15 results about "Streptomyces hygroscopicus" patented technology

The invention provides a streptomyces hygroscopicus engineering strain as well as construction and application thereof, and relates to the technical field of biology, the engineering strain takes streptomyces hygroscopicus CL-1 as a starting host and is obtained by heterologous expression of an epsilon-polylysine synthetase encoding gene pls, and the nucleotide sequence of the epsilon-polylysine synthetase encoding gene pls is shown as SEQ ID NO.1. Streptomyces hygroscopicus CL-1 is used as a host, the epsilon-polylysine synthetase coding gene derived from streptomyces albus is subjected to heterologous expression, the obtained recombinant strain has high epsilon-polylysine production capacity, and under the optimized fermentation condition, the yield of epsilon-polylysine is 0.87 g / L, and industrial scale production requirements are met. Meanwhile, the broad-spectrum antibacterial activity to various plant pathogenic fungi and common bacteria, including colletotrichum gloeosporioides, fusarium oxysporum, phytophthora capsici and the like, is also remarkably improved. Therefore, the streptomyces hygroscopicus has important application in the aspects of production of epsilon-polylysine, prevention and control of pathogenic microorganisms, preservation and the like, and the industrial and agricultural utilization potential of the streptomyces hygroscopicus is expanded.

The present invention relates to a method for preparing a fermentation liquid of Streptomyces hygroscopicus and its application. The method for preparing a fermentation liquid of Streptomyces hygroscopicus comprises the following steps: inoculating a fermentation medium with a strain named Streptomyces hygroscopicus JN3-4, and fermenting and culturing, wherein the deposit number of Streptomyces hygroscopicus JN3-4 is CGMCC No. 27649. The fermentation liquid prepared by the above method can be used for biological control of pathogenic bacteria such as Aggregative Small Hole Shell Fungus, Red Bean Anthracnose Bacteria, Red Pine Blight Bacteria, Corn Big Spot Bacteria, Tobacco Target Spot Bacteria, Rice Bakanae Bacteria, Melon Vine Cut Bacteria, Pepper Anthracnose Bacteria, Pear Boy's Disk Fruit Rot Bacteria, Cucumber Sclerotinia Bacteria, Blueberry Canker Bacteria, Larch Blight Bacteria, etc., and has the advantages of good biological control effect, wide antibacterial spectrum, wide application range, etc.

The invention discloses streptomyces hygroscopicus ascomycetes subspecies and an obtaining method thereof. Regulating genes fkbN, fkbR1 and fkbR2 on an ascomycin biosynthesis gene cluster of the streptomyces hygroscopicus ascomycetes subspecies ATCC 14891 and a gene fkbS participating in synthesis of an ascomycin precursor ethyl malonyl coenzyme A are subjected to biosynthesis to obtain the streptomyces hygroscopicus ascomycetes subspecies. And carrying out overexpression on a regulatory gene SH03686 of a leucine response regulatory protein family (Lrp) outside the gene cluster, and screening an ascomycin high-yield strain. According to the invention, after combined overexpression of genes fkbS and fkbN and overexpression of a gene fkbR2 are carried out by a strain SN9 and a strain R2-17 in starting bacteria, the transcriptional level of regulatory genes (fkbN, fkbR2) on an ascomycin biosynthesis gene cluster and related genes participating in synthesis of precursors ethyl malonyl coenzyme A (fkbS, fkbE), piperidine acid (fkbL) and DHCHC (fkbO) are improved, and finally, the ascomycin yield is improved.

The present invention provides a composite fungal herbicide, belonging to the technical field of microbial herbicides. The composite fungal herbicide comprises active herbicidal ingredients: Streptomyces hygroscopicus M22 and Curvularia lunata B6. The combined use of Streptomyces hygroscopicus M22 and Curvularia lunata B6 creates a synergistic effect, broadens the weed control spectrum, and enhances the control of field weeds. The weed control effect is superior to that of a single agent, and is superior to other microbial herbicides. Furthermore, the composite herbicide is highly safe for crops and does not cause phytotoxicity.

The invention belongs to the technical field of bioengineering, and provides an engineering bacterium for producing an amino sugar intermediate as well as a construction method and application thereof, the engineering bacterium for producing the amino sugar intermediate inhibits synthesis of validamycin by knocking out genes on the basis of streptomyces hygroscopicus, and valilone synthetase is over-expressed. The engineering bacterium disclosed by the invention realizes high-yield effective alcohol amine through multiple modifications, and the yield reaches 513.8 mg / L.

The invention discloses an application of an lprR gene in regulating and controlling the predation capability of stenotrophomonas maltophilia. The invention provides a substance for inhibiting or reducing or down-regulating the content or activity of LprR protein, or a substance for inhibiting or reducing or down-regulating the expression of an LprR protein coding gene, and any one of the following applications: 1) improving the predatory ability of stenotrophomonas maltophilia; experiments prove that after the lprR gene is knocked out, the predatory ability of the mutant under the hungry condition is remarkably improved, the predatory ability of a complementary strain basically recovers to be consistent with that of a wild type, and in an NYG culture medium, the predatory ability of the mutant, the predatory ability of the complementary strain and the predatory ability of the wild type are not remarkably different.

This invention discloses an inhibitor for controlling fall armyworm and its application, relating to the field of insect control technology. The inhibitor uses rapamycin or AZD-8055 as the main active ingredient and DMSO or water as the solvent. Rapamycin is a macrolide immunosuppressant produced by *Streptomyces hygroscopicus*, and AZD-8055 is an ATP-competitive TOR kinase inhibitor. The effective working concentration of rapamycin is 0.1 μM to 50 μM; the effective working concentration of AZD-8055 is 0.1 μM to 50 μM. The inhibitor causes morphological changes or death in the fall armyworm ovarian cell line Sf9. The inhibitor provided by this invention uses rapamycin or AZD-8055 as the active ingredient, targets the insect TOR signaling pathway, has no cross-resistance with existing chemical pesticides and Bt protein, and can effectively respond to resistant populations, solving the problem of pesticide resistance in fall armyworm.

The invention discloses genetically engineered streptomyces as well as a construction method and application thereof. According to the present invention, an aveA3 fragment in a Streptomyces avermitilis gene cluster capable of generating an ivermectin B1b compound is replaced with a milA3 fragment in a Streptomyces hygroscopicus gene cluster so as to obtain the gene engineering Streptomyces, such as Streptomyces avermitilis HU501-M, and the gene engineering Streptomyces HU501-M can be used to produce the ivermectin B1b compound, such that the gene engineering Streptomyces HU501-M can be used to produce the ivermectin B1b compound, and the ivermectin B1b compound can be used to produce the ivermectin B1b compound, and the ivermectin B1b compound can be used to produce the ivermectin B1b compound, and can be used to produce the ivermectin B1b compound. The constructed genetically engineered streptomyces can be used for preparing milbemycin D, and the compound has efficient insecticidal, acaricidal and antiparasitic activity, is high in safety to human beings and animals, and is environment-friendly. The main fermentation product of the genetically engineered streptomyces is milbemycins D, and the genetically engineered streptomyces has a good industrial development prospect.

The invention belongs to the technical field of epsilon-polylysine production, and particularly relates to a screening culture method and application of tolerant streptomyces hygroscopicus. The screening culture method of the tolerant streptomyces hygroscopicus comprises the following steps: subculturing streptomyces hygroscopicus engineering bacteria in a culture medium taking straw cellulose hydrolysate as a carbon source for at least 30 generations to obtain primary streptomyces hygroscopicus. The primary streptomyces hygroscopicus is subcultured for 10-25 generations in a fermentation culture medium with the epsilon-polylysine concentration being 0.5 g / L-5g / L and straw cellulose hydrolysate as a carbon source, and the tolerant streptomyces hygroscopicus is obtained. Wherein the tolerance means that epsilon-polylysine is produced while an inhibitor is resisted. The straw cellulose hydrolysate contains an inhibitor. According to the screening culture method of the tolerant streptomyces hygroscopicus, disclosed by the invention, the yield of epsilon-polylysine in virus-free cellulose hydrolysate can be up to 1.35 g / L at most, and the yield of epsilon-polylysine can be up to 30.57 g / L at most under the condition of fed-batch fermentation in a 5L fermentation tank.

The application discloses a corn stalk fermentation product, which is prepared by solid fermentation and leaching with corn stalk as a substrate, and by simultaneously introducing Streptomyces hygroscopicus B04 and Guizhou Trichoderma NJAU4742 as functional microorganisms; the strain of Streptomyces hygroscopicus B04 has a preservation number of CGMCC NO.9918; and the strain of Guizhou Trichoderma NJAU4742 has a preservation number of CGMCC No.12166. In the present application, corn stalk is used as a substrate, and functional substances required for growth are generated by decomposing the stalk in the co-fermentation process, so that the fermentation process is better performed, and the biomass of Streptomyces hygroscopicus B04 is significantly increased after fermentation, and the order of magnitude reaches 10 9 million / g of stalk. Meanwhile, the metabolites can significantly promote the growth of plants, and the plant biomass is significantly increased. The application also discloses application of the corn stalk fermentation product in promoting the growth of plants or in preparing a fertilizer or preparation for promoting the growth of plants.

The invention belongs to the technical field of bioengineering, and provides an engineering bacterium for producing an amino sugar intermediate as well as a construction method and application thereof, the engineering bacterium for producing the amino sugar intermediate inhibits synthesis of validamycin by knocking out genes on the basis of streptomyces hygroscopicus, and valilone synthetase is over-expressed. The engineering bacterium disclosed by the invention realizes high-yield effective alcohol amine through multiple modifications, and the yield reaches 318.8 mg / L.

The invention discloses a streptomyces hygroscopicus sh-37 strain for preventing and treating citrus canker and application of the streptomyces hygroscopicus sh-37 strain. The sh-37 strain is preserved in the Guangdong Microbial Culture Collection Center on April 26, 2024, and the preservation number is GDMCC No: 64572. Researches show that the sh-37 strain single colony is round and light purple gray, the edge is neat and free of wrinkles, and aerial hyphae are white and not developed; meanwhile, the sh-37 strain has a relatively strong bacteriostatic action on citrus canker pathogens, has an obvious control effect on citrus canker, and can provide a new effective control means and technical support for comprehensive control of citrus canker.

The invention discloses genetically engineered streptomyces as well as a construction method and application thereof. According to the present invention, with the application of the CRISPR-Cas9 gene editing technology, the aveA3 fragment in the Streptomyces avermitilis gene cluster capable of generating the ivermectin B1b compound is replaced with the milA3 fragment in the Streptomyces hygroscopicus gene cluster so as to obtain the gene engineering Streptomyces, such as Streptomyces avermitilis HU501-M, and the gene engineering Streptomyces HU501-M can be used to produce the ivermectin B1b compound, the constructed genetically engineered streptomyces can be used for preparing milbemycin D, and the compound has efficient insecticidal, acaricidal and antiparasitic activity, is high in safety to human beings and animals, and is environment-friendly. The main fermentation product of the genetically engineered streptomyces is milbemycins D, and the genetically engineered streptomyces has a good industrial development prospect.

The invention belongs to the technical field of biopesticides, and provides a complex microbial inoculant and an application thereof in promoting growth of zizania aquatica, the complex microbial inoculant is composed of Ustilago esculenta G1 and Streptomyces hygroscopicus Sh-2, the strain preservation number of the Ustilago esculenta G1 is CGMCC (China General Microbiological Culture Collection Center) No.40037, the preservation number of the Streptomyces hygroscopicus Sh-2 is CGMCC No.33980, the collection number of the Streptomyces hygroscopicus Sh-2 is CGMCC No.33980, the collection number of the Streptomyces hygroscopicus Sh-2 is CGMCC No.33980, and the collection number of the Streptomyces hygroscopicus Sh-2 is CGMCC No.33980. When the complex microbial inoculant is applied to zizania aquatica cultivation, the biodiversity of the zizania aquatica growth environment can be enriched, the utilization rate of the fertilizer is increased, and then the yield of zizania aquatica is increased.

The invention relates to the technical field of microbial genetic engineering, in particular to a milbemycins high-yield strain as well as a construction method and application thereof, and the strain is constructed by introducing an integrated CRISPRi plasmid capable of dynamically inhibiting target gene transcription on the basis of streptomyces hygroscopicus SIPI-054. The integrated CRISPRi plasmid comprises a nucleotide sequence for expressing dCas9 protein activity, a time sequence type promoter for driving dCas9 protein expression, sgRNA for a target gene and a constitutive promoter for driving sgRNA transcription, the target gene is a primary metabolism key node gene, and the time sequence type promoter drives dCas9 protein dynamic expression. According to the invention, an endogenous quorum sensing system SbbR / SbbA of streptomyces hygroscopicus SIPI-054 is coupled with dCas9-mediated CRISPRi, and a sequential starting element sbbAp is used for driving dCas9 expression, so that an engineering strain autonomously and dynamically inhibits key nodes of a primary metabolic pathway in a cell density-dependent manner to maintain dynamic balance of primary metabolism and secondary metabolism, and the expression of the dCas9 is promoted. Further, the fermentation level of milbemycins is improved.