48 results about "Fiber cell" patented technology

The application belongs to the field of functional material preparation, and particularly relates to a preparation method of a super-light high-nucleic-acid-absorption electrospun fiber sponge material and application thereof. The SiO2 electrospun fiber is used as a framework, a three-dimensional monolithic structure assembled by fragmented electrospun nanofiber membranes is used, the interconnected nanofiber cell structures of which endow the three-dimensional monolithic structure with high porosity and excellent structural flexibility and stability. Meanwhile, the amino-rich polyethylene imine and chitosan are chemically crosslinked by using epichlorohydrin as a crosslinking agent, and finally during freeze-drying, the ice crystals are partially sublimated, leaving a pore structure, and a porous sponge material is obtained. The material has a high nucleic acid adsorption capacity, and makes nucleic acid extraction more simple, rapid and sensitive.

The invention provides a low-GI biscuit raw material composition which comprises the following components in parts by weight: 0.1-0.6 part of nanocrystallized bamboo fiber cell walls, the nanocrystallized bamboo fiber cell walls are extracted from bamboo green, are edible and have one-dimensional characteristics, the transverse size is below 100nm, the longitudinal size is above 1mu m, cellulose in the nanocrystallized bamboo fiber cell walls has the crystallinity of 80%-95%, and the grain size of the cellulose in the nanocrystallized bamboo fiber cell walls is 100 nm. The dispersion liquid of the nanocrystallized bamboo fiber cell walls in water has the viscosity of 200 Pas or above when the mass concentration of the dispersion liquid is 0.5%, 40-70 parts of low-gluten flour, 20-35 parts of whole wheat flour, 8-20 parts of grease selected from natural butter, coconut oil or non-hydrogenated vegetable butter and 10-25 parts of sugar substitutes, and the sugar substitutes comprise at least one of erythritol and maltitol; the total mass content of the dietary fibers is greater than or equal to 15%. The beverage has a high low-GI effect, a suitable taste and good crush resistance. Methods of making low GI biscuits and low GI biscuits are also provided.

The invention discloses a method for extracting nanocrystallized bamboo fiber cell walls from bamboo green, which comprises the following steps: thermally activating a bamboo green material from natural bamboo wood in a weak base solution, and then carrying out organic acid mediated in-situ peroxy radical oxidation by using an organic acid-hydrogen peroxide mixture aqueous solution, then the water suspension is subjected to hydraulic separation under the hydraulic action to obtain bamboo fiber cells, and finally the obtained bamboo fiber cells are subjected to mechanical crushing and nanocrystallization refining treatment to obtain the needed nanocrystallization bamboo fiber cell walls. According to the method for extracting the nanocrystallized bamboo fiber cell wall, the natural moso bamboo is directly used as the raw material, the bamboo does not need to be pulped or smashed in advance, the preparation process is simple, the method is suitable for large-scale production, and the obtained nanocrystallized bamboo fiber cell wall product can be widely applied to the fields of food thickening, low-GI food additives and the like.

The invention belongs to the technical field of anti-adhesion materials in biomedical engineering, and relates to an anti-adhesion membrane with coarse structure fibers and a preparation method of the anti-adhesion membrane. The method comprises the following steps: soaking an electrostatic spinning fiber membrane containing a high polymer material and a hydrophilic anti-adhesion component in a treating fluid containing the high polymer material and consisting of a poor solvent and a good solvent for a specific time, rinsing and drying to obtain the anti-adhesion membrane, the swelling ratio of the high polymer material in the treating fluid at 25 DEG C for 10 minutes is 1-10% due to the proportion of the inferior solvent to the benign solvent in the treating fluid. A stable integrated nano shish-kebab structure is formed on the surface of the prepared anti-adhesion membrane fiber, exogenous drugs are not needed, spreading of fibroblasts is limited by means of a physical topological structure, conversion of the fibroblasts to myofibroblasts is blocked, the anti-adhesion effect is good, and biocompatibility is good.

The present application relates to the technical field of chemical fibers, and discloses a process for preparing lyocell pulp by mixing bamboo and wood pulp, comprising the following steps: bamboo pretreatment: cutting and shaping the bamboo, and then sequentially performing steam explosion defibration, acid treatment directional silicon removal and biological enzyme assisted delignification; wood pulp pretreatment: sequentially performing airflow sorting impurity removal, low-temperature alkali refining degreasing and biological enzyme deep purification; through the step-by-step directional pretreatment strategy, steam explosion causes microcracks in the cell wall of the short-cut bamboo fibers, and the high selectivity of the oxalic acid-ammonium fluoride composite acid liquid to silicate is used to achieve deep silicon removal of the short-cut bamboo fibers without damaging the cellulose skeleton; at the same time, the biological enzyme system step-by-step dissociates the hemicellulose side chain and the lignin aromatic ring structure, and removes the soluble oligomers, so that the risk of spinning blockage caused by silicon residues is eliminated under the premise of retaining the high polymerization degree cellulose of the bamboo, and the solubility of the pulp in the NMMO solvent is ensured.

This invention discloses the application of the cotton GhMYB transcription factor GhMYB4 gene in increasing cotton fiber length. This invention utilizes biotechnology to clarify that the GhMYB4 gene has a negative regulatory effect on fiber cell elongation, and downregulation of GhMYB4 gene expression leads to a significant increase in fiber length. Studies have shown that auxin content and auxin response pathways were significantly altered in GhMYB4-silenced cotton lines and transgenic Arabidopsis. In cotton fibers from GhMYB4-silenced lines after 20 days of development, Cers content was significantly increased. GhMYB4 inhibits the expression of the lipid transporter gene GhLTP4 by binding to the MYB cis-element on the GhLTP4 promoter. GhMYB4 affects fiber cell development by regulating auxin content, auxin response pathways, and lipid content. Regulating fiber cell elongation by inhibiting GhMYB4 expression has high breeding application value.

The invention discloses hydrogel capable of releasing growth factors after heat absorption and contraction and a preparation method of the hydrogel, and belongs to the field of biomedical materials. The hydrogel is of a three-layer composite structure, wherein the first layer is a sodium alginate-chitosan polyelectrolyte semi-interpenetrating network layer and serves as a compact protective layer; the second layer is a bacterial cellulose-sodium alginate high-toughness layer and is used as a bearing layer and a shrinkage driving layer; and the third layer is a basic fibroblast growth factor (bFGF)-loaded poly (butylene adipate / terephthalate) (PBAT) electrostatic spinning film layer and is used as a medicine storage cavern. According to the invention, the characteristic that the bacterial cellulose / sodium alginate layer can generate reversible heat absorption volume shrinkage at a specific temperature is used as a physical driving force to extrude and promote the bFGF in the third layer of PBAT fiber membrane to directionally release outwards.

The application discloses a self-powered electroactive dressing with a wound repair function and a preparation method and application thereof, and belongs to the technical field of medical devices. The electroactive dressing comprises a zinc-molybdenum primary battery structure and three layers of functionalized nanofiber scaffolds. The scaffold takes gelatin-polycaprolactone as a fiber matrix, sequentially loads zinc nanoparticles, icariin and molybdenum nanoparticles to form a functional layer, and is prepared through EDC / NHS cross-linking modification. The electroactive dressing has good mechanical properties and biocompatibility, can be minimally invasively implanted in a wound site, spontaneously generates a continuous and stable microelectric field through the zinc-molybdenum primary battery, and cooperatively realizes gradient-controlled release of icariin from burst release to sustained release, so that the effects of promoting fibroblast proliferation and migration and promoting rapid wound healing are achieved. The dressing has the advantages of simple preparation process, low cost, active regulation of a wound microenvironment, mild and controllable degradation, safety, no toxic side effects, and a very good application prospect.

The invention discloses application of a poplar PdPXC1 gene in regulating poplar growth, xylem development and cell wall component synthesis. A nucleotide sequence of a coding region of the PdPXC1 gene is shown as SEQ ID NO: 1, and an amino acid sequence coded by the PdPXC1 gene is shown as SEQ ID NO: 3. A PdPXC1 over-expressed or gene-edited transgenic poplar plant can be obtained by constructing a recombinant expression vector containing the gene or a CRISPR / Cas9 gene editing vector and transforming the poplar. Compared with a wild type, the plant height, the ground diameter, the cambium cell number, the xylem width, the cellulose content, the lignin content and the fiber cell wall thickness of the overexpressed plant are all remarkably increased; and the gene editing plant shows an opposite phenotype. The invention provides an important gene target and a breeding material for directionally improving the growth speed of the poplar and the wood quality through a genetic engineering means, and has a wide application prospect in forestry production.

The application belongs to the technical field of genetic engineering, and particularly relates to application of a poplar PtrCOB11 gene in increasing wood density. The application solves the problem of insufficient natural wood density and mechanical strength. The application relates to application of a recombinant vector or engineering bacteria of the poplar PtrCOB11 gene in increasing wood density. The application makes the stem segment xylem fiber cell wall S layer (secondary wall) thicken through overexpression of a PtrCOB11 gene strain, while not affecting the morphology of xylem cells. Overexpression of the PtrCOB11 gene of the application does not produce negative effects on the growth and development of plants. The application significantly promotes the development of the xylem fiber cell S layer of a poplar, and uniformly improves the density and mechanical strength of wood.

The purpose of the present invention is to provide a sea-island composite fiber in which the generation of an eluate caused by the decomposition of a functional group introduced into a polymer constituting a sea component is suppressed. Provided is a sea-island composite fiber having an island component and a sea component, the sea component being immobilized with a ligand and bonded by a covalent bond to a chlorine group-containing compound, the sea-island composite fiber satisfying formula 1, formula 1: 1.3 < = spectral intensity of 35Cl-in region A / spectral intensity of 35Cl-in region B < = 20.0, region A: region within 1.0 [mu] m from the outermost surface of the cross-section of the sea-island composite fiber, region B: spectral intensity of 35Cl-in region within 5.0 [mu] m from the center of gravity of the cross-section of the sea-island composite fiber: spectral intensity of 35Cl-in region within 5.0 [mu] m from the center of gravity of the cross-section of the sea-island composite fiber; and the spectral intensity of 35Cl-in the region when the spectral intensity of 25C2H measured by time-of-flight secondary ion mass spectrometry is 1.

The invention discloses application of a PdeAPL protein and a coding gene thereof in regulating and controlling properties of a poplar material. According to the invention, a transgenic poplar overexpressed with PdeAPL and a transgenic poplar with reduced PdeAPL gene expression quantity are constructed. Experiments prove that compared with a control group, the plant height, the number of stem nodes and the length of the stem nodes of the transgenic poplar subjected to overexpression of the PdeAPL are all remarkably increased, and leaves become smaller; and the plant height and the number of stem nodes of the transgenic poplar with reduced PdeAPL gene expression quantity are reduced, the length of the stem nodes is increased, and the leaves are enlarged. In addition, the xylem width of the transgenic poplar with reduced PdeAPL gene expression quantity is reduced, and the areas of xylem conduit cells, xylem fiber cells and phloem cells are increased. The method disclosed by the invention is of great significance to reveal the regulation and control molecular mechanism of the development of the stem vascular of the forest, the tree form of the forest, the growth speed and the improvement of the wood quality, and directionally cultivate the forest with the tree form or the material meeting the actual production requirements.

The invention discloses a wood fiber green dissociation method based on peroxide. The dissociation method comprises the following steps that a peroxide and a wood fiber raw material are stirred in a solvent, a dissociation product is obtained, and the peroxide is selected from sodium percarbonate. According to the method, partial lignin in an intercellular layer in a wood fiber cell wall of sodium percarbonate is used for transverse permeation and depolymerization, mechanical stirring is combined for longitudinal dissociation of the wood fiber cell wall, and a dissociation product with high lignin content and high crystallinity is prepared under the condition of low power consumption.

To provide a method for manufacturing a water body structure that promotes the formation of seaweed beds in water, which has a high effect in promoting seaweed bed formation and in which the sprayed layer is less likely to peel off from the substrate. [Solution] The method comprises a base layer formation step in which a base layer is formed by spraying a base layer spray material, which is a mixture of magnesium oxide, cotton-like bamboo fibers obtained by crushing and pulverizing raw bamboo rods so that the cell walls of the fiber cells are split, and granular anhydrous magnesium chloride with water or seawater, onto the surface of a substrate submerged in water; and a surface layer formation step in which, before the base layer formed in the base layer formation step solidifies, a surface layer spray material containing cotton-like bamboo fibers and granular sodium chloride is sprayed onto the surface of the base layer to form a surface layer in which the surface layer spray material has penetrated into the base layer.

The invention relates to the technical field of polypeptide compounds, in particular to a stapled peptide compound, a preparation method and application thereof and a skin care product. The structural formula of the stapled peptide compound is shown in the specification. The polypeptide has high anti-enzymolysis stability, can significantly improve the expression ability of up-regulated fiber cells and promote the expression of collagen, and can be used in the fields of wrinkle resistance, aging resistance, repair and the like.

The invention provides a coNOS gene and a gene engineering method thereof for improving cotton fiber traits. The invention discloses a new target, namely coNOS, which is a nitric oxide (NO) synthase, which can be used for improving fiber traits and / or yield traits of gossypium plants. Functional identification is carried out on the coNOS gene, and cotton plants with significantly increased fiber density, significantly increased fiber quantity and significantly increased yield are obtained through a transgenic technology. The invention also discloses the coNOS for increasing the content of nitric oxide in the fiber and controlling the synthesis of the cell wall of the fiber cell.

The invention provides rice noodles with low GI characteristic and a preparation method thereof, the rice noodles comprise rice flour, water and nano bamboo fiber cell walls, the nano bamboo fiber cell walls are extracted from bamboo green, are edible and have one-dimensional characteristics, the transverse size is below 100 nm, the longitudinal size is above 1 [mu] m, and the nano bamboo fiber cell walls are in one-dimensional characteristics. The mass content of the nano bamboo fiber cell walls in the rice noodles is between 0.1% and 1.5%. The invention also provides application of the nanocrystallized bamboo fiber cell wall as a low-GI rice noodle modifier and the low-GI rice noodle modifier.

The invention discloses a method for regulating and controlling mechanical strength of plant stalks and application of the method. By knocking out AXP1 and / or DARX1 in rice, the invention discovers that AXP1 and / or DARX1 mutation can lead to change of a stem xylan side chain structure, reduction of acetylation modification level on side chain arabinose, reduction of stem mechanical strength, thinning of cell walls of fiber cells and significant increase of Young modulus, which indicates that AXP1 and / or DARX1 mediate acetylation modification and glycosyl substitution of a side chain; and the method plays an important role in regulating and controlling the thickness of cell walls and mechanical properties of stalks. According to the invention, AXP1 and / or DARX1 mediated xylan side chain structure reconstruction is found for the first time, and the structure plays an important role as a key structure for docking of microcosmic force and macroscopic force. The invention provides a new gene resource and a new way for regulating and controlling the mechanical strength of the stalks through a side chain structure, and has a wide application prospect.

The invention discloses a gene GhCesA4D07 for regulating and controlling cotton fiber elongation and application thereof, the full-length sequence of the gene GhCesA4D07 is 3102bp, the gene GhCesA4D07 encodes a protein sequence with 1033 amino acids, and phenotypic investigation on virus-induced gene silencing and gene editing cotton shows that the fiber length and the fracture ratio strength are obviously shorter than those of a wild type. Therefore, the gene GhCesA4D07 plays an important role in regulating and controlling the elongation and growth of cotton fiber cells and the formation of related quality characters, and has a potential application value of improving the quality of cotton fibers.

The invention discloses a composite functional microsphere for repairing radiation damage and promoting collagen regeneration as well as a preparation method and application of the composite functional microsphere. The microsphere comprises a porous microsphere carrier prepared from a high polymer material with excellent biocompatibility and degradability, and repair polypeptide with functions of cell penetration, free radical removal, DNA protection and apoptosis resistance is loaded in the porous microsphere carrier or covalently bound on the surface of the porous microsphere carrier. The porous microsphere carrier disclosed by the invention is used as a long-term resident sustained-release storage cavern and a physical support bracket, can realize continuous and controllable release of polypeptide through internal pores or a surface modification layer of the microspheres, and can provide free radical scavenging and cell protection effects on an injured part for a long time; meanwhile, the stable three-dimensional space structure of the porous microsphere carrier can provide anchoring points for repairing cells such as fibroblasts and directly guide and support ordered deposition of new collagen fibers, so that substantial regeneration and reconstruction of a tissue structure are mechanically promoted while long-acting molecular protection is achieved.

The invention provides an application of a GhMAKR1 gene of upland cotton in regulation and control of cotton fiber elongation and plant morphology, 419 parts and 1081 parts of upland cotton varieties are re-sequenced and subjected to whole genome association analysis with fiber length traits, and it is found that the GhMAKR1 gene located in a D11 chromosome has significantly associated SNP and SV with the cotton fiber length. Gene overexpression and gene silencing means are used for verification and show that high expression of GhMAKR1 in a fiber development stage can significantly inhibit fiber elongation, and silencing causes fiber length increase. Meanwhile, research finds that the GhMAKR1 is used as an unknown functional protein, and overexpression of the GhMAKR1 shows a series of unique phenotypic characteristics, including dwarfing which is mainly shown in stem and internode shortening. In addition, overexpression plants also produce short and small flowers, the vigor of the flowers is reduced, and cotton bolls and seeds become small. The GhMAKR1 and the GhBIN2 protein can form an interaction compound to jointly inhibit the fiber cell elongation process.

The invention discloses application of a GhICR1 gene in adjusting the length of cotton fibers, and belongs to the technical field of gene engineering. According to the invention, the GhICR1 gene is excessively expressed in cotton, so that early elongation of cotton fiber cells is promoted, the length of mature fibers is increased, and the length of cotton fibers is increased. The cotton is improved by using the method, and a feasible strategy is provided for genetic improvement of the cotton fiber character length.

This invention belongs to the field of biomedical materials technology, specifically relating to a highly biocompatible and highly stable collagen fiber, its preparation method, and its application. The method includes the following steps: (1) constructing collagen fibers; (2) cross-linking collagen fibers with a cross-linking agent; (3) dialysis, homogenization, centrifugation, and collection of the precipitate. The prepared cross-linked collagen fibers are tightly packed and highly cross-linked, exhibiting good thermal stability and resistance to enzymatic degradation. The cross-linked collagen fibers have high biocompatibility, promoting fibroblast proliferation; they have high biocompatibility, and the inflammatory response caused after implantation is significantly mild and quickly eliminated. No calcification nodes appear after implantation of the cross-linked collagen fibers, demonstrating excellent anti-calcification effects. The cross-linked collagen fiber implant provided by this invention significantly promotes the production of new collagen and can be applied in fields such as implants, artificial skin, hemostatic sponges, scaffold materials, and medical devices, with broad application prospects.

This application relates to a dual-fiber battery position correction method and system. The method includes: rotating the battery in a preset direction; recording a first detection position when a first fiber pair detects the tab of the battery; continuing to rotate the battery in the preset direction; recording a second detection position when a second fiber pair detects the tab; determining the length of the movement path from the first detection position to the second detection position; obtaining a preset path range; and correcting the battery position based on either the first probe position or the second probe position when the movement path length is greater than or equal to the lower limit of the preset path range and less than the upper limit of the preset path range. This application avoids correction errors caused by tab bending and improves the accuracy of correction.

The invention provides a GSNOR gene and an application of the GSNOR gene in improved cotton plants. The GSNOR gene is subjected to isolation cloning and functional identification, and the expression quantity of the GSNOR gene is regulated and controlled through molecular biology and transgenic technologies, so that the regulation of plant growth and development is realized, and high-quality transgenic cotton with remarkably increased fiber quantity is obtained. The invention discloses functions and application of GSNOR protein and nitric oxide micromolecule signals, and particularly has positive effects in promoting fiber cell differentiation and improving cotton fiber density quality characters. The invention provides a new action target for variety improvement of cotton plants.

The invention discloses a plant fiber composite material cross-scale modeling simulation method based on a multi-level twisting structure, and the method comprises the steps: obtaining the elastic parameters of a fiber cell wall layer through three times of mixing rate compounding based on the mechanical properties of all components of plant fibers; a plant fiber multi-level structure model is constructed, and a stress-strain curve is obtained; establishing an equivalent fiber unit cell model, applying loads in six directions, and simulating nonlinear response; the method comprises the following steps: establishing a twisting RVE model of a plant fiber / epoxy composite material, introducing micromechanical parameters, applying a longitudinal tensile load, and simulating the macroscopic nonlinear response and untwisting time-varying behavior of the composite material. According to the method, the nonlinear deformation mechanism of the plant fiber composite material under different scales is effectively disclosed, and the problem that in the prior art, the complex multi-level structure and the dynamic deformation behavior of the plant fiber are understood insufficiently is solved.

The invention relates to the technical field of functional fertilizers, in particular to a high-yield fertilizer for coptis chinensis planting and a preparation method thereof.The high-yield fertilizer is prepared from, by weight, decomposed straw, sheep manure, urea, monopotassium phosphate, potassium sulfate, zinc sulfate, ferrous sulfate, manganese sulfate, modified humic acid and chitosan; after the thoroughly decomposed straws are fully decomposed, abundant organic matters can be provided, the air permeability and water retention of soil are improved, and a loose physical environment is created for the growth of the root system of coptis chinensis; high-pressure sterilization can thoroughly inactivate eggs and pathogenic bacteria remaining in the straw, and the risk of soil-borne diseases is avoided. Performance optimization of the modified sheep manure is realized through a three-step process of multi-stage low-temperature plasma treatment, composite enzymolysis and decomposition strengthening: in the first-stage plasma treatment, high-energy particles are utilized to destroy sheep manure fiber cell walls, so that a porous structure is formed, and the specific surface area is increased; the secondary treatment further oxidizes the surface of the fiber to generate active groups such as hydroxyl and carboxyl so as to provide action sites for subsequent enzymolysis.

The invention discloses application of GhHD-ZIP7 protein and a coding gene thereof in regulation and control of fiber length, and belongs to the technical field of plant genetic engineering. An overexpression vector is constructed for the GhHD-ZIP7 gene to transform arabidopsis thaliana and cotton, and it is found that the length of a main root of the transgenic arabidopsis thaliana is remarkably increased compared with that of a control (Columbia ecotype), the length of root hair in a mature region is remarkably shortened compared with that of the control, and the fiber length of an overexpression cotton material is remarkably reduced compared with that of a control (Zhong cotton No. 113). A knockout vector is constructed for the GhHD-ZIP7 gene to transform cotton, and it is found that the length of mature fibers of transgenic cotton with the GhHD-ZIP7 gene knocked out is remarkably increased compared with that of a control group. The research results show that the GhHD-ZIP7 gene regulates and controls the elongation of cotton fiber cells.

The application discloses a target object phenotype determination method based on a SOLOv2 model, which comprises the following steps: segmenting muscle fiber cells of a sample image to be determined by a weight-based target segmentation model to obtain a mask image and a first sample image; determining the total number of muscle fiber cells and the cell area of each muscle fiber cell according to muscle fiber cells in the first sample image, which have a cell overlapping area less than a first threshold value and a confidence degree greater than a second threshold value; classifying type I muscle cells and type II muscle cells by a dynamic relative color difference method according to the sample image to be determined to obtain the total number of type I muscle cells, the total number of type II muscle cells and the ratio of the two types of muscle fiber cells; determining the fat proportion of the sample image according to the mask image; and determining the phenotype of a target object according to the total number of muscle fiber cells, the ratio of the two types of muscle fiber cells and the fat proportion, so that the target image can be automatically segmented more quickly, the phenotype information of the target object in the image can be accurately obtained, and the method can be widely applied to the technical field of image processing.