93results about How to "Avoid loss of activity" patented technology

The invention is directed to pharmaceutical compositions useful in the treatment of migraine. The compositions contain metoclopramide and one or more NSAIDs in unit dosage form. By selecting NSAIDs that are non-acidic or segregating the metoclopramide and NSAID, the storage life of the compositions has been increased. Also disclosed are coordinated dosage forms for the sequential release of drugs. The invention encompasses methods of treating migraine using any of these dosage forms.

The present invention encompasses a solid dose delivery vehicle for ballistic administration of a bioactive material to subcutaneous and intradermal tissue, the delivery vehicle being sized and shaped for penetrating the epidermis. The delivery vehicle further comprises a stabilizing polyol glass loaded with the bioactive material and capable of releasing the bioactive material in situ. The present invention further includes methods of making and using the solid dose delivery vehicle of the invention.

The invention discloses a chromatographic medium using amino benzimidazole as a function ligand and a preparation method thereof. Hydrophilic porous microspheres are used as a chromatographic medium, activated by allyl bromide, and coupled with the amino benzimidazole to obtain a medium using the amino benzimidazole as the function ligand; dimethyl sulfoxide and the allyl bromide are sequentially added into a chromatographic matrix for activation; the activated chromatographic matrix is reacted with N-bromo-succinimide for bromo-alcoholization; the bromo-alcoholized chromatographic matrix is mixed with an amino benzimidazole solution for coupling the amino benzimidazole ligand; finally an aqueous ethanol amine solution is used for sealing unreacted bromo-alcoholized ends to obtain a hydrophobic charge induced chromatographic medium using the amino benzimidazole as the function group. The new chromatographic medium is simple in preparation process and high in antibody adsorption capacity, and has the characteristics of non salt dependent adsorption, can realize desorption and recovery by changing the solution pH to weak acid, and can be used for hydrophobic charge induction chromatographic separation of antibodies.

The invention discloses a method for obtaining transgenic bovine fetal fibroblast by using Cas9 cutting nuclease-mediated Ipr1 fixed point insertion. The bovine fetal fibroblast is cotransfected by adonor vector and a CRISPR / Cas0 expression vector for a target locus through an electroporation method, an MSR1 promoter in the donor vector can realize specific expression in full-time phagocytic cells, and after puromycin drug screening, the targeted positive clone cell is obtained through PCR authentication. The transgenic positive clone cell is used as a donor cell so as to obtain a transgenicclone embryo, the transgenic clone embryo is further transplanted into the uterus of a rutting acceptor calf, and finally a living Ipr1 fixed point insertion transgenic cloned calf is obtained.

The invention discloses a method for preparing phycocyanin from fresh spirulina. The process design is based on convenience, high efficiency and capability of facilitating comprehensive utilization of different components, the fresh spirulina is used as a processing raw material, and the spirulina phycocyanin is prepared through steps of low-temperature wall breakage, ammonium sulfate fractional salting-out, polyethylene glycol 2000 / (NH4)2SO4 two-aqueous-phase extraction, ultrafiltration and vacuum freezing drying. With the adoption of the method, the yield of the spirulina phycocyanin is higher, the obtained spirulina phycocyanin is low in polysaccharide content, the purity A620 / A280 is about 3.7, the method is applicable to preparation of the phycocyanin from other algae used as raw materials, the biological activity of all components of the spirulina can be well kept during preparation, other obtained separated parts can be further processed, a series of products such as active polysaccharides, chlorophyll, algal protein and the like can be obtained, and the method has the high promotional value and the broad application prospect.

The invention relates to a curcumin or curcumin-ramification-containing composite feed additive and a use thereof. The use of the additive can promote the growth of the animals, and / or protect the livers of the animals, and / or improve the oxidation resistance of the animals, reduce the generation of lipid peroxide, and improve the immunity and the disease resistance, and / or improve the stress resistance of the fish, avoid the problem of 'irritable haemorrhage', promote the secretion of fish mucus, and solve the problems that the cultivated fish is easy to loosen squama, easy to fall off, and lack of fish mucus. The invention further relates to a method for cultivating the animals and an animal feed which contains the composite feed additive having the curcumin or curcumin ramification.

The invention belongs to the field of pharmaceutical preparations, and relates to preparation of a novel target nano carrier and application of the novel target nano carrier in a camptothecin drug targeting delivery system. The system can be represented as PAMAM-HA / drug, wherein PAMAM is polyamide amine-amine arboraceous macromolecules of different generations, HA is hyaluronic acid of different molecular weights, and the drug is one or more of camptothecin anticancer drugs such as camptothecin, 10- hydroxycamptothecine, topotecan and the like. The carrier is used for wrapping the camptothecin drugs, and has the actions of prolonging the drug blood circulation time, improving the drug bioavailability, reducing the non-tumor part concentration of the drug, reducing the drug cytotoxicity, and simultaneously protecting the lactonic rings of the camptothecin drugs from damage, thereby improving the tumor treatment efficiency from multiple aspects. The nano carrier provided by the invention has important significance in promoting the safe delivery of the camptothecin drugs and the effective treatment of tumor.

The invention discloses a slow sinking type puffing aquatic product matched pellet feed. The pellet feed contains 9-15% of carbohydrate and 8-22% of grease. The puffing degree of the pellet feed is 1.1-1.25, and the volume mass is 380-420kg / m<3>. The pellet feed is a special feed with low sugar and high grease for artificial carnivorous fish culture. The invention also provides producing equipment and method of the pellet feed. The producing equipment comprises a crusher, a mixer, a quality controlling machine, a dual-screw puffing machine, a granulating machine, a dryer and the like which are sequentially connected. A screw assembly in the dual-screw puffing machine consists of a delivering area screw, a kneading area screw and a forming area screw. The invention can obviously enhance the quality of the fish, greatly reduce the generation of fatty liver in the fishes, and ensure the uniform size of the finished fishes.

The invention provides an exenatide slow-release microsphere composition prepared from an exenatide drug and polymers.The exenatide accounts for 2 %w / w-15 %w / w of the total weight of the microsphere, the polymers are selected from one or more of polycaprolactone, polylactic acid, a polylactic acid-hydroxyacetic acid copolymer and polyanhydride, the particle size of the microsphere ranges from 20 micrometers to 90 micrometers, and the relative biological activity retention rate of the exenatide is higher than 90%.The invention further provides a preparation method of the microsphere composition.According to the preparation method, the problem that the activity losses of the drug on an oil-water interface are caused due to usage of a water phase can be completely avoided on the condition that a protective agent does not need to be added, and the encapsulation rate and the biological activity retention rate of the exenatide are increased; homogeneous turbid liquid or a homogenous solution of submicron drug particles is formed through the solubility differences of the drug in different solvents, micronized drug particles do not need to be prepared, therefore, the preparation technology is simplified, and the burst release effect is avoided.

The invention discloses an aluminum-plated metal carrier dehydrogenation catalyst and a preparation method thereof, which belong to the field of catalyst applications. The catalyst consist of a metal carrier layer, a gamma-Al2O3 porous material layer and a catalyst layer, wherein the metal carrier layer is made of an aluminum-plated steel plate or aluminum-plated alloy; and the catalyst layer consists of a precious metal and / or aid with catalytic activity. The preparation method comprises the following steps of: cleaning and drying the metal carrier layer; completely converting an aluminum-plated layer into Al2O3 through anodic oxidation; corroding and broaching; coating or dipping a layer of active Al2O3 on the surface of the broached Al2O3; drying and sintering to obtain a gamma-Al2O3 porous material layer; coating or dipping a catalyst layer; and drying, baking and reducing to obtain the aluminum-plated metal carrier dehydrogenation catalyst. In the catalyst, the bonding strength between the catalyst layer and the metal carrier is high, and the activity of the catalyst layer is high; and the preparation method is simple, and is low in cost.

The present invention is related to a pharmaceutical composition suitable for the treatment and / or the prevention of atherosclerosis from infectious origin, which comprises an adequate pharmaceutical carrier, a corticosteroid and a stilbene-type alexin, preferably further comprising a flavonoid to regenerate the stilbene and / or to increase the effect of the latter. The latter compositions are highly suitable for long-term therapies like the treatment of atherosclerosis from infectious origin.

The invention belongs to the technical field of food, and particularly relates to a probiotics powder and a preparation method thereof. The probiotics powder of the invention comprises the following components in parts by weight: 8-16 parts of Pu'er tea enzyme powder, 10-18 parts of multiple fungus powder, 6-8 parts of soybean protein isolate powder, 6-12 parts of spirulina powder, 12-20 parts ofmodified yeast glucan, 2-6 parts of black bamboo salt, 4-8 parts of honey and 32-46 parts of fructo-oligosaccharide. The probiotics powder provided by the invention is reasonable and scientific in compatibility, and the components interact with each other. The probiotics powder not only has a good taste and good stability, but also has the effects of moistening intestines, relaxing bowels, expelling toxins, lowering fat and reducing weight.

The invention relates to a porous spherical core-shell structure silicon-carbon composite material and a preparation method thereof and a lithium ion battery. The method comprises the following stepsof: preparing a mixed solution of Si / SiO2 nanoparticles by S1; S2 preparing a mixed solution 2 of polymer 1 and polymer 2; S3 spray precursor spherical Si / SiO2 / polymer composites; SiO2 / C composites with porous spherical core-shell structure are prepared by sintering Si / SiO2 / C composites and then etching SiO2 layer with strong acid to remove SiO2 layer. In the invention, two polymers with differentthermal decomposition temperatures are firstly heated in air to burn off the polymer with low thermal decomposition temperature to form nano pores, and then high-temperature carbonization is carriedout in an inert gas, which is different from the pores formed by natural carbonization using a single carbon source. The silicon-carbon composite material obtained by the invention is a multi-core shell structure spherical agglomerate, and has obvious nano holes on the surface, small specific surface area and high vibration density. The charge-discharge capacity and cycle performance of the lithium ion battery using the silicon-carbon composite material are obviously improved.

The present invention relates to immobilization of fusion proteins to surfaces. According to this invention a fusion polypeptide comprises an adhesion polypeptide fused to a preselected polypeptide. Specifically the method takes advantage of the spontaneous immobilization properties of the adhesion polypeptide part of the fusion protein.

The invention discloses blueberry fruit gelatin filled candies. The blueberry fruit gelatin filled candies comprise candy coatings and candy fillers wrapped with the candy coatings, wherein the candyfillers comprise xylitol, sorbitol and a blueberry fruit anthocyanin extract; and the candy coatings comprise one or more of granulated sugar, high fructose corn syrup, blueberry fruit juice, raspberry juice, sorbitol, mannitol, xylitol, isomaltitol and galactitol which are compounded, and comprise gelatin, pectin, VC, vitamin B1 and vitamin D3. According to the filled candies disclosed by the invention, the new mouth feel and the new flavor of the filled candies are increased, and the filled candies disclosed by the invention have the efficacy of neutralizing the effect of alcoholic drinks, protecting the liver, resisting cancer and reducing weight. In the preparation process of the candies, a superhigh pressure low-temperature sterilizing manner is adopted, so that the loss of active substances of blueberry fruit anthocyanin, raspberry flavone and the like caused by temperature rising, is reduced; and besides, the pectin and anthocyanin molecules are combined, so that the anthocyaninstability is improved. The filled candies disclosed by the invention have important significance in enriching of slurry fruit product kinds, enlarging the industry chain of slurry fruit processing, increasing the added value of slurry fruits, and promoting industry development.

The invention relates to an endometrium stem cell serum-free medium, which is prepared from the following components in percentage by total volume: 70 to 85 percent of low-sugar DMEM (Dulbecco Modified Eagle Medium), 5 to 10 percent of serum replacement, 2 to 10 percent of vitamin C, 1 to 5 percent of L-glutamine, 1.25 to 15 percent of glucose, 1 to 10 percent of 2-mercaptoethanol, 0.8 to 1.5 percent of mycillin double-antibody solution, and 0.8 to 1.5 percent of gentamicin sulphate. The endometrium stem cell serum-free medium provided by the invention is adopted for carrying out cell culture, and has the characteristics of less medium component, stable endometrium stem cell growth, high multiplication capacity and high purity.

The invention belongs to the technical field of biomedicines and particularly relates to a composition for improving premature ovarian failure, a preparation method therefor and application of the composition. According to the composition for improving the premature ovarian failure, provided by the invention, an in-vitro-constructed human uterine endometrium stem-cell cell membrane and a mesenchymal stem cell exosome are used in a compounded manner, thus, the development of ovarian follicles can be promoted, ovaries can be promoted to secrete an anti-Mullerian hormone, an ovarian follicle generator promoting level can be regulated, the apoptosis of ovarian cells can be inhibited, the adverse reserve function disease of the ovaries can be improved, the effect of improving primary or secondary premature ovarian failure is good, and thus, a new way of think is provided for clinically treating ovarian insufficiency and polycystic ovarian syndrome.

The invention discloses an application of Aristolochia fordiana volatile oil in preparation of cancer preventing and treating and / or antibacterial products. Extraction of the Aristolochia fordiana volatile oil includes steps of: 1) pulverizing Aristolochia fordiana and adding water with stirring, performing water vapor distillation extraction to prepare a water system containing volatile oil; 2) adding an organic solvent to the water system containing volatile oil for extraction, abandoning separated water layer, adding a dehydration drying agent to the organic solvent for dehydration, and filtering and recovering the organic solvent to obtain dried pure volatile oil. The Aristolochia fordiana extract, obtained through the water vapor distillation process, has excellent antibacterial and anti-cancer activity.

The invention discloses a chromatographic medium and a preparation method thereof. The chromatographic medium comprises a chromatographic substrate and a functional ligand; the chromatographic substrate is agarose gel microspheres; the functional ligand is coupled N-benzyl-N-methylethanolamine activated via allyl glycidyl ether. The preparation method includes the steps of 1) activating the chromatographic substrate with allyl glycidyl ether to obtain an activated chromatographic substrate; 2) brominating the activated chromatographic substrate of step 1) via bromine water to form a brominatedchromatographic substrate; 3) subjecting the brominated chromatographic substrate of step 2) to reaction with the functional ligand N-benzyl-N-methylethanolamine to form the chromatographic medium. The preparation process of the chromatographic medium is simple; antibody adsorbing capacity is high; exfoliated protein A can be well removed by changing pH at the premise of achieving antibody adsorbing and desorbing, the protein A can be used to capture and purify the medium, and two-step purification of an antibody is achieved.

The invention discloses a chromatographic medium using amino benzimidazole as a function ligand and a preparation method thereof. Hydrophilic porous microspheres are used as a chromatographic medium, activated by allyl bromide, and coupled with the amino benzimidazole to obtain a medium using the amino benzimidazole as the function ligand; dimethyl sulfoxide and the allyl bromide are sequentially added into a chromatographic matrix for activation; the activated chromatographic matrix is reacted with N-bromo-succinimide for bromo-alcoholization; the bromo-alcoholized chromatographic matrix is mixed with an amino benzimidazole solution for coupling the amino benzimidazole ligand; finally an aqueous ethanol amine solution is used for sealing unreacted bromo-alcoholized ends to obtain a hydrophobic charge induced chromatographic medium using the amino benzimidazole as the function group. The new chromatographic medium is simple in preparation process and high in antibody adsorption capacity, and has the characteristics of non salt dependent adsorption, can realize desorption and recovery by changing the solution pH to weak acid, and can be used for hydrophobic charge induction chromatographic separation of antibodies.

The invention discloses a preparation method of a human blood coagulation factor VIII and a human blood coagulation factor VIII product and relates to the field of blood products. The preparation method of the human blood coagulation factor VIII comprises the following steps: dissolving a cryoprecipitate with a 0.015-0.025mol / L tromethamine solution in a mass ratio of (3-5): 1 by reasonably processing the cryoprecipitate; and carrying out separation and purification by means of a polyethylene glycol precipitating method combined with ion exchange chromatography, wherein the recovery ratio of the human blood coagulation factor VIII and the specific activity of a final product can be improved effectively, the yield reaches 180-240IU / L plasma, and the specific activity is not lower than 100IU / mg proteins. The prepared human blood coagulation factor VIII product is rich in vWF factors and the proportion of the vWF factors and the human blood coagulation factor VIII is close to 1: 1. Besides treating hemophiliac A, the human blood coagulation factor VIII can be also used for treating patients with angiohemophilia, and the human blood coagulation factor VIII has good stability and heat resistance.

The invention provides a restriction endonuclease DpnI preparation and a preparation method thereof. The restriction endonuclease preparation is prepared through an in-vitro protein synthesis reaction, specifically, an in-vitro acellular protein synthesis system is constructed, a DNA template containing a coding gene of DpnI is added, the in-vitro protein synthesis reaction is carried out, a target protein with a DpnI structure is synthesized, solid-liquid separation is carried out, supernatant is collected, the enzyme activity is measured, a supernatant solution with restriction endonucleaseactivity is selected, the restriction endonuclease DpnI preparation is obtained, and the preparation is packaged or is prepared into a storage solution and then packaged. According to the product, a preparation method is simple, the protein content loss caused by traditional separation and purification steps are avoided, the enzyme digestion activity is high, and the preparation can be widely applied to the fields of molecular biology, bioengineering, food and the like.

The invention discloses a preparation method of a liquid fresh dendrobium officinale kimura ex migo beverage. The preparation method comprises the following steps of (1) cleaning the fresh dendrobium officinale kimura ex migo, and cutting into sections; (2) putting the dendrobium officinale kimura ex migo sections into a low-temperature freezing crushing machine, and crushing, so as to obtain super-fine powder of dendrobium officinale kimura ex migo; (3) extracting the super-fine powder of dendrobium officinale kimura ex migo for two times at room temperature, filtering by a sterile screen plate, and combining the two times of filtrates, so as to obtain fresh dendrobium officinale kimura ex migo extracting liquid; (4) adding 0.01% to 0.05% of vitamin C, 0.05% to 0.1% of preservative, 0.01% to 0.1% of stabilizer and 4% to 6% of honey into the extracting liquid, and uniformly mixing; (5) subpackaging, and sterilizing for at least 24h at the temperature of -80 to -90 DEG C. The invention also discloses the liquid dendrobium officinale kimura ex migo beverage. The liquid dendrobium officinale kimura ex migo beverage has the advantages that the content of effective ingredients is high, the absorbing is easy, the flavor is good, the property is stable, the warranty period is long, and the like.

The invention discloses a dehydrogenation catalyst taking a Fe-Cr-Al alloy as a metal carrier and a preparation method thereof, which belong to the field of catalysts. The catalyst consists of a metal carrier layer, a gamma-Al2O3 porous material layer and a catalyst layer, wherein the metal carrier layer is made of a Fe-Cr-Al alloy; and the catalyst layer consists of a precious metal and / or aid with catalytic activity. The preparation method comprises the following steps of: calcining the Fe-Cr-Al alloy to till A1 in the alloy leaks out and is oxidized into Al2O3; corroding and broaching; coating or dipping a layer of active Al2O3 on the surface of the broached Al2O3; drying and sintering to obtain a gamma-Al2O3 porous material layer; coating or dipping a catalyst layer; and drying, baking and reducing to obtain the catalyst. In the catalyst, the bonding strength between the catalyst layer and the metal carrier is high, and the activity of the catalyst layer is high; and the preparation method is simple, and is low in cost.

The present invention relates to dry delivery system comprising a xerogel or film with applied active ingredients for topical active ingredient delivery or other purposes. Said delivery system are obtainable by a method according to the invention. The present invention also provides for methods for achieving defined localization of stable or unstable active substances on dry xerogels or films, which can be reconstituted into hydrogels. From the obtained delivery systems, the active substances are released with advantageous release kinetics.

The invention discloses a method for extracting, purifying and detecting anthriscus nemorosa volatile oil and application of the method. According to the method, the anthriscus nemorosa volatile oil is obtained through extracting and purifying by virtue of water steam distillation and organic extraction techniques for the first time, the extracting-purifying method is simple in process and low in cost, distilled water and an organic solvent in a preparation process can be repeatedly utilized, and the volatile oil prepared by virtue of the preparation process is low in moisture content; and meanwhile, experiments prove that the volatile oil has good oxidation resistance, antibacterial activity and anticancer activity, has good antibacterial effects on staphylococcus aureus, bacillus subtilis, escherichia coli and pseudomonas aeruginosa, has good anticancer and cancer-inhibiting effects on human hepatoma carcinoma cells and human breast cancer cells and is applicable to industrial production and application.