6 results about "Ethyl methanesulfonate" patented technology

An Aspergillus niger mutant strain designated MTCC 25759 derived from parent strain Aspergillus niger ATCC 9142 through chemical mutagenesis using ethyl methane sulfonate at 1 mg / mL for 30 to 60 minutes demonstrates enhanced production capabilities for both citric acid and gluconic acid. The mutant strain exhibits genetic modifications at 47 specific sequence positions that contribute to improved metabolic pathways, enhanced substrate utilization efficiency, and increased tolerance to acidic fermentation conditions. The mutant strain achieves gluconic acid production of 198.836 g / L representing a 66% improvement over the parent strain's 119.776 g / L yield, and citric acid production reaching 0.415 g / L with consistent late-phase productivity. The enhanced production capabilities provide economic advantages through improved substrate conversion rates, reduced raw material costs, and increased production capacity within existing fermentation infrastructure while maintaining the Generally Regarded As Safe (GRAS) status for commercial applications.

The present application relates to a kind of high-yield abamectin B2 Streptomyces avermitilis AVE-16 and its preparation method, the Streptomyces avermitilis AVE-16 is preserved in China Microbial Culture Collection Center, and the preservation number is CGMCC No.38200.The present application uses Streptomyces avermitilis XB-12 as starting strain, uses ethyl methanesulfonate and atmospheric pressure plasma combined mutagenesis technology to obtain a Streptomyces avermitilis AVE-16, and the yield of abamectin B2a is increased by 145% compared with starting strain, and the total yield of abamectin (B1a+B2a) is increased by 44% compared with starting strain.

This invention provides a method for mutagenesis of *Aspergillus cristatus* and its application, belonging to the field of microbial technology. The mutagenesis method provided by this invention includes the following steps: first, pretreating *Aspergillus cristatus* suspension by mixing it with a compound enzyme solution; then culturing the pretreated *Aspergillus cristatus* in a culture medium supplemented with a mutagen; the compound enzyme solution consists of a cell-lysing enzyme solution and a β-glucanase solution; the mutagen includes ethyl methanesulfonate and / or diethyl sulfate. This invention can improve the efficiency of the mutagen entering the bacterial cells, thereby enhancing the mutagenesis effect; the mutant strains obtained by mutagenesis, compared to the original strain, exhibit more vigorous colony growth on plates, larger average diameter, denser hyphae, and / or better morphological stability. Furthermore, it can achieve ideal mutagenic selection pressure at lower mutagen concentrations and shorter treatment times, reducing sublethal damage, which is beneficial for the recovery and screening of mutant strains and improving the efficiency of mutagenesis experiments.

This invention discloses a method and its application for creating stress-resistant mutants of common bean using chemical mutagenesis. The main steps include: determining the screening concentration of hydroxyproline (Hyp), determining the median lethal dose of ethyl methanesulfonate (EMS), and subculturing the stress-resistant mutants. After 3-5 subcultures, the stress-resistant mutants screened by this invention ultimately yielded 31 cotyledonary nodes of common bean resistant to hydroxyproline (Hyp), with a mutation rate of 8.16%. This invention can induce mutagenesis and directionally screen for stress-resistant common bean mutants, providing material support for stress-resistant breeding of common bean.

This invention relates to the field of crop breeding technology, and in particular to a method for inducing mutagenesis in wheat breeding. The invention involves sequentially soaking wheat seeds in a sodium azide solution, an N-nitroso-N-methylurea solution, and an ethyl methanesulfonate solution to obtain new wheat germplasm with phenotypic variations. This invention, by combining sodium azide, N-nitroso-N-methylurea, and ethyl methanesulfonate for mutagenesis, can obtain dwarf wheat plants and improve ear length and yield.

This invention provides a chemical mutagenesis screening method and its application for high-yield astaxanthin-producing Haematococcus pluvialis. The method involves chemically mutagenesizing Haematococcus pluvialis cells in the logarithmic growth phase with ethyl methanesulfonate, followed by single-colony screening to obtain high-yield astaxanthin mutants. After inducing astaxanthin accumulation under suitable culture conditions, the algae are freeze-dried, and then astaxanthin is extracted using a eutectic solvent system formed by thymol and oleic acid. This improves astaxanthin yield and extraction efficiency while reducing environmental pollution caused by traditional organic solvent extraction. This invention is simple to operate, has high extraction efficiency, and shows promising application prospects.