53 results about "Glutelin" patented technology

The application discloses a transcription factor for regulating protein content of rice grains GPC6 and application, the amino acid sequence of the transcription factor is shown as SEQ ID NO. 1, the transcription factor comprises a genomic region and a promoter, the nucleotide sequence of the genomic region is shown as SEQ ID NO. 2, the nucleotide sequence of the promoter is shown as SEQ ID NO. 3, the coding sequence (CDS) is shown as SEQ ID NO. 4, and all alleles thereof are contained. The application identifies a repeatedly detectable locus on chromosome 6 by performing whole genome association analysis on gluten content and total protein content GPC6 . Gene cloning is carried out on the locus, so that the genetic resources for improving rice quality are enriched, and help is provided for improving rice quality, in particular, protein content.

The invention discloses a method for improving the starch digestibility inhibition effect of a spray drying method by using high amylose, and belongs to the technical field of food processing. According to the invention, highland barley glutelin, low-amylose-content corn starch, normal-amylose-content corn starch and high-amylose-content corn starch are selected, and a spray drying technology is used to simulate an embedding structure of protein in a natural grain structure on the surface of starch particles. The method comprises the following steps: dissolving protein in a 0.02% NaOH solution, then adding corn starch with different amylose contents, stirring for 30 minutes, then carrying out spray drying treatment, controlling the addition amount of the protein to be 10% of the total mass of the starch, setting the inlet temperature of a spray dryer to be 170 DEG C, setting the outlet temperature to be 80 DEG C, and controlling the feeding speed of a peristaltic pump to be 35 mL / min; the appearance of the prepared starch sample is similar to the spatial structure in highland barley grains, and the digestibility of the highland barley grains is reduced.

The invention discloses an application of a TaSnRK1alpha gene in regulating and controlling the high-molecular-weight glutelin content of wheat. The high-molecular-weight glutelin content of the wheat is increased by increasing the TaSnRK1alpha gene in the wheat. In addition, the invention also discloses a TaSnRK1 alpha gene overexpression vector, the vector is obtained by introducing a pWMB110 recombinant vector containing a TaSnRK1 alpha-FLAG expression cassette driven by a Ubi promoter, the efficient expression of the TaSnRK1 alpha gene in wheat can be realized by utilizing the overexpression vector, and the high molecular weight glutelin content in mature grains of the TaSnRK1 alpha gene is obviously improved compared with that of a wild type. New germplasm resources and efficient technical path support are provided for breeding of the high-gluten wheat, the breeding period is shortened, and the method has important agricultural application value.

The invention discloses an application of an elytrigia elongata gene Glu-1Ex in regulating and controlling the content of wheat protein and glutelin. The nucleotide sequence of the gene Glu-1Ex is as shown in SEQ ID No.1. The invention also discloses an application of the gene Glu-1Ex in regulating and controlling the content of wheat protein and glutelin. According to the invention, an overexpression vector for transforming the agropyron elongatum Glu-1Ex gene is successfully constructed by using a gene overexpression technology, and a strain for overexpressing the Glu-1Ex gene is obtained by using an agrobacterium-mediated wheat immature embryo genetic transformation method. The analysis on the protein content of transgenic wheat proves that the Glu-1Ex gene of elytrigia elongata can improve the protein content and glutelin content of wheat. The technical scheme provided by the invention has important significance for obtaining high-quality wheat new germplasm by utilizing the elytrigia elongata Glu-1Ex gene.

The present invention addresses the problem of providing a method for producing fat-containing noodles having good noodle-making properties and texture even when a large amount of fat is added to the noodles. According to the present invention, by adding water to a raw material powder, kneading the raw material powder for a certain period of time, and then adding fat and oil, the hydration of dough and the formation of glutelin can be performed without being hindered by fat and oil, thereby obtaining fat-and-oil-containing noodles having a good noodle-making property and a good texture. It is preferable that 30-140 ml of the oil or fat is added to 1 kg of the main raw material powder, and it is preferable that the oil or fat is added for a period of 3-12 minutes after the start of kneading as the time for which the oil or fat is added. In addition, the fat-containing noodles according to the present invention preferably have a multi-layer structure comprising two outer layers and at least one inner layer, and the dough produced by the method is used in the inner layer.

The invention relates to a method for creating a rice material with low absorbable protein content and remarkably thickened endosperm cell walls and application of the rice material. Specifically, the invention provides a method for improving rice, and improved rice with unique characters is obtained by down-regulating the expression of glutelin genes and down-regulating the expression of globulin genes. In the improved rice, the content of absorbable protein is low, and endosperm cell walls are remarkably thickened. Besides, the improved rice is low in in-vitro digestion rate, has the potential postprandial blood sugar control effect and is suitable for preparing auxiliary food for treating chronic kidney disease patients, diabetic patients and the like.

The present invention discloses a bread digestibility improver and its application in the production of enzymolysis-resistant whole-wheat bread, and the field of whole-wheat bread processing. The present invention disperses gluten under alkaline conditions so that the protein chain is fully unfolded and then β-glucan is added, and a highland barley gluten / β-glucan complex is formed by molecular self-assembly to obtain a bread digestibility improver. The obtained bread digestibility improver is mixed with whole-wheat flour and gluten, and the enzymolysis-resistant whole-wheat bread is obtained through the steps of kneading, fermentation, molding, proofing, baking, etc. The present invention regulates the digestibility of starch in whole-wheat bread by adding a bread digestibility improver, greatly improves the resistant starch content in whole-wheat bread, and the obtained bread has a good taste and high nutritional value.

Provided are compositions and methods for alleviating symptoms or treating gluten-related diseases. In some embodiments, the compositions include colostrum from cows that are immunized with gluten or partially digested gluten.

The invention discloses a chitosan wrinkle-inhibiting, beautifying and repairing mask and a preparation method thereof. The chitosan wrinkle-inhibiting, beautifying and repairing mask comprises mask liquid and chitosan fiber cloth, the mask liquid consists of a mixed raw material and water, and the mass fraction of the mixed raw material in the mask liquid is 8-30wt%; the mixed raw materials comprise 10-16 parts by weight of carboxymethyl chitosan, 6-8 parts by weight of polyethylene glycol, 6-15 parts by weight of gibberellin, 12-18 parts by weight of cytokinin, 5-6 parts by weight of propylene glycol, 5-6 parts by weight of sodium hyaluronate, 4-5 parts by weight of glycerol, 2-5 parts by weight of sea buckthorn fruit oil, 1-4 parts by weight of sea buckthorn flavone, 4-6 parts by weight of vitamin E and 2-6 parts by weight of corn glutelin amino acid; the composition comprises, by weight, 2-4 parts of beta-glucan, 20-30 parts of dimethyl hydroxyl silicone oil, 3-6 parts of borneol and 1-3 parts of water. The technical problem that an existing chitosan mask still has defects in the aspects of wrinkle inhibiting, beautifying and repairing can be solved.

The invention discloses a method for extracting and identifying a high molecular weight glutenin subunit (HMW-GS) in wheat grains in an early stage, which comprises the following steps: taking the wheat grains 15 days after flowering and before a dough stage, and extracting by using 80-160 [mu] l of an extraction buffer solution (40% of isopropanol, 200mM of Tris-HCl, pH 8.0, 0.75% of DTT and 0.5% of 4-VP). The extraction and identification method is suitable for early identification of high molecular weight glutenin subunit types in wheat grains, and is also suitable for identification of glutenin subunit types in harvested seeds. The seeds 15 days after flowering and before the grain dough stage are high in moisture content, low in grain hardness and easy to grind, early sampling accelerates the extraction and detection efficiency of the wheat grain high-molecular-weight glutenin subunit, and accelerates the wheat quality breeding process.

The present invention relates to a frozen food product coated with a coating, the coating comprising a compound selected from acacia gum, arabic gum, gellan gum, guar gum, locust bean gum, konjac gum, tara gum, a wax, an alginate, cellulose, whey protein isolate, corn zein, gluten, soy, caseinate, chitosan, carrageenan, gelatin, pullulan, modified starch, or combinations thereof.

The invention provides a preparation method of a mixed biomass carbon coated silicon-based negative electrode material. Biomass carbon is mainly derived from prolamin, glutenin and high-activity yeast. Under the catalysis of high-activity yeast, prolamin and glutenin meet water to form a net-shaped structure, and the flexibility of the carbonized porous negative electrode material can be adjusted through different addition proportions, so that the flexible porous nitrogen-doped carbon-coated silicon-based negative electrode material can be obtained, the problem of volume expansion in the electrochemical process of a silicon negative electrode is relieved, and meanwhile, the conductivity is improved; the excellent electrochemical performance is shown. The porous silicon-based negative electrode material with controllable flexibility is obtained through drying and high-temperature annealing, the method is simple, effective and low in cost, and the prepared composite silicon-based negative electrode material is excellent in performance and suitable for large-scale production.

The invention belongs to the technical field of rice variety breeding, and particularly relates to a breeding method of a special rice variety for patients with kidney diseases and diabetes mellitus, which comprises the following specific steps: selecting a parent material: taking a low-water-soluble protein mutant variety LGC-1 introduced from a radiation breeding field of the Japanese biological resource research institute as a female parent; the Qingyou 308 rice variety bred by Institute of Rice Institute of Academy of Agricultural Sciences in Fujian Province is used as a male parent for hybridization; hybrid progenies are obtained through hybridization, selfing is carried out, progeny seeds are analyzed from F1, and seeds with low glutelin characters and high alcohol-soluble protein characters are screened out; selecting seeds with low glycemic index from the seeds with low glutelin character and high prolamin character; seeds with high amylose content are selected from the seeds with the low glycemic index. The breeding method has the advantages that the bred seeds can be suitable for kidney patients and also suitable for diabetics.

The application provides a low-gluten high-dietary fiber excellent flavor rice as well as a breeding method and application thereof, and relates to the technical field of rice breeding. The breeding method uses a high-dietary fiber rice variety as a female parent and a low-gluten flavor rice variety as a male parent to perform hybridization, and then performs genotype detection on the F2 generation by using Badh-M2 and Lgc1 molecular markers respectively, and combines with agronomic trait selection to realize efficient polymerization of three functional genes of flavor, low gluten and high dietary fiber. The method significantly improves the selection efficiency, avoids trait loss and cycle extension caused by multiple backcrossing. The bred rice has the effects of rich flavor, significantly reduced rice gluten content, obviously increased dietary fiber content and reduced blood glucose generation index, and meanwhile, maintains the effects of high yield, early maturity, excellent plant leaf morphology and good ecological adaptability, and can meet the nutritional needs of chronic kidney disease, diabetes and obese people for functional staple food.

The invention relates to a plant glutelin sorting related protein OsVPS34 as well as a coding gene and application thereof. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO.3 in a sequence table; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of SEQ ID NO.3, is related to plant glutelin sorting and is derived from SEQ ID NO.3. The protein provided by the invention affects the sorting of glutelin in plant endosperm, and the glutelin cannot be correctly transported into a type II protein body, so that the glutelin content in mature seeds is reduced, and a glutelin precursor is increased. The coding gene of the protein is introduced into a plant with abnormal glutelin sorting, and a transgenic plant with normal glutelin sorting can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

The preparation method comprises the following steps: mixing vital gluten and 65% ethanol according to a mass ratio of 1: 30, and stirring and extracting in a water bath kettle at 35 DEG C for 3 hours to dissolve alcohol-soluble protein in the vital gluten in the ethanol; after standing, centrifuging for 5 minutes by using a centrifugal machine under the condition of 3,500 r / min, taking the precipitate, repeating the steps for dissolving, centrifuging the solution for 5 minutes, and taking the precipitate, namely the glutenin aggregate. Dissolving the glutenin aggregate at 30 DEG C for 120 minutes by using 0.1% sodium hydroxide alkali liquor, standing at 50 DEG C for 12 hours, and regulating the pH value to be neutral to generate precipitate; and centrifuging for 5 minutes to obtain a precipitate which is alkali-soluble glutenin. Amylose and alkali-soluble glutenin are mixed according to the mass ratio of 1: 8-1: 10, drinking water accounting for 3%-5% of the starch amount is added, mixing is conducted for 30-50 min at the temperature of 30-35 DEG C, and the disulfide bond content of the alkali-soluble glutenin is reduced by 80% or above.

The invention discloses a TaMYB44 gene overexpression vector and application of the TaMYB44 gene overexpression vector, the vector takes pWMB110 as a skeleton vector, and comprises an expression cassette for regulating TaMYB44-FLAG expression by a Ubi promoter. The wheat vector with the function of overexpressing the TaMYB44 gene is constructed, and the TaMYB44 gene in the wheat is overexpressed through genetic transformation, so that the content of high-molecular-weight glutelin in the wheat can be remarkably reduced, a material is provided for wheat quality breeding, and the wheat vector can be used for creating a weak gluten wheat germplasm material.

The application discloses a new gene GluA1-L1 which is a gene locus positively affecting rice quality, can regulate the content of glutelin in rice endosperm, and is used for creating high-quality rice varieties and providing a gene resource for cultivating high-quality rice and breeding new low-glutelin rice varieties.

The invention discloses an application of a rice phosphorus transporter gene and an encoding protein thereof in improving nutrient utilization efficiency and rice quality. According to the invention, OsPHT1 is mutated or inhibited; 1 gene expression can significantly improve nutrient utilization efficiency and rice taste quality and nutritional quality. The method is mainly embodied in that accumulation of nitrogen and phosphorus nutrients of rice is remarkably reduced, the total protein content, glutelin content, prolamin content, globulin content, albumin content and phytic acid content of rice grains are remarkably reduced, the gel consistency of the rice is increased, the reduction value is reduced, and the eating quality and the nutritional quality of the rice are further improved.

The invention discloses a molecular marker and a primer group for detecting high-molecular-weight glutenin subunits of wheat and application of the molecular marker and the primer group, and belongs to the technical field of molecular marker-assisted breeding. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO.3, and T / A mutation exists at the 21st base of the molecular marker; the wheat high-molecular-weight glutenin subunit of which the mutation site is T is Ax1, and the wheat high-molecular-weight glutenin subunit of which the mutation site is A is Ax2 *. The specific KASP molecular marker is developed on the basis of variation sites of Ax1 and Ax2 * subunit gene coding regions in a wheat genome, the Ax1 and Ax2 * subunit types of the wheat high-molecular-weight glutenin can be detected by utilizing the molecular marker, the identification result is accurate and reliable, the repeatability is good, the identification process of the high-molecular-weight glutenin subunit in wheat offspring is greatly simplified, and the application prospect is wide. And the identification efficiency and accuracy are improved.

The application discloses a protein, a gene and application for regulating insoluble gluten content of wheat, and relates to the technical field of biology.The amino acid sequence of the protein is shown as SEQ ID NO.1.The nucleotide sequence of the gene is shown as SEQ ID NO.3.The gene IGC-7B is obtained through fine positioning and map-based cloning.The gene negatively regulates the insoluble gluten content of wheat.The gene IGC-7B is knocked out in wheat through CRISPR-Cas9 technology, and an EMS excellent variation mutant is created in a wheat cultivar to study the function, it is found that the insoluble gluten content of wheat is increased after the gene is mutated, and the grain weight and yield are increased, and the function is verified through haplotype analysis in an AM3 wheat natural population and a rice natural population.The gene IGC-7B can realize the synergistic improvement of grain quality and yield.

The invention relates to the technical field of crop breeding, and particularly discloses a wheat translocation chromosome and a molecular marker and application thereof, and the translocation chromosome comprises a 1AS. 1AL-1DLDx5 + Dy10 translocation chromosome formed by replacing a 475.8-598.6 Mb segment of a long arm end of a 1A chromosome with a 376.6-498.6 Mb segment of a wheat 1D chromosome long arm end. According to the invention, a 1AS.1AL-1DLDx5 + Dy10 translocation chromosome containing a Dx5 + Dy10 high-quality high-molecular-weight glutenin subunit gene is created, and a matched molecular marker is developed, so that the problems that in existing wheat breeding, excellent genes are lacked, specific translocation line identification depends on a time-consuming and labor-consuming cytological method, and high-throughput application is difficult are solved; according to the method, a 1AS.1 1AL-1DLDx5 + Dy10 translocation chromosome carrier and a non-carrier are rapidly and accurately distinguished, traditional cytological identification is replaced, molecular marker-assisted breeding is supported, and the breeding process of a new variety of high-quality wheat is accelerated.

The invention discloses a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 (CRISPR associated protein 9) gene editing vector targeting a wheat TaSnRK1 alpha gene and application of the CRISPR / Cas9 gene editing vector. PBUE414 is used as a skeleton vector of the vector, and the vector comprises a Cas9 expression cassette and an sgRNA (single guide ribonucleic acid) expression cassette; the Cas9 expression cassette is an expression cassette for regulating and controlling Cas9 expression by a Ubi promoter, and the sgRNA expression cassette sequentially comprises the following elements from upstream to downstream: a TaU3 promoter, sgRNA1 of TaSnRK1 alpha, a terminator OsU3t, a TaU3 promoter, sgRNA2 of TaSnRK1 alpha and a terminator TaU3t. The vector is suitable for reducing the high-molecular-weight glutelin content of wheat mature grains through a gene knockout technology, and a new technical means and germplasm resources are provided for wheat quality improvement.

The present disclosure relates to cheese mimicks comprising prolamin and dehydrated microbial cells and methods of making the same. The imitation cheese comprising alcohol soluble glutenin and anhydratic bacteria cells provided herein has physical properties similar to those of natural cheese, and is more nutritional. The method for preparing a simulated cheese comprising prolamin and anhydrobacteria cells provided herein ensures excellent processability.

The present application relates to a kind of heterogenous amyloid fibril-cyanidin-3-O-glucoside (C3G) complex and its application.The preparation method includes: glutelin and soy protein are dispersed in aqueous phase according to mass ratio 3:7-5:5, and are co-assembled to form heterogenous amyloid fibril under acid heat condition;The heterogenous amyloid fibril solution is mixed with C3G solution, and is dried, to obtain heterogenous amyloid fibril-C3G complex.The complex can reduce the hydrolysis rate of rice starch under in vitro digestion condition after being compounded and gelatinized with rice starch.

The present application relates to a kind of covalent bond induced gluten-pectin hydrogel and its preparation method and application, the preparation method specifically is: after reducing treatment to gluten, obtain the reduced gluten solution;Again, with sulfhydryl pectin solution is fully mixed to be uniform;Then, dialysis is carried out in weak alkaline buffer solution, and gluten-pectin hydrogel is obtained.The preparation method of the present application is simple, the operation time is short, does not need oxidant, and the rheological property of the obtained hydrogel enhances pectin gel, and the responsiveness is enhanced.

The invention provides a method for cultivating wheat suitable for processing cakes, which comprises the following steps of: hybridizing wheat materials deleted by different high-molecular-weight glutenin and wheat materials deleted by different alcohol-soluble proteins, carrying out molecular marker-assisted selection on offspring to obtain a series of new deletion bodies deleted by the high-molecular-weight glutenin and the alcohol-soluble proteins, and carrying out high-molecular-weight glutenin and alcohol-soluble protein co-deletion. Through cake processing quality determination, the gluten protein deletion body with excellent cake processing quality is screened out.

A bouillon tablet comprising salt, sweetener, oil and / or fat and flavors, characterized in that the bouillon tablet comprises an edible coating comprises a coating material selected from the group consisting of gelatine, gellan gum, waxes, pullulan, whey protein isolate, cellulose, alginates, corn zein, gluten, soy protein, caseinate, chitosan, pectin, carrageenan, xanthan gum, acacia (arabic) gum, agar, guar gum, modified starch, locust bean gum, konjac gum, and tara gum, or combinations thereof. The tablet may be a biconvex tablet having an upper convex surface (401, 501) and a lower convex surface (405, 505) connected by a cylindrical band (403, 503). The edible coating may comprise one or more herbs or spices.

The invention discloses a lactic acid bacteria microcapsule and a preparation method thereof, and relates to the technical field of lactic acid bacteria microcapsules, the preparation method comprises the following steps: adding a vinasse glutelin solution into pullulan for conjugation reaction, and freeze-drying to obtain a conjugate; adding cell wall powder, a conjugate, okra polysaccharide and a blackcurrant anthocyanin water extract, and carrying out ultrasonic dispersion to obtain an inner layer coating wall material liquid; adding lactic acid bacteria sludge into the inner-layer coating wall material liquid, uniformly stirring, homogenizing and carrying out ultrasonic treatment to obtain inner-layer coating emulsion; adding hydroxypropyl methyl cellulose phthalate and sodium alginate into deionized water, and adding a calcium chloride solution to obtain an outer layer wall material solution; and mixing the inner-layer coating emulsion with the outer-layer wall material solution, homogenizing at high pressure, drying, sealing in vacuum, keeping out of light, and storing in an environment of 4-5 DEG C to obtain the lactic acid bacteria microcapsule.

The invention discloses a method for predicting freezing resistance of wheat frozen dough based on a multiplex PCR molecular marker technology, and belongs to the technical field of molecular biology. According to the invention, a specific multiplex PCR system is constructed, and a high-molecular-weight glutenin subunit combination related to dough freezing resistance is synchronously identified. The method provided by the invention comprises the following steps: extracting wheat genome DNA by using an improved CTAB method; and carrying out synchronous amplification and identification on the subunits of the sites Glu-A1 and Glu-D1. According to the invention, efficient and rapid integrated detection of a plurality of high-quality subunits is realized, and reliable technical support is provided for directional breeding of wheat varieties with excellent frozen dough quality.