14 results about "Endostelium" patented technology

The application discloses a mesenchymal stem cell preparation and application thereof in thin endometrium repair, and comprises the following steps: adding Enterococcus durans CGMCC 1.3249 and Bacillus subtilis CGMCC 1.15792 supernatant as effective components into cultured mesenchymal stem cells; and the stem cell preparation obtained by the method can effectively restore the endometrial thickness of mice, increase the number of glands, and improve the pregnancy rate of the thin endometrium mice.

Methods for treating and non-invasively detecting endometriosis using menstrual discharge are provided.

The application belongs to the technical field of biotechnology and medicine, and specifically discloses a single-domain antibody-exosome compound (Nb-EMX2-Exos) targeting EMX2 as well as a preparation method and application thereof. The compound is prepared by covalently coupling a single-domain antibody (Nb-EMX2) specifically binding to EMX2 protein to the surface of endometrial stem cell-derived exosomes (ESC-Exos). In-vitro experiments show that the compound can specifically recognize and efficiently internalize into damaged endometrial stromal cells with high expression of EMX2, and significantly promote the migration and proliferation of the cells. In-vivo experiments further prove that the compound can precisely target and enrich in damaged endometrial tissue, effectively promote the structural repair (thicken the endometrium and reduce fibrosis) of the endometrium, and completely restore the fertility function. The application provides a novel targeted treatment preparation for solving the problems of poor targeting of natural exosomes and limited treatment effect, and has important application value in the treatment of endometrial injury repair and related infertility.

This invention provides a formulation capable of promoting normal decidualization of the endometrium. An endometrial decidualization promoter or improver containing an effective amount of pyrroloquinoline quinone or its salt is prepared. Furthermore, a cell fusion promoter or improver containing an effective amount of pyrroloquinoline quinone or its salt is also prepared.

The application discloses an endometrium-targeted mitochondrial function compensation system and application thereof. The endometrium-targeted mitochondrial function compensation system comprises a function unit, a delivery unit and a control unit. The function unit comprises a vesicle preparation formed by wrapping donor mitochondria with red blood cell membranes. The donor mitochondria are isolated mitochondria with complete inner and outer mitochondrial membranes and ridge structures or engineered mitochondria carrying exogenous mitochondrial DNA. The application first constructs a comprehensive system integrating biological active ingredients, targeted delivery and intelligent control, can directly and efficiently repair the mitochondrial function of endometrial cells, promote endometrial proliferation, and provides an innovative solution for treating related reproductive diseases. The system has complete design, clear effect and good clinical transformation prospect.

The application discloses a kind of endometrial carcinoma molecular typing methods based on cascaded multiple instance learning, method is by the binary mask of tumor area generated to endometrial carcinoma whole section image preprocessing, to extract the image block in region of interest and construct instance set by dyeing normalization processing;Using PatchVMamba instance encoder, the feature of each image block in instance set is extracted to obtain the instance feature vector of each image block, and the instance feature bag of each whole section image is formed;Using attention mechanism, the instance feature bag is weighted and aggregated to obtain the slice-level feature representation;The slice-level feature is input into the three binary classifiers arranged in cascade to obtain the probability value corresponding to the three subtypes of endometrial carcinoma respectively, and a unified threshold is used for cascade judgment of the subtype classification of endometrial carcinoma to output the final typing result.The cascaded structure of the application makes the consistency of the typing result and the diagnosis of pathological experts high, and realizes accurate typing.

The present invention relates to an organ-on-a-chip device for mimicking the bone marrow microenvironment (1) comprising: a perivascular region (2) representing the perivascular niche; a central region (3) representing the central niche; an endosteal region (4) representing the endosteal niche; at least one fluid inlet channel (5); at least one channel for injecting cells, fluids and hydrogels (6) for each region (2, 3, 4); at least one containment port (7) to allow independent filling of the at least one channel for injecting cells, fluids and hydrogels (6); and at least one fluid outlet channel (8). The present invention also relates to a method for producing the device moulds using additive manufacturing, a method for producing the device from PDMS using soft lithography, a method for filling the device with hydrogel and performing three-dimensional culture, and use of the device.

This invention relates to the field of biotechnology, specifically, to a method for culturing endometrial organoids. By optimizing the endometrial organoid differentiation and culture system, compared to existing methods, the optimized system is simple to operate, does not require co-culturing with stromal cells to prepare organoids with more complete physiological functions, and can be cultured continuously for at least one year. The resulting organoids exhibit complete secretory function and a more pronounced ciliated cell phenotype.

The present application relates to the technical field of molecular diagnosis, and specifically relates to application of female uterine cavity flora as a marker in endometriosis diagnosis. The present application collects uterine cavity flora of patients, extracts genomic DNA, performs bacterial 16s-rRNA sequencing, uses KEGG as a reference database for function prediction, determines that abnormal abundance of uterine cavity flora dominant bacteria has significant correlation with dysmenorrhea and high CA125 value of endometriosis patients, clarifies the correlation between abnormal uterine cavity flora and endometriosis, and provides a new method for evaluating the risk of endometriosis by detecting uterine cavity flora atlas.

This invention pertains to the clinical application of traditional Chinese medicine, primarily focusing on the application of a Chinese herbal extract in the prevention and treatment of endometriosis. The invention also relates to a method for preparing this extract. The preparation method involves: taking 250 g of raw Curcuma zedoaria slices and heating them under reflux with 15 times the volume of 90% ethanol, extracting twice, 1.5 h each time; then extracting the residue with 10 times the volume of distilled water for 1.5 h; combining the ethanol and water extracts, filtering, and concentrating under reduced pressure at 55°C to a crude drug content of 1.93 g / mL. In vivo experiments have demonstrated that the extract significantly reduces the volume of ectopic endometrium. HE staining in nude mice in the endometrial lesion group revealed extensive inflammatory infiltration and angiogenesis in the ectopic endometrial lesions. Administration of the extract significantly reduced inflammatory infiltration and angiogenesis. Immunohistochemical staining of nude mice in the EMs group revealed high positive rates of PCNA and MVD cells. After administration of the herbal extract of this invention, the positive rates of PCNA and MVD cells were significantly reduced, demonstrating a significant anti-endometriosis effect.

Provided herein are methods of assessing whether a subject has endometriosis or is predisposed to endometriosis based on microbiome-based biomarkers in a sample of the subject. Kits useful for performing such assessments are also provided. Methods of treating or preventing endometriosis guided by information obtained from methods disclosed herein are also provided.

The application provides a method for judging endometrial receptivity. The method comprises the following steps: extracting total RNA of endometrium related tissue sample; reverse transcribing the total RNA of the sample into cDNA and amplifying and constructing a library; performing high-throughput sequencing on the library to obtain transcriptome data, analyzing and obtaining the expression level of genes related to endometrial receptivity; inputting the expression level of the related genes into an endometrial receptivity prediction model, and judging the endometrial receptivity according to the calculation result. The method can quickly and accurately judge the endometrial receptivity, and predict the endometrium related tissue of the subject suitable for the implantation window period of embryo transplantation.

This specification provides a butterfly six-step resection system and device for deep-invasive endometriosis, relating to the field of intelligent medicine. It includes: an anatomical relationship positioning module configured to locate the target operating area for the subject; a pelvic adhesion separation module configured to separate adhesions in the rectouterine pouch within the target operating area; a ureteral repositioning module configured to gradually expose and reposition the bilateral ureters by opening the peritoneum at the first extraperitoneal distance of the affected lateral peritoneum within the target operating area; and modules for resection of sacral ligament lesions, rectouterine pouch lesions, and rectal surface lesions. This application reduces the impact of individual surgeon experience differences on surgical outcomes through standardized procedures, achieving homogenized diagnostic and treatment quality across different levels of hospitals and surgeons with varying experience.