6results about How to "Reduced purification steps" patented technology

This invention relates to the field of catalytic dehydrogenation technology for hydrogen storage materials, and discloses a method for dehydrogenating hydrogen storage materials. The method includes: contacting the hydrogen storage material sequentially with a first dehydrogenation catalyst and a second dehydrogenation catalyst under dehydrogenation reaction conditions; wherein the hydrogen storage material includes a hydrogenated N-containing heterocyclic hydrogen storage material and an impurity component; the first dehydrogenation catalyst includes a first support and a first active component supported on the first support, the first active component being Cu; the second dehydrogenation catalyst includes a second support and a second active component supported on the second support, the second active component being at least one of noble metals; the volume ratio of the first dehydrogenation catalyst to the second dehydrogenation catalyst is 1:(1-9). The dehydrogenation method provided by this invention can adapt to hydrogen storage materials containing impurity components, exhibiting high reactivity and good long-term operational stability.

The invention provides a chitosan-dimethyl itaconate conjugate and a synthetic method thereof. The synthetic method comprises the following steps: dissolving CS in deionized water; dissolving DMI in absolute ethyl alcohol; then gradually dropwise adding the dimethyl itaconate solution into the chitosan solution, adding a proper amount of absolute ethyl alcohol, and fully dissolving reactants to form a uniform solution; adding sodium hydroxide to adjust pH; primary amine groups on a CS chain are used as nucleophilic reagents to attack ester carbonyl carbon atoms of DMI, nucleophilic substitution reaction is carried out, and finally amido bonds are formed. Heating reactants under a dark condition, slowly stirring to complete reaction, and ventilating and drying in a drying oven to form a thin film; and eluting the thin film with absolute ethyl alcohol to obtain a final product CS-DMI. The chitosan-dimethyl itaconate synthesized by the method disclosed by the invention has good biocompatibility and anti-inflammatory and antibacterial properties. A strategy and reference are provided for coupling the small molecule medicine to the chitosan, developing a water-soluble high polymer material and applying the small molecule medicine to a medical material.

The invention discloses a method for recovering phosphorus and fluorine from sludge containing phosphorus and fluorine. The method is used for resourceful treatment of the sludge containing phosphorus and fluorine in the phosphorus chemical industry. The method comprises the steps that sludge containing phosphorus and fluorine and experimental water are mixed in proportion to obtain sludge slurry, the sludge slurry is fully stirred so that the sludge slurry can be evenly dispersed, and the experimental water is consistent with field water of phosphorus chemical enterprises and is slag field return water or preparation process water; adjusting the solid content of the sludge slurry to 20%-30%; adding sulfuric acid as a reaction reagent into the sludge slurry, and carrying out a stirring reaction to obtain a mixture; carrying out solid-liquid separation on the mixture through a filtering system, and collecting filtrate containing soluble phosphorus and fluorine and solid-phase residues obtained through separation; and recovering the filtrate for a phosphorus chemical production process to realize phosphorus and fluorine resource recovery, and carrying out subsequent treatment on the solid-phase residue to realize sludge residue reduction.

The invention discloses a method for measuring free amine in amino-terminated liquid nitrile rubber (ATBN) by a gas chromatography technology, which comprises the following steps: weighing an amino-terminated liquid nitrile rubber (ATBN) sample in a centrifuge tube, adding ethanol to precipitate macromolecules to enrich free amine, enabling the free amine to be completely extracted and enriched, and centrifuging to obtain a supernatant; and combining the supernatant enrichment liquid for gas chromatography detection. According to the present invention, the gas chromatography external standard method is adopted to achieve the rapid detection of the free amine in the amino-terminated liquid nitrile rubber (ATBN), and the method has advantages of high repeatability, high test data sensitivity, high accuracy, low instrument operation cost and suitableness for industrial production, and can achieve the purpose of monitoring the reaction raw materials during the ATBN industrial synthesis process.

The invention relates to a method for preparing palmitoyl tetrapeptide-7 through liquid-phase polypeptide synthesis, which comprises the following steps: step 1, obtaining palmitoyl chloride from palmitic acid through acylating chlorination, and then generating Pal-Gly-OH with glycine through substitution reaction; the preparation method comprises the following steps: 1 ', synthesizing N-t-butyloxycarboryl-glutamyl-proline benzyl ester from N-t-butyloxycarboryl-glutamine and proline benzyl ester hydrochloride through dipeptide condensation, and obtaining glutamyl-proline benzyl ester hydrochloride (H-Gln-Pro-OBzl.HCl) through deprotection; step 2, carrying out tripeptide condensation, so as to obtain palmitoyl-glycyl-glutamyl-proline benzyl ester (Pal-Gly-Gln-Pro-OBzl) from Pal-Gly-OH and H-Gln-Pro-OBzl. HCl (hydrogen chloride); step 3, removing a protecting group by virtue of a hydrolysis reaction, so as to prepare palmitoyl-glycyl-glutamyl-proline (Pal-Gly-Gln-Pro-OH); and a step 4 of preparing an active ester Pal-Gly-Gln-Pro-OSu from the Pal-Gly-Gln-Pro-OH by means of ester condensation, and then obtaining the palmitoyl tetrapeptide-7 from the Pal-Gly-Gln-Pro-OSu and arginine by means of tetrapeptide condensation.

This invention discloses a large-scale preparation method for high-purity phycobiliproteins, belonging to the field of purification technology for active ingredients in natural products. This invention utilizes the addition of electronegative polysaccharides or oligosaccharides to form a co-soluble solution, which induces phycoerythrin precipitation under low-temperature conditions, achieving effective separation of phycoerythrin and phycocyanin and obtaining high-purity phycoerythrin. This invention, through variable-parameter aqueous two-phase continuous purification, obtains high-purity phycocyanin while effectively removing both existing and added soluble polysaccharides from the phycobiliprotein-containing solution or biomass extract, avoiding interference from the large molecular size and negative charge of polysaccharides on ultrafiltration purification performance. The prepared phycoerythrin has a PE / PC molar ratio >14, and the phycocyanin has a PC / PE molar ratio >4. Compared with traditional purification methods, this invention effectively solves the high cost and complexity of chromatographic preparation of phycobiliproteins. The method for preparing and purifying phycobiliproteins provided by this invention has the advantages of fewer purification steps, high yield, simple process, and easy scalability. Furthermore, the preparation process is green, environmentally friendly, and low-cost, facilitating industrial production and meeting market demand for high-purity phycobiliproteins, thus possessing significant promotional value.