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92 results about "Peroxyl radical" patented technology

The hydroperoxyl radical, also known as the perhydroxyl radical, is the protonated form of superoxide with the chemical formula HO 2

Application of Hepu pearl polypeptide Nacrein-LP20 in preparation of medicine for promoting wound healing

The invention belongs to the technical field of biological medicines, and particularly relates to an application of a Pucurbita pepo polypeptide Nacrein-LP20 in preparation of a medicine for promoting wound healing and a preparation method of the Pucurbita pepo polypeptide Nacrein-LP20. The amino acid sequence of the Hepu pearl polypeptide Nacrein-LP20 is as shown in SEQ ID NO: 1 (Sequence Identity Number 1). According to the present invention, the Pucurbita pepo active polypeptide Nacrein-LP20 has characteristics of strong DPPH free radical and hydroxyl free radical removing ability and excellent antioxidant activity, can significantly promote HaCaT cell proliferation in the high glucose environment, and can promote migration and repair of HaCaT cells in the high glucose environment; the traditional Chinese medicine composition has a remarkable healing promoting effect on full-cortical resection wound surfaces of normal rats and diabetic rats.
Owner:GUANGXI UNIV OF CHINESE MEDICINE

DSH loaded medicine as well as preparation method and application thereof

The invention belongs to the technical field of biological medicines, and discloses a DSH loaded medicine as well as a preparation method and application thereof. The DSH loading type medicine is DNA (Deoxyribonucleic Acid) supramolecular hydrogel loaded with 2, 2, 6, 6-tetramethylpiperidine-1-oxide, and the DSH loading type medicine is a DNA supramolecular hydrogel loaded with the 2, 2, 6, 6- Wherein the DNA supramolecular hydrogel is formed by assembling a Y type DNA monomer and an L type DNA monomer. The DSH loaded medicine has good anti-inflammatory and peroxy free radical removing effects, and can achieve the purpose of treating knee osteoarthritis. In addition, the DSH loaded medicine can be used for reflecting the content of peroxy free radicals in knee joints by detecting the contrast ratio of knee joint images through magnetic resonance, and early diagnosis of knee osteoarthritis is achieved.
Owner:GUANGZHOU FIRST PEOPLES HOSPITAL (GUANGZHOU DIGESTIVE DISEASE CENT GUANGZHOU FIRST PEOPLES HOSPITAL GUANGZHOU MEDICAL UNIV THE SECOND AFFILIATED HOSPITAL OF SOUTH CHINA UNIV OF TECH)

Ozone generation rate monitoring system and monitoring method

The invention is suitable for the technical field of pollutant monitoring, and provides an ozone generation rate monitoring system and method, the system comprises a zero gas generation module, a gas distribution module, a multi-mode combination switching valve, a free radical generation source, a chemical amplification cavity and a detection module; the gas distribution module is used for inputting standard reactant gas with different components and sample gas sample; the input end of the multi-mode combined switching valve is connected with the zero gas generation module and the gas distribution module; a first output end of the multi-mode combined switching valve is connected with a gas inlet of the free radical generation source, a second output end is connected with a gas inlet of the chemical amplification cavity, and a third output end is connected with a gas outlet of the chemical amplification cavity; an exhaust port of the free radical generation source is connected with an air inlet of the chemical amplification cavity; and the exhaust port of the chemical amplification cavity is also connected with the detection module. According to the method, the ozone photochemical generation rate is directly obtained by accurately measuring data such as the total peroxy free radical concentration, the detection precision is higher, and the speed is higher.
Owner:安徽蓝盾光电子股份有限公司

Lactobacillus plantarum and application thereof

The invention belongs to the technical field of microorganisms, and particularly relates to a lactobacillus plantarum (Lactobacillus plantarum) and an application of the lactobacillus plantarum. The preservation number of the lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) NO.24756. The lactobacillus plantarum has good DPPH (1, 1-diphenyl-2-picrylhydrazyl) scavenging capacity, hydroxyl radical scavenging capacity and reducing capacity, and can remarkably reduce the level of malondialdehyde (MDA) as an oxidative stress marker in serum and liver of an oxidative stress body, the pathological damage level of liver tissue and the level of inflammatory factors in serum; the level of an antioxidant substance glutathione (GSH) in serum and liver of an oxidative stress body and the activity of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in the serum and liver can be remarkably improved. In addition, fire extinguishing bacteria of the lactobacillus plantarum have more excellent antioxidant activity than viable bacteria.
Owner:CHONGQING TIANYOU DAIRY CO LTD

Lactobacillus plantarum fermentation liquor and application thereof

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus plantarum fermentation liquor and application thereof. The lactobacillus plantarum fermentation liquor provided by the invention is obtained by taking roses as a substrate and fermenting screened plant milk stalks under specific conditions. Experimental results show that the content of effective active ingredients such as total polyphenol, total polysaccharide and superoxide dismutase in rose fermentation liquor fermented by the lactobacillus plantarum is relatively high, and the fermentation effect of the rose fermentation liquor is far superior to that of commercially available common lactobacillus plantarum and other strains; besides, the rose fermentation liquor also shows obvious DPPH free radical and hydroxyl free radical scavenging ability, can effectively inhibit the activity of tyrosinase and improve the cell viability, has anti-aging and whitening effects, and can be widely applied to various foods and skin care products.
Owner:NINGXIA JIAHE HUAYU ECOLOGICAL AGRI CO LTD

Hepu pearl polypeptide Nacrein-LP20 for treating cataract and application of Hepu pearl polypeptide Nacrein-LP20

The invention discloses a Hepu pearl polypeptide Nacrein-LP20 for treating cataract and application of the Hepu pearl polypeptide Nacrein-LP20, and belongs to the technical field of biological medicine. The amino acid sequence of the gene is as shown in SEQ ID NO. 1. The active polypeptide Nacrein-LP20 provided by the invention has strong capability of removing DPPH free radicals and hydroxyl free radicals, and has a certain application value in the aspect of oxidation resistance. The active polypeptide Nacrein-LP20 provided by the invention has a remarkable protective effect on oxidative stress of human lens cells, can improve the cataract lesion of selenium rats, and has a certain application value in the aspect of cataract treatment.
Owner:GUANGXI UNIV OF CHINESE MEDICINE

Casein-derived multifunctional peptide

The invention discloses a casein-sourced multifunctional peptide, and belongs to the field of food science and nutriology. The casein is subjected to enzymolysis through pepsin, a method combining activity verification and mass spectrum identification is adopted, the novel multifunctional peptide YPE with a clear sequence is screened and verified, the highest ABTS clearance rate of the casein peptide YPE can reach 77.76%, the highest hydroxyl radical clearance rate of the casein peptide YPE can reach 40.18%, and the casein peptide YPE can be used for preparing the novel multifunctional peptide YPE. The inhibition rate of alpha-glucosidase reaches 30.47%, the inhibition rate of escherichia coli is 10.18%, and the limitation of an existing single-function peptide in application is overcome.
Owner:NINGBO UNIV

Preparation method and application of high-activity antioxidant product based on hericium erinaceus-hawthorn solid state fermentation

The invention belongs to the technical field of biological fermentation, and particularly relates to a preparation method and application of a high-activity antioxidant product based on hericium erinaceus-hawthorn solid state fermentation. Activating the strain to prepare a seed solution; adding hawthorn into the rice solid culture medium, inoculating hericium erinaceus, performing constant-temperature culture, drying a fermentation product to constant weight at normal temperature and normal pressure after the culture is finished, crushing, and sieving for later use. The antioxidant effect of the fermentation product is comprehensively evaluated and verified through a systematic in-vitro chemical antioxidant experiment (ABTS < + >, DPPH free radical and hydroxyl free radical scavenging) and an in-vivo zebra fish oxidative stress model, and solid data support is provided for application of the fermentation product in healthy products. The resource high-value utilization way of the hawthorn and the hericium erinaceus is widened, and a technical scheme and a product prototype are provided for developing novel food raw materials, health-care products or medicine auxiliary materials with definite antioxidant functions.
Owner:SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE

Low-molecular-weight enteromorpha polysaccharide with antioxidant activity and preparation method thereof

The invention discloses low-molecular-weight enteromorpha polysaccharide with antioxidant activity and a preparation method of the low-molecular-weight enteromorpha polysaccharide, and belongs to the technical field of polysaccharide preparation. The preparation method comprises the following steps: taking an enteromorpha crude polysaccharide solution, adding H2O2 to enable the concentration of the enteromorpha crude polysaccharide solution to be 0.04-0.08 M, and degrading for 90-120 minutes under ultraviolet rays to obtain the low-molecular-weight enteromorpha polysaccharide, the low-molecular-weight enteromorpha polysaccharide is composed of monosaccharides of rhamnose, glucose, galactose, xylose, mannose and arabinose according to the ratio of 1.08: 1.00: 0.83: 0.58: 0.69: 0.06, the scavenging capacity and the iron reducing capacity of DPPH, ABTS and hydroxyl free radicals are improved, and the low-molecular-weight enteromorpha polysaccharide is obtained. The compound has excellent antioxidant activity and can be used as or used for preparing an antioxidant active preparation. The preparation method disclosed by the invention is efficient and stable, can be used for preparing the low-molecular-weight enteromorpha polysaccharide with lighter color, lower molecular weight and higher antioxidant activity, and has higher potential application value.
Owner:OCEAN UNIV OF CHINA

Metal organic coordination network material as well as preparation method and application thereof

PendingCN119139259Agood choice toxicityhigh activityOrganic active ingredientsAntinoxious agentsTumour metabolismPhosphorylation
A preparation method of the metal organic coordination network comprises the following steps: respectively dissolving copper acetate and polyphenol in deionized water or dimethyl sulfoxide to form a copper acetate solution and a polyphenol solution, then dropwise adding the copper acetate solution into the polyphenol solution, introducing nitrogen, and carrying out a heating reaction to obtain a reaction mixture; centrifuging the reaction mixture to obtain a precipitation product metal organic coordination network; the metal organic coordination network is applied to preparation of a material for targeted killing of hypoxic tumor cells. The metal organic coordination network disclosed by the invention is good in dispersity, high in biocompatibility and good in hypoxic tumor cell selective toxicity. After being degraded by glutathione, the glutathione derivative has good peroxidase activity, can catalyze hydrogen peroxide to generate hydroxyl free radicals with stronger cytotoxicity, and can be used for converting tumors into oxidative phosphorylation metabolic pathways by inhibiting hypoxic tumor glycolysis and remodeling hypoxic tumor metabolism, promoting tumor cells to generate ROS (reactive oxygen species), and further improving the activity of glutathione. And the sensitivity of hypoxic tumors to copper death is enhanced.
Owner:XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV

Composition with anti-oxidation effect as well as preparation method and application thereof

The invention provides an agropyron cristatum-Chinese date antioxidant composition as well as a preparation method and application thereof, and belongs to the technical field of functional foods. The composition consists of wheatgrass and Chinese dates. The composition provided by the invention can be used for removing hydroxyl radicals and exerting an anti-oxidation effect. The hydroxyl radical scavenging rate of the composition is obviously higher than that of single wheatgrass or single jujube, and the composition shows a synergistic anti-oxidation effect.
Owner:TIANJIN LIUYEFENG INTELLIGENT CHRONIC DISEASE MEDICAL TECHNOLOGY CO LTD +1

Acetylated raffinose and application thereof

The invention belongs to the technical field of biology, and particularly relates to acetylated raffinose and application thereof. According to the invention, acetylation modification is carried out on raffinose by using an acetylation reagent to obtain acetylated raffinose, the acetylated raffinose can significantly improve the scavenging ability and total antioxidant ability of raffinose on DPPH free radicals, and the scavenging ability on hydroxyl free radicals and the reduction ability on iron ions are also improved to a certain extent. The acetylated raffinose provided by the invention has potential application value in development of functional foods and antioxidants.
Owner:XINJIANG AGRI UNIV

Method for extracting nanocrystallized bamboo fiber cell walls from bamboo green

The invention discloses a method for extracting nanocrystallized bamboo fiber cell walls from bamboo green, which comprises the following steps: thermally activating a bamboo green material from natural bamboo wood in a weak base solution, and then carrying out organic acid mediated in-situ peroxy radical oxidation by using an organic acid-hydrogen peroxide mixture aqueous solution, then the water suspension is subjected to hydraulic separation under the hydraulic action to obtain bamboo fiber cells, and finally the obtained bamboo fiber cells are subjected to mechanical crushing and nanocrystallization refining treatment to obtain the needed nanocrystallization bamboo fiber cell walls. According to the method for extracting the nanocrystallized bamboo fiber cell wall, the natural moso bamboo is directly used as the raw material, the bamboo does not need to be pulped or smashed in advance, the preparation process is simple, the method is suitable for large-scale production, and the obtained nanocrystallized bamboo fiber cell wall product can be widely applied to the fields of food thickening, low-GI food additives and the like.
Owner:UNIV OF SCI & TECH OF CHINA

Fermentation culture medium for high yield of oudemansiella oosporium mycelium polysaccharide and culture method thereof

PendingCN121227832AFungiMicroorganism based processesBiotechnologyOudemansiella
The invention discloses a fermentation medium for high yield of oudemansiella oosporium mycelium polysaccharide and a culture method thereof. The fermentation medium comprises the following components in percentage by mass: 20-25% of potatoes, 1-4% of corn flour, 0.2-0.4% of peptone, 0.2-0.4% of CaSO4, 0.05-0.15% of MgSO4 and the balance of water, and the pH value of the fermentation medium is 5.5-6.5. According to the culture medium, growth of Oudemansiella ovalis hyphae can be accelerated by regulating and controlling the proportion of different nutritional components, synthesis of Oudemansiella ovalis polysaccharide can be promoted, the final sugar yield of the strain is increased by 7 times compared with that of conventional culture by adopting the optimized fermentation culture medium and fermentation process, and the sugar yield can reach 167.37 mg / g. The scavenging rates of DPPH free radicals, ABTS free radicals and hydroxyl free radicals of the oospore oudemansiella sp. Polysaccharide are 95.02%, 98.08% and 93.74% respectively, and the scavenging rates are obviously higher than those of previous research results. The invention provides a new direction for developing natural antioxidants, and also provides theoretical significance and application value for developing oudemansiella oosporium mycelium fermentation products.
Owner:YANGTZE NORMAL UNIVERSITY

Application of a porcine-derived Bacillus in antioxidant activity

This invention discloses the application of a porcine-derived Bacillus in antioxidant activity, wherein the porcine-derived Bacillus is used to prepare antioxidant products. The porcine-derived Bacillus is Bacillus subtilis YB-X-721, classified as Bacillus subtilis, and was deposited at the China General Microbiological Culture Collection Center on November 10, 2023, with accession number CGMCC No. 28942. The fermentation supernatant, bacterial suspension, and cell-free extract of the porcine-derived Bacillus subtilis YB-X-721 of this invention all possess certain DPPH free radical, superoxide anion free radical, and hydroxyl free radical scavenging abilities, as well as a certain reducing ability, and can be used to prepare antioxidant products. Furthermore, when the porcine-derived Bacillus subtilis YB-X-721 of this invention is added to piglet feed as an animal feed additive, it can improve the growth performance of weaned piglets, significantly increasing average daily weight gain, and significantly reducing average daily feed intake, feed conversion ratio, and diarrhea rate.
Owner:NAT CENT OF TECH INNOVATON FOR PIGNS +1

Method for extracting roselle anthocyanin through enzymolysis-ultrasonic assistance, product and application of product

The invention relates to a method for extracting roselle anthocyanin through enzymolysis-ultrasonic assistance, a product and application of the product. The method comprises the following steps: degreasing roselle powder, and performing enzymolysis for 24 hours by using cellulase and pectinase (20U / g respectively) under the conditions that the pH is 5.0 and the temperature is 40 DEG C; an acetone-hydrochloric acid mixed solution (the volume fraction of hydrochloric acid is 36%) with the liquid-material ratio being 10: 1 serves as an extracting agent, and ultrasonic extraction is conducted for 34 minutes at the temperature of 58 DEG C and the power of 160 W; and finally, centrifuging, extracting with ethyl acetate, and purifying to obtain an anthocyanin product. According to the method, the extraction rate reaches 3.087 mg / g, the main component of the product is cyanidin-3-glucoside, the antioxidant and antibacterial activity is remarkable, the scavenging rates of DPPH, ABTS and hydroxyl radicals are 86.26%, 81.34% and 93.75% respectively under the concentration of 3.0 mg / mL, and the scavenging rates are superior to those of vitamin C with the same concentration; under the concentration of 11mg / mL, the compound has an obvious inhibition effect on escherichia coli and staphylococcus aureus. The process is efficient and mild, the product activity is excellent, and the method is suitable for development and application of natural antioxidants and antibacterial agents.
Owner:COLLEGE OF SCI & TECH NINGBO UNIV

Probiotic transformed sea cucumber blanching liquid metagen with anti-oxidation effect as well as preparation method and application of probiotic transformed sea cucumber blanching liquid metagen

The invention discloses a probiotic transformed sea cucumber blanching liquid metagen with an anti-oxidation effect, and a preparation method and application thereof, and belongs to the technical field of food ingredients. The preparation method comprises the following steps: inoculating a probiotic liquid into sea cucumber blanching liquid according to the volume fraction of 1%-6%, and then fermenting for 12-120 hours at the temperature of 31-43 DEG C; and after fermentation is completed, concentrating the fermentation liquor to 5-25% of the original volume, and sterilizing to obtain the feed additive. According to the method, the sea cucumber blanching liquid is used as the only substrate for probiotic fermentation to prepare the postbiotics with the anti-oxidation effect, the preparation method does not need to additionally add a nitrogen source and a carbon source, fermentation liquid can be enriched without separation, operation is easy, energy consumption is reduced, and ecological and environment-friendly effects are achieved. The metagen provided by the invention has a relatively good scavenging capability on ABTS cation free radicals, DPPH free radicals and hydroxyl free radicals, so that the metagen has a very good application prospect in preventing oxidative deterioration of foods.
Owner:QINGDAO AGRI UNIV +3

Cleome gynandra extract as well as preparation method and application thereof

The invention provides a cleome gynandra extract as well as a preparation method and application thereof, and belongs to the technical field of cleome gynandra extraction. The preparation method comprises the following steps: mixing cleome gynandra leaves with an extraction solvent, performing ultrasonic extraction, filtering and collecting filtrate, centrifugally collecting supernate, concentrating and freeze-drying to obtain the cleome gynandra extract, the extraction solvent is water, an ethanol solution with the volume fraction of 43-47% or an ethanol solution with the volume fraction of 88-92%. Research finds that the cleome gynandra extract extracted by using water, an ethanol solution with the volume fraction of 43-47% or an ethanol solution with the volume fraction of 88-92% has obvious DPPH and hydroxyl radical scavenging rate, the death rate and hatching rate of zebra fish can be increased, zebra fish nrf2 signal channel related genes are influenced, the oxidative stress degree is reduced, and the zebra fish nrf2 signal channel related genes can be inhibited. Further, an obvious protection effect on hydrogen peroxide induced oxidative stress zebrafish is achieved. The preparation method is simple, easy to operate and suitable for industrial production.
Owner:HUBEI NORMAL UNIV

A method for extracting polysaccharides from *Micrococcus pluvialis* and its applications

This invention discloses a method for extracting polysaccharides from *Microcystis aeruginosa* and its applications. The method includes fat removal, alcohol-soluble molecule removal, ultrasonic water extraction, flavonoid removal, protein removal, pigment removal, ethanol precipitation, small molecule substance removal, and freeze-drying. This invention combines directional stepwise impurity removal with ultrasonic water extraction to achieve efficient separation of polysaccharides from impurities. The optimal ultrasonic extraction process was determined using response surface methodology: a fixed ultrasonic extraction power of 100W, an ultrasonic extraction temperature of 49℃, an extraction time of 61 min, a solid-liquid ratio of 1:20.5 g / mL, and three extractions. Under these conditions, the polysaccharide yield reached 1.45%. The obtained *Microcystis aeruginosa* polysaccharides exhibit significant antioxidant activity, showing good scavenging effects against DPPH free radicals, hydroxyl free radicals, and ABTS free radicals, with the total reducing power increasing in a dose-dependent manner. This invention provides a simple and controllable extraction process, yielding polysaccharides with high purity and good activity, which can be used as natural antioxidants in functional foods, health products, and cosmetics.
Owner:GUANGXI AI RUIKE INTERNATIONAL TRADE CO LTD

Functional saccharomyces cerevisiae and application thereof in fermentation of pueraria polysaccharide

The invention provides functional saccharomyces cerevisiae and application thereof in fermentation of pueraria polysaccharide, and belongs to the technical field of microbial fermentation. The invention discloses a saccharomyces cerevisiae Y25, which has the physiological characteristics of high ethanol yield, low higher alcohol yield, high ester yield and good tolerance to ethanol, acid and high temperature, can improve the quality of white spirit and enrich and coordinate the flavor of the white spirit, and can be used for fermenting kudzuvine root polysaccharide, so that the obtained fermented polysaccharide is high in yield, strong in oxidation resistance and high in yield. The polysaccharide content is increased by 9.79% compared with commercial Angel yeast, and the DPPH free radical scavenging rate and the hydroxyl free radical scavenging rate are respectively increased by 10.32% and 18.78% compared with the commercial Angel yeast. The saccharomyces cerevisiae Y25 disclosed by the invention has far-reaching social and economic significance on enhanced utilization of functional brewing microbial resources and innovation and upgrading of a traditional brewing process.
Owner:中原食品实验室

Hepu Pearl Polypeptide Nacrein-LP20 for Treating Cataract and Its Application

The present invention discloses a Hepu pearl polypeptide Nacrein-LP20 for treating cataracts and its application, belonging to the technical field of biomedicine. Its amino acid sequence is shown in SEQ ID NO.1. The active pearl polypeptide Nacrein-LP20 provided by the present invention has strong ability to scavenge DPPH free radicals and hydroxyl free radicals, and has certain application value in antioxidant aspect. The active pearl polypeptide Nacrein-LP20 provided by the present invention has a significant protective effect on oxidative stress of human lens cells, can improve the cataract lesions of selenium-induced rats, and has certain application value in cataract treatment.
Owner:GUANGXI UNIV OF CHINESE MEDICINE

Antioxidant preparation combining probiotics and traditional Chinese medicinal materials as well as preparation method and application of antioxidant preparation

The invention belongs to the technical field of antioxidant products, and particularly relates to an antioxidant preparation combining probiotics and traditional Chinese medicinal materials as well as a preparation method and application of the antioxidant preparation. The antioxidant preparation combining the probiotics and the traditional Chinese medicinal materials is a mixture obtained by standing a mixed solution of radix paeoniae alba powder, bifidobacterium and water for 18-24 hours and adding sodium chloride, the mass ratio of the white peony root powder to the water is (5-15): 1000, the concentration of the bifidobacteria in the mixed solution is more than 107 pieces / mL, and the mass fraction of the sodium chloride in the mixture is 0.8%-0.9%. The antioxidant preparation disclosed by the invention has the capability of removing superoxide anion free radicals, hydroxyl free radicals, peroxynitroso anion free radicals, hydrogen peroxide free radicals, chlorine free radicals and nitrate free radicals, can improve the activity of superoxide dismutase and glutathione peroxidase, and comprehensively shows relatively strong antioxidant capability; the application prospect is good.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY

Multifunctional active peptide from mackerel viscera and application thereof

The invention discloses a multifunctional active peptide from mackerel viscera and application of the multifunctional active peptide. The mackerel viscera extract is prepared by adopting a single exogenous enzyme hydrolysis method, the extract has small molecule peptides with ACE inhibitory activity, three ACE inhibitory peptides are obtained by further identification and screening, the amino acid sequences are respectively GPPVPGP, GPMGPMGP and SPGPVGMP, and the half inhibitory concentration IC50 values of the ACE inhibitory peptides are respectively 80.84 + / -1.12 mu g / mL, 177.41 + / -3.26 mu g / mL and 163.25 + / -3.46 mu g / mL. Wherein the heptapeptide GPPVPGP with the optimal activity also has the function of removing DPPH free radicals and hydroxyl free radicals, and can be used for adjuvant therapy for reducing hypertension or applied to other fields.
Owner:QUANZHOU NORMAL UNIV

Leech polypeptide mutant with high anticoagulation, oxidation resistance and saccharification resistance and preparation method of leech polypeptide mutant

The invention discloses a recombinant leech polypeptide with high anticoagulant activity as well as a preparation method and application thereof. A PelB-T2Y-N47K-N52Y mutant is constructed by carrying out structural optimization on a hirudin HV2 sequence, a polypeptide product with relatively high anticoagulant activity is obtained after induced expression and nickel affinity chromatography purification are carried out in a prokaryotic expression system, and the anticoagulant activity of the polypeptide product reaches 9135.8 ATU / mg and is remarkably superior to that of an initial sequence HV2. Meanwhile, the polypeptide shows a lower IC50 value in a hydroxyl radical scavenging experiment, and has the activity of inhibiting generation of AGEs in a non-enzymatic saccharification model. Molecular docking and binding free energy analysis show that the combination of the mutant and thrombin (PDB ID: 4HTC) is more stable. The leech polypeptide disclosed by the invention has the potential of being developed into a multifunctional bioactive peptide preparation, and is suitable for the related fields of anticoagulation, antioxidation and anti-saccharification.
Owner:EAST CHINA UNIV OF SCI & TECH

Alpha-lactalbumin multifunctional peptide with antioxidant, anticoagulant and tyrosinase inhibitory activities and application thereof

The application discloses a kind of alpha-lactalbumin multifunctional peptides with antioxidant, anticoagulant and tyrosinase inhibitory activity and application thereof, and belongs to the field of food, biopharmaceutical and cosmetic technology.The multifunctional peptide SEKLDQWLCEKL is screened, and the DPPH clearance rate and ABTS clearance rate of the multifunctional peptide SEKLDQWLCEKL are highest, which can reach 62.32% and 88.32% respectively, the clearance rate of hydroxyl radical is highest, which can reach 78.89%, PT and TT are respectively extended by 3.4 s and 4.7 s, and the inhibition rate of tyrosinase is highest, which can reach 89.07%, which overcomes the limitation of existing single functional peptide in application.
Owner:NINGBO UNIV

Hemerocallis fulva polysaccharide DLP-N as well as preparation method and application thereof

The invention relates to the technical field of active polysaccharide preparation, in particular to a hemerocallis fulva polysaccharide DLP-N as well as a preparation method and application of the hemerocallis fulva polysaccharide DLP-N. Grinding the dried day lily into powder, degreasing, desugaring and air-drying; preparing a deep eutectic solvent; using an extraction deep eutectic solvent; performing alcohol precipitation; performing freeze-drying; removing protein by a Sevage method; depigmentation is performed; carrying out DEAE-52 cellulose anion exchange chromatography; and carrying out sephadex SephacrylS-400 chromatography to obtain the white flocculent refined polysaccharide. The day lily polysaccharide component with remarkable antioxidant and cancer-inhibiting double activities is prepared by taking day lily as a raw material, the yield of the day lily polysaccharide component is as high as 25.6%, high-value utilization of resources is effectively realized, and the day lily polysaccharide component has excellent scavenging performance on DPPH free radicals, ABTS free radicals, hydroxyl free radicals and superoxide anion free radicals. Meanwhile, cancer cell proliferation can be effectively inhibited, and a definite structure-function relationship and functional gradient are shown.
Owner:SHANXI FUNCTIONAL FOOD RES INST OF SHANXI AGRI UNIV

Method for preparing small-molecular hyaluronic acid by enzyme membrane reactor and application of small-molecular hyaluronic acid

The invention discloses a method for preparing micromolecular hyaluronic acid by an enzyme membrane reactor and application of the micromolecular hyaluronic acid. Wherein the small molecular weight hyaluronic acid comprises low molecular weight hyaluronic acid and hyaluronic acid oligosaccharide. According to the invention, the bioreactor and the membrane separation device are coupled, so that the one-step preparation of two kinds of micromolecular hyaluronic acid with different molecular weight distributions is realized, the molecular structure is complete, the preparation conditions are mild and efficient, and the method is easy to apply to industrial production. The micromolecular hyaluronic acid prepared by the method provided by the invention can effectively improve the capacity of removing DPPH free radicals and hydroxyl free radicals, has certain oxidation resistance, and can be used for improving the intestinal oxidative stress state and / or relieving intestinal inflammation.
Owner:SHANGHAI JIAOTONG UNIV

A peroxyl radical source generation and calibration system

This invention provides a peroxide free radical source generation and calibration system, relating to the field of pollutant monitoring technology. The system includes a free radical generation module and an online calibration module. The free radical generation module includes a main photolysis cavity, which generates ·H and ·OH by photolysis of H2O using ultraviolet light. The ·H generated in the main photolysis cavity reacts with O2 to generate HO2 free radicals. The online calibration module includes a calibration channel connected to the main photolysis cavity and a separate background channel. NO standard gas is introduced into the calibration channel for calibration; NO standard gas is added to the background channel as background. This invention can accurately acquire NO2 signals under both active and passive scenarios, effectively deducting background interference and achieving quantitative measurement of HO2.
Owner:安徽蓝盾光电子股份有限公司

Atmospheric Total Peroxyl Radical Monitoring System

ActiveCN309351460SNitrogen oxidesAutoanalysis
1. Name of the Design Product: Atmospheric Total Peroxyl Radical Monitoring System. 2. Use of the Design Product: For the automatic analysis of the contents of carbon monoxide, hydrogen sulfide, hydrogen, nitrogen oxides, sulfur dioxide, etc. in industries such as metallurgy, coal, electricity, and chemical engineering. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph that Best Illustrates the Design Key Points: Usage State Diagram.
Owner:安徽蓝盾光电子股份有限公司

Ganoderma lucidum polysaccharide efficient extraction method and function and application thereof

The invention discloses an efficient ganoderma lucidum polysaccharide extraction method and functions and application thereof. According to the method, efficient and green extraction of the ganoderan is achieved by virtue of a free radical-DES synergistic effect. According to the method, hydrogen peroxide is heated to generate hydroxyl radicals to damage ganoderma lucidum cell walls, the release efficiency of intracellular polysaccharide is improved, parameters such as hydrogen peroxide concentration and treatment time are optimized, and damage to a polysaccharide structure due to excessive oxidation is avoided. On the basis, the released polysaccharide is dissolved and extracted by utilizing the dissolving capacity of a deep eutectic solvent (DES), and the dissolving and extracting effects of the DES on the polysaccharide are fully exerted by optimizing parameters such as the type of the DES, the water content of hydrated DES, the treatment temperature and time. The ganoderma lucidum polysaccharide with high yield and strong biological activity is finally obtained by taking the ganoderma lucidum polysaccharide extraction yield as a response value and by virtue of a BBD design method and an SEM scanning electron microscope.
Owner:CHINA JILIANG UNIV