7 results about "PHA polymerase" patented technology

The present disclosure provides family A DNA polymerases having a strand displacement activity, the variants thereof, and the uses thereof. In one embodiment, the family A DNA polymerase variants exhibit an enhanced activity, salt tolerance, or lower no-template control signal compared to corresponding wild-type family A DNA polymerase.

The invention relates to a T7 RNA polymerase mutant with K1F promoter recognition activity and application of the T7 RNA polymerase mutant in construction of gene circuit logic gate design. The T7 RNA polymerase mutant provided by the invention has RNA polymerase activity based on a K1F promoter, can recognize the corresponding K1F promoter, and keeps orthogonality with a T7 RNAP promoter on the full-length division polymerase variant and on the division polymerase variant with proximity dependency. Compared with the existing K1F RNA polymerase, the K1F RNA polymerase has the advantages of high specificity, enhanced RNA transcriptional activity and the like, the performance is obviously improved, the requirements of current gene circuit multi-path orthogonal regulation and control are met, and an effective candidate enzyme tool is provided for RNA research and application.

Described herein are replicable RNA systems using modified RNA-dependent RNA polymerases having increased trans-amplification activity as compared to the corresponding unmodified polymerases and their use in methods of treating and preventing disease.

A kind of recombinant KOD polymerase.A KOD polymerase mutant, a protein, as follows: for the amino acid residue in the 267, 326, 347, 349, 353, 375, 378, 379, 380, 385, 451, 452, 453, 454, 457, 461, 465, 470, 474, 477, 478, 479, 480, 482, 484, 485, 486, 493, 496, 497, 514, 574, 584, 605, 610, 630, 665, 666, 667, 674, 676, 680, 682, 698, 707, 718, 723 and 729 of KOD DNA polymerase GH78 amino acid sequence at least one of the 48 positions is modified, other amino acid sequences are unchanged, obtain the protein with DNA polymerase activity;Compared with KOD DNA polymerase GH78, the recombinant DNA polymerase shows stronger reaction rate, better catalytic efficiency, better affinity and other advantages in catalysis, improves the reaction rate of DNA polymerase in sequencing, increases reaction read length.

The present application relates to the field of microbiological technology, and particularly relates to a viscous polyhydroxyalkanoate, and its preparation and use. The viscous polyhydroxyalkanoate can be produced by fermenting a Pseudomonas strain containing a mutant of PHA polymerase PhaC61-3, and can be used for the preparation of biodegradable products.

Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with high sensitivity at desirable wavelengths. They contain structural components that modulate the interactions of the analogs with DNA polymerase, thus decreasing photodamage and improving the kinetic and other properties of the analogs in sequencing reactions. Also provided are nucleotide and dye-labeled compounds of the subject analogs, as well as intermediates useful in the preparation of the compounds and analogs. Compositions comprising the compounds, methods of synthesis of the intermediates, compounds, and analogs, and mutant DNA polymerases are also provided.

Disclosed herein is an engineered fusion enzyme composed of a prokaryotic phage RNA polymerase (T7 RNAP) and a viral capping enzyme (NP868R) for the generation of RNA transcripts containing a 5' 7-methylguanylate cap.