Method for extracting, purifying and detecting anthriscus nemorosa volatile oil and application of method

A technology for the purification method of A. chinensis and its purification method, which is applied in the fields of anti-oxidation, discovery and identification of volatile oil components of A. spp. , There are no problems such as the volatile oil of ginseng ginseng, which achieves the effect of low water content, avoiding quality reduction, and improving extraction rate

Inactive Publication Date: 2017-08-04
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the prior art has researches on the components of Asparagus ginseng, there is no report about the volatile oil of Ashen chinensis in the prior art

Method used

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  • Method for extracting, purifying and detecting anthriscus nemorosa volatile oil and application of method
  • Method for extracting, purifying and detecting anthriscus nemorosa volatile oil and application of method
  • Method for extracting, purifying and detecting anthriscus nemorosa volatile oil and application of method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] The method for extracting and purifying the volatile oil of E. japonicus of embodiment 1

[0084] (1) Take 500g of fresh ginseng, wash and dry it, and then powder it to a particle size of 60 mesh, add 1000g of distilled water, stir and carry out steam distillation, and the extraction time of distillation is 4 hours to obtain a water system containing volatile oil;

[0085] (2) Add ether to the water system containing volatile oil for extraction, discard the separated water layer, add anhydrous sodium sulfate to the ether solution containing volatile oil for dehydration, filter and recover ether to obtain dry and pure volatile oil.

Embodiment 2

[0086] The detection method of the volatile oil of embodiment 2 spinosa ginseng

[0087] Adopt GC-MS to detect the volatile oil of the spinosa ginseng that embodiment 1 obtains, concrete detection condition is as follows: HP-5MS chromatographic column (30m * 0.25mm * 0.25 μ m); Carrier gas: helium; Flow velocity: 1.2mL / min , the solvent is delayed for 4 minutes; heating program: the initial temperature is 60°C, hold for 1 minute, raise the temperature to 200°C at 15°C / min, hold for 5 minutes, then rise to 280°C at 5°C / min, and hold for 2 minutes. Ionization method: EI, 70eV; ion source temperature: 250°C; carrier gas: helium; column flow rate: 1.2mL / min. A total of 45 chemical components were detected in the volatile oil of E. japonicus. The specific chemical components and their relative contents are shown in Table 2.

[0088] Table 2 The chemical composition of the volatile oil of A. japonicus

[0089]

[0090]

Embodiment 3

[0091] Example 3 Anti-oxidation test of the volatile oil of E. japonicus

[0092] 1. Preparation of DPPH test solution Preparation of 0.08mmol / L DPPH solution: Precisely weigh 8.0mg of DPPH, dissolve it with absolute ethanol and set the volume to a 200ml brown volumetric flask to obtain a DPPH solution with a concentration of 0.004%, and store it away from light ,spare.

[0093] 2. Experimental steps: Take 1.0ml of each sample solution (0.24, 0.48, 0.72, 0.96, 1.20mg / ml) of different concentrations, put it into a 10ml centrifuge tube, add 3.0ml of DPPH solution, and react at room temperature for 30min in the dark. At the same time, absolute ethanol was used as a blank, and the absorbance was measured at a wavelength of 517 nm. Calculate the DPPH free radical scavenging rate according to the following formula.

[0094] DPPH free radical scavenging rate (%)=A0-(As-Ac) / A0×100%

[0095] In the formula, A0—absorbance value of 1.0ml distilled water+3.0ml DPPH solution, As—absorba...

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Abstract

The invention discloses a method for extracting, purifying and detecting anthriscus nemorosa volatile oil and application of the method. According to the method, the anthriscus nemorosa volatile oil is obtained through extracting and purifying by virtue of water steam distillation and organic extraction techniques for the first time, the extracting-purifying method is simple in process and low in cost, distilled water and an organic solvent in a preparation process can be repeatedly utilized, and the volatile oil prepared by virtue of the preparation process is low in moisture content; and meanwhile, experiments prove that the volatile oil has good oxidation resistance, antibacterial activity and anticancer activity, has good antibacterial effects on staphylococcus aureus, bacillus subtilis, escherichia coli and pseudomonas aeruginosa, has good anticancer and cancer-inhibiting effects on human hepatoma carcinoma cells and human breast cancer cells and is applicable to industrial production and application.

Description

technical field [0001] The invention belongs to the technical field of research and development of pharmaceutical products, in particular to a method for extracting, purifying, and detecting volatile oil of A. Antioxidant, antibacterial and anticancer functions. Background technique [0002] Volatile oils, also known as essential oils, are a general term for a class of oily liquids that are volatile at room temperature, distillable with water vapor, and immiscible with water. Most volatile oils have an aromatic odor. Volatile oil is an important active ingredient. In addition to the direct application of crude drugs mainly containing volatile oil, the volatile oil refined therefrom, such as eucalyptus oil and peppermint oil, can also be used clinically. Volatile oil has the effects of dispersing and dispelling the exterior, rejuvenating the fragrance, regulating qi and relieving pain, expelling wind and dampness, promoting blood circulation and removing blood stasis, dispe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/14G01N30/02
CPCG01N30/02G01N30/06G01N30/14G01N2030/062
Inventor 赖鹏翔张瀚予陶委琦高阳
Owner SHANDONG UNIV
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