenging capacity is 477.3±3.1 μmol TE / g; the extract has significant proliferation promoting effect on HaCaT cells, and the maximum proliferation rate is 146.44±1.27%. The present application solves the technical problems that the components of Hericium erinaceus extract are not clear and the UVB resistance activity is not developed, and provides a technical basis for the application of the extract in cosmetics as anti-wrinkle, repair and anti-aging raw materials.

This invention provides a fermenting *Lactobacillus mucinus* strain that can accelerate alcohol metabolism and improve hangover symptoms, along with its applications. This strain exhibits high safety, lacking resistance genes and pathogenic virulence genes, and demonstrates strong tolerance to bile salts, artificial gastric juice, and artificial intestinal juice. The bacterium possesses strong antioxidant capabilities, exhibiting potent scavenging abilities against ABTS, DPPH, and hydroxyl radicals. This fermenting *Lactobacillus mucinus* can increase the alcohol dehydrogenase content in a mouse model of alcoholic liver disease, demonstrating significant application value in antioxidation, alcohol detoxification, and hangover prevention. Furthermore, this bacterium can produce melatonin and convert NR to niacin.

The invention discloses yellow yellow wine and a preparation method thereof, and belongs to the technical field of wine brewing. The method comprises the following steps: by taking broomcorn millet as a raw material, soaking, cooking congee, mixing with yeast, carrying out main fermentation, carrying out secondary fermentation, adding vacuum freeze-dried yellow pollen after the secondary fermentation is completed, and then carrying out after-ripening, squeezing, decocting wine, aging and storing and finishing. According to the method, active ingredients in the day lily are fully dissolved out and stably retained by optimizing the day lily adding time and the after-ripening process. In the obtained yellow yellow wine, the total phenol content is not lower than 0.50 mg / mL, the flavonoid content is not lower than 0.12 mg / mL, the vitamin C content is not lower than 11 mu g / mL, the hydroxyl radical scavenging rate is not lower than 85%, the amino acid content is not lower than 3300 mu g / mL, and the yellow yellow wine has remarkable antioxidant activity and enhanced nutrition flavor.

The invention provides lactobacillus reuteri IMAU12801 with probiotic characteristics and application of the lactobacillus reuteri IMAU12801, and belongs to the technical field of food microorganisms. The invention provides lactobacillus reuteri IMAU12801 with probiotic characteristics, and the preservation number of the lactobacillus reuteri IMAU12801 is CGMCC (China General Microbiological Culture Collection Center) No.31226. The lactobacillus reuteri IMAU12801 is a lactobacillus reuteri strain with probiotic characteristics, and the preservation number of the lactobacillus reuteri IMAU12801 is CGMCC No.31226. The lactobacillus reuteri IMAU12801 provided by the invention has good tolerance to gastrointestinal fluid, and has various probiotic characteristics, such as bacteriostatic potential, DPPH (1, 1-diphenyl-2-picrylhydrazyl) and hydroxyl radical scavenging capacity, antioxidant activity and the like. After the lactobacillus reuteri IMAU12801 is compounded with a basic fermentation agent to ferment cow milk, the stability of the fermented milk under the storage condition of 4 DEG C can be improved. The invention provides a brand new thought for the utilization and development of the lactobacillus reuteri IMAU12801.

The invention discloses a bovine serum albumin-sourced multifunctional peptide capable of resisting oxidation, reducing blood sugar and inhibiting tyrosinase as well as preparation and application thereof, and belongs to the technical fields of food, biological pharmacy and cosmetics. A novel multifunctional peptide SFLYEYSRR with a clear sequence is screened and verified, the highest clearance rate of the peptide SFLYEYSRR to ABTS can reach 89.93%, the highest clearance rate of the peptide SFLYEYSRR to DPPH free radicals can reach 35.48%, the highest clearance rate of the peptide SFLYEYSRR to hydroxyl free radicals can reach 46.49%, the inhibition rate of the peptide SFLYEYSRR to alpha-glucosidase can reach 64.36%, and the highest inhibition activity of the peptide SFLYEYSRR to tyrosinase can reach 48.33%. The limitation of the existing single-function peptide in application is overcome.

The invention belongs to the technical field of food science and bioengineering, and provides a method for improving antioxidant and hypoglycemic activity of bovine bone enzymatic hydrolysate. The method comprises the following steps: performing ultrasonic pretreatment on cattle bone powder (500W, 30min), performing alkaline protease hydrolysis (50 DEG C, 5h), mixing the inactivated cattle bone powder with D-xylose (8: 1), and performing Maillard reaction (100 DEG C, 120min). The DPPH free radical scavenging rate and the ABTS free radical scavenging rate of the obtained product respectively reach 91% and 88.13%, and the antioxidant activity is remarkably improved; the synthetic peptide, such as FGFDGDFYR, has both the hydroxyl radical scavenging rate of 74.52% and the alpha-glucamylase inhibition rate of 70.2%, so that the double-function activity of resisting oxidation and reducing blood sugar is realized; meanwhile, the product is enhanced in palatable taste and reduced in bitter taste, is rich in meat flavor compounds (such as 2-methyl-3-furanmercaptan), has flavor and functional advantages, and promotes high-value utilization of bovine bones.

The invention belongs to the field of medicinal chemistry, and discloses an application of a ferrous ion complex of dihydrochalcone compounds (phlorizin, PHL) and trilobatin, TLB) in prevention and treatment of food-borne microorganisms, and a preparation method of the ferrous ion complex of the dihydrochalcone compounds (phlorizin, PHL) and the trilobatin, TLB). The phlorizin-ferrous ion complex and the trilobatin-ferrous ion complex have relatively good DPPH (1, 1-diphenyl-2-picrylhydrazyl) scavenging capacity and hydroxyl radical scavenging capacity; the phlorizin-ferrous ion complex and the trilobatin-ferrous ion complex can slow down oxidation of edible oil and fat; the phlorizin-ferrous ion complex and the trilobatin-ferrous ion complex have different inhibitory activities on the tested food-borne microorganisms; when the compound is combined with the cefdinir for use, the phlorizin-ferrous ion and trilobatin-ferrous ion complexes can synergistically promote the antibacterial effect of the cefdinir, and the use dosage of the cefdinir is reduced. The two have the potential of being further researched and developed into a novel edible antibacterial agent.

The invention relates to the technical field of microorganisms, in particular to lactobacillus paracasei Q7-12 with low temperature resistance and oxidation resistance and application of the lactobacillus paracasei Q7-12. The lactobacillus paracasei Q7-12 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, 1 # yard, West Beichen Road, Chaoyang District, Beijing, the preservation date is December 11, 2025, and the preservation number is CGMCC No.37037. The lactobacillus paracasei Q7-12 has the advantages that the lactobacillus paracasei Q7-12 can be used for preparing the lactobacillus paracasei Q7-12; the strain shows the fastest growth rate under the condition of 4 DEG C. The antioxidant capacity of the strain is superior to that of a commercial strain XF, the scavenging rate of DPPH free radicals exceeds 95%, the scavenging rate of hydroxyl free radicals is higher than 75%, and the strain also has acid resistance, cholate resistance and antibacterial activity and provides a basis for silage processing and yogurt fermentation production.

The invention discloses a peptide APRP with antihypertensive and antioxidant activity and a preparation method and application thereof, and belongs to the technical field of food science and biology. According to the invention, the short peptide APRP (the sequence is Ala-Pro-Arg-Pro) with potential antihypertensive activity is screened out, the peptide APRP has remarkable ACE inhibitory activity, the half inhibitory concentration (IC50) of the peptide APRP is 0.2892 mM, and the inhibition rate of the peptide APRP in an HK-2 cell model under the concentration of 1nM is as high as 72.85%. Molecular docking results show that APRP is stably combined with an ACE active center through hydrogen bonds, salt bridges and hydrophobic interaction, and conversion of angiotensin I is effectively blocked. The polypeptide APRP also has antioxidant activity, the DPPH free radical scavenging capacity reaches 9.14%, and the hydroxyl free radical scavenging rate reaches 5.1%. The invention provides a new candidate molecule for natural treatment with hypertension and oxidation resistance at the same time.

The application belongs to the technical field of environmental monitoring, and discloses a OH-HO2-RO2 multi-radical standard source generation method and device and application thereof, wherein different water vapor content wet air is controlled to enter a flow tube and receive 184.9nm ultraviolet irradiation to generate synchronous photolysis, and based on the real-time measured water vapor content, ultraviolet radiation intensity value and the absorption cross-section value of oxygen and water vapor at 184.9nm, the OH and HO2 concentrations generated in the flow tube are inversed; carbon monoxide or different kinds of volatile organic compounds are controlled to be added into the flow tube to react with OH radicals to generate stable concentrations of HO2 or RO2. 6 10 3 The method and device can generate specific concentrations of OH, HO2 and different kinds of RO2 on line, and the concentration of generated radicals can be adjusted according to requirements, the concentration range is 10​​

The present disclosure provides a Lactobacillus reuteri SJ-47 with a preservation number of CGMCC No. 16416 and an exopolysaccharide secreted by the Lactobacillus reuteri SJ-47 and a preparation method thereof, which comprises: a fermentation culture step: after the Lactobacillus reuteri SJ-47 is inoculated in MRS broth and subjected to fermentation culture treatment, the fermentation broth supernatant is obtained by sterilization and separation; a crude polysaccharide extraction step: the fermentation broth supernatant is concentrated and enriched, and then subjected to first alcohol precipitation, enzymatic deproteinization, second alcohol precipitation, sevage deproteinization, dialysis and freeze-drying treatment to obtain a crude polysaccharide extract. The exopolysaccharide of the Lactobacillus reuteri SJ-47 provided in the present application has high yield and excellent performance, can effectively scavenge DPPH free radicals and hydroxyl free radicals, has good antioxidant capacity, can improve the expression levels of SOD, CAT and GSH, can increase the expression levels of type I collagen and matrix metalloproteinase, and plays a certain regulating role on the cell aging-apoptosis pathway.

A Ligilactobacillus salivarius strain Lumine-102 having whitening and anti-oxidation functions, and the use thereof, relating to the technical field of microorganisms and the use thereof. The Ligilactobacillus salivarius Lumine-102 is preserved in the China General Microbiological Culture Collection Center with the accession number of CGMCC No. 29489. The Ligilactobacillus salivarius Lumine-102 can effectively inhibit the activity of tyrosinase under both in vitro and in vivo conditions; in addition, the Lumine-102 strain also has an excellent hydroxyl radical scavenging capability and a relatively high reduction capacity and thus exhibits a very strong anti-oxidation capacity. Therefore, the Ligilactobacillus salivarius strain Lumine-102 exhibits application prospects in the field of preparing skin care products having such as whitening, anti-oxidation and anti-aging functions.

This invention discloses a *Equisetum hyemale* polysaccharide, its preparation method, and its applications, belonging to the field of natural polysaccharide technology. Using *Equisetum hyemale* as raw material, the polysaccharide was extracted using a hot water ultrasonic-assisted extraction method. Through single-factor and orthogonal experiments, the optimal process was determined to be a material-to-liquid ratio of 1:20 g / mL, a temperature of 70℃, an ultrasonic power of 400 W, and an extraction time of 50 min. Under these conditions, the polysaccharide yield reached 13.05%. The crude polysaccharide was deproteinized using the Sevage method and purified by DEAE-52 cellulose column and Sephadex G-100 gel column to obtain purified *Equisetum hyemale* polysaccharide (DEPS) with homogeneous components. Physicochemical characterization showed that the DEPS had a total sugar content of 55.36%, a molecular weight of 67.61 kDa, and was composed of mannose, glucuronic acid, glucose, and fucose. It lacked a triple helix structure and contained typical polysaccharide functional groups and pyranose ring structures. In vitro experiments showed that DEPS exhibited the highest scavenging rates of DPPH, ABTS, and hydroxyl radicals, reaching 87.54%, 97.52%, and 97.5%, respectively, with its antioxidant activity showing a concentration-dependent effect. This invention features a simple process and high yield, producing a high-quality Equisetum hyemale polysaccharide.

The invention discloses a treatment method for preparing isomaltulose-containing syrup from citrus peel-removed collection liquid by an enzyme method and application, which comprises the following steps: removing citrus peel peel by enzymolysis, and preparing functional syrup from the obtained collection liquid as a raw material by an enzyme method, so that synchronous production of canned citrus and functional syrup from the citrus peel-removed collection liquid is realized; experimental results show that the DPPH free radical scavenging rate of the isomaltulose-containing syrup is 156.15%, the hydroxyl free radical scavenging rate of the isomaltulose-containing syrup is 83.33%, and the total antioxidant capacity of the isomaltulose-containing syrup is 23.75%; compared with a basic MRS culture medium, the prepared MRS culture medium taking the isomaltulose-containing syrup as the carbon source has a better probiotic proliferation effect on three probiotics including lactobacillus paracasei, streptococcus thermophilus and lactobacillus plantarum.