79 results about "Fiber cell" patented technology

The application belongs to the field of functional material preparation, and particularly relates to a preparation method of a super-light high-nucleic-acid-absorption electrospun fiber sponge material and application thereof. The SiO2 electrospun fiber is used as a framework, a three-dimensional monolithic structure assembled by fragmented electrospun nanofiber membranes is used, the interconnected nanofiber cell structures of which endow the three-dimensional monolithic structure with high porosity and excellent structural flexibility and stability. Meanwhile, the amino-rich polyethylene imine and chitosan are chemically crosslinked by using epichlorohydrin as a crosslinking agent, and finally during freeze-drying, the ice crystals are partially sublimated, leaving a pore structure, and a porous sponge material is obtained. The material has a high nucleic acid adsorption capacity, and makes nucleic acid extraction more simple, rapid and sensitive.

The invention discloses application of a GhSP1L4D gene in regulation and control of cotton fiber elongation, and belongs to the technical field of gene engineering. The invention discovers that the GhSP1L4D gene presents specific high expression in the fiber development process for the first time, and especially the expression quantity is significant 20 days after flowering (20DPA). Furthermore, experiments prove that the GhSP1L4D gene can influence the helicity of cotton fibers, the thickness of cell walls of the cotton fibers and the content of cellulose and pectin in the cotton fibers so as to regulate and control the length of the cotton fibers, specifically, the expression of the GhSP1L4D gene is inhibited through virus induced gene silencing (VIGS) in the cotton; as a result, the fiber length is shortened, the cell length is shortened, the spiral number of the fiber is increased, the content of cellulose and pectin in the cotton fiber is increased, and new gene resources and theoretical support are provided for quality improvement of the cotton fiber.

The invention provides a low-GI biscuit raw material composition which comprises the following components in parts by weight: 0.1-0.6 part of nanocrystallized bamboo fiber cell walls, the nanocrystallized bamboo fiber cell walls are extracted from bamboo green, are edible and have one-dimensional characteristics, the transverse size is below 100nm, the longitudinal size is above 1mu m, cellulose in the nanocrystallized bamboo fiber cell walls has the crystallinity of 80%-95%, and the grain size of the cellulose in the nanocrystallized bamboo fiber cell walls is 100 nm. The dispersion liquid of the nanocrystallized bamboo fiber cell walls in water has the viscosity of 200 Pas or above when the mass concentration of the dispersion liquid is 0.5%, 40-70 parts of low-gluten flour, 20-35 parts of whole wheat flour, 8-20 parts of grease selected from natural butter, coconut oil or non-hydrogenated vegetable butter and 10-25 parts of sugar substitutes, and the sugar substitutes comprise at least one of erythritol and maltitol; the total mass content of the dietary fibers is greater than or equal to 15%. The beverage has a high low-GI effect, a suitable taste and good crush resistance. Methods of making low GI biscuits and low GI biscuits are also provided.

The invention discloses a method for extracting nanocrystallized bamboo fiber cell walls from bamboo green, which comprises the following steps: thermally activating a bamboo green material from natural bamboo wood in a weak base solution, and then carrying out organic acid mediated in-situ peroxy radical oxidation by using an organic acid-hydrogen peroxide mixture aqueous solution, then the water suspension is subjected to hydraulic separation under the hydraulic action to obtain bamboo fiber cells, and finally the obtained bamboo fiber cells are subjected to mechanical crushing and nanocrystallization refining treatment to obtain the needed nanocrystallization bamboo fiber cell walls. According to the method for extracting the nanocrystallized bamboo fiber cell wall, the natural moso bamboo is directly used as the raw material, the bamboo does not need to be pulped or smashed in advance, the preparation process is simple, the method is suitable for large-scale production, and the obtained nanocrystallized bamboo fiber cell wall product can be widely applied to the fields of food thickening, low-GI food additives and the like.

The utility model belongs to the technical field of dressings, and discloses a micro-current foam application which comprises a protective layer, a dressing layer is arranged on the bottom side of the protective layer, a piezoelectric layer is arranged on the bottom side of a bonding layer, and a release layer is arranged on the bottom side of the piezoelectric layer. When the piezoelectric material is in contact with the skin, the surface potential of the piezoelectric material is changed or the piezoelectric material is subjected to external pressure, micro-current can be automatically generated, pressure is generated on the piezoelectric layer when the dressing is pasted on the skin so as to generate the micro-current, or when the piezoelectric material is in contact with the wound, the surface potential is changed so as to generate the micro-current, and an endogenous electric field at the wound is recovered. Therefore, proliferation and migration of nerve cells and fiber cells are promoted, the wound healing speed is increased, the piezoelectric layer releases current, an endogenous electric field at the wound is recovered, wound healing is promoted, the dressing layer absorbs percolate on the surface of the wound, and skin around the wound is prevented from being soaked.

A nanodiamond-reinforced nanofiber scaffold system for skin regeneration, consisting of a multilayered, electrospun nanofiber matrix formed from one or more biocompatible polymers selected from polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), chitosan, gelatin or collagen, wherein nanodiamonds are present in an amount of 0.1 to 5 wt.-% are incorporated and surface-functionalized with oxygen, hydroxyl, or carboxyl groups to ensure homogeneous dispersion within the polymer matrix, the scaffold being characterized by a controlled fiber diameter in the range of 100 to 800 nm, an interconnected porosity between 60 and 90%, a tensile strength of 2 to 10 MPa, a degradation time of 2 to 8 weeks, and the ability to improve cell adhesion, proliferation, and migration of fibroblasts and keratinocytes, while simultaneously exhibiting antioxidant activity, reduced formation of reactive oxygen species, and the optional incorporation of bioactive agents selected from growth factors, antioxidants, or antimicrobial compounds to accelerate wound healing and skin tissue regeneration.

The present invention relates to thermoset biopolymers derived from isolated keratin fiber cell components, to composite keratin materials comprising thermoset biopolymers, to processes for their preparation and to their uses.

The invention belongs to the technical field of anti-adhesion materials in biomedical engineering, and relates to an anti-adhesion membrane with coarse structure fibers and a preparation method of the anti-adhesion membrane. The method comprises the following steps: soaking an electrostatic spinning fiber membrane containing a high polymer material and a hydrophilic anti-adhesion component in a treating fluid containing the high polymer material and consisting of a poor solvent and a good solvent for a specific time, rinsing and drying to obtain the anti-adhesion membrane, the swelling ratio of the high polymer material in the treating fluid at 25 DEG C for 10 minutes is 1-10% due to the proportion of the inferior solvent to the benign solvent in the treating fluid. A stable integrated nano shish-kebab structure is formed on the surface of the prepared anti-adhesion membrane fiber, exogenous drugs are not needed, spreading of fibroblasts is limited by means of a physical topological structure, conversion of the fibroblasts to myofibroblasts is blocked, the anti-adhesion effect is good, and biocompatibility is good.

The present application relates to the technical field of chemical fibers, and discloses a process for preparing lyocell pulp by mixing bamboo and wood pulp, comprising the following steps: bamboo pretreatment: cutting and shaping the bamboo, and then sequentially performing steam explosion defibration, acid treatment directional silicon removal and biological enzyme assisted delignification; wood pulp pretreatment: sequentially performing airflow sorting impurity removal, low-temperature alkali refining degreasing and biological enzyme deep purification; through the step-by-step directional pretreatment strategy, steam explosion causes microcracks in the cell wall of the short-cut bamboo fibers, and the high selectivity of the oxalic acid-ammonium fluoride composite acid liquid to silicate is used to achieve deep silicon removal of the short-cut bamboo fibers without damaging the cellulose skeleton; at the same time, the biological enzyme system step-by-step dissociates the hemicellulose side chain and the lignin aromatic ring structure, and removes the soluble oligomers, so that the risk of spinning blockage caused by silicon residues is eliminated under the premise of retaining the high polymerization degree cellulose of the bamboo, and the solubility of the pulp in the NMMO solvent is ensured.

The invention provides a GhSBP1 gene for regulating cotton fiber development, a regulation method and breeding application, and belongs to the technical field of gene engineering. The invention provides a GhSBP1 gene for regulating and controlling the development of cotton fibers. The CDS sequence of the GhSBP1 gene comprises a sequence as shown in SEQ ID No. 1. According to verification in the embodiment of the invention, the GhSBP1 gene is over-expressed in cotton, the fiber length of the over-expressed GhSBP1 cotton in different periods after flowering is longer than that of a wild type cotton, and the over-expressed GhSBP1 can significantly increase the fiber length and the number of initial fiber cells, which indicates that the GhSBP1 gene regulates and controls the fiber length of the fiber cells. The GhSBP1 gene is transferred into cotton, so that the elongation of cotton fiber cells can be promoted, the cell wall thickness of the cotton fiber cells can be increased, and the GhSBP1 gene has great significance in research and improvement of cotton fiber quality.

The invention discloses a double-bionic wound dressing with seepage management and self-power generation functions as well as a preparation method and application of the double-bionic wound dressing. The double-bionic wound dressing comprises a first electrode layer, a blocking layer, a moisture absorption power generation layer, a diffusion antibacterial layer, an evaporation power generation layer and a second electrode layer which are sequentially stacked from bottom to top. The electrostatic spinning fiber membrane with high biocompatibility is adopted, a liquid transport mechanism of plant transpiration and an ion active transport principle of electric ray power generation cells are combined through bionic design, the triple functions of wound surface antibiosis, efficient seepage management and stable power generation are achieved, the wound surface microenvironment is regulated and controlled, and the wound surface antibacterial effect is achieved. Excessive inflammation caused by seepage accumulation is effectively avoided, fibroblast migration, angiogenesis and skin appendage regeneration are remarkably promoted, wound healing is accelerated, skin function recovery is facilitated, and a new treatment mode is provided for burn wound repair.

This invention discloses the application of the cotton GhMYB transcription factor GhMYB4 gene in increasing cotton fiber length. This invention utilizes biotechnology to clarify that the GhMYB4 gene has a negative regulatory effect on fiber cell elongation, and downregulation of GhMYB4 gene expression leads to a significant increase in fiber length. Studies have shown that auxin content and auxin response pathways were significantly altered in GhMYB4-silenced cotton lines and transgenic Arabidopsis. In cotton fibers from GhMYB4-silenced lines after 20 days of development, Cers content was significantly increased. GhMYB4 inhibits the expression of the lipid transporter gene GhLTP4 by binding to the MYB cis-element on the GhLTP4 promoter. GhMYB4 affects fiber cell development by regulating auxin content, auxin response pathways, and lipid content. Regulating fiber cell elongation by inhibiting GhMYB4 expression has high breeding application value.

The invention discloses hydrogel capable of releasing growth factors after heat absorption and contraction and a preparation method of the hydrogel, and belongs to the field of biomedical materials. The hydrogel is of a three-layer composite structure, wherein the first layer is a sodium alginate-chitosan polyelectrolyte semi-interpenetrating network layer and serves as a compact protective layer; the second layer is a bacterial cellulose-sodium alginate high-toughness layer and is used as a bearing layer and a shrinkage driving layer; and the third layer is a basic fibroblast growth factor (bFGF)-loaded poly (butylene adipate / terephthalate) (PBAT) electrostatic spinning film layer and is used as a medicine storage cavern. According to the invention, the characteristic that the bacterial cellulose / sodium alginate layer can generate reversible heat absorption volume shrinkage at a specific temperature is used as a physical driving force to extrude and promote the bFGF in the third layer of PBAT fiber membrane to directionally release outwards.

The application discloses a self-powered electroactive dressing with a wound repair function and a preparation method and application thereof, and belongs to the technical field of medical devices. The electroactive dressing comprises a zinc-molybdenum primary battery structure and three layers of functionalized nanofiber scaffolds. The scaffold takes gelatin-polycaprolactone as a fiber matrix, sequentially loads zinc nanoparticles, icariin and molybdenum nanoparticles to form a functional layer, and is prepared through EDC / NHS cross-linking modification. The electroactive dressing has good mechanical properties and biocompatibility, can be minimally invasively implanted in a wound site, spontaneously generates a continuous and stable microelectric field through the zinc-molybdenum primary battery, and cooperatively realizes gradient-controlled release of icariin from burst release to sustained release, so that the effects of promoting fibroblast proliferation and migration and promoting rapid wound healing are achieved. The dressing has the advantages of simple preparation process, low cost, active regulation of a wound microenvironment, mild and controllable degradation, safety, no toxic side effects, and a very good application prospect.

The invention relates to the technical field of chemical fibers, and discloses a process for preparing lyocell pulp by mixing bamboo wood and wood cotton and carrying out directional treatment, which comprises the following steps: bamboo wood pretreatment: sequentially carrying out steam explosion splitting, acid treatment directional silicon removal and biological enzyme assisted delignification on the cut and formed bamboo wood; the raw material wood cotton is sequentially subjected to airflow separation and impurity removal, low-temperature alkali refining and degreasing and biological enzyme deep purification; through a step-by-step directional pretreatment strategy, micro cracks are generated on cell walls of the chopped bamboo fibers through steam explosion, and deep silicon removal of the chopped bamboo fibers is realized without damaging a cellulose skeleton in cooperation with the high-selectivity dissolving capacity of oxalic acid-ammonium fluoride composite acid liquor to silicate; meanwhile, a hemicellulose side chain and a lignin aromatic ring structure are dissociated step by step by a biological enzyme system, and soluble oligomers are removed, so that the spinning blockage risk caused by silicon residues is eliminated on the premise that high-polymerization-degree cellulose of bamboo wood is reserved, and the dissolution smoothness of pulp in an NMMO solvent is guaranteed.

The invention discloses application of a poplar PdPXC1 gene in regulating poplar growth, xylem development and cell wall component synthesis. A nucleotide sequence of a coding region of the PdPXC1 gene is shown as SEQ ID NO: 1, and an amino acid sequence coded by the PdPXC1 gene is shown as SEQ ID NO: 3. A PdPXC1 over-expressed or gene-edited transgenic poplar plant can be obtained by constructing a recombinant expression vector containing the gene or a CRISPR / Cas9 gene editing vector and transforming the poplar. Compared with a wild type, the plant height, the ground diameter, the cambium cell number, the xylem width, the cellulose content, the lignin content and the fiber cell wall thickness of the overexpressed plant are all remarkably increased; and the gene editing plant shows an opposite phenotype. The invention provides an important gene target and a breeding material for directionally improving the growth speed of the poplar and the wood quality through a genetic engineering means, and has a wide application prospect in forestry production.

The application discloses a kind of fire-resistant impregnated plant fiber laminated decorative board preparation method, it is related to fire-resistant decorative board preparation technical field, for solving the problem that traditional market decorative fireproof plate exists poor permeability, poor weather resistance, easy to deform, short service life;The present application is selected low density, high water absorption plant fiber density board, by immersing phenolic resin glue, through heating, roll and cooling cooling process obtains a kind of fireproof, moisture-proof, oil-proof, anti-static, good weather resistance and corrosion-resistant not easy to deform a fireproof plate, small molecule high permeability resin is preferentially impregnated and filled into wood fiber cell wall and cell cavity crosslinking, then impregnated or coated macromolecular flexible resin improves flexibility, realizes the toughening of impregnated wood fiber;At the same time, various decorative papers can be rolled on the surface of the fireproof plate during rolling, with integrated characteristics, so the plant fiber decorative board is more flexible, more resistant to impact and more durable.

The application belongs to the technical field of genetic engineering, and particularly relates to application of a poplar PtrCOB11 gene in increasing wood density. The application solves the problem of insufficient natural wood density and mechanical strength. The application relates to application of a recombinant vector or engineering bacteria of the poplar PtrCOB11 gene in increasing wood density. The application makes the stem segment xylem fiber cell wall S layer (secondary wall) thicken through overexpression of a PtrCOB11 gene strain, while not affecting the morphology of xylem cells. Overexpression of the PtrCOB11 gene of the application does not produce negative effects on the growth and development of plants. The application significantly promotes the development of the xylem fiber cell S layer of a poplar, and uniformly improves the density and mechanical strength of wood.

The invention belongs to the technical field of plant expression vectors and application thereof, and discloses a method for improving fiber length by changing extracellular body acidification degree, which comprises the following steps: fusing a specific promoter with a PM < H + >-ATP enzyme gene to construct a plant expression vector for specifically expressing PM < H + >-ATP enzyme, and integrating the plant expression vector into a cotton genome to obtain the fiber length. According to the method, the pH value of fiber cytoplasm exosome is moderately reduced in the fiber elongation process, the length of the cotton fiber can be remarkably increased, and high-quality fiber is provided for the cotton industry and the textile industry. According to the method for improving the length of the cotton fiber, AHA related genes are specifically expressed in fiber cells of cotton, then the acidification degree of cell walls in the fiber elongation process is regulated and controlled, the cell walls are moderately acidified and relaxed, development of the cotton fiber is controlled, and the purpose of increasing the length of the cotton fiber is achieved. Test results prove that the length of the cotton fibers improved by the method for improving the characters of the cotton fibers is obviously increased.

The purpose of the present invention is to provide a sea-island composite fiber in which the generation of an eluate caused by the decomposition of a functional group introduced into a polymer constituting a sea component is suppressed. Provided is a sea-island composite fiber having an island component and a sea component, the sea component being immobilized with a ligand and bonded by a covalent bond to a chlorine group-containing compound, the sea-island composite fiber satisfying formula 1, formula 1: 1.3 < = spectral intensity of 35Cl-in region A / spectral intensity of 35Cl-in region B < = 20.0, region A: region within 1.0 [mu] m from the outermost surface of the cross-section of the sea-island composite fiber, region B: spectral intensity of 35Cl-in region within 5.0 [mu] m from the center of gravity of the cross-section of the sea-island composite fiber: spectral intensity of 35Cl-in region within 5.0 [mu] m from the center of gravity of the cross-section of the sea-island composite fiber; and the spectral intensity of 35Cl-in the region when the spectral intensity of 25C2H measured by time-of-flight secondary ion mass spectrometry is 1.

The application discloses a GHCDC48 gene and application thereof in improving fiber strength of Gossypium hirsutum. The gene GHCDC48 is from Gossypium hirsutum, and a nucleotide sequence in a genetic standard strain TM-1 of Gossypium hirsutum is shown as SEQ ID NO. 1. The gene expression level is positively correlated with the fiber strength of cotton. Overexpression of the GHCDC48 gene in Gossypium hirsutum can significantly improve the ATP enzyme activity in cotton fiber cells, increase the secondary wall thickness of the fiber and the fiber strength. Decreasing the expression of the GHCDC48 gene in Gossypium hirsutum reduces the growth rate of the Gossypium hirsutum plants, the ATP enzyme activity in the cells and the secondary wall thickness. Therefore, the GHCDC48 gene can be used to improve the fiber strength of Gossypium hirsutum. The research of the application lays a foundation for establishing new germplasm of Gossypium hirsutum with high fiber strength, and has important practical significance for basic scientific research or future agricultural industry application.

The present invention discloses a composite multi-layer anti-adhesion membrane and a preparation method thereof. Carboxymethyl cellulose and a biodegradable high molecular polymer are made into a composite membrane. The surface carboxymethyl cellulose contacts the damaged tissue to provide initial adhesion so that the membrane is tightly attached to the tissue, avoiding movement of the isolation membrane and leakage of the anastomosis. It can effectively stop bleeding and prevent other body fluids and fibrinogen from seeping out. The gradual dissolution of the carboxymethyl cellulose in the mixed fiber layer avoids the carboxymethyl cellulose from being diluted too quickly and absorbed by the body, making it difficult to maintain a stable concentration and amount at the damaged site for a long time. As the carboxymethyl cellulose in the mixed fiber layer is gradually diluted and absorbed, the remaining loose high molecular polymer structure can guide fibroblasts to grow into the membrane, promote wound healing, and keep the anti-adhesion membrane fixed to the tissue. The high molecular polymer base layer on the other side has a dense structure that can effectively prevent fibroblasts from breaking through the anti-adhesion membrane, providing an anti-adhesion effect.

The invention discloses application of a PdeAPL protein and a coding gene thereof in regulating and controlling properties of a poplar material. According to the invention, a transgenic poplar overexpressed with PdeAPL and a transgenic poplar with reduced PdeAPL gene expression quantity are constructed. Experiments prove that compared with a control group, the plant height, the number of stem nodes and the length of the stem nodes of the transgenic poplar subjected to overexpression of the PdeAPL are all remarkably increased, and leaves become smaller; and the plant height and the number of stem nodes of the transgenic poplar with reduced PdeAPL gene expression quantity are reduced, the length of the stem nodes is increased, and the leaves are enlarged. In addition, the xylem width of the transgenic poplar with reduced PdeAPL gene expression quantity is reduced, and the areas of xylem conduit cells, xylem fiber cells and phloem cells are increased. The method disclosed by the invention is of great significance to reveal the regulation and control molecular mechanism of the development of the stem vascular of the forest, the tree form of the forest, the growth speed and the improvement of the wood quality, and directionally cultivate the forest with the tree form or the material meeting the actual production requirements.

The invention discloses a wood fiber green dissociation method based on peroxide. The dissociation method comprises the following steps that a peroxide and a wood fiber raw material are stirred in a solvent, a dissociation product is obtained, and the peroxide is selected from sodium percarbonate. According to the method, partial lignin in an intercellular layer in a wood fiber cell wall of sodium percarbonate is used for transverse permeation and depolymerization, mechanical stirring is combined for longitudinal dissociation of the wood fiber cell wall, and a dissociation product with high lignin content and high crystallinity is prepared under the condition of low power consumption.

To provide a method for manufacturing a water body structure that promotes the formation of seaweed beds in water, which has a high effect in promoting seaweed bed formation and in which the sprayed layer is less likely to peel off from the substrate. [Solution] The method comprises a base layer formation step in which a base layer is formed by spraying a base layer spray material, which is a mixture of magnesium oxide, cotton-like bamboo fibers obtained by crushing and pulverizing raw bamboo rods so that the cell walls of the fiber cells are split, and granular anhydrous magnesium chloride with water or seawater, onto the surface of a substrate submerged in water; and a surface layer formation step in which, before the base layer formed in the base layer formation step solidifies, a surface layer spray material containing cotton-like bamboo fibers and granular sodium chloride is sprayed onto the surface of the base layer to form a surface layer in which the surface layer spray material has penetrated into the base layer.

The application discloses a growth factor-loaded electrospun dressing, characterized by comprising the following components in percentage by mass: 55-85% of synthetic polymer, 9.5-36.4% of chitosan, 3.3-13.5% of bovine serum albumin, 0.07-0.14% of sodium triphosphate, 0.004-0.063% of basic fibroblast growth factor, and the rest being water, wherein the total of the percentage by mass of the above components is 100%. The growth factor-loaded electrospun dressing solves the problems of poor cell affinity and lack of cell climbing sites of smooth electrospun fiber dressing.

The invention relates to the technical field of polypeptide compounds, in particular to a stapled peptide compound, a preparation method and application thereof and a skin care product. The structural formula of the stapled peptide compound is shown in the specification. The polypeptide has high anti-enzymolysis stability, can significantly improve the expression ability of up-regulated fiber cells and promote the expression of collagen, and can be used in the fields of wrinkle resistance, aging resistance, repair and the like.

The invention relates to the technical field of polypeptide compounds, in particular to a stapled peptide compound, a preparation method and application thereof and a skin care product. The structural formula of the stapled peptide compound is shown in the specification. The polypeptide has high anti-hydrolysis stability, can significantly improve the expression ability of up-regulated fiber cells and promote the expression of collagen, and can be used in the fields of wrinkle resistance, aging resistance, repair and the like.

The invention relates to the technical field of grass raw material processing and utilization, in particular to a vascular bundle plant fiber and parenchyma separation method. According to the method for separating the vascular bundle tissue and the parenchyma tissue, the bamboo wood is pretreated through the low-concentration alkali method, the bagasse is sampled in batches through the density method, the bamboo wood can be treated under the mild condition, the vascular bundle tissue and the parenchyma tissue can be effectively separated, and the parenchyma tissue in the slurry state can be obtained in the separation process through mild alkali treatment. The bagasse raw material is sampled in batches by adopting a density method which is easy to operate to obtain vascular bundle tissues and parenchyma tissues which are rich in fibrocytes and parenchyma cells, and the cell structures are complete and are not damaged. The yields of vascular bundle tissues and parenchyma tissues of bamboo wood are 65.39% and 9.76% respectively, and the yields of vascular bundle tissues and parenchyma tissues of bagasse are 16.44% and 27.67% respectively. The vascular bundle tissues of the bamboo wood and the bagasse separated by the method can be used for preparing high-performance paper pulp through a subsequent alkaline cooking process, and have the advantages of high cellulose content, good drainability, low inorganic matter content and the like.