5 results about "Malonyl Coenzyme A" patented technology

The application discloses a blood-activating herb naringenin synthase gene GlCHS, a product coded by the gene and application thereof, and belongs to the field of genetic engineering. The blood-activating herb naringenin synthase gene GlCHS is cloned from a blood-activating herb, the nucleotide sequence of the gene is shown as SEQ ID No. 1, and the amino acid sequence of the product coded by the gene is shown as SEQ ID No. 2. The blood-activating herb naringenin synthase is expressed heterogeneously in a host cell, and the enzyme has the function of catalyzing naringenin to be generated by taking p-coumaroyl coenzyme A and malonyl coenzyme A as substrates. By using the application, the content of naringenin in the blood-activating herb can be improved and naringenin can be synthesized in vitro in a medicinal plant by using genetic engineering technology.

The application provides a genetically engineered bacterium with high yield of dihydroquercetin, a preparation method and application thereof, relates to the technical field of genetic engineering, and comprises a malonyl coenzyme A synthesis module, a tyrosine deaminase expression module, a naringenin synthesis module and a dihydroquercetin post-modification module. The enzymatic property of the fusion protein is increased by about 50% of the enzyme activity before fusion, the activity of converting naringenin into dihydroquercetin is greatly improved, and finally the yield of dihydroquercetin expressed in cyanobacteria is greatly improved. Meanwhile, BauA, MCR-C, Sam8, 4CL, CHS, CHI and expression fusion protein N33'H are expressed in cyanobacteria. The yield of dihydroquercetin is greatly increased under seawater culture conditions. The technical problem that the yield of biosynthetic dihydroquercetin is low and cannot meet the requirements of industrial application in the prior art is solved.

The invention relates to a recombinant engineering bacterium for producing a pyrone derivative as well as a construction method and application of the recombinant engineering bacterium, and belongs to the technical field of biological engineering. By optimizing the metabolic flux of malonyl coenzyme A and 3-hydroxy propionyl coenzyme A, a novel pyrone derivative 4-hydroxy-6-hydroxyethyl-2-pyrone (HHEP) is obtained, a complete heterologous synthesis path of the pyrone derivative 4-hydroxy-6-hydroxyethyl-2-pyrone (HHEP) is constructed in recombinant engineering bacteria, efficient de novo synthesis of the HHEP is realized, the yield of the HHEP is increased, and the yield of the HHEP is increased. Glucose is used as a single carbon source, a toxic reagent or a complex purification process is not needed, and the shake flask yield of HHEP within 120 hours reaches 3.6 g / L. The HHEP has antibacterial activity, and a breakthrough technical scheme is provided for low-cost and sustainable manufacturing of a novel antibacterial agent. Meanwhile, the recombinant engineering bacterium provides a breakthrough technical scheme for low-cost and sustainable manufacturing of the HHEP.

The invention belongs to the technical field of gene engineering, and particularly relates to a chaenomeles speciosa chalcone synthase gene CsCHS as well as a coding product and application thereof. The invention provides a chaenomeles speciosa chalcone synthase gene CsCHS, a protein product coded by the chaenomeles speciosa chalcone synthase gene CsCHS, and an application of the chaenomeles speciosa chalcone synthase gene CsCHS. The three kinds of chaenomeles speciosa CsCHS1, CsCHS2 and CsCHS3 genes are obtained through cloning, and protein products of the three kinds of chaenomeles speciosa CsCHS1, CsCHS2 and CsCHS3 genes are successfully expressed and purified in a pronucleus system. An in-vitro enzymatic experiment shows that the obtained CsCHS protein can be catalyzed by taking 4-coumaroyl coenzyme A and malonyl coenzyme A as substrates to generate naringenin, and the CsCHS protein is proved to have typical chalcone synthase catalytic activity.

The invention relates to the field of plant molecular biology, in particular to a pinus massoniana pinocembrin synthase gene, a product and application. The invention discloses a pinus massoniana pinocembrin synthase gene. The nucleotide sequence of the pinus massoniana pinocembrin synthase gene is as shown in SEQ ID NO. 1. The pinus massoniana pinocembrin synthase gene is cloned from the pinus massoniana pinocembrin synthase gene for the first time, the research blank on application of the pinus massoniana pinocembrin synthase gene in the prior art is filled up, and theoretical and basic support is provided for related research on disease resistance of the pinus massoniana pinocembrin. Besides, the inventor obtains a recombinant vector and an engineering bacterium through genetic recombination of the pinus massoniana pinocembrin synthase, the pinus massoniana pinocembrin synthase is obtained through successful purification, the synthase can catalyze condensation reaction of cinnamyl coenzyme a and three malonyl coenzyme a to synthesize pinocembrin in an in-vitro enzyme activity experiment, and the pinus massoniana pinocembrin synthase has important significance on pine trees to resist pine wood nematode diseases.