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16 results about "Thiolase" patented technology
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Thiolases, also known as acetyl-coenzyme A acetyltransferases (ACAT), are enzymes which convert two units of acetyl-CoA to acetoacetyl CoA in the mevalonate pathway. Thiolases are ubiquitous enzymes that have key roles in many vital biochemical pathways, including the beta oxidation pathway of fatty acid degradation and various biosynthetic pathways. Members of the thiolase family can be divided into two broad categories: degradative thiolases (EC 2.3.1.16) and biosynthetic thiolases (EC 2.3.1.9). These two different types of thiolase are found both in eukaryotes and in prokaryotes: acetoacetyl-CoA thiolase (EC:2.3.1.9) and 3-ketoacyl-CoA thiolase (EC:2.3.1.16). 3-ketoacyl-CoA thiolase (also called thiolase I) has a broad chain-length specificity for its substrates and is involved in degradative pathways such as fatty acid beta-oxidation. Acetoacetyl-CoA thiolase (also called thiolase II) is specific for the thiolysis of acetoacetyl-CoA and involved in biosynthetic pathways such as beta-hydroxybutyric acid synthesis or steroid biogenesis.
Production of fatty alcohols in peroxisome of yarrowia lipolytica
PendingUS20260043055A1Antibody mimetics/scaffoldsMicroorganism based processesHeterologousNucleotide
The invention provides a recombinant Yarrowia lipolytica. The recombinant Yarrowia lipolytica comprises a heterologous polynucleotide encoding a fusion protein. The fusion protein comprises a first amino acid sequence and a second amino acid sequence. The first amino acid may be homologous to the amino acid sequence of 3-ketoacyl CoA thiolase (3KAT), The second amino acid sequence may be homologous to the amino acid sequence of a fatty acyl-CoA reductase (FAR). Also provided is a method for producing one or more fatty alcohols by the recombinant Yarrowia lipolytica and a method for preparing the recombinant Yarrowia lipolytica.
Owner:UNIVERSITY OF DELAWARE
Escherichia coli engineering bacteria with high n-hexanoic acid yield and construction method and application thereof
This invention belongs to the fields of fermentation and genetic engineering technology, and provides a high-yield hexanoic acid-producing engineered *Escherichia coli* strain, its construction method, and its applications. This engineered strain is *Escherichia coli*... E. coli w3110 was the starting strain, overexpressing the acetyl-CoA transferase gene. act and β-ketothiolase gene bktB Overexpression of trans-enoyl-CoA reductase gene ter 3-Hydroxybutyryl-CoA dehydrogenase gene hbd, and 3-hydroxybutyryl coenzyme A dehydratase gene crt Or, simultaneously not expressing the pyruvate formate lyase gene. pflB or lactate dehydrogenase gene ldhA This engineered bacteria can convert glucose into hexanoic acid, a high-value product, with a yield of 4.968 g / L after 18 hours of fermentation. It adopts aerobic fermentation, resulting in rapid cell growth, a short fermentation cycle, and a high acid production rate. The fermentation process is simple, easy to control, and has low production costs, which is conducive to its promotion and application in industrial production.
Owner:SHANXI NORMAL UNIV
Genetically engineered bacteria as a chassis host for high yield terpenoid production
PCT designated stageWO2025217176A1OxidoreductasesAcyltransferasesDiphosphomevalonate decarboxylaseOperon
Described herein are compositions and methods for generating a chassis for high-capacity production of different classes of high-value terpenoids. The chassis is a microorganism host that expresses a first operon comprising a modified ribosomal binding site, a Hmg-CoA reductase (HmgR) polypeptide, a 3-hydroxy-3-methylglutaryl CoA synthase (HmgS) polypeptide, and a β-ketothiolase (PhaA) polypeptide; and a second operon comprising a modified ribosomal binding site, a mevalonate kinase (MvK1) polypeptide, a phospho-mevalonate kinase (MvK2) polypeptide, and a diphosphomevalonate decarboxylase (MvD) polypeptide.
Owner:BOARD OF TRUSTEES OPERATING MICHIGAN STATE UNIV
Serratia marcescens for producing terpenoids and application of serratia marcescens
The invention discloses serratia marcescens for producing terpenoids and application of the serratia marcescens, and belongs to the technical field of bioengineering. According to the invention, part of genes, such as acetoacetyl-coenzyme A thiolase, 3-hydroxy-3-methylglutaryl-coenzyme A synthetase, 3-hydroxy-3-methylglutaryl-coenzyme A reductase and phosphomevalonate decarboxylase, in a haloarchaea MVA pathway are integrated in chromosomes of a serratia marcescens host, and mevalonate kinase is expressed on plasmids, so that the recombinant serratia marcescens is obtained. Meanwhile, competitive genes are knocked out, and a universal terpenoid production strain is constructed. Synthetases of other terpenoids are further introduced into the universal terpenoid production strain and are used for synthesizing more terpenoids.
Owner:XI AN ZHUO HONG CHAO YUAN BIOLOGY SCIENCE & TECHNOLOGY CO LTD
Diatom RNA extraction method and evaluation method for influence of pollutants on diatom gene expression
PendingCN120574824AMicrobiological testing/measurementDNA preparationReference genesAcute toxicity testing
The invention belongs to the technical field of plant molecular biology, and particularly discloses a diatom RNA extraction method and a method for evaluating influence of pollutants on diatom gene expression. The invention firstly provides a diatom RNA extraction method, RNA is extracted by virtue of a cell homogenate physical crushing method and according to an improved TRIzol method, the release amount of RNA is high, and the purity reaches the standard. The invention also provides a method for evaluating the influence of pollutants on gene expression of diatom, which is based on the diatom RNA extraction method, takes Tubulin as a reference gene, takes one or more of iron redox protein, ferrithionein, ketoacyl coenzyme A thiolase, ethanol dehydrogenase, lipoprotein and acetyl coenzyme A carboxylase as target genes, and evaluates the influence of pollutants on gene expression of diatom. The relative expression level of a target gene is detected through qPCR and can be used for evaluating the acute toxicity of pollutants. The method effectively solves the problems that diatom RNA is difficult to extract, the diatom gene expression level is difficult to detect through qPCR, and the molecular mechanism of pollutant action is difficult to evaluate.
Owner:NINGBO UNIV
Genetically engineered bacteria as a chassis host for high yield terpenoid production
PCT designated stageWO2025217176A9OxidoreductasesAcyltransferasesDiphosphomevalonate decarboxylaseOperon
Described herein are compositions and methods for generating a chassis for high-capacity production of different classes of high-value terpenoids. The chassis is a microorganism host that expresses a first operon comprising a modified ribosomal binding site, a Hmg-CoA reductase (HmgR) polypeptide, a 3-hydroxy-3-methylglutaryl CoA synthase (HmgS) polypeptide, and a β-ketothiolase (PhaA) polypeptide; and a second operon comprising a modified ribosomal binding site, a mevalonate kinase (MvK1) polypeptide, a phospho-mevalonate kinase (MvK2) polypeptide, and a diphosphomevalonate decarboxylase (MvD) polypeptide.
Owner:BOARD OF TRUSTEES OPERATING MICHIGAN STATE UNIV
Composition for preventing or treating cancer having resistance to anticancer agents
PCT designated stageWO2026135396A1Organic active ingredientsAntineoplastic agentsChemotherapeutic drugsPharmaceutical drug
The present invention relates to a composition for preventing or treating cancer having resistance to anticancer agents and, more specifically, to a pharmaceutical composition for preventing or treating resistant cancer, comprising a 3-ketoacyl CoA thiolase (ACAA) inhibitor and a carnitine acylcarnitine carrier (CAC) inhibitor and inhibiting the regrowth of cancer having resistance to anticancer agents. It is has been identified that the composition comprising the 3-ketoacyl CoA thiolase (ACAA) inhibitor and the carnitine acylcarnitine carrier (CAC) inhibitor, of the present invention, effectively inhibits the regrowth of cancer cells and tumors having resistance to various anticancer agents, and thus can be effectively used for the prevention or treatment of cancer having resistance to anticancer agents.
Owner:NEW CANCER CURE BIO CO LTD
Kit for catalyzing Friedel-Crafts acylation reaction by enzyme method, application and preparation method
The invention discloses a kit for catalyzing Friedel-Crafts acylation reaction through an enzyme method, application and a preparation method. The kit for catalyzing the Friedel-Crafts acylation reaction by the enzyme method comprises 1) a Friedel-Crafts acylation reaction buffer solution and 2) a thiolase complex MucABC or a functional fragment and a variant thereof. The kit for catalyzing the Friedel-Crafts acylation reaction does not depend on coenzyme A compounds as acyl donors, and can realize regional specific C-acylation modification on various lactam compounds by taking acyl phenyl esters with different chain lengths as the acyl donors under mild conditions; a novel green and efficient biological catalysis tool is provided for Friedel-Crafts acylation reaction, and the method has important value and significance for drug synthesis and chemical production.
Owner:SHENZHEN BAY LAB
Recombinant microorganism for producing adipic acid and hexamethylenediamine and application thereof
The invention relates to the technical field of genetic engineering and biological fermentation, and particularly discloses a recombinant microorganism for producing adipic acid and hexamethylenediamine and application of the recombinant microorganism. Compared with an original strain, the recombinant microorganism disclosed by the invention has the advantages that beta-ketoadipyl coenzyme A thiolase paaJ, 3-hydroxyadipyl coenzyme A dehydrogenase paaH, 2, 3-dehydroadipyl coenzyme A hydratase paaF, trans-2-enoyl coenzyme A reductase ter and acyl coenzyme A thioesterase Acot8 are expressed. The recombinant microorganism can be used for producing adipic acid, and an organic carbon source can be efficiently bioconverted into hexamethylenediamine through mixed culture of the recombinant microorganism capable of producing hexamethylenediamine from adipic acid. The hexamethylenediamine can be directly synthesized by using cheap glucose, glycerol and the like as raw materials, and the production process is green and safe.
Owner:TSINGHUA UNIVERSITY
3-hydroxybutyryl-COA dehydrogenase variants and methods of use
The present disclosure provides thiolases and polypeptide variants of 3-hydroxybutyryl-CoA dehydrogenase, nucleic acids encoding the same, vectors comprising the nucleic acids, and cells comprising the polypeptide variants and / or thiolase, the nucleic acids, and / or the vectors. The present disclosure also provides methods of making and using the same, including methods for culturing cells, and for the production of various products, including 3-hydroxybutyryl-CoA (3-HB-CoA), 3-hydroxybutyraldehyde (3-HBal), 3-hydroxybutyrate (3-HB), 1,3-butanediol (1,3-BDO), and esters and amides thereof, and products made from any of these.
Owner:AGAIN TECHNOLOGY HOLDINGS INC
Enzymatic synthesis of immunosuppressant mycophenolic acid and its derivatives
The present invention provides an enzymatic synthesis method for the immunosuppressant mycophenolic acid and its derivatives. This method utilizes a multi-enzyme, multi-cycle cascade reaction strategy to catalyze the conversion of the substrates 4-dienodecanoic acid-3-hydroxy-5-hydroxymethyl-6-methylphthalide or 4-dienodecanoic acid-3,5-dihydroxy-6-methylphthalide into mycophenolic acid and its derivatives. The multi-enzyme combination comprises an acyl-CoA ligase, an FAD-dependent oxidase, a 2,4-dienoyl-CoA reductase, an enoyl-CoA isomerase, a bifunctional enzyme with hydratase and dehydrogenase functions, and a thiolase; in addition, a CoA hydrolase and an oxygen-methyltransferase. This method, for the first time, achieves the in vitro enzymatic synthesis of (M)FDHMP-3C to MPA, fully elucidating the mycophenolic acid oxidative degradation synthesis pathway and the catalytic sequence of the catalytic enzymes.
Owner:SHANDONG UNIV
Microorganism with improved production level of p-coumaric acid or p-coumaric acid derivative, and construction method and application thereof
The application discloses a kind of p-coumaric acid or p-coumaric acid derivative production level improved microorganism and its construction method and application, comprising: the genetic target point related to p-coumaric acid metabolic pathway of microorganism is reformed, target point is 4-coumaroyl coenzyme A ligase, hydroxycinnamoyl coenzyme A hydratase lyase, hydroxyacyl coenzyme A dehydrogenase, beta-ketoacyl-coa thiolase, 3- (aryl) propenoate reductase one or more than two combinations, reformation is removed the partial activity or all activity of p-coumaric acid metabolic pathway related genetic target point.The application reduces the degree of degradation of p-coumaric acid by microorganism by reformation p-coumaric acid metabolic pathway related genetic target point, to improve the yield and / or production efficiency of p-coumaric acid and p-coumaric acid derivative produced by microorganism.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Recombinant saccharomyces cerevisiae strain for producing cannabinoid phenolic acid at high yield by using sugar as well as construction method and application of recombinant saccharomyces cerevisiae strain
PendingCN121873991AAvoid the bottleneck of caproic acid toxicityFungiHydrolasesEnzyme GeneGeranyl pyrophosphate
The invention relates to the technical field of metabolic engineering, in particular to a recombinant saccharomyces cerevisiae strain for producing cannabinoid phenolic acid at high yield by using sugar as well as a construction method and application of the recombinant saccharomyces cerevisiae strain. According to the invention, firstly, reverse beta-oxidation pathway genes ReBktB, CnPaaH1, CaCrt and TdTer are introduced, so that the problem that cannabinoid phenolic acid biosynthesis depends on a hexanoic acid substrate is solved; and then the gene GAL80 of the GAL4 inhibitory protein is knocked out, and the GAL4 is over-expressed, so that the overall expression level of the pathway is up-regulated. Then, a mercaptase gene Ckth1A and a hydroxyacyl coenzyme A dehydrogenase gene Ckhbd1 are introduced, so that the supply of the hexanoyl coenzyme A is improved. Furthermore, the endogenous farnesyl pyrophosphate synthetase gene ERG20 is down-regulated, and the consumption of geranyl pyrophosphate by a branch path is reduced. Finally, the expression level of part of genes in saccharomyces cerevisiae cells is regulated and controlled through gene editing, and the purposes of synthesizing cannabinoid phenolic acid from the beginning by using saccharomyces cerevisiae and taking sugar as a substrate and improving the yield of cannabinoid phenolic acid are achieved.
Owner:SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI +1
A recombinant strain for producing squalene, a construction method thereof and applications thereof
The present invention discloses a recombinant strain for producing squalene, a construction method thereof and an application thereof. The construction method includes: heterologously expressing the cDNA of each gene of the squalene synthesis pathway derived from filamentous fungi in yeast. The genes of the squalene synthesis pathway include: mevalonate-5-phosphate kinase gene, squalene synthase gene, acetoacetyl-CoA thiolase gene, mevalonate kinase gene, hydroxymethylglutaryl-CoA synthase gene, mevalonate pyrophosphate decarboxylase gene, farnesyl pyrophosphate synthase gene, isopentenyl pyrophosphate:dimethylallyl pyrophosphate isomerase gene, and truncated hydroxymethylglutaryl-CoA reductase gene; the filamentous fungus is Sarocladium oryzae or Stibella sp.1056; the squalene synthesis pathway excavated by the present invention can complete the efficient de novo synthesis of squalene and improve the squalene yield.
Owner:THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
Production of medium chain length 3-hydroxyacyl acids
The present disclosure uses a combination of transcriptomics and genetics to demonstrate that P. putida PhaG is likely a 3-hydroxyacyl-ACP thiolase rather than a 3-hydroxyacyl-ACP:CoA transacylase. Deletion of two 3-hydroxyacyl CoA synthases results in the abolishment of PHAs as a product and leads to the accumulation of free medium chain length 3-hydroxyacyl acids as products into the culture supernatant under nitrogen starvation conditions. The present disclosure demonstrates a biological route to the production of 3-hydroxyacyl acids for use as industrial chemicals.
Owner:UT BATTELLE LLC
A method for metabolically engineering brewer's yeast to produce limonene and its derivatives
ActiveCN116515658BPolypeptide with localisation/targeting motifFungiIsopentenyl pyrophosphateOxidative enzyme
This invention discloses a method for metabolically engineering Saccharomyces cerevisiae to produce limonene and its derivatives, belonging to the field of biotechnology. The recombinant Saccharomyces cerevisiae uses a Saccharomyces cerevisiae strain with integrated cytochrome P450 oxidase and CPR genes from Salvia miltiorrhiza as the chassis cell. The following genes, with the nucleotide sequence ePTS linked to the 3' end of each gene, are overexpressed: acetyl-CoA thiolase, HMG-CoA synthase, HMG-CoA reductase (tHMGR), mevalonate kinase, phosphate mevalonate kinase, diphosphate mevalonate kinase, isopentenyl pyrophosphate isomerase, and farnesyl pyrophosphate synthase, as well as a truncated limonene synthase from Spearmint. After 120 h of shake-flask fermentation, the recombinant strain HY-PER-03 produced 86.74 mg / L of limonene, while S. cerevisiae HY-PA-01 produced 4.42 mg / L of perillic acid.
Owner:JIANGNAN UNIV