The invention belongs to the technical field of animal experiment model construction, and relates to a construction method of a porcine endometrial
injury model. The construction method is non-traumatic, can simulate the
pathological state of clinical
intrauterine adhesion on the premise of not damaging the full-thickness structure of the
uterus of a pig, and comprises the following specific steps: intramuscularly injecting 1.5 mg / kg of Shutai to calm an experimental pig, then carrying out
anesthesia induction and analgesia, intramuscularly injecting
phloroglucinol and probing the
cervix uteri after the
anesthesia induction succeeds and
vital signs are stable, and carrying out
intramuscular injection on the experimental pig. The
cervix uteri is ensured to be relaxed; a sixth
curette enters the
uterus body from the
cervix gently and slowly, and the
endometrium is mechanically scraped until the surface is coarse; and then injecting 95%
ethanol and staying for a
specific time, and finally removing the residual
ethanol. According to the method,
laparotomy is not needed, the operation path is simple, the full-layer structure of the uterine
muscular layer and the serosal layer can be completely reserved, the modeling effect is highly similar to the clinical
intrauterine adhesion condition, the success rate is as high as 95%, the
repeatability is good, the modeling effect is stable, and a standardized
large animal model capable of being popularized is provided for
intrauterine adhesion mechanism research and clinical intervention evaluation.