40results about "Plant zeins" patented technology

The present invention relates to a simplified extraction process for obtaining an extract rich in proteins from plant materials, and preferably rich in Zein proteins from plant materials such as corn (maize, Zea mays) plant materials, the corresponding protein extract, a process for preparing a stabilized protein extract solution, the corresponding, stabilized protein extract solution as such, and a process for the preparation of a cosmetic composition prepared based on the extraction, as well as cosmetic compositions as such comprising the extract or the stabilized protein extract solution of the present invention.

A method for modifying a gliadin and an application thereof are provided. The method includes the following steps: dissolving the gliadin in a solvent to obtain gliadin solution; and adding a proline endoprotease to the gliadin solution for a reaction, and adjusting the pH value of solution to neutral according to a gradient, to obtain a modified gliadin. The modified gliadin obtained by the present invention may be uniformly dispersed in water, and may still maintain a uniform dispersion state in the water after high-temperature thermal sterilization and long-term storage, or even in the presence of sodium chloride, it has good thermal stability, salt resistance, and storage stability.

Described herein is a corn protein product, comprising a first fraction comprising 75 wt % to 95 wt % (dry solids) protein and a second fraction comprising 60 wt % to 80 wt % (dry solids) protein, wherein the first fraction is a zein-enriched fraction and the second fraction is a zein-depleted fraction and a method of achieving the same. Further described herein is a corn protein product derived from destarched corn gluten meal comprising a first fraction comprising 78 wt % to 83 wt % (dry solids) protein and a second fraction comprising 70 wt % to 80 wt % (dry solids) protein, wherein the first fraction is a zein-enriched fraction and the second fraction is a zein-depleted fraction.

The invention discloses high-purity zein as well as an industrial preparation method and application thereof. The invention belongs to the technical field of corn deep processing. The method comprises the following steps: firstly, removing starch in the corn protein powder by using a high-temperature amylase technology, then removing fat and pigments in the corn protein powder by using a sodium carbonate aqueous solution with the pH value of 8-9, then obtaining a zein extracting solution by using a pipeline type continuous ultrasonic countercurrent extraction technology, and decolorizing by using activated carbon, concentrating and drying to obtain the zein. The zein prepared through the method is white solid powder, the purity reaches 98% or above, the zein can be applied to the food and medicine industry, starch enzymatic hydrolysate, fat and pigment obtained in the preparation process can be fully utilized, and the utilization efficiency of the corn protein powder is improved.

A method for modifying a gliadin and an application thereof are provided. The method includes the following steps: dissolving the gliadin in a solvent to obtain gliadin solution; and adding a proline endoprotease to the gliadin solution for a reaction, and adjusting the pH value of solution to neutral according to a gradient, to obtain a modified gliadin. The modified gliadin obtained by the present invention may be uniformly dispersed in water, and may still maintain a uniform dispersion state in the water after high-temperature thermal sterilization and long-term storage, or even in the presence of sodium chloride, it has good thermal stability, salt resistance, and storage stability.

The invention discloses a chemical-photodynamic therapy combined nano drug delivery system as well as a preparation method and application thereof. The preparation method comprises the following steps: synthesizing Zein-HPG from zein through anionic ring-opening polymerization reaction; then propiolic acid is combined with hydroxyl of the Zein-HPG through an esterification reaction, and a Zein-HPG-PA carrier is obtained; finally, curcumin is loaded to the Zein-HPG-PA carrier through a phenol-alkyne click reaction, and the nano drug delivery system Zein-HPG-PA (at) CUR is obtained. The nano drug delivery system disclosed by the invention has pH responsiveness, the loaded curcumin is released in a weak acid environment of tumors, and chemical / photodynamic combined treatment of tumors can be realized, so that a more ideal treatment effect is achieved.

The invention belongs to the technical field of agricultural product processing, and particularly relates to a zein separating device which comprises a supporting frame, a separating box is fixedly installed in the supporting frame, a plurality of liquid removing cylinders are arranged in the separating box, an annular plate is arranged between the liquid removing cylinders, and the two sides of the annular plate are slidably connected with the inner wall of the separating box. A flow cavity is formed between the outer wall of the annular plate and the inner wall of the separation box; when the liquid removing cylinder centrifugally rotates to separate a solution from impurities, a shaft plate is intermittently pushed to drive a plurality of poking rods to move towards a residue filtering plate in the liquid removing cylinder, a shifting rod slides in the liquid removing cylinder, and the poking rods move towards filtering holes in the residue filtering plate, so that the poking rods poke inner holes of the residue filtering plate, and the liquid removing cylinder is separated from the impurities. And the phenomenon that impurities block inner holes of the residue filtering plate when the impurities are separated from the solution, and flow separation operation of the solution in the liquid separation cylinder is affected is prevented, and the effect of dredging the filtering holes in the residue filtering plate when the solution and the impurities are separated is achieved.

The invention discloses a modified zein delivery carrier as well as a preparation method and application thereof. The modified zein delivery carrier is obtained by preparing zein nanoparticles with solid structures or hollow structures from a Na2CO3 solution or a Na2CO3 sacrificial template solution and then performing phosphorylation modification. Compared with nano-particles prepared from unmodified zein, the phosphorylated zein nano-particles have the advantages that the solubility is better, the storage stability is higher, the emulsifying property, the foaming property and the encapsulating property are remarkably improved, and the performance of the phosphorylated zein nano-particles serving as a delivery carrier is greatly improved.

The invention discloses a recombinant promoter and a method for improving the expression quantity of an exogenous gene in a plant seed, which are characterized in that a sequence for inhibiting the expression of a seed storage protein is added between the promoter and the exogenous gene, so that the expression of the storage protein in the seed is inhibited while the specific expression of an exogenous recombinant protein in the seed is realized; an environment more suitable for expression and accumulation of foreign proteins is created, the expression quantity of the foreign recombinant proteins in plant seeds is improved, and the cost of producing the recombinant proteins by plants is reduced.

The invention discloses colla corii asini collagen peptide modified zein animal and plant double-protein nanoparticles as well as a preparation method and application thereof in preparation of medicines for treating diabetes mellitus. The preparation method comprises the following steps: pretreating a colla corii asini material, performing ultrasonic assistance, extracting colla corii asini collagen by utilizing pepsin, preparing colla corii asini collagen peptide by utilizing a compound enzyme preparation, and preparing the double-protein nanoparticle CCAP-Z-Myr from the hydrophilic colla corii asini collagen peptide, hydrophobic zein and myricetin. The CCAP-Z-Myr has very high oxidation resistance and alpha-glucosidase inhibition activity, the alpha-glucosidase inhibition rate reaches up to 78.5%, the alpha-glucosidase inhibition rate of the CCAP-Z-Myr is equivalent to that of a common meal oral hypoglycemic drug acarbose in the range of 40-100 micrograms / mL, and the alpha-glucosidase inhibition rate of the CCAP-Z-Myr is remarkably higher than that of the acarbose when the CCAP-Z-Myr is 20 micrograms / mL. Therefore, the prepared compound has the hypoglycemic performance superior to that of acarbose at low concentration.

A water-soluble prolamin composition, such as a zein composition, and methods for producing the same. A method for tagging items comprising applying a plurality of non-coding DNA tags in a prolamin composition, such as a zein composition, wherein the selection of the particular taggants corresponds with a binary or nonbinary code sequence containing information about the tagged items.

The present inventors confirmed that when a brazzein expression recombinant vector for high expression of brazzein in Saccharomyces cerevisiae was prepared and a S. cerevisiae strain Y2805 was transformed with the recombinant vector, the expression level of brazzein was particularly high, thereby completing an optimal expression system for mass-producing brazzein. Further, when the brazzein expression system is cultured under the optimal culture conditions according to the present invention, the amount of brazzein produced is further increased, the purification process is simple, and costs are reduced. Therefore, it is expected that the brazzein expression system according to the present invention can be widely used for mass-producing and commercializing brazzein, which is a sweet protein.

The present invention discloses a milk protein-containing composition comprising at least one milk protein; at least a first protein; at least a second protein; and at least a linker comprising an amino acid sequence encoded by a nucleotide sequence that is at least 80% similar to a sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, and SEQ ID NO:21.

The invention relates to the technical field of food development, in particular to a corn peptide-pea peptide composite peptide and a preparation method and application thereof. The composite peptide provided by the invention consists of corn peptide and pea peptide, the mass ratio of the corn peptide to the pea peptide is (1-4): 1, and the content of Lys in the composite peptide is greater than 1.5 g / 100g protein. Compared with corn peptide, the composite peptide provided by the invention has remarkable advantages in the aspects of amino acid score, amino acid ratio coefficient, amino acid ratio coefficient score and the like; the bitterness value is obviously reduced, and the palatability is good; the compound disclosed by the invention has good anti-oxidation, anti-inflammatory and anti-apoptosis activities on a cellular level, has antagonistic and protective effects on H2O2-induced Caco-2 cell damage, can be applied to preparation of related foods, medicines and health-care products, and has good market application and popularization values.

Biocompatible and biodegradable composite material for the controlled underwater release of at least one active agent, wherein the composite material comprises at least one hydrophobic compound, at least one hydrophilic compound, at least one active agent. The at least one hydrophobic compound is zein, the at least one hydrophilic compound is polyvinylpyrrolidone (PVP), the at least one active agent is curcumin. The biocompatible and biodegradable composite material may be used for the underwater release of the active agent suitable for preventing and / or treating coral bleaching.

Genetically engineered bacteria, pharmaceutical compositions thereof, and methods of modulating and treating diseases associated with hyperphenylalaninemia are disclosed.

The present invention comprises a method, process, and composition that enhances the performance of paper straws through the use of zein treatments which significantly enhance the wet strength of paper straws. Selection of the paper substrate found that Hanji paper displays remarkable durability, reinforcing performance of the straws even when exposed to water and high salinity. Furthermore, the present invention exhibits substantial effectiveness in curbing starch migration from the paper straws, creating a protective barrier against starch-based glues that might otherwise compromise beverage flavor and pose gluten-related concerns. Additionally, the present invention outlines a method for crafting spirally-wound straws using a biodegradable paper strip impregnated with the zein treatment. The straws of the present invention offer an adhesive-free, sustainable, and environmentally-friendly solution for making durable straws.

The application belongs to the technical field of gene editing, and particularly relates to a recombinant expression vector, a recombinant bacillus subtilis and application thereof. The recombinant expression vector comprises an upstream homologous arm sequence, a downstream homologous arm sequence and a gene sequence coding high-sulfur zein for knocking out an endo-1, 4-beta-D-glucanase gene coding region. The application uses a homologous recombination method to induce expression of the Zein gene under control of an endo-1, 4-beta-D-glucanase promoter and a signal peptide sequence, and obtains a homologous recombination bacillus subtilis for efficiently secreting Zein protein. The Met content in fermentation liquor of the obtained homologous recombination bacillus subtilis can be increased by 94.18% compared with that of wild bacteria.

This invention discloses a method for cultivating high-lysine, high-quality protein maize. The method involves transferring an RNAi interference expression cassette that inhibits α-glucosinolate expression and a high-lysine fusion gene expression cassette into the maize genome to cultivate high-lysine, high-quality protein maize. The high-lysine fusion gene expression cassette includes a lysine-rich γ-glucosinolate fusion gene, the nucleotide sequence of which is shown in SEQ ID NO. 7. This invention, while inhibiting α-glucosinolate expression in maize, also specifically expresses a lysine-rich γ-glucosinolate fusion gene. The increased expression of γ-glucosinolate promotes the formation of the maize cuticle, resulting in high-quality, flint maize.

The application discloses a method for improving emulsifying performance of a zein graft, and the method comprises the following steps: adding anti-dextran monoclonal antibody D24 in a process of preparing a zein-dextran graft emulsion, and improving the emulsifying performance of the zein-dextran graft emulsion.

The invention is suitable for the technical field of protein vaccines, and provides Zera-3Ag protein nanoparticles for preventing and treating BVD and IBR as well as a preparation method and application of the Zera-3Ag protein nanoparticles, and the Zera-3Ag protein nanoparticles comprise E2-1b-Zeraa fusion protein, E2-1d-Zeraa fusion protein and gD-Zeraa fusion protein; the amino acid sequences of the E2-1b-Zera fusion protein, the E2-1d-Zera fusion protein and the gD-Zera fusion protein are respectively shown as SEQ ID NO: 2, SEQ ID NO: 4 and SEQ ID NO: 6 in a sequence table. After animals are immunized by the Zera-3Ag protein nano-particles, an effective immune protection effect can be generated, and the Zera-3Ag protein nano-particles have a good prevention and control application prospect on bovine viral diarrhea and bovine rhinotracheitis.

A method for extracting zein from a zein-containing material and applying it as a coating on paper material. The method involves batch or continuous extraction of zein from a zein-containing material by adding ethanol to the zein-containing material to form a zein-ethanol mixture; separating ethanol from zein in the zein-ethanol mixture by addition of water to form a separated zein-ethanol mixture; and utilizing the separated zein-ethanol mixture in a coating process with a paper material as a substrate.

The invention discloses a preparation method of high-purity zein, and relates to the technical field of corn protein powder processing, and the preparation method specifically comprises the following steps: pretreating corn protein powder, dissolving the pretreated corn protein powder in water, heating, carrying out primary enzymolysis by using acid protease, carrying out secondary enzymolysis by using a mixture of amylase and pullulanase, and carrying out secondary enzymolysis by using a mixture of amylase and pullulanase; finally, carrying out enzymolysis for three times by adopting cellulase; filtering the enzymatic hydrolysate, extracting filter residues for multiple times by using a mixture of acetone and water as an extraction solvent, and collecting the filter residues; and carrying out multi-stage purification on the collected filter residues, concentrating, filtering, and carrying out multi-stage drying to obtain the high-purity zein. According to the method, impurities are removed through staged enzymolysis, then an enzymolysis solution is extracted for multiple times by adopting a gradient concentration solvent, and multi-stage purification is carried out, so that the high-purity zein is prepared; the method is simple to operate and high in resource utilization rate.

The invention discloses a method for improving the expression quantity of foreign proteins in seeds, which comprises the following steps: inhibiting the expression of prolamin in plant seeds, and specifically expressing the foreign proteins to realize high expression and accumulation of the foreign proteins. According to the method, the expression quantity of the exogenous recombinant protein in the plant seeds can be greatly improved, the problem that the yield of the recombinant protein produced by utilizing the plant seeds in the existing method is low is solved, the application value and the economic value of a plant bioreactor are improved, and a new choice is provided for large-scale low-cost production of the recombinant protein in the seeds.

The application discloses a preparation method of polysaccharide gel-coated iron nanoscale enzyme and an alcohol-soluble protein modification application thereof, takes carrageenan as a gel matrix, disperses oxidized sodium alginate and histidine in the carrageenan gel, forms a dynamic Schiff base between aldehyde groups of the oxidized sodium alginate and amino groups of the histidine, and forms the iron nanoscale enzyme by complexing with imidazole groups and carboxyl groups in the gel system and in-situ mineralization, so that the preparation process is simple, the condition is mild, the cost is low, the obtained polysaccharide gel-coated iron nanoscale enzyme can modify the structure of zein based on the peroxidase-like activity of the iron nanoscale enzyme, grafts various phenolic compounds on the zein, improves the functional properties and nutritional value of the protein, and solves the problem that natural enzymes such as laccase, peroxidase and tyrosinase cannot catalyze the oxidative modification of the protein in an ethanol-water binary solvent and cannot play a catalytic role due to easy denaturation.

The invention discloses a method for extracting prolamin from corn gluten meal, and relates to the technical field of agricultural and sideline product processing. The method comprises the following steps: S1, adding a deep-eutectic solvent into the corn gluten meal according to the mass volume ratio of the corn gluten meal to the deep-eutectic solvent of 1g: (10-60) mL, and carrying out ultrasonic treatment for 30-120 minutes; and S2, centrifuging the ultrasonic solution, taking supernate, carrying out isoelectric point adjustment, standing, collecting precipitate, and freeze-drying the precipitate to obtain the zein powder. Compared with a traditional ethanol method, the method for extracting the prolamin in the corn gluten meal has the advantages that the eutectic solvent is green and environment-friendly, the extraction efficiency is high, and the cost is low; the extraction amount of the extracted prolamin reaches up to 171.04 mg / g, the protein purity reaches up to 84.23%, and both the extraction amount and the purity of the prolamin are obviously higher than those of a traditional ethanol method.

The invention discloses a corn peptide for repairing liver and a preparation method thereof, and belongs to the technical field of corn peptide extraction. The preparation method comprises the following steps: mixing choline chloride, lactic acid and polyhydric alcohols to obtain an extracting solution, mixing the extracting solution with corn flour, and performing microwave heating reflux reaction to obtain a zein extracting solution; performing enzymolysis on the zein extracting solution by using flavourzyme and alkaline protease to obtain enzymatic hydrolysate; the corn peptide is obtained by filtering, concentrating and dialyzing the enzymatic hydrolysate, small molecule peptide chains in the corn peptide are high in proportion and easy to absorb, the effect of the corn peptide can be brought into play, and the whole extraction process does not involve organic solvents and is environmentally friendly and efficient.

Disclosed herein are biopolymer-based coatings and products incorporating such coatings. Related methods and use are also provided.

The present invention discloses a modified zein delivery vehicle, its preparation method, and application. The modified zein delivery vehicle comprises solid or hollow zein nanoparticles prepared using a Na2CO3 solution or a Na2CO3 sacrificial template solution, followed by phosphorylation. Compared to nanoparticles prepared from unmodified zein, the phosphorylated zein nanoparticles exhibit improved solubility, greater storage stability, and significantly enhanced emulsification, foaming, and encapsulation properties, significantly enhancing their performance as a delivery vehicle.