17results about How to "Reduced purification steps" patented technology

The invention discloses a method for preparing polyetherketoneketone by using a microchannel reactor, and belongs to the technical field of polymer synthesis, a certain amount of sulfur dioxide and Lewis base are added in the reaction to improve the catalytic activity of Lewis acid, improve the selectivity, increase the solubility of a polymer and avoid precipitation of a product in a microchannel; the reaction selectivity is improved through the micro-channel reactor, generation of side reaction impurities of tonnage alcohols is avoided, the obtained product is high in purity, and the step of purifying the intermediate 1, 4-bis (4-phenoxybenzoyl) benzene is omitted; the molecular weight distribution of the product is narrower; the materials are all fed in a liquid mode after being dissolved, and introduction of Lewis acid and mechanical impurities in other raw materials and reagents is avoided through filter pressing; and continuous production can be realized, the production efficiency is improved, and the production cost is reduced.

The invention relates to the field of metal complexes, in particular to a complex as well as a preparation method and application thereof. The complex has a structure as shown in a formula (a), and in the formula (a), M is one of IVB group metals; r1 is selected from at least one of aryl, aryl containing a first substituent, heterocyclic aryl and heterocyclic aryl containing a second substituent, and R2 is selected from at least one of alkyl, halogenated alkyl, cyano, ester group, alkenyl and alkynyl. The ligand part of the metal complex as shown in the formula (a) has an asymmetric structure and has better catalytic activity on polymerization reaction; # imgabs0 #

The invention belongs to the technical field of pentamethylene diamine separation, and provides a method for synthesizing nylon salt from 1, 5-pentamethylene diamine. The method comprises the following steps: mixing a free 1, 5-pentamethylene diamine solution and n-butyl alcohol, and sequentially standing and separating liquid to obtain an extraction phase; performing reduced-pressure rotary evaporation on the extraction phase to obtain a mixed solution; the mixed solution comprises n-butyl alcohol, water and 1, 5-pentanediamine; and mixing the mixed solution with the adipic acid solution, reacting the mixture, and cooling and crystallizing to obtain the nylon salt. The extraction agent can be simultaneously used as a reaction solvent and can directly react in an extraction phase, so that the purification step is omitted, the repeated operation of purifying the pentamethylene diamine from the solvent and adding the pentamethylene diamine back to the solvent is avoided, and the salt forming process is simplified.

The present invention discloses a method for constructing a trace nucleic acid library. The method comprises the following steps: (1) obtaining a fragmented single-stranded DNA or cDNA that has been phosphorylated at the 5' end and hydroxylated at the 3' end; (2) performing 3' end auxiliary connection of the DNA fragments obtained in step (1) to extend into a double-stranded DNA; (3) connecting the 5' end of the double-stranded DNA obtained in step (2) to a linker, and then performing dual-template substrate amplification to obtain a library. The present invention improves the efficiency of preparing a trace library and reduces the purification steps, which is of great significance for constructing a library with a trace amount of DNA starting material.

This invention relates to the field of catalytic dehydrogenation technology for hydrogen storage materials, and discloses a method for dehydrogenating hydrogen storage materials. The method includes: contacting the hydrogen storage material sequentially with a first dehydrogenation catalyst and a second dehydrogenation catalyst under dehydrogenation reaction conditions; wherein the hydrogen storage material includes a hydrogenated N-containing heterocyclic hydrogen storage material and an impurity component; the first dehydrogenation catalyst includes a first support and a first active component supported on the first support, the first active component being Cu; the second dehydrogenation catalyst includes a second support and a second active component supported on the second support, the second active component being at least one of noble metals; the volume ratio of the first dehydrogenation catalyst to the second dehydrogenation catalyst is 1:(1-9). The dehydrogenation method provided by this invention can adapt to hydrogen storage materials containing impurity components, exhibiting high reactivity and good long-term operational stability.

The invention provides a chitosan-dimethyl itaconate conjugate and a synthetic method thereof. The synthetic method comprises the following steps: dissolving CS in deionized water; dissolving DMI in absolute ethyl alcohol; then gradually dropwise adding the dimethyl itaconate solution into the chitosan solution, adding a proper amount of absolute ethyl alcohol, and fully dissolving reactants to form a uniform solution; adding sodium hydroxide to adjust pH; primary amine groups on a CS chain are used as nucleophilic reagents to attack ester carbonyl carbon atoms of DMI, nucleophilic substitution reaction is carried out, and finally amido bonds are formed. Heating reactants under a dark condition, slowly stirring to complete reaction, and ventilating and drying in a drying oven to form a thin film; and eluting the thin film with absolute ethyl alcohol to obtain a final product CS-DMI. The chitosan-dimethyl itaconate synthesized by the method disclosed by the invention has good biocompatibility and anti-inflammatory and antibacterial properties. A strategy and reference are provided for coupling the small molecule medicine to the chitosan, developing a water-soluble high polymer material and applying the small molecule medicine to a medical material.

The present invention relates to the field of amino acid preparation, and in particular to a method for preparing highly stable L-aspartic acid by synergistic catalysis, which realizes efficient synergy by combining histidine-modified UiO-67 enzyme complex and amino acid-modified ZIF-8 / Pebax-1657 mixed matrix membrane technology. The coordination of histidine and UiO-67 enhances the stability and activity of the enzyme, and improves the yield and purity of L-aspartic acid. At the same time, the high selectivity and permeability of the modified ZIF-8 / Pebax-1657 membrane improve the separation efficiency, effectively purify L-aspartic acid, improve the preparation efficiency and product quality of L-aspartic acid, reduce environmental pollution, and realize green production and cost control.

The invention discloses a method for recovering phosphorus and fluorine from sludge containing phosphorus and fluorine. The method is used for resourceful treatment of the sludge containing phosphorus and fluorine in the phosphorus chemical industry. The method comprises the steps that sludge containing phosphorus and fluorine and experimental water are mixed in proportion to obtain sludge slurry, the sludge slurry is fully stirred so that the sludge slurry can be evenly dispersed, and the experimental water is consistent with field water of phosphorus chemical enterprises and is slag field return water or preparation process water; adjusting the solid content of the sludge slurry to 20%-30%; adding sulfuric acid as a reaction reagent into the sludge slurry, and carrying out a stirring reaction to obtain a mixture; carrying out solid-liquid separation on the mixture through a filtering system, and collecting filtrate containing soluble phosphorus and fluorine and solid-phase residues obtained through separation; and recovering the filtrate for a phosphorus chemical production process to realize phosphorus and fluorine resource recovery, and carrying out subsequent treatment on the solid-phase residue to realize sludge residue reduction.

The invention relates to a method for preparing dapagliflozin and an intermediate thereof, which comprises the following steps: reacting a compound as shown in a formula II with a compound as shown in a formula III in an organic solvent under the action of organic metal lithium, dropwise adding reaction liquid into water after the reaction is finished, and quenching to obtain a compound as shown in a formula IV, and reacting the compound as shown in the formula IV with methanol under an acidic condition to obtain a compound as shown in a formula V. The method provided by the invention has the advantages of mild conditions, few byproducts, short time consumption and the like. # imgabs0 #

The invention discloses a method for measuring free amine in amino-terminated liquid nitrile rubber (ATBN) by a gas chromatography technology, which comprises the following steps: weighing an amino-terminated liquid nitrile rubber (ATBN) sample in a centrifuge tube, adding ethanol to precipitate macromolecules to enrich free amine, enabling the free amine to be completely extracted and enriched, and centrifuging to obtain a supernatant; and combining the supernatant enrichment liquid for gas chromatography detection. According to the present invention, the gas chromatography external standard method is adopted to achieve the rapid detection of the free amine in the amino-terminated liquid nitrile rubber (ATBN), and the method has advantages of high repeatability, high test data sensitivity, high accuracy, low instrument operation cost and suitableness for industrial production, and can achieve the purpose of monitoring the reaction raw materials during the ATBN industrial synthesis process.

The invention discloses a bioengineering-based elastin-like protein purification method, which comprises: 1) obtaining an engineering bacterium thallus expressing elastin-like protein, and re-suspending the thallus by using a buffer solution to obtain a thallus re-suspension; 2) adding lysozyme and DNA enzyme into the thallus resuspension, heating to 35-55 DEG C, and stirring for 0.5-2 hours to obtain a thallus lysate; 3) rapidly cooling to 2-10 DEG C, and centrifuging to obtain clear supernate; 4) purifying the supernate through cation exchange chromatography to obtain a protein solution; 5) performing ultrafiltration desalting treatment on the protein liquid to obtain a pure protein aqueous solution; and 6) freeze-drying to obtain the protein. According to the method, the pure protein can be obtained by purifying the protein twice in a thermal cracking mode, the protein recovery efficiency is improved, the production cost is low, meanwhile, efficient purification of the K protein independent of His tag peptide is achieved, and the method is suitable for industrial production.

The present application relates to the technical field of biological chemical industry detection, and particularly relates to a method for determining the content of 7-dehydrocholesterol in yeast, which realizes efficient extraction and accurate determination of 7-DHC in yeast cells through the steps of yeast cell wall breaking treatment, ultrasonic-assisted extraction, high performance liquid chromatography-ultraviolet detection (HPLC-UV) quantitative analysis. The recovery rate of the method can reach 98.8%-99.4%, and the relative standard deviation (RSD) is less than or equal to 2.1%. The method has the advantages of simple operation, high sensitivity and good repeatability, and can be widely applied to the screening of 7-DHC yeast engineering bacteria, the monitoring of the fermentation process and the quality control of industrialized products.

The invention relates to the technical field of thallus fermentation, and discloses a novel thallus fermentation process and equipment, and the novel thallus fermentation process comprises the following steps: S1, forming an aqueous two-phase system in a fermentation medium, the aqueous two-phase system comprises polyethylene glycol and phosphate, the concentration of PEG is 15% w / w, and the concentration of phosphate is 10% w / w; s2, inoculating a microbial strain into the culture medium, and controlling fermentation conditions including the temperature of 30 DEG C, the pH value of 6.57.0 and the dissolved oxygen content of 4060%; s3, separating the PEG phase from the phosphate phase through a semi-permeable membrane system in the fermentation process, and removing a target product in the PEG phase in real time through a pump circulation system; and S4, collecting the product, separating out the target product from the PEG phase by cooling or diluting, and recovering the PEG and the phosphate phase for recycling. By improving fermentation efficiency and product purity and reducing production cost and resource waste, the problems of low fermentation efficiency, low product purity and high cost in the prior art are solved.

The invention relates to a method for preparing palmitoyl tetrapeptide-7 through liquid-phase polypeptide synthesis, which comprises the following steps: step 1, obtaining palmitoyl chloride from palmitic acid through acylating chlorination, and then generating Pal-Gly-OH with glycine through substitution reaction; the preparation method comprises the following steps: 1 ', synthesizing N-t-butyloxycarboryl-glutamyl-proline benzyl ester from N-t-butyloxycarboryl-glutamine and proline benzyl ester hydrochloride through dipeptide condensation, and obtaining glutamyl-proline benzyl ester hydrochloride (H-Gln-Pro-OBzl.HCl) through deprotection; step 2, carrying out tripeptide condensation, so as to obtain palmitoyl-glycyl-glutamyl-proline benzyl ester (Pal-Gly-Gln-Pro-OBzl) from Pal-Gly-OH and H-Gln-Pro-OBzl. HCl (hydrogen chloride); step 3, removing a protecting group by virtue of a hydrolysis reaction, so as to prepare palmitoyl-glycyl-glutamyl-proline (Pal-Gly-Gln-Pro-OH); and a step 4 of preparing an active ester Pal-Gly-Gln-Pro-OSu from the Pal-Gly-Gln-Pro-OH by means of ester condensation, and then obtaining the palmitoyl tetrapeptide-7 from the Pal-Gly-Gln-Pro-OSu and arginine by means of tetrapeptide condensation.

This invention discloses a large-scale preparation method for high-purity phycobiliproteins, belonging to the field of purification technology for active ingredients in natural products. This invention utilizes the addition of electronegative polysaccharides or oligosaccharides to form a co-soluble solution, which induces phycoerythrin precipitation under low-temperature conditions, achieving effective separation of phycoerythrin and phycocyanin and obtaining high-purity phycoerythrin. This invention, through variable-parameter aqueous two-phase continuous purification, obtains high-purity phycocyanin while effectively removing both existing and added soluble polysaccharides from the phycobiliprotein-containing solution or biomass extract, avoiding interference from the large molecular size and negative charge of polysaccharides on ultrafiltration purification performance. The prepared phycoerythrin has a PE / PC molar ratio >14, and the phycocyanin has a PC / PE molar ratio >4. Compared with traditional purification methods, this invention effectively solves the high cost and complexity of chromatographic preparation of phycobiliproteins. The method for preparing and purifying phycobiliproteins provided by this invention has the advantages of fewer purification steps, high yield, simple process, and easy scalability. Furthermore, the preparation process is green, environmentally friendly, and low-cost, facilitating industrial production and meeting market demand for high-purity phycobiliproteins, thus possessing significant promotional value.

The invention relates to the field of amino acid preparation, in particular to a preparation method of concerted catalysis high-stability L-aspartic acid, and an efficient synergistic effect is achieved by combining a histidine modified UiO-67 enzyme compound and an amino acid modified ZIF-8 / Pebax-1657 mixed matrix membrane technology. The coordination of histidine and UiO-67 enhances the stability and activity of enzyme, and improves the yield and purity of L-aspartic acid. Meanwhile, due to the high selectivity and permeability of the modified ZIF-8 / Pebax-1657 membrane, the separation efficiency is improved, the L-aspartic acid is effectively purified, the preparation efficiency and the product quality of the L-aspartic acid are improved, the environmental pollution is reduced, and green production and cost control are realized.

The invention relates to a purification method of a monoclonal antibody. According to the purification method disclosed by the invention, the anti-human CCR8 monoclonal antibody is purified by adopting two-step chromatography, the purification steps are few, the sample recovery rate is high, the process is stable, linear amplification can be realized, the high-purity monoclonal antibody can be obtained, residues of host cell protein, DNA and the like in a purified product are few, and the purified monoclonal antibody has relatively high biological activity and biological safety.