86 results about "Glutelin" patented technology

The invention belongs to the technical field of cereal protein processing, and particularly relates to wheat gliadin with good color and luster and glutenin with high solubility as well as a preparation method and application of the wheat gliadin and the glutenin. According to the method, a cold water solubility control coupling one-step freezing purification technology is broken through, the concentration of ethanol and the temperature of the solution are controlled through cold water, the dissolving state and aggregation behavior of wheat gliadin are synchronously regulated and controlled, then freezing treatment and a one-step method are coupled to achieve controllable protein aggregation, precipitation and freezing, and the purposes of separation and purification are achieved. The technology is green, efficient, simple, convenient and energy-saving, the obtained alcohol-soluble protein is good in color and luster, and industrial production is easy to realize. Furthermore, a membrane separation coupling high-pressure microjet technology is broken through, and on the basis of a technology for obtaining a glutenin concentrated solution through membrane separation, the protein concentrated solution is directly treated by adopting an alkaline condition synchronous high-pressure microjet circulating modification method, so that the structural characteristics such as the scale and the surface hydrophobicity of the target protein and the aggregation behavior are further regulated and controlled; the glutenin is endowed with better solubility.

The invention belongs to the field of lithium ion battery materials, and discloses a carbon source precursor, a phosphate positive electrode material and a preparation method and application of the phosphate positive electrode material, the carbon source precursor comprises glutenin and gliadin, and the mass ratio of the glutenin to the gliadin is (0.65-1): 1. According to the present invention, glutenin and gliadin are subjected to dehydration decomposition and carbonization to form a ring, the three-dimensional network skeleton structure is converted into the uniformly distributed carbon network coating layer, and the phosphate positive electrode material particles dispersed in the three-dimensional network skeleton structure are coated in situ, such that the overgrowth during the grain high-temperature sintering can be easily limited; secondly, a carbon network coating layer formed by carbonization and cyclization of glutenin and gliadin is in a carbon film shape and a carbon filament shape, and the surfaces of crystal grains are uniformly coated with a carbon film; the carbon filaments are uniformly dispersed on the surfaces of the phosphate positive electrode material particles and among the particles to form a carbon network skeleton, so that the electronic conductivity of the phosphate positive electrode material is improved, and the electrochemical performance of the battery is further improved.

The invention relates to soybean protein isolate-glutenin hydrolysate mixed gel and a preparation method and application thereof.The soybean protein isolate-glutenin hydrolysate mixed gel prepared by mixing different hydrolysates of soybean protein isolate and glutenin, adding TG enzyme and then conducting heating has the advantages of being small in pore diameter, small in size and high in elasticity. The plant-based meat product mixed gel is of a three-dimensional network gel structure which is uniform and compact in structure, the elasticity reaches 99.78 + / -0.01, the hardness reaches 901.81 + / -41.55 g, the chewiness reaches 1526.00 + / -33.30 g, the water retention rate reaches 97.62 + / -0.45%, the preparation process is simple and easy to control, the obtained mixed gel has better performance and structure, more possibilities are provided for preparing plant-based meat products and novel food additives, and the plant-based meat product mixed gel is worthy of popularization and application. The method has important application significance in the food industry.

The invention discloses rice LOC_Os01g68930 The application of genes in regulating the protein content in rice grains relates to the field of genetic engineering technology. LOC_Os01g68930 The CDS sequence of the gene is shown in SEQ ID No. 1, and the amino acid sequence of the protein encoded by it is shown in SEQ ID NO. 2. The present invention obtains OE‑LOC_Os01g68930 Overexpression plants. Experiments have shown that: PLACE_ Os01g68930 The total content of glutenin and alcohol-soluble proteins in the seeds of overexpressing transgenic rice reached 7.88% to 7.97%. In comparison, the protein content of wild-type rice grains was 6.97%, and the protein content of transgenic rice grains increased by 13.1%-14.3%. LOC_Os01g68930 It has been confirmed that this gene can effectively regulate the protein content of rice grains, laying a solid foundation for the subsequent breeding of high-protein rice varieties.

The invention discloses a transcription factor GPC6 for regulating and controlling the protein content of rice grains and application of the transcription factor GPC6. The amino acid sequence of the transcription factor is shown as SEQ ID NO.1. The transcription factor comprises a genome region and a promoter, the nucleotide sequence of the genome region is shown as SEQ ID NO.2, the nucleotide sequence of the promoter is shown as SEQ ID NO.3, the coding sequence (CDS) is shown as SEQ ID NO.4, and the coding sequence (CDS) is shown as SEQ ID NO.3. The transcription factor GPC6 can be used for regulating and controlling the protein content of the rice grains. And comprises all alleles thereof. Whole genome association analysis is carried out on the glutelin content and the total protein content, and a site GPC6 capable of being repeatedly detected is identified on a No.6 chromosome. Gene cloning is carried out, gene resources for rice quality genetic improvement can be enriched, and help is provided for rice quality improvement, especially protein content improvement.

The invention discloses a Keap1 inhibition type rice glutenin antioxidant peptide and a preparation method and application thereof, and relates to the technical field of functional foods.The preparation method of the Keap1 inhibition type rice glutenin antioxidant peptide comprises the following steps that rice glutenin powder and water are mixed to be uniform, pretreated through a physical modification method and then added with water to be properly diluted, pH is adjusted, and the Keap1 inhibition type rice glutenin antioxidant peptide is obtained; and adding protease with hydrophobic amino acid cleavage site specificity to carry out enzymolysis for 1-8 hours at 40-65 DEG C, then adding flavourzyme to carry out enzymolysis for 1-8 hours, carrying out enzyme deactivation and centrifugation, and drying the supernatant to obtain the rice gluten antioxidative peptide. Physical pretreatment is combined with a two-step enzymolysis method to carry out restrictive enzymolysis on the rice glutenin, and the obtained zymolyte is high in antioxidant activity, can inhibit oxidative stress by activating an Nrf2 / Keap1 signal channel, and can be used for preparing antioxidant and anti-aging foods, medicines, health care products and cosmetics.

The application discloses a transcription factor for regulating protein content of rice grains GPC6 and application, the amino acid sequence of the transcription factor is shown as SEQ ID NO. 1, the transcription factor comprises a genomic region and a promoter, the nucleotide sequence of the genomic region is shown as SEQ ID NO. 2, the nucleotide sequence of the promoter is shown as SEQ ID NO. 3, the coding sequence (CDS) is shown as SEQ ID NO. 4, and all alleles thereof are contained. The application identifies a repeatedly detectable locus on chromosome 6 by performing whole genome association analysis on gluten content and total protein content GPC6 . Gene cloning is carried out on the locus, so that the genetic resources for improving rice quality are enriched, and help is provided for improving rice quality, in particular, protein content.

The present invention discloses a plant gluten sorting-related protein OsGPA15, its encoding gene and applications. Through phenotypic analysis of rice gluten sorting mutants gpa15 and preliminary mapping of target genes, the gluten sorting-related protein OsGPA15 was finally cloned. The related protein is composed of the amino acid sequence shown in SEQ ID NO.1, and the nucleotide sequence of the gene is as shown in SEQ ID NO.2 or SEQ ID NO.3. The gluten sorting-related protein of the present invention affects the sorting process of gluten in rice endosperm. By introducing the encoding gene of the protein into plants with reduced mature gluten content, transgenic plants with normal mature gluten content can be obtained. Therefore, the protein and its encoding gene of the present invention can be applied to plant genetic improvement.

The invention discloses a method for improving the starch digestibility inhibition effect of a spray drying method by using high amylose, and belongs to the technical field of food processing. According to the invention, highland barley glutelin, low-amylose-content corn starch, normal-amylose-content corn starch and high-amylose-content corn starch are selected, and a spray drying technology is used to simulate an embedding structure of protein in a natural grain structure on the surface of starch particles. The method comprises the following steps: dissolving protein in a 0.02% NaOH solution, then adding corn starch with different amylose contents, stirring for 30 minutes, then carrying out spray drying treatment, controlling the addition amount of the protein to be 10% of the total mass of the starch, setting the inlet temperature of a spray dryer to be 170 DEG C, setting the outlet temperature to be 80 DEG C, and controlling the feeding speed of a peristaltic pump to be 35 mL / min; the appearance of the prepared starch sample is similar to the spatial structure in highland barley grains, and the digestibility of the highland barley grains is reduced.

The invention discloses an application of a TaSnRK1alpha gene in regulating and controlling the high-molecular-weight glutelin content of wheat. The high-molecular-weight glutelin content of the wheat is increased by increasing the TaSnRK1alpha gene in the wheat. In addition, the invention also discloses a TaSnRK1 alpha gene overexpression vector, the vector is obtained by introducing a pWMB110 recombinant vector containing a TaSnRK1 alpha-FLAG expression cassette driven by a Ubi promoter, the efficient expression of the TaSnRK1 alpha gene in wheat can be realized by utilizing the overexpression vector, and the high molecular weight glutelin content in mature grains of the TaSnRK1 alpha gene is obviously improved compared with that of a wild type. New germplasm resources and efficient technical path support are provided for breeding of the high-gluten wheat, the breeding period is shortened, and the method has important agricultural application value.

The invention discloses an application of an elytrigia elongata gene Glu-1Ex in regulating and controlling the content of wheat protein and glutelin. The nucleotide sequence of the gene Glu-1Ex is as shown in SEQ ID No.1. The invention also discloses an application of the gene Glu-1Ex in regulating and controlling the content of wheat protein and glutelin. According to the invention, an overexpression vector for transforming the agropyron elongatum Glu-1Ex gene is successfully constructed by using a gene overexpression technology, and a strain for overexpressing the Glu-1Ex gene is obtained by using an agrobacterium-mediated wheat immature embryo genetic transformation method. The analysis on the protein content of transgenic wheat proves that the Glu-1Ex gene of elytrigia elongata can improve the protein content and glutelin content of wheat. The technical scheme provided by the invention has important significance for obtaining high-quality wheat new germplasm by utilizing the elytrigia elongata Glu-1Ex gene.

The present invention addresses the problem of providing a method for producing fat-containing noodles having good noodle-making properties and texture even when a large amount of fat is added to the noodles. According to the present invention, by adding water to a raw material powder, kneading the raw material powder for a certain period of time, and then adding fat and oil, the hydration of dough and the formation of glutelin can be performed without being hindered by fat and oil, thereby obtaining fat-and-oil-containing noodles having a good noodle-making property and a good texture. It is preferable that 30-140 ml of the oil or fat is added to 1 kg of the main raw material powder, and it is preferable that the oil or fat is added for a period of 3-12 minutes after the start of kneading as the time for which the oil or fat is added. In addition, the fat-containing noodles according to the present invention preferably have a multi-layer structure comprising two outer layers and at least one inner layer, and the dough produced by the method is used in the inner layer.

The invention relates to a method for creating a rice material with low absorbable protein content and remarkably thickened endosperm cell walls and application of the rice material. Specifically, the invention provides a method for improving rice, and improved rice with unique characters is obtained by down-regulating the expression of glutelin genes and down-regulating the expression of globulin genes. In the improved rice, the content of absorbable protein is low, and endosperm cell walls are remarkably thickened. Besides, the improved rice is low in in-vitro digestion rate, has the potential postprandial blood sugar control effect and is suitable for preparing auxiliary food for treating chronic kidney disease patients, diabetic patients and the like.

The present invention discloses a bread digestibility improver and its application in the production of enzymolysis-resistant whole-wheat bread, and the field of whole-wheat bread processing. The present invention disperses gluten under alkaline conditions so that the protein chain is fully unfolded and then β-glucan is added, and a highland barley gluten / β-glucan complex is formed by molecular self-assembly to obtain a bread digestibility improver. The obtained bread digestibility improver is mixed with whole-wheat flour and gluten, and the enzymolysis-resistant whole-wheat bread is obtained through the steps of kneading, fermentation, molding, proofing, baking, etc. The present invention regulates the digestibility of starch in whole-wheat bread by adding a bread digestibility improver, greatly improves the resistant starch content in whole-wheat bread, and the obtained bread has a good taste and high nutritional value.

Provided are compositions and methods for alleviating symptoms or treating gluten-related diseases. In some embodiments, the compositions include colostrum from cows that are immunized with gluten or partially digested gluten.

The method comprises the following steps: mixing vital wheat gluten and 65-70% ethanol according to a mass ratio of 1: 20-1: 30, stirring and extracting in a water bath kettle at the temperature of 30-40 DEG C for 2-3 hours at the stirring speed of 1.5-6 m / s; and centrifuging the extracted solution for 5-10 minutes by using a centrifugal machine under the condition of 3500r / min, and taking the lower layer precipitate, namely glutenin. The preparation method comprises the following steps: dissolving glutenin in a saturated urea solution, stirring and dissolving for 10-15 hours at normal temperature, centrifuging for 5-10 minutes under the condition of 3500 r / min to remove precipitate to obtain glutenin-urea compound liquid, and standing at room temperature to separate out urea crystals; and dialyzing the residual protein liquid by using an MD77 dialysis bag to remove small molecular proteins and ions, freeze-drying at-60 DEG C for 2-6 days, crushing, and sieving by using a 100-mesh sieve to obtain powder which is a glutenin monomer, and the preparation rate is 20-50%.

The invention discloses a chitosan wrinkle-inhibiting, beautifying and repairing mask and a preparation method thereof. The chitosan wrinkle-inhibiting, beautifying and repairing mask comprises mask liquid and chitosan fiber cloth, the mask liquid consists of a mixed raw material and water, and the mass fraction of the mixed raw material in the mask liquid is 8-30wt%; the mixed raw materials comprise 10-16 parts by weight of carboxymethyl chitosan, 6-8 parts by weight of polyethylene glycol, 6-15 parts by weight of gibberellin, 12-18 parts by weight of cytokinin, 5-6 parts by weight of propylene glycol, 5-6 parts by weight of sodium hyaluronate, 4-5 parts by weight of glycerol, 2-5 parts by weight of sea buckthorn fruit oil, 1-4 parts by weight of sea buckthorn flavone, 4-6 parts by weight of vitamin E and 2-6 parts by weight of corn glutelin amino acid; the composition comprises, by weight, 2-4 parts of beta-glucan, 20-30 parts of dimethyl hydroxyl silicone oil, 3-6 parts of borneol and 1-3 parts of water. The technical problem that an existing chitosan mask still has defects in the aspects of wrinkle inhibiting, beautifying and repairing can be solved.

The invention discloses a method for extracting and identifying a high molecular weight glutenin subunit (HMW-GS) in wheat grains in an early stage, which comprises the following steps: taking the wheat grains 15 days after flowering and before a dough stage, and extracting by using 80-160 [mu] l of an extraction buffer solution (40% of isopropanol, 200mM of Tris-HCl, pH 8.0, 0.75% of DTT and 0.5% of 4-VP). The extraction and identification method is suitable for early identification of high molecular weight glutenin subunit types in wheat grains, and is also suitable for identification of glutenin subunit types in harvested seeds. The seeds 15 days after flowering and before the grain dough stage are high in moisture content, low in grain hardness and easy to grind, early sampling accelerates the extraction and detection efficiency of the wheat grain high-molecular-weight glutenin subunit, and accelerates the wheat quality breeding process.

The present invention relates to a frozen food product coated with a coating, the coating comprising a compound selected from acacia gum, arabic gum, gellan gum, guar gum, locust bean gum, konjac gum, tara gum, a wax, an alginate, cellulose, whey protein isolate, corn zein, gluten, soy, caseinate, chitosan, carrageenan, gelatin, pullulan, modified starch, or combinations thereof.

The invention provides a preparation method of a mixed biomass carbon coated silicon-based negative electrode material. Biomass carbon is mainly derived from prolamin, glutenin and high-activity yeast. Under the catalysis of high-activity yeast, prolamin and glutenin meet water to form a net-shaped structure, and the flexibility of the carbonized porous negative electrode material can be adjusted through different addition proportions, so that the flexible porous nitrogen-doped carbon-coated silicon-based negative electrode material can be obtained, the problem of volume expansion in the electrochemical process of a silicon negative electrode is relieved, and meanwhile, the conductivity is improved; the excellent electrochemical performance is shown. The porous silicon-based negative electrode material with controllable flexibility is obtained through drying and high-temperature annealing, the method is simple, effective and low in cost, and the prepared composite silicon-based negative electrode material is excellent in performance and suitable for large-scale production.

The invention belongs to the technical field of rice variety breeding, and particularly relates to a breeding method of a special rice variety for patients with kidney diseases and diabetes mellitus, which comprises the following specific steps: selecting a parent material: taking a low-water-soluble protein mutant variety LGC-1 introduced from a radiation breeding field of the Japanese biological resource research institute as a female parent; the Qingyou 308 rice variety bred by Institute of Rice Institute of Academy of Agricultural Sciences in Fujian Province is used as a male parent for hybridization; hybrid progenies are obtained through hybridization, selfing is carried out, progeny seeds are analyzed from F1, and seeds with low glutelin characters and high alcohol-soluble protein characters are screened out; selecting seeds with low glycemic index from the seeds with low glutelin character and high prolamin character; seeds with high amylose content are selected from the seeds with the low glycemic index. The breeding method has the advantages that the bred seeds can be suitable for kidney patients and also suitable for diabetics.

The application provides a low-gluten high-dietary fiber excellent flavor rice as well as a breeding method and application thereof, and relates to the technical field of rice breeding. The breeding method uses a high-dietary fiber rice variety as a female parent and a low-gluten flavor rice variety as a male parent to perform hybridization, and then performs genotype detection on the F2 generation by using Badh-M2 and Lgc1 molecular markers respectively, and combines with agronomic trait selection to realize efficient polymerization of three functional genes of flavor, low gluten and high dietary fiber. The method significantly improves the selection efficiency, avoids trait loss and cycle extension caused by multiple backcrossing. The bred rice has the effects of rich flavor, significantly reduced rice gluten content, obviously increased dietary fiber content and reduced blood glucose generation index, and meanwhile, maintains the effects of high yield, early maturity, excellent plant leaf morphology and good ecological adaptability, and can meet the nutritional needs of chronic kidney disease, diabetes and obese people for functional staple food.

The invention relates to a plant glutelin sorting related protein OsVPS34 as well as a coding gene and application thereof. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO.3 in a sequence table; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of SEQ ID NO.3, is related to plant glutelin sorting and is derived from SEQ ID NO.3. The protein provided by the invention affects the sorting of glutelin in plant endosperm, and the glutelin cannot be correctly transported into a type II protein body, so that the glutelin content in mature seeds is reduced, and a glutelin precursor is increased. The coding gene of the protein is introduced into a plant with abnormal glutelin sorting, and a transgenic plant with normal glutelin sorting can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

The preparation method comprises the following steps: mixing vital gluten and 65% ethanol according to a mass ratio of 1: 30, and stirring and extracting in a water bath kettle at 35 DEG C for 3 hours to dissolve alcohol-soluble protein in the vital gluten in the ethanol; after standing, centrifuging for 5 minutes by using a centrifugal machine under the condition of 3,500 r / min, taking the precipitate, repeating the steps for dissolving, centrifuging the solution for 5 minutes, and taking the precipitate, namely the glutenin aggregate. Dissolving the glutenin aggregate at 30 DEG C for 120 minutes by using 0.1% sodium hydroxide alkali liquor, standing at 50 DEG C for 12 hours, and regulating the pH value to be neutral to generate precipitate; and centrifuging for 5 minutes to obtain a precipitate which is alkali-soluble glutenin. Amylose and alkali-soluble glutenin are mixed according to the mass ratio of 1: 8-1: 10, drinking water accounting for 3%-5% of the starch amount is added, mixing is conducted for 30-50 min at the temperature of 30-35 DEG C, and the disulfide bond content of the alkali-soluble glutenin is reduced by 80% or above.

The invention discloses a TaMYB44 gene overexpression vector and application of the TaMYB44 gene overexpression vector, the vector takes pWMB110 as a skeleton vector, and comprises an expression cassette for regulating TaMYB44-FLAG expression by a Ubi promoter. The wheat vector with the function of overexpressing the TaMYB44 gene is constructed, and the TaMYB44 gene in the wheat is overexpressed through genetic transformation, so that the content of high-molecular-weight glutelin in the wheat can be remarkably reduced, a material is provided for wheat quality breeding, and the wheat vector can be used for creating a weak gluten wheat germplasm material.

The application discloses a new gene GluA1-L1 which is a gene locus positively affecting rice quality, can regulate the content of glutelin in rice endosperm, and is used for creating high-quality rice varieties and providing a gene resource for cultivating high-quality rice and breeding new low-glutelin rice varieties.

The invention discloses an application of a rice phosphorus transporter gene and an encoding protein thereof in improving nutrient utilization efficiency and rice quality. According to the invention, OsPHT1 is mutated or inhibited; 1 gene expression can significantly improve nutrient utilization efficiency and rice taste quality and nutritional quality. The method is mainly embodied in that accumulation of nitrogen and phosphorus nutrients of rice is remarkably reduced, the total protein content, glutelin content, prolamin content, globulin content, albumin content and phytic acid content of rice grains are remarkably reduced, the gel consistency of the rice is increased, the reduction value is reduced, and the eating quality and the nutritional quality of the rice are further improved.

The invention discloses a molecular marker and a primer group for detecting high-molecular-weight glutenin subunits of wheat and application of the molecular marker and the primer group, and belongs to the technical field of molecular marker-assisted breeding. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO.3, and T / A mutation exists at the 21st base of the molecular marker; the wheat high-molecular-weight glutenin subunit of which the mutation site is T is Ax1, and the wheat high-molecular-weight glutenin subunit of which the mutation site is A is Ax2 *. The specific KASP molecular marker is developed on the basis of variation sites of Ax1 and Ax2 * subunit gene coding regions in a wheat genome, the Ax1 and Ax2 * subunit types of the wheat high-molecular-weight glutenin can be detected by utilizing the molecular marker, the identification result is accurate and reliable, the repeatability is good, the identification process of the high-molecular-weight glutenin subunit in wheat offspring is greatly simplified, and the application prospect is wide. And the identification efficiency and accuracy are improved.

A method for increasing starch yield and / or gluten yield from corn kernels in a wet milling process is disclosed, the method comprising contacting a fiber-rich fraction of the milled kernels with an effective amount of SO2 and an effective amount of one or more hydrolytic enzymes during a fiber washing step, wherein at least one of the hydrolytic enzymes is selected from xylanases and / or cellulases.

The invention provides a long-term stable food-grade capillary foam composition and a preparation method and application of a capillary foam system, and belongs to the technical field of food-grade foam. The method comprises the following steps: by taking rice protein powder as a raw material, extracting to obtain rice glutelin through the steps of alkali dissolution and acid precipitation; performing alkali heat treatment on the rice glutelin to obtain rice glutelin colloidal particles; the rice glutelin colloidal particles and water are prepared into a suspension, an oil phase is added into the suspension, and the capillary foam system is obtained after high-speed stirring, dispersing and inflating. The stable period of the capillary foam system is as long as 2 months. The raw materials are wide in source and high in safety and nutritional value, the preparation process is green and safe, the technology is simple, and the raw materials and the preparation method are both suitable for manufacturing stable foam in the field of food processing.