Liquid enzyme agent for quick detection of residual pesticide toxicity and preparation method and using method thereof

A technology of pesticide residues and liquid enzymes, applied in biochemical equipment and methods, measurement/inspection of microorganisms, and analysis through chemical reactions of materials to achieve the effect of ensuring accuracy

Inactive Publication Date: 2006-02-08
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The liquid enzyme reagent for rapid detection of organophosphorus and carbamate pesticide residues of the present invention can be stored stably at room temperature at 20-25°C, thereb

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Put 30μl of acetylcholinesterase, 2mL of 0.1M Tris-HCl buffer solution with pH=7.8-8.2, and 5mg of sodium taurine into a beaker and stir well to make a liquid enzyme reagent; put 0.1mL of liquid enzyme reagent into 2mL Put it in a safe bottle, vacuum it and seal it; usually store it in a refrigerator at 4°C.

[0030] When rapid detection of organophosphorus and carbamate pesticide residues is required, the reagent package is prepared according to the above-mentioned method, and the detection is carried out by the enzyme-catalyzed kinetic photometry measurement with the pesticide residue rapid detector.

Embodiment 2

[0032] Put 100μl of butyrylcholinesterase, 10mL of 0.1M phosphate buffer solution with pH=7.8-8.2, and 20mg of sodium taurine into a beaker and stir evenly to make a liquid enzyme reagent; put 0.3mL of the liquid enzyme reagent into Put it in a 2mL ampoule bottle, vacuum it and seal it; usually store it in a refrigerator at 4°C.

[0033] When rapid detection of organophosphorus and carbamate pesticide residues is required, the reagent package is prepared according to the above-mentioned method, and the detection is carried out by enzyme-catalyzed kinetic photometry with a pesticide residue rapid tester

Embodiment 3

[0035] Put 50μl of acetylcholinesterase, 4mL of 0.1M Tris-HCl buffer solution with pH=7.8-8.2, and 8mg of sodium taurine into a beaker and stir evenly to make a liquid enzyme reagent; put 0.2mL of the liquid enzyme reagent into a 2mL safe Put it in a beaker bottle, vacuum it and seal it; usually store it in a refrigerator at 4°C.

[0036] When rapid detection of organophosphorus and carbamate pesticide residues is required, prepare a reagent pack according to the above method, and perform detection by color change.

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PUM

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Abstract

The invention provides a method for preparing a liquid enzyme reagent which is used to fast measure the organophosphorus and carbamate toxicity of pesticide residue and its using method. The agent is formed by choline esterase, buffer solution and stabilizer. The preparing method: 1) preparing the liquid enzyme reagent; 2) placing the liquid enzyme reagent into the On Bei bottle and sealing the port after vacating and preserving it inside the ice-box at 4 degree. The liquid enzyme reagent can be stored at indoor temperature above 15 days so that the activity loss is below 10%, which can fast measure the methylene oxide of the sea foods, the flour, the fried bean milk cream rolls and the dry fruit by matching the bottom object reagent, the color reagent and the buffer solution reagent.

Description

technical field [0001] The invention relates to a composition, in particular to a liquid composition reagent, in particular to an enzyme-containing liquid composition reagent for rapid detection of organophosphorus and carbamate pesticide residues. Background technique [0002] The harm of residual pesticides to human health is one of the hot spots in food safety issues. Worldwide, poisoning incidents caused by excessive residues of organophosphorus and carbamate pesticides have occurred frequently, of which 69.5% are caused by organophosphorus caused by pesticides. Therefore, it is very important to monitor pesticide residues in food and environment. At present, the enzyme inhibition method is usually used for the rapid detection of organophosphorus and carbamate pesticide residues, and the degree of enzyme inhibition is used to measure by enzyme-catalyzed kinetic photometry with the aid of a pesticide residue rapid tester, or by color change, or by acidity measurement an...

Claims

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Application Information

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IPC IPC(8): G01N21/78C12Q1/00
Inventor 邹明强王大宁张晓华邱月明王楠郭飞马
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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