19 results about "Endometrial tissue" patented technology

The invention discloses a Raman spectrum-based endometrial tissue spectral data processing method and equipment, and relates to the technical field of data processing. The method comprises the following steps: firstly, calculating a gradient change sequence of to-be-processed spectral data, carrying out continuity verification in combination with wavelength and width, and accurately dividing a Raman characteristic peak interval and a background baseline interval; then, iterative baseline fitting processing based on a Raman peak protection mechanism is adopted, blood fluorescence interference is eliminated, and pure Raman spectrum data is obtained. And further, inputting the pure spectral data into a pre-constructed segmented fusion feature extraction network, extracting local wave band features, fusing the local wave band features with global features, and outputting a data classification result. According to the embodiment of the invention, the problem of difficult feature extraction caused by low signal-to-noise ratio of a bleeding sample and non-uniform background in related technologies can be effectively solved, the integrity of spectrum fingerprint information is ensured while strong fluorescence interference is thoroughly eliminated, and the accuracy of data feature extraction and the robustness of classification and recognition are improved.

The invention relates to the technical field of medical care information processing, and discloses a traditional Chinese medicine prescription efficacy evaluation system for treating intrauterine adhesion, comprising: a data preprocessing module for acquiring endometrial dynamic response gray time sequence characteristic data before and after a subject takes a prescription; the phase analysis module is used for determining a phase deviation distribution coefficient according to the phase angle distribution of the gray scale data of the reference pixel and the response pixel; the frequency domain inversion module is used for constructing an energy transfer gain spectrum by calculating a power ratio and identifying a characteristic resonant frequency; the evaluation index output module is used for generating an evaluation index for evaluating the viscoelasticity improvement degree of the endometrial tissue according to the deviation value of the characteristic resonant frequency in combination with the variation of the phase deviation distribution coefficient. And the quantitative accuracy of evaluation is improved.

Disclosed herein are methods for obtaining endometrial stromal fibroblast cells from pluripotent stem cells, such as induced pluripotent stem cells. The present disclosure also provides methods of obtaining a three-dimensional, multilayered endometrial tissue composition. Methods of using the cells and tissue compositions in drug screening and therapeutic applications are also provided.

An electrotherapeutic device can be configured for at least partial insertion into a patient. The device can include a shaft including a proximal portion and a distal portion, a gas-delivery lumen extending between the proximal portion and the distal portion of the shaft, and one or more electrodes located near the distal portion of the shaft. The electrodes can include a plasma activation electrode for generating plasma from gas delivered to the distal portion of the shaft via the gas-delivery lumen, and an ablation electrode for generating electromagnetic ablation energy. The medical device can be used in a method of treating endometriosis including delivering a gas toward an in vivo treatment site within a patient, generating plasma from the gas on or near the in vivo treatment site using a plasma activation electrode, exposing the treatment site to the plasma, and applying electromagnetic ablation energy to treat endometrial tissue.

The application belongs to the technical field of biotechnology and medicine, and specifically discloses a single-domain antibody-exosome compound (Nb-EMX2-Exos) targeting EMX2 as well as a preparation method and application thereof. The compound is prepared by covalently coupling a single-domain antibody (Nb-EMX2) specifically binding to EMX2 protein to the surface of endometrial stem cell-derived exosomes (ESC-Exos). In-vitro experiments show that the compound can specifically recognize and efficiently internalize into damaged endometrial stromal cells with high expression of EMX2, and significantly promote the migration and proliferation of the cells. In-vivo experiments further prove that the compound can precisely target and enrich in damaged endometrial tissue, effectively promote the structural repair (thicken the endometrium and reduce fibrosis) of the endometrium, and completely restore the fertility function. The application provides a novel targeted treatment preparation for solving the problems of poor targeting of natural exosomes and limited treatment effect, and has important application value in the treatment of endometrial injury repair and related infertility.

The invention discloses a model building method based on animal endometrial epithelium organoid and application of the model building method, and relates to the technical field of cell engineering and organoid. According to the method, healthy cow endometrial tissue serves as a raw material, a model is built through the steps of pretreatment, enzymolysis, cell cleaning and screening, seed gum culture, passage, cryopreservation resuscitation and the like, and the model is built through Hamp; e, verifying morphological integrity through dyeing, and detecting E-cadherin expression through IHC dyeing to confirm cell specificity. The animal organ model restores the in-vivo three-dimensional microenvironment and species specificity, overcomes the limitation of traditional two-dimensional culture and mouse models, can accurately simulate the pathological process of endometritis, is applied to research on the pathogenesis of endometritis of dairy cows, can also efficiently screen anti-inflammatory drugs and evaluate the drug effect, and has a wide application prospect. A reliable in-vitro tool is provided for disease prevention and treatment, and healthy development of the dairy cow breeding industry is assisted.

This invention belongs to the field of biotechnology, and particularly relates to the use of the SPON2 gene or its protein as a target in the diagnosis or treatment of endometriosis. Supported by multi-omics and single-cell level data and tested with clinical samples, this invention, for the first time, elucidates the correlation between SPON2 and endometriosis (EM), revealing that high expression of SPON2 in eutopic and ectopic endometrial fibroblasts in endometriosis patients enhances their proliferation, invasion, and migration abilities. Inhibiting SPON2 gene expression or inhibiting SPON2 protein function reduces the proliferation and invasion abilities of endometrial tissue, as well as lesion migration, invasion, and fibrosis, thus improving the condition of endometriosis. This suggests that the SPON2 gene or its protein can serve as a diagnostic or therapeutic target, providing new ideas and directions for the clinical diagnosis and targeted therapy of endometriosis.

The invention belongs to the technical field of application of traditional Chinese medicines, and particularly relates to application of a Dan'e Fukang preparation in preparation of a medicine for treating dysfunctional uterine bleeding. Pharmacological experiments prove that Dan'e'fukang can shorten the blood coagulation time and bleeding time of mice, and increase the uterine coefficient, estradiol level and progesterone level of rats without ovulation type dysfunctional uterine bleeding and the VEGF protein expression quantity of endometrial tissues of the rats. The Dan'e Fukang provides more medication choices for clinical treatment of dysfunctional uterine bleeding diseases, and has the advantages of good effect, many years of clinical use experience and high safety.

Provided herein include synthetic endometrial tissues and uterus-like structures for assessing embryo implantation and growth in vitro. Provided herein also include methods and devices for making the synthetic endometrial tissues and uterus-like structures and uses thereof to culture embryos (e.g., human embryos). The methods and synthetic endometrial tissues and structures generated herein can be used to culture pre-implantation embryos (e.g., human embryos) to reach a post-implantation stage.

This invention relates to the pharmaceutical field, specifically to a drug for the prevention or treatment of endometrial fibrosis, comprising poloxamer and spermidine; poloxamer is added to the spermidine solution and swelled at 3-5°C until completely dissolved to form a liquid precursor; the liquid precursor undergoes a phase transition at 35-40°C to form a gel; the liquid precursor is injected into the uterine cavity to reduce the expression of αSMA in the endometrial tissue, inhibit the transformation of stromal cells into myofibroblasts, and promote angiogenesis.

A hysteroscopic specimen collection device includes a connecting piece and a collection bag. The connecting piece has an operating through hole and a first adhesive layer on its back. The lower end of the connecting piece is connected to the opening side of the collection bag. A first filter and a second filter are installed sequentially from top to bottom inside the collection bag. The first filter is detachably installed inside the collection bag. The diameter of some filter holes in the second filter is smaller than the diameter of some filter holes in the first filter. A fixing plate is provided on the back of the connecting piece or the collection bag to assist in fixing the collection device during use. The first and second filters of this invention facilitate the separation of tissue specimens and distension fluid flowing into the collection bag, enabling specimen collection and separation of specimens of different sizes, such as easily separating uterine fibroid tissue and endometrial tissue. The first adhesive layer and the fixing plate together fix the collection device. The overall structure is simple, convenient, and practical.

The application discloses a polypeptide AIUAP for preventing or assisting in preventing formation of intrauterine adhesion and application thereof. The polypeptide AIUAP for preventing, assisting in preventing or treating formation of intrauterine adhesion has an amino acid sequence of TFGGAPGFPLGSPLSSPVFPR (SEQ ID NO. 1), and is composed of 21 amino acids. The application discloses application of the polypeptide AIUAP in preparation of a medicine for preventing, assisting in preventing or treating intrauterine adhesion. In the research, a method of tandem mass spectrometry is used to screen endogenous polypeptides differentially expressed in intrauterine adhesion tissues and normal proliferative endometrial tissues, and it is found that the polypeptide AIUAP highly expressed in the intrauterine adhesion tissues can inhibit formation of fibrosis. Since the AIUAP has the characteristics of small molecular weight, self-source and small toxicity, the AIUAP can become an important auxiliary medicine for preventing and treating intrauterine adhesion.

The invention relates to the technical field of medical equipment, in particular to a sampling tool for endometrium biopsy and a sampling method.The sampling tool comprises a suction tube, a scraper composed of a plurality of scraping wires is arranged at the far end of the suction tube, and one end of the scraper is inserted into an opening in the far end of the suction tube and connected with the suction tube; a channel for endometrial tissues to enter and exit is arranged between the scraper and the inner wall of the suction tube; the near end of the suction pipe is provided with a pressure adjusting part used for adjusting the pressure intensity in the suction pipe. The scraping wires extend in the axial direction of the suction pipe, the two ends, in the same direction, of the multiple scraping wires are fixedly connected together, and the middles of the multiple scraping wires are spliced to form a hollow ellipsoid structure. The problem that in the suction process of a suction tube in the prior art, in the suction process, a suction inlet is directly covered and blocked by endometrial tissue which is not separated, and contusion is caused to local tissue in a blocked area is solved.

This utility model discloses a disposable endometrial sampler, including an outer tube, a negative pressure suction head connected to one end of the outer tube by a thread, and a sampling bottle connected to one end of the outer tube by a thread. The negative pressure suction head and the sampling bottle are sequentially installed inside the outer tube. A core rod is inserted inside the outer tube, and a silicone ring is provided at the end of the core rod inside the outer tube. The core rod pulls the silicone ring to move inside the outer tube, so that endometrial tissue is sucked into the outer tube through the negative pressure suction head. This utility model uses the sampling bottle to directly obtain endometrial tissue inside the outer tube, reducing the loss and contamination of endometrial tissue collected inside the outer tube during the testing and transfer process.

The invention discloses a noninvasive biomarker related to an endometrial periimplant window phase and application of the noninvasive biomarker. The biomarker comprises 150 miRNAs (micro Ribonucleic Acid), namely, hsa-miR-1290, hsa-miR-511-3p, hsa-miR-1246, hsa-miR-371b-5p, hsa-miR-887-3p, hsa-miR-874-3p, hsa-miR-181a-3p, hsa-miR-873-3p and the like, and is characterized in that the miRNAs can be used for preparing the biomarker. The marker disclosed by the invention can be used for non-invasively and accurately judging whether endometrial tissues have receptivity or not, and has important significance on whether an embryo is successfully transplanted or not.

Provided herein include synthetic endometrial tissues and uterus-like structures for assessing embryo implantation and growth in vitro. Provided herein also include methods and devices for making the synthetic endometrial tissues and uterus-like structures and uses thereof to culture embryos (e.g., human embryos). The methods and synthetic endometrial tissues and structures generated herein can be used to culture pre-implantation embryos (e.g., human embryos) to reach a post-implantation stage.

The invention provides application of hesperetin in preparation of a medicine for treating intrauterine adhesion, and belongs to the technical field of endometrial repair. It is found that hesperetin can promote endometrial regeneration, inhibit endometrial fibrosis and promote endometrial tissue angiogenesis and can be used for preparing the medicine for preventing and / or treating intrauterine adhesion. And hesperetin is a natural flavone compound, exists in various citrus fruits, has excellent safety and a wide source, and has a good application prospect.

The application provides application of hydroxysafflor yellow A in preparation of medicines for preventing, treating and / or relieving endometriosis, and belongs to the technical field of biological medicines. The application provides application of hydroxysafflor yellow A in preparation of medicines for preventing, treating and / or relieving endometriosis. Hydroxysafflor yellow A can inhibit migration and / or diffusion of endometriosis cyst, inhibit migration, invasion and abnormal planting of endometrial tissue and / or endometriosis cells outside the uterine cavity, relieve inflammatory reaction caused by endometriosis, and effectively curb lesion invasion and disease progression of endometriosis. As a main active component of safflower, hydroxysafflor yellow A provides a new direction for manufacturing new medicines for treating endometriosis by using safflower.

The application belongs to the field of life science, and relates to a non-invasive endometrial receptivity detection method. The detection method detects the methylation state of free DNA in a donor peripheral blood sample to determine whether the endometrial receptivity window of the donor is deviated. The detection method provided by the application takes free DNA in peripheral blood as a detection object, determines the endometrial receptivity of the donor by detecting the methylation state of a specific region of the free DNA, is used for assisting the in-vitro fertilization process, does not need to biopsy endometrial tissue, reduces the physical and mental burden of the patient, and realizes non-invasive detection. The detection route is simple, the operation is simple, the cost is lower, the clinical detection efficiency can be significantly improved, and the application range is wider.