63 results about "Endostelium" patented technology

The invention discloses an endometrium sampler, which relates to the technical field of medical instruments and is technically characterized by comprising a protective sleeve, a movable tube, a brush rod, a top bead, bristles and a handle, the protective sleeve and the movable pipe are both of a hollow tubular structure, and the movable pipe is arranged in the protective sleeve and can move in the axial direction of the protective sleeve. The brush rod is arranged in the movable pipe, one end of the brush rod is fixedly connected with the handle, and the other end of the brush rod is fixedly connected with the top bead. The section, close to the top bead, of the brush rod is a brush rod front end; the bristles are attached to the front end of the brush rod and incline towards the top bead, and the top bead is of a semi-surrounding cover-shaped structure. During sampling operation, the handle is rotated to drive the front end of the brush rod to rotate, so that the bristles cover an inverted pear-shaped uterine cavity area; after the bristles finish sampling, the movable pipe is controlled to move towards the top bead, so that the bristles are folded and protected by the movable pipe; the sampling efficiency, the sample integrity and the diagnosis accuracy can be improved.

The invention relates to the technical field of biology, and particularly provides an endometrial organ culture method. By optimizing the endometrial organoid differentiation culture system, compared with an existing culture mode, the optimized culture system is easy to operate, the organoid with more complete physiological functions can be prepared without co-culture with stromal cells, continuous culture can be conducted for at least one year or above, and the obtained organoid is complete in secretion function and free of secondary pollution. The phenotype of cilia cells is more obvious.

The present invention relates to a conjugate comprising a FAP targeting ligand and an effector for use in the treatment and / or diagnosis of endometriosis, as well as associated methods and kits.

The present invention provides a method of determining endometrial status using a sample (e.g., a plasma sample) from a subject, comprising: (a) performing an assay on a blood sample from a subject to determine a miRNA expression profile wherein the miRNA expression profile comprises a plurality of expression levels of miRNAs, and (b) analyzing the miRNA expression profile using a computer-based machine learning model to obtain a predicted score.

The application discloses a mesenchymal stem cell preparation and application thereof in thin endometrium repair, and comprises the following steps: adding Enterococcus durans CGMCC 1.3249 and Bacillus subtilis CGMCC 1.15792 supernatant as effective components into cultured mesenchymal stem cells; and the stem cell preparation obtained by the method can effectively restore the endometrial thickness of mice, increase the number of glands, and improve the pregnancy rate of the thin endometrium mice.

The present invention relates to the field of biomedical technologies and discloses a method for constructing a mouse model of ovarian endometriosis. The method includes: identifying the endometrial tissue of a syngeneic donor mouse and the bilateral ovaries of a recipient mouse; constructing an ectopic transplantation model of the recipient mouse and real-time monitoring the survival status of the recipient mouse at different postoperative stages; analyzing the growth of the ectopic endometrial tissue on the ovarian surface and identifying the tissue structure and pathological changes of the ectopic endometrial tissue; determining the lesion area of the ectopic transplantation model, analyzing the pathological characteristics and formation mechanism of the lesion area, and analyzing the degree of association between the ectopic transplantation model and human endometriosis; analyzing the simulation effect of the ectopic transplantation model on human endometriosis, and constructing a mouse model of ovarian endometriosis for the recipient mouse based on the modeling effect and the ectopic transplantation model. The present invention can improve the modeling rate of the endometriosis model and the accuracy of research results.

Provided herein is a targeting molecule, wherein the targeting molecule binds to a surface molecule on an endometrial endothelial cell (EEC) in which expression of the surface molecule is upregulated.

The invention discloses an application of miR-2355-3p in endometriosis. The research discovers that the RNA transcriptional level of miR-2355-3p in an ectopic endometrial tissue sample is obviously reduced for the first time; the mimics RNA overexpressed by miR-2355-3p is cloned to a lentiviral vector, lentiviral packaging is carried out, the miR-2355-3p mimic lentivirus is obtained, then the miR-2355-3p mimic lentivirus is used for transfecting ectopic endometrial interstitial cells, proliferation of the ectopic endometrial interstitial cells can be inhibited, apoptosis of the ectopic endometrial interstitial cells can be promoted, growth of ectopic endometrial tissues is inhibited, and the mimic RNA-mimic lentivirus can be used for treating the endometrial interstitial cells. And a new possibility is provided for developing the medicine for treating the endometriosis.

The present invention relates to drospirenone for use in a method for treating endometriosis, endometriosis associated pelvic pain (EAPP) and / or dysmenorrhea comprising administering drospirenone in a biphasic regimen. The present invention further relates to the use of drospirenone administered in such a biphasic regimen as a contraceptive and for inducing amenorrhea, as well as to pharmaceutical compositions and kits comprising drospirenone administered in such a biphasic regimen.

Methods for treating and non-invasively detecting endometriosis using menstrual discharge are provided.

The invention provides application of endometrial mesenchymal stem cell exosomes in atherosclerosis, and belongs to the technical field of biological medicine. The invention provides a bioactive preparation which is derived from specific tissues, has good safety and definite treatment directivity, can effectively intervene in the progress of atherosclerotic plaques and relieve pathological changes of blood vessels, and the treatment convenience and patient tolerance are remarkably improved through a non-invasive administration mode of the bioactive preparation. A brand new solution with excellent application potential is provided for clinical management of atherosclerosis.

The application discloses a preparation method of a 3D model of a maternal-fetal interface constructed by an endometrial organoid in vitro, and the method comprises the following steps: establishing a primary endometrial epithelial organoid and an endometrial stromal cell line in vitro; improving an extracellular matrix for assembling endometrial gland-like organoids and stromal cells; the constructed endometrial organoid model comprises gland cells and stromal cells; the endometrial organoid model is cultured by using a gas-liquid interface, so that the endometrial organoid model has a cavity epithelium and a gland structure; the endometrial organoid model simulates a menstrual cycle of the endometrium and performs function identification. The endometrial organoid model has the cavity epithelium and the gland epithelium for the first time, the tissue structure, the cell composition, the hormone-induced change, the gene expression characteristics of the organoid are similar to those in the body, and the organoid can reproduce the receptivity genes and the dynamicity of cilia which are necessary for embryo implantation in the body; the organoid can be used as a model for studying embryo implantation regulation, endometrial diseases and regeneration mechanisms.

Described herein are improved methods for the detection of endometriosis. Generally, the methods include, but are not limited to, applying machine learning algorithm to miRNA levels in order to detect, predict, diagnose, or monitor the presence or absence of endometriosis.

The invention belongs to the technical field of natural products and pharmaceutical industry, and provides a compound for improving endometrial aging. The compound is ganoderic acid C2 and is screened by the following method: inducing an endometrial organ to form an aging endometrial model by using D-galactose; contacting a compound to be detected with the senescence endometrial model, and detecting the proportion of beta-galactosidase and / or EdU positive cells; and screening a compound capable of remarkably reducing beta-galactosidase and / or remarkably improving the proportion of EdU positive cells, namely the compound for improving endometrial aging. The model constructed by the invention can fully simulate the physiological status and pathological change of the senescent endometrium, the screening reliability is high, the period is short, the ethical problem is avoided, and a reliable method is provided for subsequent screening of natural products.

The application belongs to the technical field of biotechnology and medicine, and specifically discloses a single-domain antibody-exosome compound (Nb-EMX2-Exos) targeting EMX2 as well as a preparation method and application thereof. The compound is prepared by covalently coupling a single-domain antibody (Nb-EMX2) specifically binding to EMX2 protein to the surface of endometrial stem cell-derived exosomes (ESC-Exos). In-vitro experiments show that the compound can specifically recognize and efficiently internalize into damaged endometrial stromal cells with high expression of EMX2, and significantly promote the migration and proliferation of the cells. In-vivo experiments further prove that the compound can precisely target and enrich in damaged endometrial tissue, effectively promote the structural repair (thicken the endometrium and reduce fibrosis) of the endometrium, and completely restore the fertility function. The application provides a novel targeted treatment preparation for solving the problems of poor targeting of natural exosomes and limited treatment effect, and has important application value in the treatment of endometrial injury repair and related infertility.

The present invention relates to a composition for endometrial regeneration containing a uterus-derived decellularized extracellular matrix (UdECM) and a manufacturing method therefor. Characterized by containing uterus-derived decellularized extracellular matrix (UdECM), the composition can exhibit the effect of enhancing the regeneration, receptivity, and implantation capabilities of the endometrium.

The invention discloses a novel application of a compound beta-alanine (beta-alanine) in preparation of a medicine for improving oocyte quality and treating ovarian endometriosis (OEM) related infertility, and belongs to the technical field of biological medicines. An OEM animal model proves that beta-alanine can remarkably improve reproductive function disorder of model animals, specifically, follicular dysplasia caused by endometriosis is effectively relieved, the fertility is improved, and the litter size is increased; in the aspect of oocyte quality, the compound can promote normal discharge of a first polar body in an oocyte in-vitro maturation process, reduce cytoplasm fragmentation, recover spindle body form and microtubule arrangement, enhance mitochondrial membrane potential stability and maintain the integrity of an ultrastructure. The results show that beta-alanine can reverse oocyte quality reduction caused by ovarian endometriosis in multiple dimensions, has a definite treatment potential, and provides an important candidate molecule for developing a novel assisted reproduction intervention strategy for targeting oocyte health.

This invention provides the application of a uterine-derived Sphingomonas DS-28 strain and its metabolites in the treatment of endometritis, relating to the field of microbial technology. The Sphingomonas DS-28 strain provided by this invention possesses uterine colonization ability, good niche adaptability, and significant efficacy in treating Escherichia coli-induced endometritis. Animal experiments show that the preparation of this strain can effectively alleviate uterine redness and swelling, reduce pathological damage, and significantly reduce [the condition / progression]. TNF-alpha , IL-6 , IL-8 It inhibits gene expression and suppresses the NF-κB signaling pathway, exhibiting superior anti-inflammatory and tissue repair effects compared to other Sphingomonas strains C3-25.1, C3-41.1, and C3-42.1. Furthermore, the metabiotic preparation of this strain can also alleviate Escherichia coli-induced endometritis in mice, improve uterine redness and swelling, and reduce pathological damage.

This invention provides a formulation capable of promoting normal decidualization of the endometrium. An endometrial decidualization promoter or improver containing an effective amount of pyrroloquinoline quinone or its salt is prepared. Furthermore, a cell fusion promoter or improver containing an effective amount of pyrroloquinoline quinone or its salt is also prepared.

The invention discloses a co-culture model based on endometrial stromal cell organoid and follicle organoid as well as a construction method and application of the co-culture model, and belongs to the technical field of reproductive medicine and cell engineering. The invention discloses a construction method of a co-culture model based on an endometrial stromal cell organ and a follicle organ. The construction method comprises a culture method of the follicle organ, a culture method of the endometrial stromal cell organ and co-culture of the follicle organ and the endometrial stromal cell organ. The 3D follicle-like organ and endometrial stromal cell-like organ co-culture system provided by the invention can reproduce in-vivo physiological conditions more truly, can be used for exploring a deciduous molecular mechanism, and also has the potential of constructing a high-throughput detection platform for drugs and environmental toxicants.

The invention provides a traditional Chinese medicine compound suppository for treating endometritis of livestock and a preparation method of the traditional Chinese medicine compound suppository, and relates to the technical field of veterinary drugs. The traditional Chinese medicine compound suppository consists of povidone iodine, a traditional Chinese medicine composition and a slow-release high polymer material matrix carrier, wherein the traditional Chinese medicine composition is prepared from thirteen traditional Chinese medicine components including philippine flemingia root, common andrographis herb, zanthoxylum dissitum, Chinese angelica, suberect spatholobus stem, motherwort herb, baical skullcap root, honeysuckle flower, weeping forsythia capsule, white paeony root, nutgrass galingale rhizome, heartleaf houttuynia herb and swordlike atractylodes rhizome. Besides the matrix carrier, the traditional Chinese medicine compound suppository only contains povidone iodine and traditional Chinese medicine composition components, the content of the povidone iodine component is low, and side effects are reduced. The traditional Chinese medicine compound suppository is non-toxic, small in irritation to vaginal mucosa of livestock, high in effective rate of treating endometritis of the livestock and good in anti-inflammatory effect. In the preparation process of the traditional Chinese medicine compound suppository, no organic solvent is used, pollution to the environment is avoided, and the preparation process is simple, convenient and easy to implement and low in cost.

The invention discloses application of circSLC26A4 in preparation of a medicine for promoting endometrial receptivity formation, and belongs to the technical field of biology. Research finds that overexpression circSLC26A4 can up-regulate mRNA and protein expression of an endometrial receptivity marker and improve the adhesion rate of an embryo on an endometrial, and shows that the circSLC26A4 has the application values of improving the endometrial receptivity, promoting embryo implantation and improving the reproductive capacity; in addition, overexpression of circSLC26A4 can also significantly improve the activity and proliferation number of endometrial epithelial cells, reduce the apoptosis rate and enhance the cell migration ability, and shows the application value of circSLC26A4 in the aspect of repairing endometrium. And a theoretical basis and a technical support are provided for developing related reagents or therapeutic drugs by taking circSLC26A4 as a target spot for regulating and controlling endometrial receptivity.

The application provides an evaluation method of an endometrial immune microenvironment, which evaluates the health condition of the endometrium through the aggregation state of immune cells. Compared with the prior art, the application has the following advantages: the application provides an evaluation method of an endometrial immune microenvironment and application thereof, and through the statistics of the proportion of immune cell aggregation foci on the endometrium, the microenvironment of the endometrium can be efficiently and accurately evaluated, and thus the early judgment of endometrium-related diseases such as endometrial chronic inflammation and pregnancy outcome can be effectively improved.

Methods and devices for non-thermal treatment of endometriosis. The non-thermal treatment of endometrial lesions results in electroporation of the endometrial cells, and ultimately necrosis of the lesion. Lesions are identified and electric field parameters determined to substantially remove all of the endometrial cells in the target lesion. Application of the electric field to the lesion creates a treatment zone where the cells necrose, leaving the cellular matrix mostly undisturbed. Devices include a generator capable of applying an electric field to an endometrial lesion through one or more electrodes.

The present invention discloses an algorithm for automatically identifying and quantifying endometrial peristalsis in magnetic resonance images, comprising the following steps: step one, constructing a 3D-UNET network model; step two, identifying endometrial exfoliation movement; step three, identifying peristaltic wave conduction in the uterine juncture; step four, quantifying the amplitude of endometrial peristalsis; and step five, determining the direction of endometrial peristalsis. The present invention realizes automatic identification of endometrial exfoliation movement and peristaltic wave conduction in the uterine juncture based on the 3D-UNET network model, can automatically quantify the amplitude of endometrial peristalsis, and determine the direction of endometrial peristalsis. Compared with the existing method relying on naked eye observation by radiologists, the identification result of the present invention has better objectivity and repeatability, can obtain the standard range of female uterine movement-related parameters by expanding the amount of data, provide a reference standard for clinical application, and expand the application of endometrial peristalsis as a uterine function evaluation standard in clinical practice.

The application relates to the technical field of traditional Chinese medicines, in particular to application of a traditional Chinese medicine composition in preparation of medicines for preventing and treating endometriosis, wherein the traditional Chinese medicine composition comprises Gleditsia sinensis L. thorns, giant knotweed, dandelion, Sargentgloryvine stem, radix codonopsis, smilax, red peony root, salvia miltiorrhiza, lindera and corydalis. Experimental results show that the number of writhing of the mice given the traditional Chinese medicine composition is significantly reduced, the volume of ectopic endometrium is significantly reduced, and the secretion of serum PGE2 and PGF2 alpha is obviously reduced, indicating that the traditional Chinese medicine composition has a good treatment effect on endometriosis.

A method of determining at least one property of the endometrium, the method comprising: receiving image data associated with an endometrial biopsy specimen from a subject; inputting the data into a machine learning model; and using the machine learning model to determine the at least one property of the endometrium of the subject based on the image data.

The invention belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to a pharmaceutical composition for treating endometritis and a preparation method and application thereof. The pharmaceutical composition for treating endometritis, provided by the invention, is prepared from the following raw materials in parts by weight: 10 to 30 parts of radix salviae miltiorrhizae, 8 to 25 parts of rhizoma curcumae, 8 to 25 parts of rhizoma sparganii, 5 to 15 parts of radix gentianae, 10 to 30 parts of herba taraxaci, 10 to 25 parts of fructus forsythiae, 8 to 20 parts of rhizoma chuanxiong, 5 to 15 parts of fructus cnidii, 8 to 20 parts of rhizoma cyperi, 15 to 35 parts of radix astragali seu hedysari and 3 to 10 parts of liquorice root. The medicine composition has the main effects of promoting blood circulation to remove blood stasis and clearing away heat and toxic materials, has the auxiliary effects of regulating qi, eliminating dampness, warming yang and the like, and achieves the purpose of treating endometritis by improving blood circulation of endometrium, eliminating inflammation and regulating endocrine through the synergistic effect of multiple aspects.