The invention discloses a method for extraction of maize yellow
pigment and
alcohol soluble
protein from maize
protein powder. The method includes: crushing the maize
protein powder, then conducting sieving by a 60-100 mesh
sieve, adding
ethanol with a material-
liquid ratio of 1:(8-16), conducting ultrasonic treatment at 200-600W for 10-50min by an
ultrasound assisted extraction technique; centrifuging the
raw material and the extraction
solvent, then collecting the supernatant and performing reduced pressure
distillation till an orange red oily liquid, and conducting
drying to obtain a
pigment product;
drying the centrifuged precipitate, adding
ethanol diluted to 60%-90% according to a material-
liquid ratio of 1:(6-14), conducting ultrasonic treatment under ultrasonic power of 200-400W for 10-50min, performing
centrifugation and taking the supernatant, adjusting isoelectric points, collecting
alcohol soluble protein from the precipitate, and conducting
drying to obtain the finished product. According to the invention, maize yellow
pigment and
alcohol soluble protein with high purity can be extracted in steps by one production process, the
solvent can be recycled, the production cost is saved, the extraction efficiency is improved, and the operation is simple, so that the method is easy to realize industrialization.