15 results about "Ethyl methanesulfonate" patented technology

This invention discloses a *Propionibacterium japonicum* strain selected through space-based combined chemical mutagenesis and its mutagenesis, screening, and propionic acid fermentation methods. Using *Propionibacterium japonicum* strain CICC22741 from the China Industrial Microbiological Culture Collection Center as the starting strain, this invention employs space-based mutagenesis via the "Shijian-19" satellite combined with ethyl methanesulfonate chemical mutagenesis. After two rounds of mutagenesis screening, a high-propionic acid-producing strain, *Propionibacterium japonicum* DJ04-16, was obtained. This strain was deposited at the China General Microbiological Culture Collection Center on June 16, 2025, with accession number CGMCC No. 34914. Subsequently, the propionic acid fermentation conditions of *Propionibacterium japonicum* DJ04-16 were optimized. Validation was performed in a 1-ton fermentation tank. After 96 hours of fermentation, the highest propionic acid yield reached 67.36 g / L, with a fermentation intensity of 0.702 g / L / h. This strain exhibits good industrial fermentation potential.

The invention provides bacillus methylotrophicus N7, a preparation and application of the bacillus methylotrophicus N7 to prevention and control of soybean cyst nematode, and belongs to the technical field of microorganisms. The preservation number of the bacillus methylotrophicus N7 is CGMCC (China General Microbiological Culture Collection Center) No.31391, and the bacillus methylotrophicus N7 is obtained by mutagenizing an original strain The strain is suitable for soybean low-temperature producing areas at 5-20 DEG C, has high soybean cyst nematode resistance, the cyst inhibition rate of a high-concentration group in the seedling stage is over 70%, the soybean yield in the mature stage is increased by 24.6% compared with a blank control, and the strain is non-toxic, free of residues and eco-friendly to soil.

The invention discloses a corn kernel linoleic acid content negative regulation gene ZmRPL31 as well as a mutant and application thereof, and a candidate gene ZmRPL31 closely related to linoleic acid anabolism in a high-linoleic acid corn selfing line YM-2 is successfully screened based on BSA (Bovine Serum Albumin) population construction and RNA-seq transcriptome analysis. Corn B73 pollen is treated through an ethyl methanesulfonate mutagenesis technology, and a rpl31 homozygous mutant strain is successfully constructed. GC-MS detection is carried out on the linoleic acid content of a homozygous mutant strain, and it is confirmed for the first time that the ribosomal protein gene ZmRPL31 has a negative regulation effect on synthesis of corn linoleic acid. Wherein the linoleic acid content of the rpl31-22 homozygous mutant strain in grain tissues is remarkably increased by 43.7% compared with that of wild type corn B73, and meanwhile, the linoleic acid content of the rpl31-22 homozygous mutant strain in leaves in the seedling stage is remarkably increased by 23.5% compared with that of the wild type corn B73.

An Aspergillus niger mutant strain designated MTCC 25759 derived from parent strain Aspergillus niger ATCC 9142 through chemical mutagenesis using ethyl methane sulfonate at 1 mg / mL for 30 to 60 minutes demonstrates enhanced production capabilities for both citric acid and gluconic acid. The mutant strain exhibits genetic modifications at 47 specific sequence positions that contribute to improved metabolic pathways, enhanced substrate utilization efficiency, and increased tolerance to acidic fermentation conditions. The mutant strain achieves gluconic acid production of 198.836 g / L representing a 66% improvement over the parent strain's 119.776 g / L yield, and citric acid production reaching 0.415 g / L with consistent late-phase productivity. The enhanced production capabilities provide economic advantages through improved substrate conversion rates, reduced raw material costs, and increased production capacity within existing fermentation infrastructure while maintaining the Generally Regarded As Safe (GRAS) status for commercial applications.

The present application relates to a kind of high-yield abamectin B2 Streptomyces avermitilis AVE-16 and its preparation method, the Streptomyces avermitilis AVE-16 is preserved in China Microbial Culture Collection Center, and the preservation number is CGMCC No.38200.The present application uses Streptomyces avermitilis XB-12 as starting strain, uses ethyl methanesulfonate and atmospheric pressure plasma combined mutagenesis technology to obtain a Streptomyces avermitilis AVE-16, and the yield of abamectin B2a is increased by 145% compared with starting strain, and the total yield of abamectin (B1a+B2a) is increased by 44% compared with starting strain.

This invention provides a method for mutagenesis of *Aspergillus cristatus* and its application, belonging to the field of microbial technology. The mutagenesis method provided by this invention includes the following steps: first, pretreating *Aspergillus cristatus* suspension by mixing it with a compound enzyme solution; then culturing the pretreated *Aspergillus cristatus* in a culture medium supplemented with a mutagen; the compound enzyme solution consists of a cell-lysing enzyme solution and a β-glucanase solution; the mutagen includes ethyl methanesulfonate and / or diethyl sulfate. This invention can improve the efficiency of the mutagen entering the bacterial cells, thereby enhancing the mutagenesis effect; the mutant strains obtained by mutagenesis, compared to the original strain, exhibit more vigorous colony growth on plates, larger average diameter, denser hyphae, and / or better morphological stability. Furthermore, it can achieve ideal mutagenic selection pressure at lower mutagen concentrations and shorter treatment times, reducing sublethal damage, which is beneficial for the recovery and screening of mutant strains and improving the efficiency of mutagenesis experiments.

This invention discloses a method and its application for creating stress-resistant mutants of common bean using chemical mutagenesis. The main steps include: determining the screening concentration of hydroxyproline (Hyp), determining the median lethal dose of ethyl methanesulfonate (EMS), and subculturing the stress-resistant mutants. After 3-5 subcultures, the stress-resistant mutants screened by this invention ultimately yielded 31 cotyledonary nodes of common bean resistant to hydroxyproline (Hyp), with a mutation rate of 8.16%. This invention can induce mutagenesis and directionally screen for stress-resistant common bean mutants, providing material support for stress-resistant breeding of common bean.

The invention discloses a mutagenesis method and a cultivation method of industrial cannabis sativa, the mutagenesis method comprises the following steps: (1) soaking industrial cannabis sativa seeds with ethyl methanesulfonate, the concentration of the ethyl methanesulfonate being 0.05-0.4 mol / L, and the soaking time being 15-60 min; and (2) planting the mutagenized industrial cannabis sativa seeds in a greenhouse, eliminating plants with low cannabis sativa terpene phenol content generation by generation through continuous multi-generation selection, carrying out genetic diversity and genetic relationship analysis by utilizing a molecular marker technology, and screening the industrial cannabis sativa with improved cannabis sativa terpene phenol content. According to the method, ethyl methanesulfonate with specific concentration is matched with specific soaking time, the mutagenesis rate can be increased under optimized mutagenesis conditions, screening of variant plants is facilitated, and screening of industrial cannabis sativa with increased cannabinoid phenol content is facilitated.

The application provides a methylotrophic bacillus N7, a preparation and application thereof in prevention and control of soybean cyst nematode, and belongs to the technical field of microorganisms. The methylotrophic bacillus N7 has a preservation number of CGMCC No.31391, and is obtained by mutagenizing an original strain N1 with 0.4% ethyl methanesulfonate. The methylotrophic bacillus N7 is suitable for a low-temperature soybean production area with a temperature of 5 to 20 DEG C, has high resistance to soybean cyst nematode, a cyst inhibition rate of a high-concentration group in a seedling stage is higher than 70%, a soybean yield in a mature stage is increased by 24.6% compared with a blank control, is non-toxic and non-residual, and is friendly to soil ecology.

This invention relates to the field of crop breeding technology, and in particular to a method for inducing mutagenesis in wheat breeding. The invention involves sequentially soaking wheat seeds in a sodium azide solution, an N-nitroso-N-methylurea solution, and an ethyl methanesulfonate solution to obtain new wheat germplasm with phenotypic variations. This invention, by combining sodium azide, N-nitroso-N-methylurea, and ethyl methanesulfonate for mutagenesis, can obtain dwarf wheat plants and improve ear length and yield.

This invention provides a chemical mutagenesis screening method and its application for high-yield astaxanthin-producing Haematococcus pluvialis. The method involves chemically mutagenesizing Haematococcus pluvialis cells in the logarithmic growth phase with ethyl methanesulfonate, followed by single-colony screening to obtain high-yield astaxanthin mutants. After inducing astaxanthin accumulation under suitable culture conditions, the algae are freeze-dried, and then astaxanthin is extracted using a eutectic solvent system formed by thymol and oleic acid. This improves astaxanthin yield and extraction efficiency while reducing environmental pollution caused by traditional organic solvent extraction. This invention is simple to operate, has high extraction efficiency, and shows promising application prospects.

The invention relates to a method for cultivating a saline-alkaline tolerant asparagus material by combining compound mutagenesis with high-salt screening, and belongs to the technical field of saline-alkaline tolerant crop cultivation, and the alternate circulating compound mutagenesis treatment method comprises the following steps: carrying out alternate circulating compound mutagenesis treatment on the asparagus material by adopting pingyangmycin and ethyl methanesulfonate; the multi-generation dynamic high-salt screening method comprises the following steps: carrying out multi-generation dynamic high-salt screening on the asparagus materials subjected to alternate cyclic compound mutagenesis treatment; the recovery culture method comprises the step of carrying out recovery culture on the screened asparagus materials. The asparagus with genetic stability and strong salt tolerance is directionally and quickly bred by combining efficient compound mutation with multi-generation dynamic stress screening for simulating a real environment; stress is applied in the seedling stage, extremely sensitive individuals can be rapidly eliminated, the culture cost and space are saved, it is ensured that only the individuals with the strongest salt tolerance can survive and breed, and salt-tolerant genes are enriched generation by generation.

The present invention relates to a method for purifying a crude product of ethoxyfen such that it does not contain one or more impurities selected from the group consisting of ethyl methanesulfonate, isobutyl methanesulfonate, and any combination thereof. The process involves converting the impurities into by-products that are miscible with water. The invention further relates to a suspension concentrate formulation comprising ethofuroxol.

The invention relates to the technical field of agricultural biology, in particular to a rice mutant protein, a mutant gene and application of the rice mutant protein and the mutant gene. The amino acid sequence of the rice mutant protein provided by the invention is as shown in SEQ ID NO. 1. The method comprises the following steps: carrying out large-scale mutagenesis on calluses of Nipponbare rice by using ethyl methanesulfonate, adding a herbicide for screening during culture, carrying out differentiation, rooting and planting on the obtained resistant calluses, selecting a rice variety with high thousand seed weight, and sequencing to obtain the rice mutant protein. According to the rice mutant protein, the thousand seed weight of rice can be remarkably increased, the grain length of seeds is increased, the yield of the rice is increased, and the rice can have the weeding resistance function.

The invention discloses propionibacterium jensenii bred by aerospace superposition chemical mutation and a method for mutagenizing, screening and fermenting propionic acid by the propionibacterium jensenii. The propionibacterium jensenii CICC22741 with high propionic acid yield is obtained by taking the propionibacterium jensenii CICC22741 as an original strain and through superposition of'practice No.19 'satellite space mutagenesis and ethyl methanesulfonate chemical mutagenesis and through two rounds of mutagenesis screening, the propionibacterium jensenii DJ04-16 with high propionic acid yield is obtained. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on June 16, 2025, and the preservation number is CGMCC No.34914. The invention further discloses a preparation method of the strain. Then, the propionic acid fermentation condition of the propionibacterium jensenii DJ04-16 is optimized, fermentation in a 1-ton tank verifies that the maximum propionic acid yield reaches 67.36 g / L and the fermentation intensity is 0.702 g / L.h after fermentation is performed for 96 h, and the strain has good industrial fermentation potential.