118 results about "Endostelium" patented technology

The invention discloses a kit and application thereof, the kit comprises a detection reagent for detecting the expression quantity of one or more RNA molecules, and accurate diagnosis of endometriosis can be realized. In addition, the invention also discloses a method for diagnosing the endometriosis.

An ultrasonic imaging device and a method for detecting endometrial peristalsis. The method includes: acquiring ultrasound echo data of the endometrium, and determining on said basis the peristaltic displacement or peristaltic speed of a point in the endometrium within a preset period of time; calculating peristalsis parameters of the endometrium according to the peristaltic displacement or the peristaltic speed of the point in the endometrium within the preset period of time, the peristalsis parameters being used to describe the moving state of endometrial peristalsis; and displaying the peristalsis parameters. Peristalsis parameters are calculated automatically without relying on the subjective determination of an ultrasound doctor, which improves the accuracy and efficiency of the detection of peristalsis.

A pharmaceutical composition according to an aspect can be applied to the prevention, treatment or the like of endometrial damage, Asherman's syndrome, or complications thereof by: reducing the level of collagen accumulation in the endometrium; increasing the expression level of VEGF; increasing the expression level of Ki67; increasing the expression level of Itgb3; increasing the expression level of Spp1; increasing the number of implantation sites; decreasing the expression levels of fibrosis-related factors; decreasing the expression levels of metabolism-related factors; increasing ATP production; and regulating the expression levels of mitochondrial function-related factors.

The invention discloses an endometrium sampler, which relates to the technical field of medical instruments and is technically characterized by comprising a protective sleeve, a movable tube, a brush rod, a top bead, bristles and a handle, the protective sleeve and the movable pipe are both of a hollow tubular structure, and the movable pipe is arranged in the protective sleeve and can move in the axial direction of the protective sleeve. The brush rod is arranged in the movable pipe, one end of the brush rod is fixedly connected with the handle, and the other end of the brush rod is fixedly connected with the top bead. The section, close to the top bead, of the brush rod is a brush rod front end; the bristles are attached to the front end of the brush rod and incline towards the top bead, and the top bead is of a semi-surrounding cover-shaped structure. During sampling operation, the handle is rotated to drive the front end of the brush rod to rotate, so that the bristles cover an inverted pear-shaped uterine cavity area; after the bristles finish sampling, the movable pipe is controlled to move towards the top bead, so that the bristles are folded and protected by the movable pipe; the sampling efficiency, the sample integrity and the diagnosis accuracy can be improved.

The invention relates to the technical field of biology, and particularly provides an endometrial organ culture method. By optimizing the endometrial organoid differentiation culture system, compared with an existing culture mode, the optimized culture system is easy to operate, the organoid with more complete physiological functions can be prepared without co-culture with stromal cells, continuous culture can be conducted for at least one year or above, and the obtained organoid is complete in secretion function and free of secondary pollution. The phenotype of cilia cells is more obvious.

The invention relates to the technical field of biomedicine, and discloses a method for constructing a mouse ovary endometriosis model, which comprises the following steps: identifying endometrial tissues of a same donor mouse and bilateral ovaries of a receptor mouse; constructing an ectopic transplantation model of the receptor mouse, and monitoring the survival states of the receptor mouse in different postoperative stages in real time; the growth condition of the ectopic endometrial tissue on the ovary surface is analyzed, and the tissue structure and pathological change of the ectopic endometrial tissue are recognized; determining a lesion area of the ectopic transplantation model, analyzing pathological characteristics and a formation mechanism of the lesion area, and analyzing a correlation degree between the ectopic transplantation model and human endometriosis; and analyzing the simulation effect of the ectopic transplantation model on human endometriosis, and constructing the ovarian endometriosis model of the receptor mouse based on the modeling effect and the ectopic transplantation model. The model forming rate of the endometriosis model and the accuracy of the research result can be improved.

Methods for enhancing the visualization of a myoma relative to the uterus and endometrium in ultrasound imaging are provided. The method includes receiving, by at least one processor, an ultrasound volume including ultrasound image data of a region of interest, automatically segmenting the uterus and endometrium in the ultrasound volume, segmenting one or more myomas in the ultrasound image data, and outputting the one or more myomas. A classification of each of the one or more myomas is generated based on a position of each of the one or more myomas relative to the endometrium and the uterus such that the display system presents at least one image identifying the segmented uterus, endometrium, and one or more myomas. The application also provides a system for enhancing the visualization of a myoma relative to the uterus and endometrium during ultrasound imaging.

The invention provides a biomarker for evaluating endometrial aplastic, a product and application, and belongs to the technical field of biology. Wherein the biomarker is microRNA-21-5p (micro Ribonucleic Acid-21-5p). The biomarker for evaluating endometrial aplastic provided by the invention has the advantages that the expression quantity of the biomarker in the endometrium of a normal pregnant woman and the expression quantity of the endometrium of a thin endometrium (TE) patient are obviously different; and an efficient, rapid, high-specificity and high-sensitivity detection and evaluation means can be provided for early discovery and diagnosis of TE patients, targeted intervention and embryo transplantation guidance.

The present invention relates to a conjugate comprising a FAP targeting ligand and an effector for use in the treatment and / or diagnosis of endometriosis, as well as associated methods and kits.

A device, system and methods for the non-thermal treatment of endometriosis.

An ultrasonic imaging method and device and a computer-readable storage medium are provided. The ultrasonic imaging method includes: ultrasonic waves are transmitted to a uterine region of an object to be detected; ultrasonic echoes are received based on the ultrasonic waves reflected from the uterine region of said object, and ultrasonic echo signals are acquired on the basis of the ultrasonic echoes; the ultrasonic echo signals are processed to obtain three-dimensional data of the uterine region of said object; the endometrium is identified from the three-dimensional data of the uterine region according to endometrium image characteristics of the uterine region to obtain position information of the identified endometrium; according to the position information of the identified endometrium, the cross section of the identified endometrium is imaged on the basis of the three-dimensional data to obtain an cross-sectional endometrium image; and the cross-sectional endometrium image is displayed.

An endoprosthesis delivery system includes a branched endoprosthesis having a long leg and a short leg, each leg having an end, the short leg including a tail that extends beyond the end of the short leg, a constraint attached to at least a portion of the long leg prior to deployment. The constraint also retains at least a portion of the tail. The constraint is configured to release both the long leg and the tail when the branched endoprosthesis is fully deployed.

The present invention relates to a device suitable for introducing a fertilized egg or embryo into a maternal endometrium. The device includes an elongate body including a distal tip and a cavity extending through the distal tip and to an aperture of the distal tip. The cavity is configured to receive and expel fertilized eggs or embryos through the aperture. The plunger is configured to be slidably received within the cavity. The distal tip includes a piercing portion for piercing a maternal endometrium, and the aperture is concave.

The present invention provides a method of determining endometrial status using a sample (e.g., a plasma sample) from a subject, comprising: (a) performing an assay on a blood sample from a subject to determine a miRNA expression profile wherein the miRNA expression profile comprises a plurality of expression levels of miRNAs, and (b) analyzing the miRNA expression profile using a computer-based machine learning model to obtain a predicted score.

The utility model provides a painless endometrium sampler, which relates to the technical field of sampler, and comprises an outer sleeve and a sleeve core rod, one end of the outer sleeve is provided with a handle tube, one end of the sleeve core rod is provided with a handle plate, the sleeve core rod penetrates through the handle tube and is inserted into the outer sleeve, one end of the sleeve core rod is provided with a piston part, and the piston part is provided with a handle plate. The outer side of the outer sleeve is coated with a flexible rubber hose, a through hole is formed in one end of the outer side of the flexible rubber hose, and a sampling hole corresponding to the through hole is formed in one end of the outer side of the outer sleeve; an air bag is arranged at one end of the flexible rubber hose; the outer side of the outer sleeve is coated with the flexible rubber hose, the outer side is in soft contact with a sampling part during insertion, foreign body sensation is reduced, the pressing ball can be pressed before insertion, air is inflated into the air bag through the suction pipe and the guide pipe, the size of the air bag can be conveniently changed according to actual insertion requirements, and in the insertion and sampling process, the air bag is not prone to falling off. The air bag at the front end softly extends into a sampling part and is contacted with a blunt surface, so that no pain is caused and the use is more comfortable.

The invention relates to the technical field of medicine, and particularly discloses an endometriosis prescription for dysmenorrhea with endometriosis. Comprising the following raw materials: 10-15 parts of radix salviae miltiorrhizae, 7-10 parts of rhizoma curcumae, 2-3 parts of flos carthami, 7-10 parts of vinegar turtle shell, 10-15 parts of salted radix achyranthis bidentatae, 7-10 parts of rhizoma corydalis, 10-15 parts of prepared fingered citron, 5-8 parts of radix bupleuri, 10-15 parts of radix paeoniae alba, 10-15 parts of caulis spatholobi, 10-15 parts of poria cocos, 7-10 parts of herba taxilli, 7-10 parts of radix dipsaci, 3-5 parts of fructus amomi, 7-10 parts of endothelium corneum gigeriae galli, 10-15 parts of hawthorn and 7-10 parts of liquorice. The agreement prescription of the internal different prescription has obvious curative effects on improvement of dysmenorrhea symptoms of EMs patients, reduction of tentacle nodules and appendage masses, improvement of pelvic cavity states, increase of pregnancy rates and the like, and is safe and free of obvious toxic and side effects.

The application discloses a mesenchymal stem cell preparation and application thereof in thin endometrium repair, and comprises the following steps: adding Enterococcus durans CGMCC 1.3249 and Bacillus subtilis CGMCC 1.15792 supernatant as effective components into cultured mesenchymal stem cells; and the stem cell preparation obtained by the method can effectively restore the endometrial thickness of mice, increase the number of glands, and improve the pregnancy rate of the thin endometrium mice.

The present invention relates to the field of biomedical technologies and discloses a method for constructing a mouse model of ovarian endometriosis. The method includes: identifying the endometrial tissue of a syngeneic donor mouse and the bilateral ovaries of a recipient mouse; constructing an ectopic transplantation model of the recipient mouse and real-time monitoring the survival status of the recipient mouse at different postoperative stages; analyzing the growth of the ectopic endometrial tissue on the ovarian surface and identifying the tissue structure and pathological changes of the ectopic endometrial tissue; determining the lesion area of the ectopic transplantation model, analyzing the pathological characteristics and formation mechanism of the lesion area, and analyzing the degree of association between the ectopic transplantation model and human endometriosis; analyzing the simulation effect of the ectopic transplantation model on human endometriosis, and constructing a mouse model of ovarian endometriosis for the recipient mouse based on the modeling effect and the ectopic transplantation model. The present invention can improve the modeling rate of the endometriosis model and the accuracy of research results.

Provided herein is a targeting molecule, wherein the targeting molecule binds to a surface molecule on an endometrial endothelial cell (EEC) in which expression of the surface molecule is upregulated.

The invention discloses an application of miR-2355-3p in endometriosis. The research discovers that the RNA transcriptional level of miR-2355-3p in an ectopic endometrial tissue sample is obviously reduced for the first time; the mimics RNA overexpressed by miR-2355-3p is cloned to a lentiviral vector, lentiviral packaging is carried out, the miR-2355-3p mimic lentivirus is obtained, then the miR-2355-3p mimic lentivirus is used for transfecting ectopic endometrial interstitial cells, proliferation of the ectopic endometrial interstitial cells can be inhibited, apoptosis of the ectopic endometrial interstitial cells can be promoted, growth of ectopic endometrial tissues is inhibited, and the mimic RNA-mimic lentivirus can be used for treating the endometrial interstitial cells. And a new possibility is provided for developing the medicine for treating the endometriosis.

The present invention relates to drospirenone for use in a method for treating endometriosis, endometriosis associated pelvic pain (EAPP) and / or dysmenorrhea comprising administering drospirenone in a biphasic regimen. The present invention further relates to the use of drospirenone administered in such a biphasic regimen as a contraceptive and for inducing amenorrhea, as well as to pharmaceutical compositions and kits comprising drospirenone administered in such a biphasic regimen.

The invention belongs to the field of biological materials, and particularly relates to a gel for repairing endometrial injury as well as a preparation method and application of the gel. The invention provides a gel for repairing endometrial injury. The gel is prepared from recombinant I-type humanized collagen, antibacterial peptide and mesenchymal stem cells. The gel provided by the invention can promote proliferation of endometrial cells, repair an endometrial microenvironment, promote regeneration of endometrium and effectively repair damaged endometrium, and has a development prospect in clinical application.

Methods for treating and non-invasively detecting endometriosis using menstrual discharge are provided.

The invention provides application of endometrial mesenchymal stem cell exosomes in atherosclerosis, and belongs to the technical field of biological medicine. The invention provides a bioactive preparation which is derived from specific tissues, has good safety and definite treatment directivity, can effectively intervene in the progress of atherosclerotic plaques and relieve pathological changes of blood vessels, and the treatment convenience and patient tolerance are remarkably improved through a non-invasive administration mode of the bioactive preparation. A brand new solution with excellent application potential is provided for clinical management of atherosclerosis.

The application discloses a preparation method of a 3D model of a maternal-fetal interface constructed by an endometrial organoid in vitro, and the method comprises the following steps: establishing a primary endometrial epithelial organoid and an endometrial stromal cell line in vitro; improving an extracellular matrix for assembling endometrial gland-like organoids and stromal cells; the constructed endometrial organoid model comprises gland cells and stromal cells; the endometrial organoid model is cultured by using a gas-liquid interface, so that the endometrial organoid model has a cavity epithelium and a gland structure; the endometrial organoid model simulates a menstrual cycle of the endometrium and performs function identification. The endometrial organoid model has the cavity epithelium and the gland epithelium for the first time, the tissue structure, the cell composition, the hormone-induced change, the gene expression characteristics of the organoid are similar to those in the body, and the organoid can reproduce the receptivity genes and the dynamicity of cilia which are necessary for embryo implantation in the body; the organoid can be used as a model for studying embryo implantation regulation, endometrial diseases and regeneration mechanisms.

Described herein are improved methods for the detection of endometriosis. Generally, the methods include, but are not limited to, applying machine learning algorithm to miRNA levels in order to detect, predict, diagnose, or monitor the presence or absence of endometriosis.

The invention discloses a drug-loaded gel and an application thereof in preparation of a drug for repairing endometrial injury, and belongs to the field of biological medicine, the drug-loaded gel is composed of poloxamer P407 and S100A8, the drug-loaded gel is prepared from PBS, and the ratio of the poloxamer 407 to the S100A8 to the PBS is 0.2 g: 5 [mu] g: 1 mL. According to the invention, poloxamer 407 hydrogel is used for carrying S100A8 to prepare a drug-loaded gel, and by constructing a rat endometrial injury model and applying the drug-loaded gel for treatment, the result shows that the drug-loaded gel can maintain the intact degree of endometrial epithelium and increase the levels of molecular indexes such as Ki-67, Claudin-1 and ZO-1 related to endometrial epithelium repair, so that the drug-loaded gel can be used for treating endometrial epithelium. Therefore, the endometrial injury of the rat is remarkably relieved, and a new idea is provided for repairing the endometrial injury.

The invention belongs to the technical field of natural products and pharmaceutical industry, and provides a compound for improving endometrial aging. The compound is ganoderic acid C2 and is screened by the following method: inducing an endometrial organ to form an aging endometrial model by using D-galactose; contacting a compound to be detected with the senescence endometrial model, and detecting the proportion of beta-galactosidase and / or EdU positive cells; and screening a compound capable of remarkably reducing beta-galactosidase and / or remarkably improving the proportion of EdU positive cells, namely the compound for improving endometrial aging. The model constructed by the invention can fully simulate the physiological status and pathological change of the senescent endometrium, the screening reliability is high, the period is short, the ethical problem is avoided, and a reliable method is provided for subsequent screening of natural products.

The application belongs to the technical field of biotechnology and medicine, and specifically discloses a single-domain antibody-exosome compound (Nb-EMX2-Exos) targeting EMX2 as well as a preparation method and application thereof. The compound is prepared by covalently coupling a single-domain antibody (Nb-EMX2) specifically binding to EMX2 protein to the surface of endometrial stem cell-derived exosomes (ESC-Exos). In-vitro experiments show that the compound can specifically recognize and efficiently internalize into damaged endometrial stromal cells with high expression of EMX2, and significantly promote the migration and proliferation of the cells. In-vivo experiments further prove that the compound can precisely target and enrich in damaged endometrial tissue, effectively promote the structural repair (thicken the endometrium and reduce fibrosis) of the endometrium, and completely restore the fertility function. The application provides a novel targeted treatment preparation for solving the problems of poor targeting of natural exosomes and limited treatment effect, and has important application value in the treatment of endometrial injury repair and related infertility.