Application of Aristolochia fordiana volatile oil in preparation of cancer preventing and treating and/or antibacterial products

A technology of volatile oil and Tongchenghu, applied in the application field of Tongchenghu volatile oil in the preparation of cancer prevention and/or antibacterial products, can solve the problems of less Tongchenghu and other problems, and achieve low moisture content, good bacteriostasis and anti-cancer Active, heat-sensitive avoidance effect

Inactive Publication Date: 2018-10-23
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few studies on Tongcheng tiger, and most of them are taken orally and decocted, or smashed or ground for external application; there are no reports on the extraction and use of the volatile oil of Tongcheng tiger

Method used

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  • Application of Aristolochia fordiana volatile oil in preparation of cancer preventing and treating and/or antibacterial products
  • Application of Aristolochia fordiana volatile oil in preparation of cancer preventing and treating and/or antibacterial products
  • Application of Aristolochia fordiana volatile oil in preparation of cancer preventing and treating and/or antibacterial products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment 1: Pre-experiment

[0066] Different extraction methods were used to extract the volatile oil of Tongcheng Tiger, as follows:

[0067] 1. Steam distillation method:

[0068] (1) Take 500g of dried Tongchenghu powder, add 1500g of distilled water and stir to carry out steam distillation. The distillation extraction time is 5 hours to obtain a water system containing volatile oil;

[0069] (2) Add ether to the water system containing volatile oil for extraction, discard the separated water layer, add anhydrous sodium sulfate to the ether solution containing volatile oil for dehydration, filter and recover ether to obtain dry and pure volatile oil.

[0070] 2. Simultaneous distillation extraction method:

[0071] Weigh 500g of dried Tongchenghu powder into a distillation flask towel, add 1500g of distilled water, and at the same time measure ether into the distillation flask, connect the simultaneous distillation and extraction device, and distill and extract ...

Embodiment 2

[0078] Embodiment 2: the extraction and purification method of Tongcheng tiger volatile oil

[0079] (1) Take 500g of fresh Tongcheng tiger, wash and dry it, and then powder it to a particle size of 60 mesh, add 1500g of distilled water, stir and carry out steam distillation, and the distillation extraction time is 5h to obtain a water system containing volatile oil;

[0080] (2) Add diethyl ether to the water system containing volatile oil for extraction, discard the separated water layer, add anhydrous sodium sulfate to the diethyl ether solution containing volatile oil for dehydration, filter and recover diethyl ether to obtain dry and pure volatile oil; the extraction rate is 0.32% , the water content in the volatile oil is 0.55%.

Embodiment 3

[0081] Embodiment 3: the detection method of Tongcheng tiger volatile oil

[0082] Adopt GC-MS to detect the Tongchenghu volatile oil that embodiment 2 obtains, the condition of concrete detection is as follows: HP-5MS chromatographic column (30m * 0.25mm * 0.25 μ m); Carrier gas: helium; Flow rate: 1.2mL / min , the solvent is delayed for 5 minutes; heating program: the initial temperature is 60°C, keep for 1min, raise the temperature to 200°C at 6°C / min, keep for 5min; raise the temperature to 280°C at 5°C / min, keep for 2min. Ionization method: EI, 70eV; ion source temperature: 250°C; carrier gas: helium; column flow rate: 1.2mL / min. A total of 51 chemical components were detected in the volatile oil of Tongcheng tiger. The specific chemical components and relative contents are shown in Table 2.

[0083] Table 2 Chemical composition of volatile oil of Tongcheng tiger

[0084]

[0085]

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Abstract

The invention discloses an application of Aristolochia fordiana volatile oil in preparation of cancer preventing and treating and/or antibacterial products. Extraction of the Aristolochia fordiana volatile oil includes steps of: 1) pulverizing Aristolochia fordiana and adding water with stirring, performing water vapor distillation extraction to prepare a water system containing volatile oil; 2) adding an organic solvent to the water system containing volatile oil for extraction, abandoning separated water layer, adding a dehydration drying agent to the organic solvent for dehydration, and filtering and recovering the organic solvent to obtain dried pure volatile oil. The Aristolochia fordiana extract, obtained through the water vapor distillation process, has excellent antibacterial and anti-cancer activity.

Description

technical field [0001] The invention relates to the application of Tongchenghu volatile oil in the preparation of cancer prevention and / or antibacterial products. Background technique [0002] Volatile oils, also known as essential oils, are a general term for a class of oily liquids that are volatile at room temperature, distillable with water vapor, and immiscible with water. Most volatile oils have an aromatic odor. Volatile oil is an important active ingredient. In addition to the direct application of crude drugs mainly containing volatile oil, the volatile oil refined therefrom, such as eucalyptus oil and peppermint oil, can also be used clinically. Volatile oil has the effects of dispersing and detoxifying the exterior, rejuvenating the body with aroma, regulating qi and relieving pain, expelling wind and dampness, promoting blood circulation and removing blood stasis, dispelling cold and warming the interior, clearing heat and detoxification, relieving heat and elim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B9/02A23L33/105A61K36/264A61P31/04A61P35/00
CPCA23V2002/00A61K36/264A61K2236/37A23L33/105A61P31/04A61P35/00C11B9/022C11B9/025C11B9/027A23V2200/308
Inventor 赖鹏翔高阳
Owner SHANDONG UNIV
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