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Method for preparing phycocyanin from fresh spirulina

A technology of phycocyanin and spirulina, which is applied in the direction of chemical instruments and methods, decapeptides, peptides, etc., can solve the problems of high-purity phycocyanin being expensive, restricting the application of phycocyanin, and low comprehensive utilization rate, so as to alleviate the The effect of reducing pressure, reducing production costs, and saving energy

Inactive Publication Date: 2015-06-03
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no clear and reliable phycocyanin deep-processing product on the market. This is because most of the existing reports use processes such as salting out, centrifugation, dialysis and column chromatography to separate and purify algae from spirulina and other algae raw materials. cyanocyanin, but in the actual production process, there are disadvantages such as cumbersome steps, high energy consumption, low yield, long operation cycle, and low comprehensive utilization rate, which lead to the extremely expensive price of high-purity phycocyanin, which restricts the production of phycocyanin. application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for preparing phycocyanin with fresh spirulina, which specifically comprises the following steps:

[0028] 1) Freeze 500g of fresh spirulina at -20°C for 24 hours, thaw, pretreat with a colloid mill, homogeneously break the wall under a pressure of 25MPa, centrifuge at 1300Orpm for 15min, remove the precipitate, and obtain a centrifugal liquid;

[0029] 2) Take the centrifuged liquid obtained in step 1), slowly add powdered solid ammonium sulfate under rapid stirring, so that the saturation of ammonium sulfate in the mixed liquid reaches 25%, let it stand for 2 hours, and centrifuge at 1300Orpm for 15 minutes to remove the precipitate and obtain a second centrifugation separating liquid;

[0030] 3) Take the centrifuged liquid obtained in step 2), slowly add powdered solid ammonium sulfate under rapid stirring, so that the saturation of ammonium sulfate in the mixed liquid reaches 55%, let it stand for 2 hours, centrifuge at 1300Orpm for 15min, remove the centr...

Embodiment 2

[0035] A method for preparing phycocyanin with fresh spirulina, which specifically comprises the following steps:

[0036] 1) Freeze 1000g of fresh spirulina at -20°C for 8h, thaw, pretreat with colloid mill, homogeneously break the wall under 30MPa pressure, centrifuge at 1300Orpm for 15min, remove the precipitate, and obtain a centrifuge liquid;

[0037]2) Take the centrifuged liquid obtained in step 1), slowly add powdered solid ammonium sulfate under rapid stirring, so that the saturation of ammonium sulfate in the mixed liquid reaches 25%, let it stand for 2 hours, and centrifuge at 1300Orpm for 20min to remove the precipitate and obtain a second centrifugation separating liquid;

[0038] 3) Take the centrifuged liquid obtained in step 2), slowly add powdered solid ammonium sulfate under rapid stirring, so that the saturation of ammonium sulfate in the mixed liquid reaches 55%, let it stand for 2 hours, centrifuge at 1300Orpm for 15min, remove the centrifuged liquid, and ...

Embodiment 3

[0043] A method for preparing phycocyanin with fresh spirulina, which specifically comprises the following steps:

[0044] 1) Freeze 2000g of fresh spirulina at -20°C for 4h, thaw, pretreat with a colloid mill, homogeneously break the wall under a pressure of 35MPa, centrifuge at 1300Orpm for 15min, remove the precipitate, and obtain a centrifuge;

[0045] 2) Take the centrifuged liquid obtained in step 1), slowly add powdered solid ammonium sulfate under rapid stirring, so that the saturation of ammonium sulfate in the mixed liquid reaches 25%, let it stand for 2 hours, and centrifuge at 1300Orpm for 25min to remove the precipitate and obtain the second centrifugation separating liquid;

[0046] 3) Take the centrifuged liquid obtained in step 2), slowly add powdered solid ammonium sulfate under rapid stirring, so that the saturation of ammonium sulfate in the mixed liquid reaches 55%, let it stand for 2 hours, centrifuge at 1300Orpm for 15min, remove the centrifuged liquid, a...

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Abstract

The invention discloses a method for preparing phycocyanin from fresh spirulina. The process design is based on convenience, high efficiency and capability of facilitating comprehensive utilization of different components, the fresh spirulina is used as a processing raw material, and the spirulina phycocyanin is prepared through steps of low-temperature wall breakage, ammonium sulfate fractional salting-out, polyethylene glycol 2000 / (NH4)2SO4 two-aqueous-phase extraction, ultrafiltration and vacuum freezing drying. With the adoption of the method, the yield of the spirulina phycocyanin is higher, the obtained spirulina phycocyanin is low in polysaccharide content, the purity A620 / A280 is about 3.7, the method is applicable to preparation of the phycocyanin from other algae used as raw materials, the biological activity of all components of the spirulina can be well kept during preparation, other obtained separated parts can be further processed, a series of products such as active polysaccharides, chlorophyll, algal protein and the like can be obtained, and the method has the high promotional value and the broad application prospect.

Description

technical field [0001] The invention belongs to the technical field of deep processing of functional components of algae, and in particular relates to a method for efficiently preparing phycocyanin with fresh spirulina. Background technique [0002] At present, there are many varieties of spirulina tablets, capsules, and compound beverages produced on the market based on spirulina powder. However, due to the lack of a quality evaluation system in the industry, some manufacturers ignore the quality control during the production process, resulting in similar The production process and product quality of the products are quite different. At the same time, the exaggerated publicity of the efficacy of the products has caused people to doubt the nutrition, safety, and efficacy of spirulina products, which has seriously affected the development and application of spirulina products and the healthy development of the spirulina industry. . [0003] In order to ensure the sustainable...

Claims

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Application Information

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IPC IPC(8): C07K14/795C07K1/36C07K1/34C07K1/30
CPCC07K14/795
Inventor 叶舟齐清华陈宇熹涂秋金张月珠雷绍南吴晓芹罗永亮
Owner FUJIAN AGRI & FORESTRY UNIV
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