12 results about "Streptomyces avermitilis" patented technology

The invention discloses a 13-hydroxyglucopiericidin A heterologous expression engineering strain, a construction method of the 13-hydroxyglucopiericidin A heterologous expression engineering strain and an application of the 13-hydroxyglucopiericidin A heterologous expression engineering strain. According to the engineering strain, Streptomyces avicularis SUKA17 is taken as a host, and heterologous synthesis of the chalicimycin glycoside, especially 13OH-GPA, is realized by introducing a biological synthesis gene cluster of the chalicimycin glycoside; the bacterial strain is classified and named as Streptomyces sp. SK-SA23E, and the preservation number of the bacterial strain is GDMCC NO.67271. The invention further discloses a preparation method of the bacterial strain. According to the invention, the efficient heterologous expression of the biological synthesis gene cluster of the chalcone glycoside is realized for the first time, the yield of the 13OH-GPA in the engineering strain is increased by 2.8 times compared with that of the original production strain, the relative content of the 13OH-GPA in the chalcone glycoside is increased to 48.1% from 23.6%, and the method is suitable for large-scale preparation of the 13OH-GPA.

The invention belongs to the technical field of genetic engineering and metabolic engineering, and particularly relates to a streptomyces avermitilis double promoter and application thereof. The double promoter ermEp *-P7983 provided by the invention is formed by connecting a promoter ermEp * and a promoter P7983 in series, and the nucleotide sequence of the double promoter ermEp *-P7983 is as shown in SEQ ID NO. 2. The double promoter has higher activity, and can significantly improve the expression level of a target gene in abamectin and increase the yield of a target metabolite, so that the double promoter can be used for constructing a recombinant plasmid for highly expressing the target gene and a streptomyces avermitilis recombinant bacterium, and has broad application prospects. And the constructed streptomyces avermitilis recombinant strain shows the activity of efficiently synthesizing a target product in different culture media, and has stronger adaptability.

The application discloses a strain of Streptomyces clyindrosporus 11-7 and application thereof, and belongs to the technical field of microorganisms. The strain of Streptomyces clyindrosporus 11-7 disclosed by the application has a preservation number of CCTCC NO: M 2025222. The strain of Streptomyces clyindrosporus 11-7 disclosed by the application has the best inhibitory activity on Colletotrichum chlorophyti, Colletotrichum plurivorum and Rigidoporus lignosus, and the inhibitory rates are 96.75%, 95.89% and 92.94% respectively. The volatile substances produced by the strain of Streptomyces clyindrosporus 11-7 have inhibitory effects on various pathogenic bacteria. The strain of Streptomyces clyindrosporus 11-7 can fix nitrogen, produce ammonia and produce indole acetic acid.

The present application relates to a kind of high-yield abamectin B2 Streptomyces avermitilis AVE-16 and its preparation method, the Streptomyces avermitilis AVE-16 is preserved in China Microbial Culture Collection Center, and the preservation number is CGMCC No.38200.The present application uses Streptomyces avermitilis XB-12 as starting strain, uses ethyl methanesulfonate and atmospheric pressure plasma combined mutagenesis technology to obtain a Streptomyces avermitilis AVE-16, and the yield of abamectin B2a is increased by 145% compared with starting strain, and the total yield of abamectin (B1a+B2a) is increased by 44% compared with starting strain.

The invention belongs to the technical field of microorganisms, and discloses streptomyces yellowish-dark color and application thereof in agriculture. The invention provides a strain of streptomyces yellowish-dark color, the streptomyces yellowish-dark color is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20251791. The streptomyces yellowish-dark color is fast in growth and reproduction, strong in adaptive capacity, nontoxic and harmless to people and livestock, and suitable for agricultural production. In the aspect of field biological prevention and control, the streptomyces yellowish-dark color thallus and the fermentation liquor have an excellent prevention effect on tobacco bacterial wilt, and are suitable for being prepared into a biocontrol inoculant to replace a chemical agent to be used for preventing and controlling the tobacco bacterial wilt.

This invention relates to GM002714 The application of genes, recombinant vectors containing these genes, and recombinant bacteria in increasing the yield of Streptomyces natural products belongs to the field of genetic engineering technology. To discover and utilize yield-related genetic elements in the Streptomyces metabolic network to improve the yield of Streptomyces natural products, this invention utilizes transposon mutagenesis technology to discover and elucidate for the first time in Streptomyces the function of a two-component regulatory system involved in citric acid metabolism—TCS2713. By constructing a recombinant vector and recombinant bacteria containing the gene encoding the transcriptional regulator GM002714 of this two-component regulatory system, overexpression was observed in *Streptomyces avermitilis*, *Streptomyces cerevisiae*, *Streptomyces glabripennis*, or *Streptomyces roseospora*. GM002714 Genes can increase the yield of natural products. This invention provides valuable biosynthetic elements for the efficient biomanufacturing of Streptomyces.

This application relates to the field of pesticide production technology, specifically disclosing a fermentation production process for avermectin and avermectin itself. The avermectin fermentation production process involves inoculating *Streptomyces avermectin* into a culture medium for fermentation. The fermentation process is divided into five stages: 0-20 hours for the adaptation period, 21-80 hours for the growth period, 81-250 hours for the stationary period, and 251-300 hours for the aging period. The aeration rate during the adaptation period is 2000-2500 L / h, during the growth period it is 3000-3200 L / h, during the stationary period it is 2200-2500 L / h, and during the aging period it is 1900-2100 L / h. This avermectin fermentation process can achieve an avermectin yield of 6.89-7.11 g / L, effectively increasing avermectin production. Furthermore, the flexible adjustment of the aeration rate can effectively reduce gas waste, conserve resources, and improve overall economic efficiency.

The present application relates to a kind of extraction and purification method of abamectin, comprising the following steps: step 1, leaching: to the mycelium of Streptomyces avermitilis, organic solvent is added and leached, and the extract is collected;Step 2, impurity removal: after the extract is filtered, it is concentrated to 50% of the original extract volume after evaporation, the precipitate is removed by centrifugation, and the supernatant is obtained;Step 3, evaporation and concentration: the supernatant is continuously evaporated and concentrated, and the abamectin paste is obtained;Step 4, desugar: after the abamectin paste is desugared, the abamectin product after desugar is obtained;Step 5, crystallization: after adding crystallization solvent to the abamectin product after desugar, crystallization is carried out, solid-liquid separation is carried out, and the crystal is washed, and the abamectin finished product is obtained.The present application only needs to crystallize once to obtain the required abamectin finished product, reduces the crystallization times of abamectin, improves the production efficiency, and the yield of abamectin is improved, and the production cost is reduced.

The application discloses a micro-white Streptomyces avermitilis strain SaTC2601 and application thereof, and belongs to the field of biological control. The micro-white Streptomyces avermitilis strain SaTC2601 is isolated from the excrement of white star flower chafer (WSFC) larvae, and has a preservation number of CGMCC No.33741. The strain has a good prevention and treatment effect on peanut white thread blight caused by Sclerotium rolfsii on peanut roots, and also has a prevention and treatment effect on soil-borne pathogenic bacteria such as Fusarium graminearum, Fusarium solani, Fusarium moniliforme, Fusarium oxysporum, Rhizoctonia solani and Streptomyces scabiei. Further, the strain SaTC2601 also has a good growth promotion effect. The strain becomes a valuable resource for new drug development and agricultural disease prevention and control, and is expected to play a huge role in soil disease prevention and control.

The application discloses a solid culture medium for fermenting avermectin by using agricultural wastes and an application thereof, and belongs to the technical field of microbial fermentation. In order to solve problems of large water resource and organic reagent consumption, high energy consumption, high cost, environmental unfriendliness and the like in the production of avermectin by using liquid fermentation, and to solve the problem of low utilization rate of agricultural wastes, the optimal agricultural wastes are screened as the solid fermentation culture medium of Streptomyces avermitilis, and the green solid fermentation strategy of Streptomyces avermitilis is developed by optimizing the solid fermentation culture medium of Streptomyces avermitilis and fermentation process parameters, so as to reduce the fermentation cost, reduce the waste of equipment energy consumption, water resource and the generation of waste water and waste residue, and to realize the application of avermectin at a lower cost and in a green and environmental-friendly manner.

The invention discloses genetically engineered streptomyces as well as a construction method and application thereof. According to the present invention, with the application of the CRISPR-Cas9 gene editing technology, the aveA3 fragment in the Streptomyces avermitilis gene cluster capable of generating the ivermectin B1b compound is replaced with the milA3 fragment in the Streptomyces hygroscopicus gene cluster so as to obtain the gene engineering Streptomyces, such as Streptomyces avermitilis HU501-M, and the gene engineering Streptomyces HU501-M can be used to produce the ivermectin B1b compound, the constructed genetically engineered streptomyces can be used for preparing milbemycin D, and the compound has efficient insecticidal, acaricidal and antiparasitic activity, is high in safety to human beings and animals, and is environment-friendly. The main fermentation product of the genetically engineered streptomyces is milbemycins D, and the genetically engineered streptomyces has a good industrial development prospect.

The application discloses a streptomyces avermitilis-purpureococcus texanus compound microbial agent and a preparation device and method thereof, which comprises a strain preparation module, a culture medium configuration module, a compound fermentation module and a centrifugal purification module, wherein the compound fermentation module comprises a fermentation tank, a detection mechanism for detecting the compound microbial agent, a control mechanism for controlling the detection time interval and a cleaning mechanism; the detection mechanism comprises a detection rack arranged in the fermentation tank in a lifting mode, a plurality of detection barrels fixed on the detection rack, a protective barrel rotatably arranged outside the detection barrel, a pH sensor arranged in the detection barrel and a driving assembly for controlling the rotation of the protective barrel, the detection barrel is provided with a detection hole, the protective barrel is provided with a protective hole, and the inner wall of the protective barrel blocks the detection hole when the protective barrel is in an initial position; and the cleaning mechanism is used for cleaning the pH sensor. The compound microorganism in the application combines the respective advantages of the three kinds of microorganisms and can more widely prevent and control various pests, nematodes and pathogenic bacteria.