78results about How to "Loss of activity" patented technology

The present invention provides antimicrobial coatings for coating substrate surfaces, particularly medical devices, for preventing bacterial adhesion and biofilm formation by inhibiting microbial growth and proliferation on the coating surface. The antimicrobial coatings are composed of a hydrogel and a bioactive agent including a substantially water-insoluble antimicrobial metallic material that is solubilized within the coating. Antimicrobial coating formulations for obtaining such coatings, and coating methods are also described.

A method of preparing a crimped tow of cellulose acetate filaments comprising the steps of: a) providing cellulose acetate dope b) forming filaments (23) from the dope c) applying at least one additive to the filaments d) crimping the filaments to form a crimped tow wherein the at least one additive is capable of removing a component from cigarette smoke. Preferably, the component is a Hoffmann analyte. The additive may comprise a solution, liquid, emulsion or particulate material or combinations thereof. Preferably, the additive comprises an acidic compound or an alkaline compound. The additive may comprise malic acid, potassium carbonate, citric acid, tartaric acid, lactic acid, ascorbic acid, polyethyleneimine, cyclodextrin, sodium hydroxide, sulphamic acid, sodium sulphamate, polyvinyl acetate and carboxylated acrylate, carbon, silica, zeolite, clay, alumina, metal, molecular sieves or an ion exchange resin. The product tow can be processed on standard equipment to make efficient filter rods from which cigarette filter tips can be made which give significantly increased and selective retention of key smoke constituents.

A new class of 2,2':6',2''-terpyridine-platinum (II) and substituted 2,2':6',2''-terpyridine-platinum (II) complexes in which an N- or O- or halo nucleophile is the fourth ligand to platinum. The compounds are potent intercalators of DNA. Some have antitumour activity. Some have anti-parasitic activity. A new method of preparing the complexes involves reacting a Pt complex of 1,5-cyclooctadiene with a 2,2':6',2''-terpyridine.

The present invention discloses cosmetic or topical pharmaceutical compositions for body slimming, firming, cellulite reduction, fat-reduction, and obesity control benefits that can be selective and specific for external body parts and organs such as face, chin, cheeks, arms, "love handles" in abdomen area, eye lids and eye zone, neck, breasts, thighs, and hips. These compositions include a synergistic, bioavailability-enhanced ion-pair combination of Hydroxycitric acid or Hydroxycitric acid derivatives with certain organic bases such as Niacinamide, Niacin, Pyridoxine, Aminophylline, Caffeine, Carnitine, Creatine, Chitosan, Allantoin, Glucosamine, Phaseolamine, Chromium Picolinate, Theobromine, Theophylline, and such.

Doxorubicin block copolymer formulations for use in preparing injectable compositions for treating cancer patients which contain lactose for solubilizing the doxorubicin and block copolymers in said formulations and methyl paraben for stabilizing these formulations, as well as a method of preparing and using these injectable compositions.

The invention relates to a preparation method of addition type liquid fluorinated silicone rubber. D3F, ViD4 and MM with the purity larger than 99.5% are adopted as raw materials and evenly mixed according to a certain ratio, and vinyl fluorosilicone oil is obtained; the vinyl fluorosilicone oil is used as base gum, a reinforcement agent, a cross-linking agent and a catalyst are added in the vinyl fluorosilicone oil, kneading, grinding, tabletting and other technologies are performed, and the liquid fluorinated silicone rubber (FSR) is obtained; the insulation performance, hydrophobic property, the high-temperature resistance and the like of the LSR are better than that of liquid silicone rubber (LSR) with the same technology and formula. The preparation method is short in production period, the product has the good heat tear resistance, demolding is convenient, various machining ways of mold pressing, extrusion, injection molding and the like can be achieved, and automatic production materials can be achieved.

The invention discloses a seaweed liquid fertilizer with a drought resisting function and a preparation method and application of the seaweed liquid fertilizer. The seaweed liquid fertilizer comprisesthe following components: a seaweed drought resisting functional carrier, external drought resisting substances, humic acid, chemical fertilizers and a surfactant, wherein the seaweed drought resisting functional carrier is prepared by implementing activity maintenance treatment on an extract prepared from fresh seaweeds through enzymolysis and mycogenic composition in sequence, implementing molecular membrane grading separation and by implementing drought resisting activity detection screening on crop seedlings. The preparation method comprises the following steps: uniformly mixing the seaweed drought resisting functional carrier and the external drought resisting substance, compounding with the humic acid and the chemical fertilizers in sequence, and finally adding the surfactant and the like, thereby obtaining the seaweed liquid fertilizer. The seaweed liquid fertilizer disclosed by the invention is convenient to transport, can be applied to roots of crops and leaves as well, can be used with drip irrigation systems in large-size planting areas, and is very applicable to water-fertilizer integral systems. Large field drought testing results show that by adopting the seaweed liquid fertilizer, the yield of Chinese flowering cabbage can be remarkably increased by 14.7-19.8% when being compared with that of Chinese flowering cabbage with a conventional fertilizer.

The present invention provides a process for the preparation of dialkyl carbonates of general formula (1) wherein R=alkyl (C1-C8) or C6H5CH2 the said process comprises a reaction in between cyclic carbonate and alcohol, in the presence of a solid, double metal cyanide complex catalyst, at a temperature in the range of 140-180° C., for a period of 4-10 hrs, followed by the separation of catalyst from the above said reaction mixture by known methods to obtain the desired product.

The compositions and methods of the present invention comprise the efficient and effective presentation of antigens to the appropriate components of the immune system resulting in the production of species-specific antibodies in vitro. In general, these compositions comprise one or more antigenic components together with a colloidal metal, optionally combined with derivatized PEG (polyethylene glycol) or other agents. The invention also comprises novel cytokine cocktails for generating desired antibodies.

The invention provides a method for preparing a liquid enzyme reagent which is used to fast measure the organophosphorus and carbamate toxicity of pesticide residue and its using method. The agent is formed by choline esterase, buffer solution and stabilizer. The preparing method: 1) preparing the liquid enzyme reagent; 2) placing the liquid enzyme reagent into the On Bei bottle and sealing the port after vacating and preserving it inside the ice-box at 4 degree. The liquid enzyme reagent can be stored at indoor temperature above 15 days so that the activity loss is below 10%, which can fast measure the methylene oxide of the sea foods, the flour, the fried bean milk cream rolls and the dry fruit by matching the bottom object reagent, the color reagent and the buffer solution reagent.

A liquid or liquid-frozen composition comprising: a modified vaccinia Ankara (MVA) virus or variant or derivative thereof and mannitol, wherein mannitol is the sole stabilization agent of the composition. The mannitol may provide a stabilizing effect at 0 to +10° C. or in a liquid-frozen composition, for example between −10° C. and −30° C. or between −20° C. and −23.5° C. The MVA may be used as a vaccine or for use in gene therapy, virotherapy, immunotherapy, or cancer therapy in a mammal, preferably a human.

A process for preparing high stability, high activity biocatalytic materials is disclosed and processes for using the same. The process involves coating of a material or fiber with enzymes and enzyme aggregate providing a material or fiber with high biocatalytic activity and stability useful in heterogeneous environments. In one illustrative approach, enzyme “seeds” are covalently attached to polymer nanofibers followed by treatment with a reagent that crosslinks additional enzyme molecules to the seed enzymes forming enzyme aggregates thereby improving biocatalytic activity due to increased enzyme loading and enzyme stability. This approach creates a useful new biocatalytic immobilized enzyme system with potential applications in bioconversion, bioremediation, biosensors, and biofuel cells.

The present invention relates to a complex face mask comprising: a complex face mask, which comprises a face mask containing effective ingredients wherein the effective ingredients are attached to the face mask in freeze-drying form; and a skin care solution. Also disclosed is a method of producing a complex face mask, which comprises dousing a face mask cloth in a solution containing effective ingredients and then freeze-drying the face mask; and preparing a skin care solution. The effective ingredients attached to the freeze-drying face mask can fully show their effects so that the skin condition can be improved.

Disclosed herein are techniques for accumulating a human T-cell epitope in rice albumen, particularly a method of directly accumulating a T-cell epitope-linked peptide, such as 7 Crp, in rice seeds and a method of inserting 7 Crp into a variable region of glutelin, the major storage protein of rice, to express and accumulate 7 Crp as a part of the glutelin storage protein. Rice producing the T-cell epitope-linked peptide developed in accordance with the present invention is expected to function as an edible vaccine against Japanese cedar pollinosis.

The present invention relates to novel human coagulation Factor VII / VIIa proteins having coagulant potential / activity as well as pharmaceutical compositions comprising the polypeptides, uses and methods of treatment. In particular, the present invention relates to novel, semi synthetic analogues of human coagulation Factor VII and VIIa (FVII and FVIIa) as well as to a method of their production.

The invention discloses a production technology and a mixed emulsion device for improving the stability of a soldering flux for soldering paste. According to the production technology and the mixed emulsion device, mixed emulsion is carried out at low temperature, so that rosin can be effectively prevented from volatilizing and being oxidized at high temperature, and the activity of the welding flux can be kept to the greatest extent; air in the welding flux can be exhausted by adopting low vacuum degree, so that not only can gas in the welding flux be effectively exhausted, but also active ingredients in the welding flux can be prevented from volatilizing at high vacuum degree to lose activity, and the activity of the welding flux can be enhanced; two times of grinding process are adopted, wherein the prepared uniform mixed solution stands for 24 hours after the first time of grinding, so that rosin crystals in the welding flux can be sufficiently separated out, and then the second time of grinding is carried out, and large crystals are ground to meet the production requirements, so that the performance stability of the welding flux is improved; by adopting a manner of increasing scrapers, the mixed emulsion device can scrap sticky and thick objects on the inner wall of and at the bottom of a container into mixed liquid, so that the purpose of fully mixing can be reached, and the performance stability of the welding flux can be greatly improved.

The present invention relates to a method for immobilizing enzymes in a solid porous support, and to enzymes immobilized in solid porous supports. Specifically, the present invention uses a fluid containing the enzyme under high pressure (such as 25 to 50 Mpa) to immobilize the enzyme (such as CALB) on the porous support (such as silica). The resulting porous support contain more immobilized enzyme than would otherwise have been obtained by a process carried out at atmospheric pressures.

The startup of a tubular reactor containing fresh or regenerated molecular sieve catalyst and cooled by steam generation on the shell side, as part of an olefin oligomerisation process, is improved by, during the startup phase of the reactor, controlling the pressure on the shell side of the reactor at maximum 10 barg. The startup may be further improved by controlling the inlet temperature of the hydrocarbon startup stream to the reactor, by controlling the flow of reactant olefins to the reactor, or by controlling the nature and / or concentration of the olefins in the reactor feed.

The present invention discloses a composite microbial agent fermented organic and inorganic biological compound fertilizer, and belongs to the technical field of agricultural fertilizer. The compound fertilize is characterized by consisting of the following materials by weight: 50-55 parts of a semi-finished product, 35-40 parts of an inorganic nutrient, 8-12 parts of weathered coal, and a fermentation body accounting for 1.0-2.0% of the total weight of the above, wherein the biological composite enzyme accounts for 0.01-0.02% of the total weight of the semi-finished product. The present invention has the beneficial effects that microbial fermentation technology is utilized to convert organic materials and chemical fertilizers into organic ingredients, through bioconversion, so as to improve the utilization of fertilizers as well as make crops absorb the necessary nutrients faster and more timely. Chemical nutrients and composite microbial agents are added in a secondary fermentation process, the decreased temperature of the secondary fermentation and microbial fermentation are used to convert inorganic elements, in order not only to avoid the inorganic nutrient loss in high temperature fermentation but also improve the survival rate of microorganisms.

The invention discloses a composite microcapsule wall material, microcapsules and a preparation method of the microcapsules. The composite microcapsule wall material consists of colloid with positive charges and colloid with negative charges, wherein the colloid with positive charges refers to an epsilon-polylysine-vitamin E succinate amide complex. The epsilon-polylysine-vitamin E succinate amide complex serves as one component in the composite microcapsule wall material, and according to the prepared microcapsules, the stability and oxidization resistance of the core material can be obviously improved, so that the core material is difficult to deteriorate, and the quality guarantee period is prolonged.

Nucleic acid sequences encoding chromodomain helicase DNA-binding (CHD) proteins, CHD proteins, and antibodies thereto and methods of use thereof are disclosed.

The invention discloses a method for constructing an implant active surface with a high-content BMP / RGD coating. According to the method, a porous rough surface covered with titanium hydroxy is prepared through a surface treatment method combined with abrasive blasting, acid etching and alkali heat treatment processes, an I-type collagen / BMP-2 composite coating with positive charges and an HA / RGD coating with negative charges are alternately and repeatedly introduced on the porous rough surface so as to form multiple layers of molecular coatings which are arranged sequentially, the load capacity of BMP and RGD in the coatings are greatly improved, the coatings are formed mainly dependent on electrostatic attraction between positive ions and negative ions, the stability of BMP and RGD in the coatings is high, the implant after implanted is slowly and continuously released along with degradation of each coating, and the adhesion, proliferation and differentiation of cells, ossification and early bone healing of the implant and peripheral bone tissues are promoted.

A liquid or liquid-frozen composition comprising: a modified vaccinia Ankara (MVA) virus or variant or derivative thereof and mannitol, wherein mannitol is the sole stabilization agent of the composition. The mannitol may provide a stabilizing effect at 0 to +10° C. or in a liquid-frozen composition, for example between −10° C. and −30° C. or between −20° C. and −23.5° C. The MVA may be used as a vaccine or for use in gene therapy, virotherapy, immunotherapy, or cancer therapy in a mammal, preferably a human.

The compositions and methods of the present invention comprise the efficient and effective presentation of antigens to the appropriate components of the immune system resulting in the production of species-specific antibodies in vitro. In general, these compositions comprise one or more antigenic components together with a colloidal metal, optionally combined with derivatized PEG (polyethylene glycol) or other agents. The invention also comprises novel cytokine cocktails for generating desired antibodies.

The present invention relates to a method for immobilizing enzymes in a solid porous support, and to enzymes immobilized in solid porous supports. Specifically, the present invention uses a fluid containing the enzyme under high pressure (such as 25 to 50 Mpa) to immobilize the enzyme (such as CALB) on the porous support (such as silica). The resulting porous support contain more immobilized enzyme than would otherwise have been obtained by a process carried out at atmospheric pressures.

The invention discloses a method for preparing an enzyme labeled antibody by utilizing a metal organic framework material. The method comprises the following steps: reacting carboxyl on a metal organic framework material PCN-333 with EDC and sulfo-NHS, functionalizing the PCN-333, adding an antibody, carrying out an acylation reaction on amino on the antibody and the functionalized PCN-333 so as to produce a stable amido bond, namely the antibody is immobilized onto the surface of the PCN-333; and finally, encapsulating the enzyme into the PCN-333, and forming an enzyme labeled antibody complex. According to the method disclosed by the invention, by virtue of a coupled reaction between the antibody and MOFs (Metal Organic Frameworks), tedious and high-efficiency enzyme and protein (antibody) chemical crosslinking is avoided, the prepared complex can be separated from excessive antibodies or enzymes by virtue of simple centrifugation, tedious separation and purification steps are avoided, such as gel filtration, long-term dialysis and the like, the activity of the enzymes and antibodies is further prevented from being lost, the coupling process between the antibody and the enzyme inthe traditional meaning is changed, the enzyme labeled antibody process is simplified, the enzyme labeled quantity is improved, and the cost of preparing the enzyme labeled antibody is greatly reduced.

The invention provides a green process for direct oxidation of a large number of substituted or unsubstituted cinnamaldehydes or cinnamyl alcohols into the corresponding alkyl or aryl cinnamates in one step. The process of the present invention is a convenient and efficient green process for the preparation of various aryl or alkyl cinnamates under conventional, microwave and ultrasound directly from cinnamaldehydes or cinnamyl alcohols in the presence of an oxidizing agent, catalyst and an alcohol, with or without an organic solvent. These esters are immensely important compounds in flavor, perfumery and pharmaceutical industries. There are several prior arts available for the preparation of cinnamic esters, but all of them suffer from deficiencies such as use of expensive reagents and catalysts, harsh reaction conditions, use of toxic chemicals and others. In contrast, the present methodology is extremely simple and involves reaction of the substrate with an oxidizing agent mixed with a homogeneous or heterogeneous catalyst and an alcohol with or without organic solvent by stirring at room temperature or refluxing or under microwave or ultrasound irradiation to get the requisite products.