34 results about "Glutelin" patented technology

The invention discloses a method for improving the starch digestibility inhibition effect of a spray drying method by using high amylose, and belongs to the technical field of food processing. According to the invention, highland barley glutelin, low-amylose-content corn starch, normal-amylose-content corn starch and high-amylose-content corn starch are selected, and a spray drying technology is used to simulate an embedding structure of protein in a natural grain structure on the surface of starch particles. The method comprises the following steps: dissolving protein in a 0.02% NaOH solution, then adding corn starch with different amylose contents, stirring for 30 minutes, then carrying out spray drying treatment, controlling the addition amount of the protein to be 10% of the total mass of the starch, setting the inlet temperature of a spray dryer to be 170 DEG C, setting the outlet temperature to be 80 DEG C, and controlling the feeding speed of a peristaltic pump to be 35 mL / min; the appearance of the prepared starch sample is similar to the spatial structure in highland barley grains, and the digestibility of the highland barley grains is reduced.

The invention discloses an application of a TaSnRK1alpha gene in regulating and controlling the high-molecular-weight glutelin content of wheat. The high-molecular-weight glutelin content of the wheat is increased by increasing the TaSnRK1alpha gene in the wheat. In addition, the invention also discloses a TaSnRK1 alpha gene overexpression vector, the vector is obtained by introducing a pWMB110 recombinant vector containing a TaSnRK1 alpha-FLAG expression cassette driven by a Ubi promoter, the efficient expression of the TaSnRK1 alpha gene in wheat can be realized by utilizing the overexpression vector, and the high molecular weight glutelin content in mature grains of the TaSnRK1 alpha gene is obviously improved compared with that of a wild type. New germplasm resources and efficient technical path support are provided for breeding of the high-gluten wheat, the breeding period is shortened, and the method has important agricultural application value.

The invention relates to a method for creating a rice material with low absorbable protein content and remarkably thickened endosperm cell walls and application of the rice material. Specifically, the invention provides a method for improving rice, and improved rice with unique characters is obtained by down-regulating the expression of glutelin genes and down-regulating the expression of globulin genes. In the improved rice, the content of absorbable protein is low, and endosperm cell walls are remarkably thickened. Besides, the improved rice is low in in-vitro digestion rate, has the potential postprandial blood sugar control effect and is suitable for preparing auxiliary food for treating chronic kidney disease patients, diabetic patients and the like.

The present invention relates to a frozen food product coated with a coating, the coating comprising a compound selected from acacia gum, arabic gum, gellan gum, guar gum, locust bean gum, konjac gum, tara gum, a wax, an alginate, cellulose, whey protein isolate, corn zein, gluten, soy, caseinate, chitosan, carrageenan, gelatin, pullulan, modified starch, or combinations thereof.

The invention provides a preparation method of a mixed biomass carbon coated silicon-based negative electrode material. Biomass carbon is mainly derived from prolamin, glutenin and high-activity yeast. Under the catalysis of high-activity yeast, prolamin and glutenin meet water to form a net-shaped structure, and the flexibility of the carbonized porous negative electrode material can be adjusted through different addition proportions, so that the flexible porous nitrogen-doped carbon-coated silicon-based negative electrode material can be obtained, the problem of volume expansion in the electrochemical process of a silicon negative electrode is relieved, and meanwhile, the conductivity is improved; the excellent electrochemical performance is shown. The porous silicon-based negative electrode material with controllable flexibility is obtained through drying and high-temperature annealing, the method is simple, effective and low in cost, and the prepared composite silicon-based negative electrode material is excellent in performance and suitable for large-scale production.

The application provides a low-gluten high-dietary fiber excellent flavor rice as well as a breeding method and application thereof, and relates to the technical field of rice breeding. The breeding method uses a high-dietary fiber rice variety as a female parent and a low-gluten flavor rice variety as a male parent to perform hybridization, and then performs genotype detection on the F2 generation by using Badh-M2 and Lgc1 molecular markers respectively, and combines with agronomic trait selection to realize efficient polymerization of three functional genes of flavor, low gluten and high dietary fiber. The method significantly improves the selection efficiency, avoids trait loss and cycle extension caused by multiple backcrossing. The bred rice has the effects of rich flavor, significantly reduced rice gluten content, obviously increased dietary fiber content and reduced blood glucose generation index, and meanwhile, maintains the effects of high yield, early maturity, excellent plant leaf morphology and good ecological adaptability, and can meet the nutritional needs of chronic kidney disease, diabetes and obese people for functional staple food.

The invention discloses a TaMYB44 gene overexpression vector and application of the TaMYB44 gene overexpression vector, the vector takes pWMB110 as a skeleton vector, and comprises an expression cassette for regulating TaMYB44-FLAG expression by a Ubi promoter. The wheat vector with the function of overexpressing the TaMYB44 gene is constructed, and the TaMYB44 gene in the wheat is overexpressed through genetic transformation, so that the content of high-molecular-weight glutelin in the wheat can be remarkably reduced, a material is provided for wheat quality breeding, and the wheat vector can be used for creating a weak gluten wheat germplasm material.

The invention discloses an application of a rice phosphorus transporter gene and an encoding protein thereof in improving nutrient utilization efficiency and rice quality. According to the invention, OsPHT1 is mutated or inhibited; 1 gene expression can significantly improve nutrient utilization efficiency and rice taste quality and nutritional quality. The method is mainly embodied in that accumulation of nitrogen and phosphorus nutrients of rice is remarkably reduced, the total protein content, glutelin content, prolamin content, globulin content, albumin content and phytic acid content of rice grains are remarkably reduced, the gel consistency of the rice is increased, the reduction value is reduced, and the eating quality and the nutritional quality of the rice are further improved.

The invention discloses a molecular marker and a primer group for detecting high-molecular-weight glutenin subunits of wheat and application of the molecular marker and the primer group, and belongs to the technical field of molecular marker-assisted breeding. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO.3, and T / A mutation exists at the 21st base of the molecular marker; the wheat high-molecular-weight glutenin subunit of which the mutation site is T is Ax1, and the wheat high-molecular-weight glutenin subunit of which the mutation site is A is Ax2 *. The specific KASP molecular marker is developed on the basis of variation sites of Ax1 and Ax2 * subunit gene coding regions in a wheat genome, the Ax1 and Ax2 * subunit types of the wheat high-molecular-weight glutenin can be detected by utilizing the molecular marker, the identification result is accurate and reliable, the repeatability is good, the identification process of the high-molecular-weight glutenin subunit in wheat offspring is greatly simplified, and the application prospect is wide. And the identification efficiency and accuracy are improved.

The application discloses a protein, a gene and application for regulating insoluble gluten content of wheat, and relates to the technical field of biology.The amino acid sequence of the protein is shown as SEQ ID NO.1.The nucleotide sequence of the gene is shown as SEQ ID NO.3.The gene IGC-7B is obtained through fine positioning and map-based cloning.The gene negatively regulates the insoluble gluten content of wheat.The gene IGC-7B is knocked out in wheat through CRISPR-Cas9 technology, and an EMS excellent variation mutant is created in a wheat cultivar to study the function, it is found that the insoluble gluten content of wheat is increased after the gene is mutated, and the grain weight and yield are increased, and the function is verified through haplotype analysis in an AM3 wheat natural population and a rice natural population.The gene IGC-7B can realize the synergistic improvement of grain quality and yield.

The invention relates to the technical field of crop breeding, and particularly discloses a wheat translocation chromosome and a molecular marker and application thereof, and the translocation chromosome comprises a 1AS. 1AL-1DLDx5 + Dy10 translocation chromosome formed by replacing a 475.8-598.6 Mb segment of a long arm end of a 1A chromosome with a 376.6-498.6 Mb segment of a wheat 1D chromosome long arm end. According to the invention, a 1AS.1AL-1DLDx5 + Dy10 translocation chromosome containing a Dx5 + Dy10 high-quality high-molecular-weight glutenin subunit gene is created, and a matched molecular marker is developed, so that the problems that in existing wheat breeding, excellent genes are lacked, specific translocation line identification depends on a time-consuming and labor-consuming cytological method, and high-throughput application is difficult are solved; according to the method, a 1AS.1 1AL-1DLDx5 + Dy10 translocation chromosome carrier and a non-carrier are rapidly and accurately distinguished, traditional cytological identification is replaced, molecular marker-assisted breeding is supported, and the breeding process of a new variety of high-quality wheat is accelerated.

The invention discloses a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 (CRISPR associated protein 9) gene editing vector targeting a wheat TaSnRK1 alpha gene and application of the CRISPR / Cas9 gene editing vector. PBUE414 is used as a skeleton vector of the vector, and the vector comprises a Cas9 expression cassette and an sgRNA (single guide ribonucleic acid) expression cassette; the Cas9 expression cassette is an expression cassette for regulating and controlling Cas9 expression by a Ubi promoter, and the sgRNA expression cassette sequentially comprises the following elements from upstream to downstream: a TaU3 promoter, sgRNA1 of TaSnRK1 alpha, a terminator OsU3t, a TaU3 promoter, sgRNA2 of TaSnRK1 alpha and a terminator TaU3t. The vector is suitable for reducing the high-molecular-weight glutelin content of wheat mature grains through a gene knockout technology, and a new technical means and germplasm resources are provided for wheat quality improvement.

The present disclosure relates to cheese mimicks comprising prolamin and dehydrated microbial cells and methods of making the same. The imitation cheese comprising alcohol soluble glutenin and anhydratic bacteria cells provided herein has physical properties similar to those of natural cheese, and is more nutritional. The method for preparing a simulated cheese comprising prolamin and anhydrobacteria cells provided herein ensures excellent processability.

The present application relates to a kind of heterogenous amyloid fibril-cyanidin-3-O-glucoside (C3G) complex and its application.The preparation method includes: glutelin and soy protein are dispersed in aqueous phase according to mass ratio 3:7-5:5, and are co-assembled to form heterogenous amyloid fibril under acid heat condition;The heterogenous amyloid fibril solution is mixed with C3G solution, and is dried, to obtain heterogenous amyloid fibril-C3G complex.The complex can reduce the hydrolysis rate of rice starch under in vitro digestion condition after being compounded and gelatinized with rice starch.

The present application relates to a kind of covalent bond induced gluten-pectin hydrogel and its preparation method and application, the preparation method specifically is: after reducing treatment to gluten, obtain the reduced gluten solution;Again, with sulfhydryl pectin solution is fully mixed to be uniform;Then, dialysis is carried out in weak alkaline buffer solution, and gluten-pectin hydrogel is obtained.The preparation method of the present application is simple, the operation time is short, does not need oxidant, and the rheological property of the obtained hydrogel enhances pectin gel, and the responsiveness is enhanced.

The invention provides a method for cultivating wheat suitable for processing cakes, which comprises the following steps of: hybridizing wheat materials deleted by different high-molecular-weight glutenin and wheat materials deleted by different alcohol-soluble proteins, carrying out molecular marker-assisted selection on offspring to obtain a series of new deletion bodies deleted by the high-molecular-weight glutenin and the alcohol-soluble proteins, and carrying out high-molecular-weight glutenin and alcohol-soluble protein co-deletion. Through cake processing quality determination, the gluten protein deletion body with excellent cake processing quality is screened out.

A bouillon tablet comprising salt, sweetener, oil and / or fat and flavors, characterized in that the bouillon tablet comprises an edible coating comprises a coating material selected from the group consisting of gelatine, gellan gum, waxes, pullulan, whey protein isolate, cellulose, alginates, corn zein, gluten, soy protein, caseinate, chitosan, pectin, carrageenan, xanthan gum, acacia (arabic) gum, agar, guar gum, modified starch, locust bean gum, konjac gum, and tara gum, or combinations thereof. The tablet may be a biconvex tablet having an upper convex surface (401, 501) and a lower convex surface (405, 505) connected by a cylindrical band (403, 503). The edible coating may comprise one or more herbs or spices.

The invention discloses a lactic acid bacteria microcapsule and a preparation method thereof, and relates to the technical field of lactic acid bacteria microcapsules, the preparation method comprises the following steps: adding a vinasse glutelin solution into pullulan for conjugation reaction, and freeze-drying to obtain a conjugate; adding cell wall powder, a conjugate, okra polysaccharide and a blackcurrant anthocyanin water extract, and carrying out ultrasonic dispersion to obtain an inner layer coating wall material liquid; adding lactic acid bacteria sludge into the inner-layer coating wall material liquid, uniformly stirring, homogenizing and carrying out ultrasonic treatment to obtain inner-layer coating emulsion; adding hydroxypropyl methyl cellulose phthalate and sodium alginate into deionized water, and adding a calcium chloride solution to obtain an outer layer wall material solution; and mixing the inner-layer coating emulsion with the outer-layer wall material solution, homogenizing at high pressure, drying, sealing in vacuum, keeping out of light, and storing in an environment of 4-5 DEG C to obtain the lactic acid bacteria microcapsule.

The invention discloses a method for predicting freezing resistance of wheat frozen dough based on a multiplex PCR molecular marker technology, and belongs to the technical field of molecular biology. According to the invention, a specific multiplex PCR system is constructed, and a high-molecular-weight glutenin subunit combination related to dough freezing resistance is synchronously identified. The method provided by the invention comprises the following steps: extracting wheat genome DNA by using an improved CTAB method; and carrying out synchronous amplification and identification on the subunits of the sites Glu-A1 and Glu-D1. According to the invention, efficient and rapid integrated detection of a plurality of high-quality subunits is realized, and reliable technical support is provided for directional breeding of wheat varieties with excellent frozen dough quality.

The kit (100) comprises a preparation solution (17) configured to prepare a compound to be analyzed, a sensor (1) for determining the gluten contamination in the compound and a processing unit connected thereto. The sensor comprises a base layer (2) having a pair of electrodes (2a); an upper layer (4) having a membrane (4a) permeable to at least one gas releasable from the compound according to the concentration of at least one prolamin present; and a resistive layer (3) interposed between the base layer (2) and the upper layer (4) and configured to detect the gas released by the compound and permeating through the membrane (4a) and to change its resistivity according to the gas detected. The pair of electrodes (2a) is configured to detect the change in resistivity in the resistive layer (3) and to generate a signal according to the change in resistivity detected.

The current invention concerns preparations comprising probiotic strains belonging to the genera Bacillus sp., Lactobacillus sp., and optionally also Pediococcus sp. as viable cells or cytoplasmic extract thereof, and proteases and their use for safe gluten degradation in humans and during food production.

The purpose of the present invention is to provide a food product having improved stickiness and meltability in the mouth as compared with food products produced from GA-SX wheat flour.　The problem is solved by altering the allele at a high-molecular-weight glutenin gene Glu-D1 of GA-SX wheat (a wheat in which the enzymatic activity of GBSSI-A1 is not deficient, the enzymatic activities of GBSSI-B1 and GBSSI-D1 are deficient, and the enzymatic activities of any two of SSIIa-A1, SSIIa-B1 and SSIIa-D1 are deficient) to a d-type allele.

The invention discloses a Glu-1Ey gene for regulating and controlling wheat protein content, a protein and application of the Glu-1Ey gene. The nucleotide sequence of the Glu-1Ey gene is shown as SEQ ID No. 1. According to the invention, an overexpression vector for transforming the agropyron elongatum Glu-1Ey gene is successfully constructed by using a gene overexpression technology, and a strain for overexpressing the Glu-1Ey gene is obtained by using an agrobacterium-mediated wheat immature embryo genetic transformation method. The protein content analysis of transgenic wheat proves that the high-molecular-weight glutelin y-type subunit gene Glu-1Ey of elytrigia elongata can improve the protein content and glutelin content of wheat. The technical scheme provided by the invention has important significance for obtaining high-quality wheat new germplasm by utilizing the high-molecular-weight glutelin y-type subunit gene Glu-1Ey of elytrigia elongata.

Provided are compositions and methods for alleviating symptoms or treating gluten-related diseases. In some embodiments, the compositions include colostrum from cows that are immunized with gluten or partially digested gluten.

The application belongs to the technical field of ecological fertilizer, and relates to a corn gluten protein microbial ecological fertilizer and a preparation method thereof. The microbial ecological fertilizer is prepared from the following components in parts by weight: 15-25 parts of corn gluten protein, 10-20 parts of corn gluten protein amino acid, 6-10 parts of reducing sugar, 15-25 parts of compound microbial agent and 10-20 parts of humic acid. The microbial ecological fertilizer can significantly reduce the occurrence of crop diseases and insect pests, promote the growth of plants, and improve yield and quality.

The invention discloses a method for improving the starch digestion inhibition effect of protein, and belongs to the technical field of food processing. Highland barley starch, highland barley prolamin, highland barley glutelin and highland barley separation are selected as raw materials, and the embedding structure of protein in a natural grain structure on the surface of starch particles is simulated by using a spray drying technology. The preparation method comprises the following steps: dissolving protein in 0.02% NaOH solution, then adding highland barley starch, stirring for 30 minutes, then carrying out spray drying treatment, controlling the addition amount of the protein to be 10% of the total mass of the starch, setting the inlet temperature of a spray dryer to be 170 DEG C, setting the outlet temperature to be 80 DEG C, and controlling the feeding speed of a peristaltic pump to be 35 mL / min, the appearance of the prepared starch sample is similar to the spatial structure in highland barley grains, and the digestibility of the highland barley grains is reduced.

The invention discloses application of a CgPEPC gene in increasing the protein content of rice grains, and relates to the technical field of gene engineering, and an amino acid sequence coded by the CgPEPC gene is shown as SEQ ID NO.3. According to the invention, a CgPEPC gene overexpression rice plant is created, compared with a wild type, the increase of the single gene expression quantity can cause the increase of the protein content of rice grains, and the synthesis of glutelin and alcohol-soluble protein in the grains is enhanced by adjusting the distribution mode of nutrient substances in the rice grains, so that the yield of the rice grains is improved. And no adverse effect is generated on agronomic characters such as rice grain length, grain width, thousand grain weight and the like. The disclosure of the gene function has huge application potential and prospect for the improvement of rice variety traits, and a new gene resource is provided for improving the protein content and nutritional quality breeding of rice.

The present invention relates generally to the field of packaged food products. In particular, the present invention relates to a packaged food product, wherein the package comprises at least one edible packaging layer, and the barrier properties of the package are provided primarily by the edible packaging layer. For example, the present invention relates to a packaged food concentrate, said package comprising an edible primary packaging in contact with said food concentrate, and a secondary packaging, wherein said edible primary packaging provides the required barrier properties, and wherein said edible primary packaging comprises a compound selected from the group consisting of: a gellan gum, a wax, a whey protein isolate, a cellulose, an alginate, a methylcellulose, a hydroxypropyl methylcellulose, a zein, a gluten, a soy protein, a caseinate, a chitosan, a pectin, a carrageenan, or a combination thereof.

This invention discloses a method for creating high-molecular-weight glutenin subunit mutants using single-base editing and its applications. The method includes the following steps: constructing a single-base editing vector by designing sgRNA targeting the tyrosine codon of the HMW-GSs gene; achieving wheat genetic transformation using Agrobacterium-mediated transformation; realizing targeted single-base substitution at target sites (such as position 70 of the 1Bx20 gene and position 73 of the 1Dx5 gene, TAC→TGC), precisely mutating tyrosine to cysteine; and determining bread-processing-related quality parameters of the mutants. This invention uses an adenine base editor to perform targeted editing of the tyrosine codon in wheat HMW-GSs, precisely mutating it to cysteine. This invention is the first to achieve precise editing of HMW-GSs tyrosine to cysteine ​​in wheat, providing a directly applicable theoretical basis and technical path for creating high-quality new wheat germplasm and optimizing bread production processes.