The invention provides an endometrial polyp methylation marker combination for the first time. The endometrial polyp methylation marker combination comprises the following gene markers: IGF1R, CTBP1, TCF7L1, E2F3, CACNA2D4, KCNJ12, TPO, UGT1A8, UGT1A10, CABP5, CST9, ITGA2, DLGAP2, ESPNP, NBPF25P, RASA3, ZIM2, PXDN, HDAC4 and VAV2; the endometrial polyp can be effectively diagnosed by detecting the methylation level of the gene markers in a sample to be detected from a subject, the specificity reaches 87.27%, and the sensitivity reaches 91.11%.