33 results about "Acetoacetyl-CoA" patented technology

The invention describes a process for preparing acetone starting from acetyl-coenzyme A comprising process steps A. enzymatic conversion of acetyl-CoA into acetoacetyl-CoA B. enzymatic conversion of acetoacetyl-CoA into acetoacetate and CoA and C. decarboxylation of acetoacetate to acetone and CO₂, which is characterized in that the coenzyme A is not transferred in process step B to an acceptor molecule. In addition, process step B is surprisingly catalysed by enzymes of the classes of acyl-CoA thioesterase, acyl-CoA synthetase or acyl-CoA thiokinase.

A completely novel metabolic pathway is concerned, because the enzymatic hydrolysis of acetoacetyl-CoA without simultaneous transfer of CoA to a receptor molecule has never previously been described for any microbial enzyme.

The invention provides a method for constructing a gene engineering strain to enhance the resistance of a 1, 3-propanediol (PDO) production strain, which belongs to the technical field of biochemical engineering and comprises the step of: introducing Beta-ketothiolase PhbA, NADPH-dependent acetoacetyl CoA, PhbB and polyhydroxyalkanoates synthases, PhbC gene to a wild bacteria which can produce PDO to accumulate high concentration of PHB while the bacterial strain produces PDO so as obviously improve the tolerance of the bacterial strain to glycerol and 3-hydroxypropionaldehyde; the method can also be used to co-produce PHB and PDO. The method has the advantages that the constructed gene engineering strain improves the resistance of the bacterial strain to the high concentration of glycedrol and the intermediate product 3-hydroxypropionaldehyde and reduces the abnormal fermentation in industrial production; in addition, high concentration of PHB can be accumulated while PDO is produced, and PHB can be used as a separated product, thereby promoting the added valve of products and the utilization rate of materials and reducing the production cost.

The invention discloses a technique for biotransforming CO2 into isopropyl alcohol by utilizing blue algae, and provides the following six methods for preparing recombined blue algae: (1) leading a CoA transferase gene, an acetoacetate decarboxylase gene and an alcohol dehydrogenase gene into the blue algae; (2) leading the CoA transferase gene and the acetoacetate decarboxylase gene into the blue algae; (3) leading a coded gene of the alcohol dehydrogenase into the recombined blue algae in (2); (4) leading an acetyl-CoA acetyltransferase gene, an acetoacetyl-CoA transferase gene, the acetoacetate decarboxylase gene and the alcohol dehydrogenase gene into the blue algae; (5) leading the acetyl CoA acetyltransferase gene, the acetoacetyl-CoA transferase gene and the acetoacetate decarboxylase gene into the blue algae; and (6) leading the alcohol dehydrogenase gene into the recombined algae generating acetone in (5). The technique provided by the invention is one of ideal paths for realizing the continuous and healthy development of renewable and clean energy by utilizing the recombined algae to produce acetone and the isopropyl alcohol.

The expression vector includes: the nucleic acids coding for the polypeptides forming a polypeptide complex having an enzyme activity allowing acetoacetyl-CoA to be converted to acetoacetate; optionally, at least one nucleic acid coding for a polypeptide having an enzyme activity allowing acetoacetate to be converted to acetone; and at least one nucleic acid coding for a polypeptide having an enzyme activity allowing acetone to be converted to isopropanol; the expression of the nucleic acids being controlled by a single constitutive promoter located upstream of the abovementioned nucleic acids.

The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3HB and LA via microbial fermentation with the use of a sugar as a starting material.

The following is provided: a method for producing a polyester copolymer consisting of 3-hydroxybutyrate and lactate, which comprises the steps of:

• • (1) culturing a recombinant microorganism having a protein capable of catalyzing a reaction of transferring CoA to propionic acid and/or lactate, a protein capable of catalyzing a reaction of forming acetoacetyl-CoA from two acetyl-CoA molecules, a protein capable of catalyzing a reaction of acetoacetyl-CoA reduction, and a protein capable of catalyzing a reaction of polyhydroxyalkanoate synthesis consisting of the following amino acid sequence (a) or (b) in a medium containing a carbon source:
  • (a) an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of at least one of the amino acids at positions 130, 325, 477, and 481 with different amino acid(s); or
  • (b) an amino acid sequence derived from the protein specified in (a) by additional deletion or substitution of at least one amino acid other than the amino acids at positions 130, 325, 477, and 481 or by insertion of at least one amino acid residue; and
  • (2) collecting the polyester copolymer from the culture product obtained in step (1). According to the production method of the present invention, a polyester copolymer consisting of 3HB and LA can be efficiently produced using an inexpensive carbon source as a starting material, and thus the production cost of a biodegradable plastic can be reduced.

A transformant capable of producing isopropanol which is constructed by transferring the following genes (a) to (d) into a coryneform bacterium:

• (a) an exogenous gene which encodes an enzyme having acetyl-CoA acetyltransferase activity;
• (b) an exogenous gene which encodes an enzyme having acetoacetyl CoA: acetate CoA-transferase activity;
• (c) an exogenous gene which encodes an enzyme having acetoacetate decarboxylase activity; and
• (d) an exogenous gene which encodes an enzyme having isopropanol dehydrogenase activity.

A method of manufacturing a butanediol includes a step of culturing a microbe that contains a gene that codes acetoacetyl-CoA synthase that catalyzes a reaction that irreversibly produces acetoacetyl-CoA from acetyl-CoA and malonyl-CoA, acetoacetyl-CoA reductase that catalyzes a reaction that produces 3-hydroxybutyryl-CoA from acetoacetyl-CoA, and an enzyme that catalyzes a reaction for producing a butanediol from 3-hydroxybutyryl-CoA.

The invention provides microorganisms and methods for the production of polyhydroxybutyrate (PHB) from gaseous substrates. In particular, the invention provides a non-naturally occurring Wood-Ljungdahl microorganism comprising (a) an enzyme that converts acetyl-CoA to acetoacetyl-CoA, (b) an enzyme that converts acetoacetyl-CoA to 3-hydroxybutyryl-CoA, and (c) an enzyme that converts 3-hydroxybutyryl-CoA to polyhydroxybutyrate, and methods related thereto.

The invention relates to 3-hydroxyl-3-mathylglutaryl-CoA reductase albumen encoding sequence of barbadosnut, which belongs to the technical field of molecular biology and gene engineering. The DNA molecule separation comprises the following steps: the nucleotide sequence of polypeptide with barbadosnutJcHMGRs albumen activity is encoded, and the nucleotide sequence has a homology of at least 70 percent with the nucleotide sequence with nucleotide ranging from bit 81-1832 in SEQ ID NO.1; the nucleotide sequence can be hybridized with the nucleotide sequence with nucleotide ranging from bit 81-1832 in SEQ ID NO.2 at 40-55 DEG C. The invention is an enzyme, namely, 3-hydroxyl-3-mathylglutaryl-CoA generated by catalyzing acetyl-CoA and acetoacetyl coenzyme A. The enzyme is favorable for reducing the content of phorbol ester or the prosoma thereof in barbadosnut and greatly helpful for cultivating quality variety of aseptic barbadosnut.

A method of manufacturing 1,4-butanediol through acetyl-CoA, acetoacetyl-CoA, 3-hydroxybutyryl-CoA, crotonyl-CoA, and 4-hydroxybutyryl-CoA by using a microbe and/or a culture thereof, wherein the microbe in the manufacturing method for 1,4-butanediol includes any one of genes among (a) a gene that has a base sequence of sequence number 1, (b) a gene that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and (c) a gene that hybridizes with a gene that has a base sequence complementary with a gene that has a base sequence described in sequence number 1 on a stringent condition, and includes any one or more genes among (d) genes that have base sequences of sequence numbers 2 to 9, (e) genes that have base sequences such that one or more bases are deleted, substituted, or added in base sequences of sequence numbers 2 to 9, wherein the genes have base sequences with an identity greater than or equal to 90% with respect to original base sequences thereof, and (f) genes that hybridize with genes that have base sequences complementary with genes that have base sequences of sequence numbers 2 to 9 on a stringent condition.