120 results about "Uterine endometrium" patented technology

A method and composition for effecting chemical necrosis of a tissue lining of a mammalian body cavity, particularly a uterine endometrium, by delivering a caustic tissue necrosing composition, e.g., a silver nitrate and dextran paste, to the tissue to be necrosed and allowing the paste to remain in contact with the target tissue for a period of time sufficient to chemically necrose substantially the entirety of the tissue lining, and then contacting the caustic composition with a deactivating agent, e.g., an aqueous sodium chloride solution, thereby rendering the caustic composition non-caustic, and then rinsing the cavity. Compositions and methods for delivering medicaments are also disclosed.

The invention relates to a preparation method and application of collagen scaffold composite bone marrow-derived mesenchymal stem cells (BMSCs). The preparation method comprises the steps of preparing single cell suspension after trypsinizing the BMSCs, uniformly dropwise adding the single cell suspension to a collagen scaffold, putting the collagen scaffold into an incubator to be cultured, and adding an L-DMEM complete medium to continue culture, thus obtaining the collagen scaffold composite BMSCs. The preparation method has the advantages that the following defects are overcome: endometria are seriously injured as intrauterine adhesion is mechanically separated by adopting hysteroscopic surgery, intrauterine devices or anti-adhesion materials are put after the surgery and estrogens are given after the surgery to promote intima growth; the problem of intima scars can not be solved; functional intima repair can not be achieved; adhesion is very easy to happen again. As active ingredients for treating serious endometrium injury, the BMSCs are convenient to obtain, secrete growth factors to improve the local microenvironment and immunoregulation, have good biocompatibility, degradability and safety, promote scarred endometrium repair and increase the intima thickness and local blood vessel density.

The subject matter of the instant invention is pertinent to the field of treatment of hormone-dependent conditions. New compounds and methods for treating these conditions are disclosed. Embodiments of the instant invention disclose methods for treating endometriosis, dysmenorrhea, breast cancer, uterine fibroids and endometrial hyperproliferation.

The embodiments herein provide a biodegradable and biocompatible T-plate nano-composite implant with stem cells for treating and repairing broken bones, damaged tissues and torn ligaments. The implant comprises a polymeric matrix part comprising poly lactic glycolic acids (PLGA), a bioceramic part comprising hydroxyapatite (HAp) nanoparticles and an endometrial stem cell. The PLGA and HAp nanoparticles act as a matrix and reinforcing agents respectively. A method is provided for synthesizing the T-plate implant. The method comprises synthesizing hydroxyapatite (HAp) nanoparticles, poly lactic glycolic acids (PLGA) and HAp nano composite implant. The casting of the poly lactic glycolic acids (PLGA) and HAp nano composite are done in a mold to obtain a T-plate nano composite. An endometrial stem cell from an epithelial cell lining from uterus is isolated and cultured. The endometrial stem cells are implanted on the nano-composite implant.

This invention relates to semi-solid compositions and semi-solid pharmaceutical products for use in the photodynamic treatment (PDT) of cancer, pre-cancerous conditions and non-cancerous conditions in the female reproductive system, the anus and the penis, preferably for use in PDT of endometrial, cervical, vulvar, vaginal, anal and penile dysplasia and HPV infections of the uterus, cervix, the vulva, the vagina, the anus and the penis. The semi-solid compositions and pharmaceutical products comprise an active ingredient which is 5-aminolevulinic acid (5-ALA) or a precursor or derivative of 5-ALA or pharmaceutically acceptable salts thereof. The invention relates further to methods of PDT of cancer, pre-cancerous conditions and non-cancerous conditions in the female reproductive system, the anus and the penis, wherein said semi-solid compositions and pharmaceutical products are used.

The invention discloses a preparation method of stem cell composite exosome, and particularly relates to a preparation method of umbilical cord mesenchymal stem cell and endometrial stem cell composite exosome. The method comprises the following steps: S1, preparation of umbilical cord mesenchymal stem cell exosome; s2, preparation of endometrial stem cell exosome; and S3, preparation of the umbilical cord mesenchymal stem cell and endometrial stem cell composite exosome by mixing the umbilical cord mesenchymal stem cell exosome with the endometrial stem cell exosome, adding a protective agent, and performing low-temperature freeze drying treatment to obtain stem cell exosome freeze-dried powder. The umbilical cord mesenchymal stem cell and endometrial stem cell exosome composite growth factor prepared by the method disclosed by the invention has the advantages of no pain, no surgery, no operation and no side effect for solving the problem of female reproductive health, can be used athome in combination with hospital detection, and is safe and efficient.

The invention provides a traditional Chinese medicine for treating postnatal lochiorrhea and a preparation method thereof. The traditional Chinese medicine comprises the following raw medicinal materials: cornu cervi degelatinatum, dried rehamnnia root, angelica, rhizoma zingiberis, peach kernels, Ligusticum wallichii, pollen typhae, excrementum pteropi, radix paeoniae alba, safflower, radix codonopsitis, liquorice, burnt folium nelumbinis, rhubarb, hawthorn, rhizoma cyperi, herba patriniae, phaseolus calcaratus, leeches, Rhizoma curcumae, Astragalus membranaceus and radix achyranthis bidentatae. The traditional Chinese medicine has the advantages of quick effect, strong effect, short treatment course, high safety, no obvious toxic and side effects and simplicity in drug use and can be used for improving microcirculation, promoting shrinkage of uterine smooth muscles, helping regenerating of endometrium and fading of inflammations, automatically discharging residual placenta and foetal membranes in utero and clearing accumulation of a uterine cavity.

The invention discloses a sanitary towel inclusion agent, which consists of cyclodextrin, DP-300, menthol and nano magnesium oxide in a mass ratio of 1-2:0.3-0.8:3.5-7.5:2-6. When the sanitary towel inclusion agent is used in the production of sanitary towels and panty liners, the smell of menses due to blood oxidation and the peculiar smell caused by secreta of an endometrium and a sebaceous gland and sweat are removed by a method combining physical adsorption and chemical adsorption when consumers use the sanitary towels, simultaneously the bacteria growth in a female menstrual period is effectively inhibited, and the probability of vaginitis due to bacterial infection in the female menstrual period is greatly reduced; and as a plant freshener is added, discomfort feeling caused by high-temperature menses can be relieved when the consumers use the sanitary towels, and medicaments are controlled to be not volatilized during the storage of the sanitary towels.

The invention discloses a preparation method of a cellular composite endometrial repair gel. The method comprises the following steps of 1. extracting and culturing endometrial stem cells to obtain endometrial stem cells; 2, extracting and culturing mesenchymal stem cells to obtain the mesenchymal stem cells; 3, preparing temperature-sensitive chitosan hydrogel; and 4, preparing the cellular composite endometrial repair gel. The invention also discloses application of the cellular composite endometrial repair gel in treating intrauterine adhesion. The invention has the following advantages that 1, autologous stem cell transplantation basically eliminates the risk of rejection; 2, the autologous endometrial stem cells and the mesenchymal stem cells are subjected to composite transplantation, so that regeneration of endometrium, microvessels and mesenchyme is accelerated, and the endometrium repair time is obviously shortened; and 3, the temperature-sensitive gel can be formed after local injection, which can only only provide a three-dimensional retention space for the stem cells, but also prevent the adhesion of regenerated mucous membranes.

The invention provides a Chinese medicine for treating postpartum lochiorrhea and a preparation method thereof. The Chinese medicine comprises the following raw medicinal materials: verbena, motherwort, angelica rhizome, ligustici wallichii, red paeony root, sappanwood, ginseng, dragon's blood, rhizoma sparganii, curcuma zedoary, fried fruit of immature citron, artemisia anomala, herba lycopi, the root of fangji, ground beeltle, caulis spatholobi, the root of red-rooted salvia, endothelium corneum gigeriae galli, radix curcumae, elecampane, rhizoma corydalis, myrrh, resina toxicodendri and radix rubiae. The Chinese medicine provided by the invention has the advantages of fast acting, high function, short treatment course, high safety, no significant toxic and side effects and easy medication, can improve microcirculation and enhance the contraction of uterine smooth muscle, is beneficial for updating of endometrium and inflammation subsiding, and also can drive to exhaust the placenta and fetal membrane remained in uterus automatically so as to remove intrauterine stasis.

The invention relates to a Chinese herbal medicine preparation for curing the endometrial disease, the infertility and the habitual abortion. The Chinese herbal medicine preparation is prepared through decocting the following Chinese herbal medicines with water: angelica sinensis, astragalus membranaceus, rubus idaeus, ciliatenerve knotweed root, spiranthes sinensis ames, polygonum perfoliatum, kalimeris indica root, herba lycopi, achyranthes aspera, ginseng, rubus pluribracteatus, rubus parvifolius root, rhizoma polygonat, solanum lyratum, cyperus rotundus, cistanche, daughters red, crneate lespedeza, Chinese rose, galium aparine, achyranthes root, sagina subulata, gold rattan, crowndaisy chrysanthemum, herba leonuri, sargentgloryvine stem, semen cuscutae, semen euryales and the like. The Chinese herbal medicine preparation is beneficial for the recovery of the physical function and the organ function, has no any toxic or side effects, achieves more than 93% of effective rate in treating the female infertility and the habitual abortion, achieves more than 94% of effective rate in treating the endometritis and the pelvic inflammation, achieves more than 89.4% of effective rate in treating the cervical hypertrophy, the cervical erosion, the xeromenia, the inflammatory mass, the uterine fibroid, the ovarian cyst and other gynecological diseases, and achieves more than 94.1% of effective rate in treating sterility due to oligozoospermia and the necrospermia.

The invention relates to the technical field of medicines, and provides applications of uterine cavity fluid-derived exosome in preparation of drugs and adjuvant therapeutic agents for treating infertility related diseases. Through the verification of experiments, exosome realizes the treatment or relieving of endometrial growth disorders by promoting the increasing of the thickness of endometrium, enhancing the activity of endometrial cells, reducing the damages to intimal cells, promoting VEGF expression, maintaining the dryness of endometrial stromal cells and stimulating proliferation; andthrough the realizing of the treating or relieving of maternal-fetal immune tolerance disorder-related diseases by achieving immune tolerance effects, treating spontaneous abortion and enhancing T helper lymphocyte levels, a novel theoretical basis can be provided for infertility treatment. In addition, for both fertilized eggs being fertilized in vivo or fertilized in vitro, the exosome can effectively enhance developmental rates in multiples; and implantation rates and farrowing rates of in vitro fertilization transplantation can be increased as well, so that positive auxiliary effects on infertility treatment can be achieved.

The invention discloses a preparation method and application of a composite decellularized amniotic membrane of endometrial stem cells in the technical field related to physiological health care, including preparation of complete amniotic membrane tissue, preparation of a translucent amniotic membrane, immersion disinfection, decellularization treatment, preparation of a stem cell amniotic membrane culture system, preparation and cultivation of endometrial stem cell composite decellularized amniotic membrane and other processes as well as cryopreservation, thawing and application. The endometrial stem cell composite decellularized amniotic membrane prepared by the method is applied to the preparation of endometrial repair materials, is transplanted into the endometrial injury site for repair of endometrial damage and increase the thickness of the endometrium; the decellularized amniotic membrane completely removes cells and nucleic acids, and does not produce rejection reactions in thebody; through combination of menstrual blood stem cells or endometrium stem cells with the decellularized amniotic membrane, the cells are homologous to endometrial cells, and the repair effect is good; the use of hormones in repair is completely excluded to avoid interference with the internal secretion of a human body.

The invention relates to an artificial endometrium and its preparation and application and particularly relates to an artificial endometrium. The artificial endometrium comprises a biocompatible stent, endometrial stromal cells/epithelial cells and a mixed culture solution. Through a 3D bioprinting technology, the biocompatible stent and the endometrial stromal cells/epithelial cells are processedinto an artificial endometrium with bioactivity. The mixed culture solution comprises a medium and a biohormone. The biocompatible stent is prepared through a 3D printing method. The artificial endometrium can be used as a research tool for pathophysiological mechanisms aiming at artificial endometrial diseases, can be used as a screening model for regulating and affecting factors related to embryo implantation, and can be used as a research tool for preventing or treating directly or indirectly related endometrial lesions caused by any factor.

The invention relates to the preparation of acellular amniotic membrane carrier combined autologous endometrial stem cells. The isolating culture of autologous endometrial stem cells, the preparationand preservation of acellular amniotic membrane carriers and the preparation of acellular amniotic membrane carrier combined autologous endometrial stem cells are specifically included. The disadvantages of the occurrence of mechanical separation of intrauterine adhesions of hysteroscopic surgery in patients with moderate to severe intrauterine adhesions, the placing of intrauterine devices or anti-adhesion materials after operations, the endometrial functional repair which cannot be realized by giving estrogen to promote endometrial growth after the operations and the easy occurrence of re-adhesion can be overcome. The acellular amniotic membrane carriers have good anti-adhesion abilities, biocompatibility, biodegradability and safety; and as the autologous endometrial stem cells are taken as the active ingredients which can treat severe endometrial injury, growth factors can be secreted to improve local microenvironment and immunoregulation, so that the repair of damaged endometriumcan be promoted, and endometrial thickness and local vascular density can be increased.

The invention belongs to the technical field of medicine, and particularly discloses a composition for improving thin endometrium and a preparation method and application thereof. The composition is prepared from stem cell culture supernatant and vitamin E. After the composition for improving thin endometrium is used, expression of VEGF and integrin beta 3 in endometrium can be enhanced, the thickness of endometrium is increased, and uterus receptivity and blood supply are improved.

The invention discloses application of cyanidin-3-O-glucoside in preparation of a medicine for treating and/or preventing an endometrial hyperplasia disease. The cyanidin-3-O-glucoside is found to becapable of improving endometrial hyperplasia and endometrial hyperproliferation caused by Cd exposure, and the cyanidin-3-O-glucoside intervenes in the process of protecting growth and development ofendometrial cells by maintaining the estrogen effect level, so that the uterus can normally grow, embryo implantation is ensured, and meanwhile, the cyanidin-3-O-glucoside has a protection effect on the endometrium. Therefore, the cyanidin-3-O-glucoside has a good application prospect in preparation of the medicine for treating and/or preventing the endometrial hyperplasia disease, preparation ofthe medicine for treating and/ or preventing Cd-induced abnormal proliferation of the endometrial cells and preparation of the medicine for protecting the endometrium.

The invention discloses an automatic segmentation typing and thickness measuring method based on an endometrial ultrasound image, which comprises the following steps: 1) collecting an endometrial ultrasound scanning image set, manually selecting a standard uterus longitudinal cutting image, sketching an endometrial boundary, making a typing label, and establishing a training and testing sample set; 2) carrying out image preprocessing such as filtering on each image; removing noise influence and achieving an image enhancement effect; 3) respectively constructing a deep learning model for segmenting an endometrial region and typing, and respectively inputting a training set for training; 5) performing the same image preprocessing on the test set image, inputting the test set image into the trained deep learning model, and performing segmentation to obtain a segmented endometrial region and a typing result; 6) calculating the thickness of the endometrium by using a digital image morphological algorithm and a segmentation result of the endometrium ultrasonic image; by means of the method, the endometrial area can be accurately and effectively extracted, and more accurate thickness measurement and endometrial typing can be achieved.

The embodiment of the invention discloses an ultrasonic imaging method. The ultrasonic imaging method comprises: emitting ultrasonic waves to a uterus region of a to-be-detected object; receiving an ultrasonic echo returned from the uterus area of the to-be-detected object, and obtaining an ultrasonic echo signal based on the ultrasonic echo; processing the ultrasonic echo signal to obtain three-dimensional volume data of the uterus region of the to-be-detected object; acquiring position information of the endometrium in the three-dimensional volume data according to the image characteristicsof the key anatomical structure of the uterus region; based on the position information of the endometrium, optimizing endometrium imaging based on the three-dimensional volume data to obtain a targetultrasonic image of the endometrium; and displaying the target ultrasound image. The embodiment of the invention further discloses an ultrasonic imaging system, an ultrasonic image processing methodand an ultrasonic image processing system.

The invention discloses a novel degradable metal uterine cavity stent, a release system and a use method. The stent mainly adopts zinc and an alloy thereof, iron and an alloy thereof, and magnesium and an alloy thereof, has good strength and plasticity, can provide stable mechanical support in the service period, can be completely degraded after the service period, and does not need to be taken out through a secondary operation. Laser engraving is adopted for the stent, the thickness of the stent ranges from 50 to 150 [mu]m, and the coverage rate of the stent ranges from 10% to 20%. The shapeof the uterine cavity stent after being expanded conforms to a uterine cavity, and the expanded uterine cavity stent is 3-8cm in height, 2-5cm in width and 0.2-1cm in thickness. A slow-release medicine film on the surface of the stent contains epidermal growth factors, estrogens and antibiotic medicines, can accurately act on endometrium, reduces toxic and side effects of medicines, promotes growth of the endometrium and avoids uterine cavity infection. The release system is made of nylon or polyethylene glycol terephthalate and is free of biotoxicity, the release system can be removed after the uterine stent is expanded, no catheter is left, and bacterial infection caused by ascending from a lower genital tract is avoided. The stent and the release system can meet the use requirements ofthe human body uterine cavity stents.

The invention discloses a multifunctional cavitas uteri tissue collector. The multifunctional cavitas uteri tissue collector comprises a tube body and a curette head, wherein the curette head comprises a tubular curette body and a semispherical enclosure part; the front end of the curette body is enclosed by the enclosure part and the rear end of the curette body is communicated with the front end of the tube body; an included angle between the curette body and the tube body is 11 to 13 degrees; the curette body is provided with an attracting hole; a wedged scraping head is arranged on the hole wall, close to the enclosure part, of the attracting hole; a holding part is arranged at the rear end of the tube body and provided with a tube joint; an inner chamber of the holding part is respectively communicated with the tube body and the tube joint. According to the collector disclosed by the invention, functions of attracting and scraping of cavitas uteri tissues can be integrated, a satisfactory surgery effect can be achieved without needing operations for many times, and the surgical operation is relatively convenient; meanwhile, the injury to endometrium caused by artificial abortion surgery is reduced, and a minimally-invasive effect is achieved.