42 results about "Endostelium" patented technology

The present invention relates to a conjugate comprising a FAP targeting ligand and an effector for use in the treatment and / or diagnosis of endometriosis, as well as associated methods and kits.

The present invention provides a method of determining endometrial status using a sample (e.g., a plasma sample) from a subject, comprising: (a) performing an assay on a blood sample from a subject to determine a miRNA expression profile wherein the miRNA expression profile comprises a plurality of expression levels of miRNAs, and (b) analyzing the miRNA expression profile using a computer-based machine learning model to obtain a predicted score.

The application discloses a mesenchymal stem cell preparation and application thereof in thin endometrium repair, and comprises the following steps: adding Enterococcus durans CGMCC 1.3249 and Bacillus subtilis CGMCC 1.15792 supernatant as effective components into cultured mesenchymal stem cells; and the stem cell preparation obtained by the method can effectively restore the endometrial thickness of mice, increase the number of glands, and improve the pregnancy rate of the thin endometrium mice.

Provided herein is a targeting molecule, wherein the targeting molecule binds to a surface molecule on an endometrial endothelial cell (EEC) in which expression of the surface molecule is upregulated.

The invention discloses an application of miR-2355-3p in endometriosis. The research discovers that the RNA transcriptional level of miR-2355-3p in an ectopic endometrial tissue sample is obviously reduced for the first time; the mimics RNA overexpressed by miR-2355-3p is cloned to a lentiviral vector, lentiviral packaging is carried out, the miR-2355-3p mimic lentivirus is obtained, then the miR-2355-3p mimic lentivirus is used for transfecting ectopic endometrial interstitial cells, proliferation of the ectopic endometrial interstitial cells can be inhibited, apoptosis of the ectopic endometrial interstitial cells can be promoted, growth of ectopic endometrial tissues is inhibited, and the mimic RNA-mimic lentivirus can be used for treating the endometrial interstitial cells. And a new possibility is provided for developing the medicine for treating the endometriosis.

The present invention relates to drospirenone for use in a method for treating endometriosis, endometriosis associated pelvic pain (EAPP) and / or dysmenorrhea comprising administering drospirenone in a biphasic regimen. The present invention further relates to the use of drospirenone administered in such a biphasic regimen as a contraceptive and for inducing amenorrhea, as well as to pharmaceutical compositions and kits comprising drospirenone administered in such a biphasic regimen.

Methods for treating and non-invasively detecting endometriosis using menstrual discharge are provided.

The invention provides application of endometrial mesenchymal stem cell exosomes in atherosclerosis, and belongs to the technical field of biological medicine. The invention provides a bioactive preparation which is derived from specific tissues, has good safety and definite treatment directivity, can effectively intervene in the progress of atherosclerotic plaques and relieve pathological changes of blood vessels, and the treatment convenience and patient tolerance are remarkably improved through a non-invasive administration mode of the bioactive preparation. A brand new solution with excellent application potential is provided for clinical management of atherosclerosis.

The application discloses a preparation method of a 3D model of a maternal-fetal interface constructed by an endometrial organoid in vitro, and the method comprises the following steps: establishing a primary endometrial epithelial organoid and an endometrial stromal cell line in vitro; improving an extracellular matrix for assembling endometrial gland-like organoids and stromal cells; the constructed endometrial organoid model comprises gland cells and stromal cells; the endometrial organoid model is cultured by using a gas-liquid interface, so that the endometrial organoid model has a cavity epithelium and a gland structure; the endometrial organoid model simulates a menstrual cycle of the endometrium and performs function identification. The endometrial organoid model has the cavity epithelium and the gland epithelium for the first time, the tissue structure, the cell composition, the hormone-induced change, the gene expression characteristics of the organoid are similar to those in the body, and the organoid can reproduce the receptivity genes and the dynamicity of cilia which are necessary for embryo implantation in the body; the organoid can be used as a model for studying embryo implantation regulation, endometrial diseases and regeneration mechanisms.

Described herein are improved methods for the detection of endometriosis. Generally, the methods include, but are not limited to, applying machine learning algorithm to miRNA levels in order to detect, predict, diagnose, or monitor the presence or absence of endometriosis.

The application belongs to the technical field of biotechnology and medicine, and specifically discloses a single-domain antibody-exosome compound (Nb-EMX2-Exos) targeting EMX2 as well as a preparation method and application thereof. The compound is prepared by covalently coupling a single-domain antibody (Nb-EMX2) specifically binding to EMX2 protein to the surface of endometrial stem cell-derived exosomes (ESC-Exos). In-vitro experiments show that the compound can specifically recognize and efficiently internalize into damaged endometrial stromal cells with high expression of EMX2, and significantly promote the migration and proliferation of the cells. In-vivo experiments further prove that the compound can precisely target and enrich in damaged endometrial tissue, effectively promote the structural repair (thicken the endometrium and reduce fibrosis) of the endometrium, and completely restore the fertility function. The application provides a novel targeted treatment preparation for solving the problems of poor targeting of natural exosomes and limited treatment effect, and has important application value in the treatment of endometrial injury repair and related infertility.

The present invention relates to a composition for endometrial regeneration containing a uterus-derived decellularized extracellular matrix (UdECM) and a manufacturing method therefor. Characterized by containing uterus-derived decellularized extracellular matrix (UdECM), the composition can exhibit the effect of enhancing the regeneration, receptivity, and implantation capabilities of the endometrium.

The invention discloses a novel application of a compound beta-alanine (beta-alanine) in preparation of a medicine for improving oocyte quality and treating ovarian endometriosis (OEM) related infertility, and belongs to the technical field of biological medicines. An OEM animal model proves that beta-alanine can remarkably improve reproductive function disorder of model animals, specifically, follicular dysplasia caused by endometriosis is effectively relieved, the fertility is improved, and the litter size is increased; in the aspect of oocyte quality, the compound can promote normal discharge of a first polar body in an oocyte in-vitro maturation process, reduce cytoplasm fragmentation, recover spindle body form and microtubule arrangement, enhance mitochondrial membrane potential stability and maintain the integrity of an ultrastructure. The results show that beta-alanine can reverse oocyte quality reduction caused by ovarian endometriosis in multiple dimensions, has a definite treatment potential, and provides an important candidate molecule for developing a novel assisted reproduction intervention strategy for targeting oocyte health.

This invention provides the application of a uterine-derived Sphingomonas DS-28 strain and its metabolites in the treatment of endometritis, relating to the field of microbial technology. The Sphingomonas DS-28 strain provided by this invention possesses uterine colonization ability, good niche adaptability, and significant efficacy in treating Escherichia coli-induced endometritis. Animal experiments show that the preparation of this strain can effectively alleviate uterine redness and swelling, reduce pathological damage, and significantly reduce [the condition / progression]. TNF-alpha , IL-6 , IL-8 It inhibits gene expression and suppresses the NF-κB signaling pathway, exhibiting superior anti-inflammatory and tissue repair effects compared to other Sphingomonas strains C3-25.1, C3-41.1, and C3-42.1. Furthermore, the metabiotic preparation of this strain can also alleviate Escherichia coli-induced endometritis in mice, improve uterine redness and swelling, and reduce pathological damage.

This invention provides a formulation capable of promoting normal decidualization of the endometrium. An endometrial decidualization promoter or improver containing an effective amount of pyrroloquinoline quinone or its salt is prepared. Furthermore, a cell fusion promoter or improver containing an effective amount of pyrroloquinoline quinone or its salt is also prepared.

The invention discloses a co-culture model based on endometrial stromal cell organoid and follicle organoid as well as a construction method and application of the co-culture model, and belongs to the technical field of reproductive medicine and cell engineering. The invention discloses a construction method of a co-culture model based on an endometrial stromal cell organ and a follicle organ. The construction method comprises a culture method of the follicle organ, a culture method of the endometrial stromal cell organ and co-culture of the follicle organ and the endometrial stromal cell organ. The 3D follicle-like organ and endometrial stromal cell-like organ co-culture system provided by the invention can reproduce in-vivo physiological conditions more truly, can be used for exploring a deciduous molecular mechanism, and also has the potential of constructing a high-throughput detection platform for drugs and environmental toxicants.

The invention provides a traditional Chinese medicine compound suppository for treating endometritis of livestock and a preparation method of the traditional Chinese medicine compound suppository, and relates to the technical field of veterinary drugs. The traditional Chinese medicine compound suppository consists of povidone iodine, a traditional Chinese medicine composition and a slow-release high polymer material matrix carrier, wherein the traditional Chinese medicine composition is prepared from thirteen traditional Chinese medicine components including philippine flemingia root, common andrographis herb, zanthoxylum dissitum, Chinese angelica, suberect spatholobus stem, motherwort herb, baical skullcap root, honeysuckle flower, weeping forsythia capsule, white paeony root, nutgrass galingale rhizome, heartleaf houttuynia herb and swordlike atractylodes rhizome. Besides the matrix carrier, the traditional Chinese medicine compound suppository only contains povidone iodine and traditional Chinese medicine composition components, the content of the povidone iodine component is low, and side effects are reduced. The traditional Chinese medicine compound suppository is non-toxic, small in irritation to vaginal mucosa of livestock, high in effective rate of treating endometritis of the livestock and good in anti-inflammatory effect. In the preparation process of the traditional Chinese medicine compound suppository, no organic solvent is used, pollution to the environment is avoided, and the preparation process is simple, convenient and easy to implement and low in cost.

The invention discloses application of circSLC26A4 in preparation of a medicine for promoting endometrial receptivity formation, and belongs to the technical field of biology. Research finds that overexpression circSLC26A4 can up-regulate mRNA and protein expression of an endometrial receptivity marker and improve the adhesion rate of an embryo on an endometrial, and shows that the circSLC26A4 has the application values of improving the endometrial receptivity, promoting embryo implantation and improving the reproductive capacity; in addition, overexpression of circSLC26A4 can also significantly improve the activity and proliferation number of endometrial epithelial cells, reduce the apoptosis rate and enhance the cell migration ability, and shows the application value of circSLC26A4 in the aspect of repairing endometrium. And a theoretical basis and a technical support are provided for developing related reagents or therapeutic drugs by taking circSLC26A4 as a target spot for regulating and controlling endometrial receptivity.

The application provides an evaluation method of an endometrial immune microenvironment, which evaluates the health condition of the endometrium through the aggregation state of immune cells. Compared with the prior art, the application has the following advantages: the application provides an evaluation method of an endometrial immune microenvironment and application thereof, and through the statistics of the proportion of immune cell aggregation foci on the endometrium, the microenvironment of the endometrium can be efficiently and accurately evaluated, and thus the early judgment of endometrium-related diseases such as endometrial chronic inflammation and pregnancy outcome can be effectively improved.

A method of determining at least one property of the endometrium, the method comprising: receiving image data associated with an endometrial biopsy specimen from a subject; inputting the data into a machine learning model; and using the machine learning model to determine the at least one property of the endometrium of the subject based on the image data.

The application discloses an endometrium-targeted mitochondrial function compensation system and application thereof. The endometrium-targeted mitochondrial function compensation system comprises a function unit, a delivery unit and a control unit. The function unit comprises a vesicle preparation formed by wrapping donor mitochondria with red blood cell membranes. The donor mitochondria are isolated mitochondria with complete inner and outer mitochondrial membranes and ridge structures or engineered mitochondria carrying exogenous mitochondrial DNA. The application first constructs a comprehensive system integrating biological active ingredients, targeted delivery and intelligent control, can directly and efficiently repair the mitochondrial function of endometrial cells, promote endometrial proliferation, and provides an innovative solution for treating related reproductive diseases. The system has complete design, clear effect and good clinical transformation prospect.

A device for collecting uterine fluid is described which can be used in vivo in both animals and humans, without said use causing dilution of fluid components or damage to the endometrium.

The invention provides an ultrasonic endometrial segmentation model construction method, a segmentation method and a device. The construction method comprises the following steps: acquiring uterus longitudinal section sample ultrasonic images of a patient at different time nodes and endometrial labeling areas corresponding to the sample ultrasonic images; image recognition and segmentation are carried out on the sample ultrasound image based on an initial model, a prediction category and an endometrium prediction area of each pixel are obtained, the initial model comprises a backbone network and a full convolutional network head, and the backbone network is a deep residual network; and performing parameter iteration on the initial model based on an overlapping rate between an endometrial labeling region and a prediction region and / or based on a difference between a prediction category and a labeling category of each pixel to obtain an ultrasonic endometrial segmentation model, and determining the labeling category based on the endometrial labeling region. According to the construction method, the segmentation method and the device provided by the invention, the stable segmentation performance can be kept even under the condition that the image quality is poor or the endometrium forms are varied.

The invention provides application of uterus-derived sphingomonas DS-28 and a metagen thereof in treatment of endometritis, and relates to the technical field of microorganisms. The sphingomonas DS-28 provided by the invention has uterus colonization ability and good ecological niche adaptability, and also has a remarkable curative effect in treatment of Escherichia coli induced endometritis. Animal experiments show that the strain preparation can effectively relieve red and swollen uterus, relieve pathological damage, remarkably reduce gene expression of TNF-alpha, IL-6 and IL-8 and inhibit an NF-kappa B signal channel, and the anti-inflammatory and tissue repair effects of the strain preparation are superior to those of other sphingomonas strains C3-25.1, C3-41.1 and C3-42.1. Meanwhile, the metastatic preparation of the strain can also relieve mouse escherichia coli endometritis, improve red and swollen uterus and relieve pathological injury.

The application discloses a kind of endometrial carcinoma molecular typing methods based on cascaded multiple instance learning, method is by the binary mask of tumor area generated to endometrial carcinoma whole section image preprocessing, to extract the image block in region of interest and construct instance set by dyeing normalization processing;Using PatchVMamba instance encoder, the feature of each image block in instance set is extracted to obtain the instance feature vector of each image block, and the instance feature bag of each whole section image is formed;Using attention mechanism, the instance feature bag is weighted and aggregated to obtain the slice-level feature representation;The slice-level feature is input into the three binary classifiers arranged in cascade to obtain the probability value corresponding to the three subtypes of endometrial carcinoma respectively, and a unified threshold is used for cascade judgment of the subtype classification of endometrial carcinoma to output the final typing result.The cascaded structure of the application makes the consistency of the typing result and the diagnosis of pathological experts high, and realizes accurate typing.

The present invention relates to an organ-on-a-chip device for mimicking the bone marrow microenvironment (1) comprising: a perivascular region (2) representing the perivascular niche; a central region (3) representing the central niche; an endosteal region (4) representing the endosteal niche; at least one fluid inlet channel (5); at least one channel for injecting cells, fluids and hydrogels (6) for each region (2, 3, 4); at least one containment port (7) to allow independent filling of the at least one channel for injecting cells, fluids and hydrogels (6); and at least one fluid outlet channel (8). The present invention also relates to a method for producing the device moulds using additive manufacturing, a method for producing the device from PDMS using soft lithography, a method for filling the device with hydrogel and performing three-dimensional culture, and use of the device.

Provided herein is a targeting molecule, wherein the targeting molecule binds to a surface molecule on an endometrial endothelial cell (EEC) in which expression of the surface molecule is upregulated.

This invention relates to the field of biotechnology, specifically, to a method for culturing endometrial organoids. By optimizing the endometrial organoid differentiation and culture system, compared to existing methods, the optimized system is simple to operate, does not require co-culturing with stromal cells to prepare organoids with more complete physiological functions, and can be cultured continuously for at least one year. The resulting organoids exhibit complete secretory function and a more pronounced ciliated cell phenotype.