Antioxidant preparation combining probiotics and traditional Chinese medicinal materials as well as preparation method and application of antioxidant preparation

Abstract

The invention belongs to the technical field of antioxidant products, and particularly relates to an antioxidant preparation combining probiotics and traditional Chinese medicinal materials as well as a preparation method and application of the antioxidant preparation. The antioxidant preparation combining the probiotics and the traditional Chinese medicinal materials is a mixture obtained by standing a mixed solution of radix paeoniae alba powder, bifidobacterium and water for 18-24 hours and adding sodium chloride, the mass ratio of the white peony root powder to the water is (5-15): 1000, the concentration of the bifidobacteria in the mixed solution is more than 107 pieces / mL, and the mass fraction of the sodium chloride in the mixture is 0.8%-0.9%. The antioxidant preparation disclosed by the invention has the capability of removing superoxide anion free radicals, hydroxyl free radicals, peroxynitroso anion free radicals, hydrogen peroxide free radicals, chlorine free radicals and nitrate free radicals, can improve the activity of superoxide dismutase and glutathione peroxidase, and comprehensively shows relatively strong antioxidant capability; the application prospect is good.

Description

Technical Field

[0001] The present invention belongs to the technical field of antioxidant products, and particularly relates to an antioxidant preparation combining probiotics and traditional Chinese medicines, and a preparation method and application thereof. Background Art

[0002] During normal physiological processes, the human body continuously produces free radicals, such as superoxide anions and hydroxyl radicals. A moderate amount of free radicals contributes to physiological functions such as cell signaling and immune defense. However, when free radical production is excessive or the body's antioxidant capacity is insufficient, oxidative stress can occur, damaging cells and tissues and leading to the development of a variety of diseases. Antioxidant preparations can help scavenge excess free radicals, maintain redox balance in the body, and ensure normal physiological function. With aging, the body's antioxidant capacity gradually declines, leading to the accumulation of free radical damage, a major factor in aging. Antioxidant preparations can combat free radical damage, reduce cellular and tissue aging, slow the aging process, and improve quality of life. Furthermore, oxidative stress is closely linked to the development and progression of many chronic diseases, such as cardiovascular disease, cancer, neurodegenerative diseases, and diabetes. The development of antioxidant preparations can help prevent the onset of these diseases and play a supporting role in their treatment, mitigating oxidative damage and improving symptoms.

[0003] In summary, the research and development of antioxidant preparations is necessary.

[0004] Currently known antioxidant preparations include vitamin C, vitamin E, coenzyme Q10, and plant extracts, encompassing a wide range of products. However, because antioxidant activity occurs within a complex biological environment, it is subject to interference from various factors, such as interactions between enzyme systems and other biomolecules within the body, resulting in actual antioxidant effects that fall short of expectations. Furthermore, different antioxidants vary in their ability to scavenge different types of free radicals, making a single antioxidant ineffective in comprehensively and effectively scavenging a wide range of free radicals. Therefore, there is a need to enhance the antioxidant capacity of antioxidant preparations. Summary of the Invention

[0005] In order to solve the above technical problems, the present invention provides an antioxidant preparation combining probiotics and traditional Chinese medicines, as well as a preparation method and application thereof.

[0006] The present invention aims to provide an antioxidant preparation combining probiotics with Chinese medicinal materials, which is a mixture of white peony powder, bifidobacteria and water, which is left to stand for 18 to 24 hours and then added with sodium chloride to obtain a mixture; the mass ratio of white peony powder to water is 5 to 15:1000, the concentration of bifidobacteria in the mixture is 10 7 The mass fraction of sodium chloride in the mixture is 0.8% to 0.9%.

[0007] The antioxidant preparation of the probiotics combined with Chinese medicinal materials provided by the present invention is firstly freed from the effective substances by standing for 18h to 24h, and then sodium chloride is added to enhance the antioxidant capacity of white peony powder and bifidobacteria. The experimental results of the present invention show that when the mass ratio of white peony powder to water is 5 to 15:1000, the concentration of bifidobacteria in the mixed solution is 10 7 When the mass fraction of sodium chloride in the mixture is 0.8% to 0.9%, the antioxidant capacity is the strongest, and it can scavenge more types of free radicals and has a wider range of applications. Compared with other genera of probiotics, the present invention can significantly enhance the antioxidant capacity of Bifidobacterium by blanching powder and sodium chloride.

[0008] It should be noted that, since the present invention uses live bifidobacteria, it needs to be refrigerated at a low temperature of 4°C to 10°C to prevent excessive proliferation of bifidobacteria.

[0009] Preferably, in the antioxidant preparation combining probiotics with Chinese medicinal materials, the bifidobacterium is Bifidobacterium adolescentis or Bifidobacterium infantis, both of which are highly safe and widely available.

[0010] Preferably, in the antioxidant preparation combining probiotics and Chinese medicinal materials, the mass fraction of sodium chloride in the mixture is 0.85%.

[0011] Preferably, the antioxidant preparation of the probiotics combined with Chinese medicinal materials has a live bacteria concentration of bifidobacteria in the mixed solution of 10 7 / mL~10 10 pieces / mL.

[0012] Preferably, the method for preparing the antioxidant preparation combining probiotics and Chinese medicinal materials comprises:

[0013] preparing the white peony root into white peony root powder;

[0014] The white peony root powder, bifidobacteria and water are mixed, left to stand for 18 to 24 hours, and sodium chloride is added to obtain a mixture which is an antioxidant preparation combining probiotics and Chinese medicinal materials;

[0015] The mass ratio of white peony root and water is 5-15:1000, and the concentration of bifidobacteria in the mixture is 10 7 The mass fraction of sodium chloride in the water extract is 0.8% to 0.9%.

[0016] The above preparation method can meet the needs of both laboratory-scale preparation and large-scale industrial production. In addition, the blanched powder can adsorb bifidobacteria and effectively mix with them. When sodium chloride is subsequently added, it produces an adsorption effect with the blanched powder and bifidobacteria, forming a complex structure that facilitates the antioxidant activity.

[0017] Preferably, the particle size of the white peony root powder is 20nm to 100nm, specifically 20nm, 30nm, 40nm, 50nm, 60nm, 70nm, 80nm, 90nm, and 100nm. Nano-scale white peony root powder has a large surface area and strong adsorption capacity, and can also quickly combine with free radicals to enhance the effect of scavenging free radicals.

[0018] Preferably, the method for preparing the antioxidant preparation of the combination of probiotics and traditional Chinese medicines comprises adding auxiliary materials to the mixture to obtain a liquid preparation of the antioxidant preparation of the combination of probiotics and traditional Chinese medicines.

[0019] Preferably, the auxiliary material is at least one of an emulsifier, a dispersant, a thickener and a stabilizer.

[0020] Preferably, the emulsifier is Tween-80 or Span-80.

[0021] Tween-80 is produced by esterifying sorbitan and oleic acid, followed by an addition reaction with ethylene oxide. It exhibits excellent emulsifying, dispersing, and solubilizing properties. Exemplarily, the amount of Tween-80 added to the antioxidant formulation of the present invention is equivalent to 0.1% to 0.5% by weight of the mixture, more specifically 0.1%, 0.2%, 0.3%, 0.4%, and 0.5%.

[0022] Span-80 is made from dehydrated sorbitol esterified with oleic acid. Its strong lipophilicity allows it to effectively emulsify oil-soluble antioxidants, maintaining their stability in aqueous systems. It also exhibits excellent chemical stability and is not easily affected by acidic or alkaline conditions. For example, the amount of Span-80 added to the antioxidant formulation of the present invention is equivalent to 0.5% to 3% by weight of the mixture, with more specific amounts including 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.5%, 2.0%, 2.5%, and 3%.

[0023] Preferably, the dispersant is sodium tripolyphosphate, sodium hexametaphosphate, sodium pyrophosphate or polyethylene glycol.

[0024] Among them, sodium tripolyphosphate has good complexing effect, which can make the antioxidant uniformly dispersed in the system. Its addition amount in the antioxidant preparation of the present invention is equivalent to 0.01% to 0.1% by mass of the mixture, more specifically 0.01%, 0.05%, and 0.1%.

[0025] Sodium hexametaphosphate has strong dispersibility, allowing for uniform dispersion of antioxidant particles and improving their stability. It also has emulsifying properties and high viscosity, contributing to the stable presence of the antioxidant in the system. Its addition to the antioxidant preparation of the present invention is typically 0.01% to 0.05% by weight of the mixture, with specific amounts including 0.01%, 0.02%, 0.03%, 0.04%, and 0.05%.

[0026] Sodium pyrophosphate shares the common properties of polyphosphates, including emulsification and dispersibility, preventing fat oxidation. It also inhibits oxidation and fermentation at high pH values, indirectly acting as a protective antioxidant. Its addition to the antioxidant preparation of the present invention is typically 0.01% to 0.05% by weight of the mixture, with specific amounts including 0.01%, 0.02%, 0.03%, 0.04%, and 0.05%.

[0027] Polyethylene glycol contains multiple hydroxyl groups in its molecular structure, which can form hydrogen bonds with antioxidant molecules, further improving the dispersion stability of the antioxidant. Polyethylene glycol is added to the antioxidant preparation of the present invention in an amount equivalent to 0.01% to 0.1% by weight of the mixture, more specifically 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, and 0.1%.

[0028] Preferably, the thickener is carrageenan, xanthan gum, guar gum or sodium carboxymethyl tricellulose.

[0029] Among them, carrageenan can form gels with various substances, so that the antioxidant is better dispersed in the system. Its addition amount in the antioxidant preparation of the present invention is equivalent to 0.1% to 1% by mass of the mixture, more specifically 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, and 1%.

[0030] Xanthan gum has good stability and strong resistance to heat, acid, alkali, salt and enzymatic hydrolysis, and can maintain the stability of the system and the dispersibility of the antioxidant. The amount of xanthan gum added to the antioxidant preparation of the present invention is equivalent to 0.1% to 1% of the mass of the mixture, more specifically 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9% and 1%.

[0031] Guar gum can improve the viscosity and stability of the system and make the antioxidant uniformly dispersed. The amount of guar gum added to the antioxidant preparation of the present invention is equivalent to 0.5% to 1.2% of the mass of the mixture, more specifically 0.5%, 0.7%, 0.9%, 1.1%, and 1.2%.

[0032] Sodium carboxymethyl tricellulose can improve the viscosity and stability of the system and evenly disperse the antioxidant. Its addition amount in the antioxidant preparation of the present invention is equivalent to 0.05% to 0.1% by weight of the mixture, more specifically 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, and 0.1%.

[0033] Preferably, the stabilizer is pectin or gum arabic.

[0034] Pectin is a natural polysaccharide with excellent emulsifying and stability properties, effectively preventing aggregation or precipitation of the antioxidant in the system, thereby maintaining its uniform distribution. Pectin is added to the antioxidant preparation of the present invention in an amount equivalent to 0.1% to 1.0% by weight of the mixture, more specifically 0.1%, 0.3%, 0.5%, 0.7%, 0.9%, and 1.0%.

[0035] Gum arabic is a natural plant secretion with excellent emulsifying and stability properties, effectively preventing aggregation or precipitation of antioxidants in the system. It is added to the antioxidant preparation of the present invention in an amount equivalent to 0.5% to 1.0% by weight of the mixture, more specifically 0.5% or 1.0%.

[0036] The present invention also provides the use of the antioxidant preparation combining the above-mentioned probiotics with Chinese medicinal materials, wherein the antioxidant preparation refers to a product having the ability to scavenge free radicals and enhance the activity of superoxide dismutase and glutathione peroxidase.

[0037] Preferably, in the application of the antioxidant preparation combining probiotics with Chinese medicinal materials, the free radicals include at least one of superoxide anion free radicals, hydroxyl free radicals, peroxynitrite anion free radicals, hydrogen peroxide free radicals, chlorine free radicals and nitrate free radicals.

[0038] Compared with the prior art, the present invention has the following beneficial effects:

[0039] The antioxidant preparation of probiotics combined with Chinese medicinal materials provided by the present invention is a mixture obtained by standing a mixture of white peony powder, bifidobacteria and water for 18 to 24 hours and adding sodium chloride; the mass ratio of white peony powder to water is 5 to 15:1000, the concentration of bifidobacteria in the mixture is 10 7 The amount of the antioxidant is greater than 0.8% / mL, and the mass fraction of sodium chloride in the mixture is 0.8% to 0.9%. This antioxidant has the ability to scavenge superoxide anion free radicals, hydroxyl free radicals, peroxynitrite anion free radicals, hydrogen peroxide free radicals, chlorine free radicals, and nitrate free radicals, and can enhance the activity of superoxide dismutase and glutathione peroxidase, demonstrating strong and broad antioxidant capacity and promising application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 This is the inventive concept diagram of the present invention. DETAILED DESCRIPTION

[0041] In order to enable those skilled in the art to better understand and implement the technical solution of the present invention, the present invention is further described below with reference to specific embodiments.

[0042] In the description of the present invention, unless otherwise specified, all reagents used are commercially available and all methods used are conventional techniques in the art.

[0043] The inventive concept of the present invention refers to Figure 1 ,include:

[0044] (1) Preparation of antioxidant preparations combining probiotics and Chinese medicinal materials

[0045] (1.1) Provide an antioxidant preparation combining probiotics and Chinese medicinal materials, which is a mixture obtained by allowing a mixture of white peony powder, bifidobacteria and water to stand for 18 to 24 hours and then adding sodium chloride; the mass ratio of white peony powder to water is 5 to 15:1000, the concentration of bifidobacteria in the mixture is 10 7 The mixture contains more than 100mg of probiotics per mL, and the mass fraction of sodium chloride in the mixture is 0.8% to 0.9%. The probiotics, Bifidobacterium spp., are highly safe and have strong antioxidant capacity, which can scavenge a wider range of free radicals. Compared with other types of probiotics, the present invention significantly enhances the antioxidant capacity of Bifidobacterium by using blanched powder and sodium chloride.

[0046] (1.2) A method for preparing an antioxidant preparation combining probiotics and traditional Chinese medicines is provided, comprising:

[0047] preparing the white peony root into white peony root powder;

[0048] The white peony root powder, bifidobacteria and water are mixed, left to stand for 18 to 24 hours, and sodium chloride is added to obtain a mixture which is an antioxidant preparation combining probiotics and Chinese medicinal materials;

[0049] The mass ratio of white peony root and water is 5-15:1000, and the concentration of bifidobacteria in the mixture is 10 7 The mass fraction of sodium chloride in the water extract is 0.8% to 0.9%.

[0050] The blanched powder can adsorb bifidobacteria and effectively mix with bifidobacteria. When sodium chloride is subsequently added, it produces an adsorption effect with the blanched powder and bifidobacteria, forming a complex structure, which is beneficial for it to exert its antioxidant activity.

[0051] (2) Dosage form optimization

[0052] The present invention provides a method for preparing an antioxidant preparation combining probiotics and traditional Chinese medicines, which has longer-term stability and is convenient for market application. The method comprises: adding auxiliary materials to the mixture. The auxiliary materials include at least one of an emulsifier, a dispersant, a thickener, and a stabilizer.

[0053] Illustratively, the emulsifier may be at least one of the following: Tween-80 and Span-80.

[0054] Exemplarily, the dispersant is sodium tripolyphosphate, sodium hexametaphosphate, sodium pyrophosphate or polyethylene glycol.

[0055] Exemplarily, the thickener is carrageenan, xanthan gum, guar gum or sodium carboxymethyltricellulose.

[0056] Exemplarily, the stabilizer is pectin or gum arabic.

[0057] Next, some specific examples and control groups are provided to illustrate the antioxidant effect of the antioxidant preparation combining probiotics with Chinese medicinal materials of the present invention.

[0058] Example 1

[0059] An antioxidant preparation combining probiotics with traditional Chinese medicines is prepared by allowing a mixture of white peony root powder, bifidobacteria and water to stand for 18 hours and then adding sodium chloride to obtain a mixture; the mass ratio of white peony root powder to water is 5:1000, the concentration of bifidobacteria in the mixture is 10 7 The mixture contained 0.85% sodium chloride by mass. The bifidobacterium was purchased from Shanxi Xinnuo Biotechnology Co., Ltd., with a viable count of 10 billion cfu / g. The following examples and control groups also used the same source of Bifidobacterium adolescentis. The white peony root powder had a particle size of 20 nm.

[0060] Example 2

[0061] An antioxidant preparation combining probiotics with Chinese medicinal materials, comprising a mixture of white peony root powder, bifidobacteria and water, which is allowed to stand for 18 hours and then sodium chloride is added to obtain a mixture; the mass ratio of white peony root powder to water is 10:1000, the concentration of bifidobacteria in the mixture is 10 7 The mass fraction of sodium chloride in the mixture is 0.85%. The bifidobacterium is Bifidobacterium adolescentis. The particle size of the white peony root powder is 20 nm.

[0062] Example 3

[0063] An antioxidant preparation combining probiotics and Chinese medicinal materials, comprising a mixture of white peony root powder, bifidobacteria and water, which is allowed to stand for 18 hours and then sodium chloride is added to obtain a mixture; the mass ratio of white peony root powder to water is 15:1000, the concentration of bifidobacteria in the mixture is 10 7 The mass fraction of sodium chloride in the mixture is 0.85%. The bifidobacterium is Bifidobacterium adolescentis. The particle size of the white peony root powder is 20 nm.

[0064] Example 4

[0065] An antioxidant preparation combining probiotics with traditional Chinese medicines is prepared by allowing a mixture of white peony root powder, bifidobacteria and water to stand for 24 hours and then adding sodium chloride to obtain a mixture; the mass ratio of white peony root powder to water is 5:1000, the concentration of bifidobacteria in the mixture is 10 7 The mass fraction of sodium chloride in the mixture is 0.85%. The bifidobacterium is Bifidobacterium adolescentis. The particle size of the white peony root powder is 20 nm.

[0066] Example 5

[0067] An antioxidant preparation combining probiotics with traditional Chinese medicines is prepared by allowing a mixture of white peony root powder, bifidobacteria and water to stand for 18 hours and then adding sodium chloride to obtain a mixture; the mass ratio of white peony root powder to water is 5:1000, the concentration of bifidobacteria in the mixture is 10 7 The mass fraction of sodium chloride in the mixture is 0.8%. The bifidobacterium is Bifidobacterium adolescentis. The particle size of the white peony root powder is 20 nm.

[0068] Example 6

[0069] An antioxidant preparation combining probiotics with traditional Chinese medicines is prepared by allowing a mixture of white peony root powder, bifidobacteria and water to stand for 18 hours and then adding sodium chloride to obtain a mixture; the mass ratio of white peony root powder to water is 5:1000, the concentration of bifidobacteria in the mixture is 10 7 The mass fraction of sodium chloride in the mixture is 0.9%. The bifidobacterium is Bifidobacterium adolescentis. The particle size of the white peony root powder is 20 nm.

[0070] Example 7

[0071] An antioxidant preparation combining probiotics with traditional Chinese medicines is prepared by allowing a mixture of white peony root powder, bifidobacteria and water to stand for 18 hours and then adding sodium chloride to obtain a mixture; the mass ratio of white peony root powder to water is 5:1000, the concentration of bifidobacteria in the mixture is 10 7 The mixture contains 0.9% sodium chloride. The bifidobacterium is bifidobacterium adolescentis. The white peony root powder has a particle size of 100 nm.

[0072] Example 8

[0073] An antioxidant preparation combining probiotics with traditional Chinese medicines is prepared by allowing a mixture of white peony root powder, bifidobacteria and water to stand for 18 hours and then adding sodium chloride to obtain a mixture; the mass ratio of white peony root powder to water is 5:1000, the concentration of bifidobacteria in the mixture is 10 7 The mass fraction of sodium chloride in the mixture is 0.85%. The bifidobacterium is Bifidobacterium adolescentis. The particle size of the white peony root powder is 10 μm.

[0074] Example 9

[0075] An antioxidant preparation combining probiotics with traditional Chinese medicines is prepared by allowing a mixture of white peony root powder, bifidobacteria and water to stand for 18 hours and then adding sodium chloride to obtain a mixture; the mass ratio of white peony root powder to water is 5:1000, the concentration of bifidobacteria in the mixture is 10 7 The mass fraction of sodium chloride in the mixture is 0.85%. The bifidobacterium is infantis bifidobacterium (purchased from Shaanxi Linzhou Biotechnology Co., Ltd., with a viable count of 10 billion cfu / g in the raw material). The particle size of the white peony root powder is 20 nm.

[0076] Control group setting:

[0077] Control group 1: A mixture of white peony root powder, Lactobacillus casei and water was allowed to stand for 18 hours and then sodium chloride was added; the mass ratio of white peony root powder to water was 5:1000, and the concentration of Lactobacillus casei in the mixture was 10 7 The mass fraction of sodium chloride in the mixture was 0.85%. Lactobacillus casei was purchased from Shandong Jiacheng Biotechnology Co., Ltd., with a viable count of 10 billion cfu / g. The particle size of white peony root powder was 20 nm. The antioxidant capacity of different probiotics combined with traditional Chinese medicines was compared.

[0078] Control group 2: A mixture of white peony root powder, bifidobacteria and water was allowed to stand for 18 hours to obtain a mixture; the mass ratio of white peony root powder to water was 5:1000, and the concentration of bifidobacteria in the mixture was 10 7 The bifidobacterium was Bifidobacterium adolescentis. The white peony root powder had a particle size of 20 nm. No sodium chloride was added to this control group.

[0079] Control group 3: A mixture of white peony root powder and water was allowed to stand for 18 hours to obtain a mixture; the mass ratio of white peony root powder to water was 5:1000. The white peony root powder had a particle size of 20 nm. This control group contained only white peony root powder.

[0080] Control group 4: a mixture of bifidobacteria and water was allowed to stand for 18 hours to obtain a mixture; the concentration of bifidobacteria in the mixture was 10 7 The bifidobacterium was Bifidobacterium adolescentis. The white peony root powder had a particle size of 20 nm. This control group was supplemented with only Bifidobacterium adolescentis.

[0081] Control group 5: sodium chloride solution with a mass fraction of 0.85%.

[0082] Control group 6: deionized water, which is a blank control.

[0083] The present invention illustrates the difference in antioxidant capacity of antioxidants through the following experiments.

[0084] 1. Effects of different antioxidants on free radical scavenging efficiency

[0085] (1) Superoxide anion free radical

[0086] Superoxide anion radicals are primarily generated by electron leakage from the mitochondrial respiratory chain. For example, during electron transfer in the cytochrome oxidase complex, some electrons leak onto oxygen molecules, generating superoxide anion radicals. Ultraviolet radiation, radiation, and heavy metal ions (such as iron and copper) can also promote their generation. Superoxide anion radicals have strong oxidizing properties and can damage biological membranes, proteins, and DNA. They can further react to generate more toxic free radicals, such as hydroxyl radicals.

[0087] The experimental method of superoxide anion free radical scavenging was referred to “Zhang Xiaoqiang, Pu Yuepu, Yin Lihong, et al. Experimental study on the scavenging effect of Cordyceps sinensis and artificial Cordyceps mycelium on superoxide anion free radical and hydroxyl free radical [J]. Chinese Journal of Gerontology, 2003, 23(11): 3. DOI: 10.3969 / j.issn.1005-9202.2003.11.028”.

[0088] Experimental results:

[0089] The superoxide anion radical scavenging rates of Examples 1 to 9 and Control Groups 1 to 6 are as follows: Example 1 is 75%, Example 2 is 78%, Example 3 is 72%, Example 4 is 78%, Example 5 is 74%, Example 6 is 73%, Example 7 is 75%, Example 8 is 65%, Example 9 is 76%, Control Group 1 is 35%, Control Group 2 is 52%, Control Group 3 is 40%, Control Group 4 is 45%, Control Group 5 is 0%, and Control Group 6 is 0%.

[0090] (2) Hydroxyl radicals

[0091] Hydroxyl radicals are produced by the Fenton reaction or radiolysis, for example, water molecules decompose under the action of ionizing radiation (such as X-rays and gamma rays) to produce hydroxyl radicals.

[0092] The experimental method for scavenging hydroxyl radicals was referred to “Zhang Xiaoqiang, Pu Yuepu, Yin Lihong, et al. Experimental study on the scavenging effects of Cordyceps sinensis and artificial Cordyceps mycelium on superoxide anion radicals and hydroxyl free radicals [J]. Chinese Journal of Gerontology, 2003, 23(11): 3. DOI: 10.3969 / j.issn.1005-9202.2003.11.028”.

[0093] Experimental results:

[0094] The superoxide anion radical scavenging rates of Examples 1 to 9 and Control Groups 1 to 6 are as follows: 80% for Example 1, 82% for Example 2, 81% for Example 3, 88% for Example 4, 81% for Example 5, 80% for Example 6, 81% for Example 7, 68% for Example 8, 78% for Example 9, 30% for Control Group 1, 51% for Control Group 2, 40% for Control Group 3, 41% for Control Group 4, 0% for Control Group 5, and 0% for Control Group 6.

[0095] (3) Peroxynitrite anion free radical

[0096] Nitric oxide (NO) and superoxide anion (O2 - ) reacts to generate peroxynitrite anion free radicals. Peroxynitrite anion free radicals are highly oxidizing and electrophilic, and can damage cell membranes, proteins, and DNA, inducing cell apoptosis.

[0097] The experimental method for scavenging peroxynitrite anion free radicals was referred to “Jiang Weizhe, Qu Kai, Zhu Haibo. Blood lipid regulation and antioxidant activity of Cordyceps sinensis extract [J]. Chinese Journal of Experimental Traditional Chinese Medicine, 2011, 17(12): 5. DOI: 10.3969 / j.issn.1005-9903.2011.12.037”.

[0098] Experimental results:

[0099] The superoxide anion radical scavenging rates of Examples 1 to 9 and Control Groups 1 to 6 are as follows: Example 1 is 76%, Example 2 is 80%, Example 3 is 80%, Example 4 is 78%, Example 5 is 76%, Example 6 is 80%, Example 7 is 79%, Example 8 is 70%, Example 9 is 79%, Control Group 1 is 31%, Control Group 2 is 50%, Control Group 3 is 38%, Control Group 4 is 38%, Control Group 5 is 0%, and Control Group 6 is 0%.

[0100] (4) Hydrogen peroxide free radical

[0101] During cellular metabolism, redox reactions in organelles such as mitochondria, peroxisomes, and the endoplasmic reticulum generate hydrogen peroxide free radicals. Ultraviolet radiation, radiation, and chemicals (such as phenolic compounds) can also promote their production. Hydrogen peroxide itself has a weak oxidizing property, but in the presence of catalysts such as iron ions, it can generate hydroxyl radicals, which can damage biomolecules.

[0102] The experimental method for scavenging hydrogen peroxide free radicals was referred to “Jiang Weizhe, Qu Kai, Zhu Haibo. Blood lipid regulation and antioxidant activity of Cordyceps sinensis extract [J]. Chinese Journal of Experimental Traditional Chinese Medicine, 2011, 17(12): 5. DOI: 10.3969 / j.issn.1005-9903.2011.12.037”.

[0103] Experimental results:

[0104] The superoxide anion radical scavenging rates of Examples 1 to 9 and Control Groups 1 to 6 are as follows: 80% for Example 1, 80% for Example 2, 79% for Example 3, 80% for Example 4, 77% for Example 5, 78% for Example 6, 80% for Example 7, 64% for Example 8, 80% for Example 9, 50% for Control Group 1, 55% for Control Group 2, 39% for Control Group 4, 40% for Control Group 5, 0% for Control Group 6, and 0% for Control Group 6.

[0105] (5) Chlorine free radical (calculated as hypochlorite)

[0106] Under ultraviolet radiation, chlorides (such as chlorine gas and hypochlorous acid) can decompose to produce chlorine free radicals. Certain oxidants (such as ozone) can also react with chlorides to generate chlorine free radicals. Chlorine free radicals have strong oxidizing properties and can undergo addition reactions with biomolecules, leading to protein oxidation, lipid peroxidation, and DNA damage.

[0107] The chlorine free radical scavenging experimental method refers to CN108794656B.

[0108] Experimental results:

[0109] The superoxide anion radical scavenging rates of Examples 1 to 9 and Control Groups 1 to 6 are as follows: 81% for Example 1, 83% for Example 2, 82% for Example 3, 85% for Example 4, 76% for Example 5, 78% for Example 6, 80% for Example 7, 66% for Example 8, 80% for Example 9, 43% for Control Group 1, 47% for Control Group 2, 40% for Control Group 3, 38% for Control Group 4, 0% for Control Group 5, and 0% for Control Group 6.

[0110] (6) Nitrate radicals

[0111] Under ultraviolet radiation, nitrates can decompose to produce nitrate free radicals. Certain oxidants (such as ozone) can also react with nitrates to generate nitrate free radicals. Nitrate free radicals have strong oxidizing properties and can undergo addition reactions with biomolecules, leading to protein oxidation, lipid peroxidation, and DNA damage.

[0112] The experimental method of nitrate free radical scavenging was referred to “Li Genlin, Ma Yongjie, Sun Jingya, et al. Study on the scavenging effect of hemp seed sterol on DPPH* and nitrite free radicals [J]. Journal of Traditional Chinese Medicine, 2013, 28(5): 3. DOI: CNKI: SUN: HNZK.0.2013-05-034”.

[0113] Experimental results:

[0114] The superoxide anion radical scavenging rates of Examples 1 to 9 and Control Groups 1 to 6 are as follows: 81% for Example 1, 81% for Example 2, 76% for Example 3, 75% for Example 4, 80% for Example 5, 76% for Example 6, 78% for Example 7, 67% for Example 8, 79% for Example 9, 40% for Control Group 1, 56% for Control Group 2, 43% for Control Group 3, 42% for Control Group 4, 0% for Control Group 5, and 0% for Control Group 6.

[0115] The above results show that the antioxidants of various embodiments of the present invention have high scavenging capabilities for superoxide anion free radicals, hydroxyl free radicals, peroxynitrite anion free radicals, hydrogen peroxide free radicals, chlorine free radicals and nitrate free radicals, and have a wide range of applications.

[0116] 2. Antioxidant Experiment

[0117] The experimental method refers to "Wang Chunxia. Research on the regulation of blood lipid metabolism by copper-colored boletus polysaccharide [D]. Jilin Agricultural University, 2024", and the activity of superoxide dismutase and glutathione peroxidase was tested.

[0118] The results showed that compared with the control group 6, the superoxide dismutase activities of Examples 1 to 9 were increased by 35%, 36%, 33%, 35%, 33%, 33%, 34%, 29% and 35%.

[0119] Compared with the control group 2, the superoxide dismutase activities of Examples 1 to 9 were increased by 25%, 26%, 23%, 25%, 23%, 23%, 24%, 19% and 25%.

[0120] The results showed that compared with the control group 6, the activities of glutathione peroxidase in Examples 1 to 9 were increased by 43%, 45%, 43%, 44%, 40%, 43%, 43%, 37% and 44%.

[0121] Compared with the control group 2, the activities of glutathione peroxidase in Examples 1 to 9 can be increased by 22%, 23%, 21%, 20%, 21%, 22%, 20%, 16% and 23%.

[0122] These results demonstrate that the antioxidant preparation of the present invention can enhance the activities of superoxide dismutase and glutathione peroxidase. Furthermore, compared to control group 2, where no sodium chloride was added, the antioxidant preparation also promoted the enhanced activities of superoxide dismutase and glutathione peroxidase, indicating that sodium chloride, when combined with white peony root powder and bifidobacteria, can enhance their antioxidant properties.

[0123] It should be noted that when numerical ranges are involved in the present invention, it should be understood that both endpoints of each numerical range and any value between the two endpoints can be selected. Since the steps and methods used are the same as those in the embodiments, in order to avoid redundancy, the present invention describes preferred embodiments. Although preferred embodiments of the present invention have been described, those skilled in the art may make additional changes and modifications to these embodiments once they understand the basic inventive concept, and such changes and modifications fall within the scope of the present invention.

[0124] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. If these modifications and variations of the present invention fall within the scope of the equivalent technology of the present invention, the present invention is intended to include these modifications and variations.

Claims

1. An antioxidant preparation combining probiotics and Chinese medicinal materials, characterized in that: The mixture is prepared by allowing a mixture of white peony root powder, bifidobacteria and water to stand for 18 to 24 hours and then adding sodium chloride; the mass ratio of white peony root powder to water is 5 to 15:1000, and the concentration of bifidobacteria in the mixture is 10 7 The mass fraction of sodium chloride in the mixture is 0.8% to 0.9%.

2. The antioxidant preparation combining probiotics and Chinese medicinal materials according to claim 1, characterized in that: The bifidobacterium is Bifidobacterium adolescentis or Bifidobacterium infantis.

3. The antioxidant preparation combining probiotics and Chinese medicinal materials according to claim 1, characterized in that: The mass fraction of sodium chloride in the mixture is 0.85%.

4. The antioxidant preparation combining probiotics and Chinese medicinal materials according to claim 1, characterized in that: The concentration of live bifidobacteria in the mixed solution is 10 7 / mL~10 10 pieces / mL.

5. The method for preparing the antioxidant preparation combining probiotics and Chinese medicinal materials according to claim 1, characterized in that: include: preparing the white peony root into white peony root powder; The white peony root powder, bifidobacteria and water are mixed, left to stand for 18 to 24 hours, and sodium chloride is added to obtain a mixture which is an antioxidant preparation combining probiotics and Chinese medicinal materials; The mass ratio of white peony root and water is 5-15:1000, and the concentration of bifidobacteria in the mixture is 10 7 The mass fraction of sodium chloride in the mixture is 0.8% to 0.9%.

6. The method for preparing the antioxidant preparation combining probiotics and Chinese medicinal materials according to claim 5, characterized in that: The particle size of the white peony root powder is 20nm to 100nm.

7. The method for preparing the antioxidant preparation combining probiotics and Chinese medicinal materials according to claim 5, characterized in that: Adding auxiliary materials to the mixture to obtain a liquid preparation of an antioxidant preparation combining probiotics and Chinese medicinal materials.

8. The method for preparing the antioxidant preparation combining probiotics and Chinese medicinal materials according to claim 5, characterized in that: The auxiliary material includes at least one of an emulsifier, a dispersant, a thickener and a stabilizer.

9. The use of the antioxidant preparation combining probiotics and Chinese medicinal materials according to claim 1, characterized in that: The antioxidant preparation refers to a product that has the ability to scavenge free radicals and can enhance the activity of superoxide dismutase and glutathione peroxidase.

10. The use of the antioxidant preparation combining probiotics and Chinese medicinal materials according to claim 9, characterized in that: The free radicals include at least one of superoxide anion free radicals, hydroxyl free radicals, peroxynitrite anion free radicals, hydrogen peroxide free radicals, chlorine free radicals and nitrate free radicals.

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