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37 results about "Ethyl methanesulfonate" patented technology
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Ethyl methanesulfonate (EMS) is a mutagenic, teratogenic, and possibly carcinogenic organic compound with formula C₃H₈SO₃. It produces random mutations in genetic material by nucleotide substitution; particularly by guanine alkylation. This typically produces only point mutations. It can induce mutations at a rate of 5x10⁻⁴ to 5x10⁻² per gene without substantial killing. The ethyl group of EMS reacts with guanine in DNA, forming the abnormal base O⁶-ethylguanine. During DNA replication, DNA polymerases that catalyze the process frequently place thymine, instead of cytosine, opposite O⁶-ethylguanine. Following subsequent rounds of replication, the original G:C base pair can become an A:T pair (a transition mutation). This changes the genetic information, is often harmful to cells, and can result in disease.
Piperazine salt and a process for the preparation thereof
ActiveUS20110275816A1High purityHigh yieldOrganic chemistryBulk chemical productionEthyl methanesulfonate2,3-Dichlorophenylpiperazine
The invention relates to novel trans N-{4-{2-[4-(2, 3-dichlorophenyl)-piperazine-1-il]-ethyl}-cyclohexylamine dihydrochloride monohydrate and a process for the preparation of the trans N-{4-{2-[4-(2,3-dichlorophenyl)-piperazine-1-il]-ethyl}-cyclohexylamine dihydrochloride monohydrate, said process comprising the stepsa) reacting trans 2-{1-[4-(N-tert-butoxycarbonyl) amino]-cyclohexyl}-acetic acid ester with sodium borohydride and aluminium trichloride to give trans 2-{1-[4-(N-tert-butoxycarbonyl)-amino]-cyclohexyl}-ethanol;b) reacting trans 2-{1-[4-(N-tert-butoxycarbonyl)-amino]cyclohexyl}-ethanol obtained with methanesulfonic acid chloride in the presence of an acid binding agent to give trans 2-{1-[4-(N-tert-butoxycarbonyl)-amino]-cyclohexyl}-ethyl methanesulfonate;c) reacting trans 2-{1-[4-(N-tert-butoxycarbonyl)-amino]-cyclohexyl}-ethyl methanesulfonate obtained with 2,3-dichlorophenyl-piperazine in the presence of an acid binding agent to give trans 2-{1-[4-(N-tert-butoxycarbonyl)-amino]-cyclohexyl}-carbamic acid tert-butylester;d) heating trans 2-{1-[4-(N-tert-butoxycarbonyl)-amino]-cyclohexyl}-carbamic acid tert-butylester obtained to a temperature between 40-100° C. in a mixture of aqueous hydrochloric acid / methanol to give trans N-{4-{2-[4-(2,3-dichlorophenyl) piperazine-1-il]-ethyl}-cyclohexylamine dihydrochloride monohydrate.
Owner:RICHTER GEDEON NYRT
Method for cultivating tobacco mutants on basis of EMS (ethyl methanesulfonate) mutagenesis
InactiveCN107667855ASeed and root treatmentPlant genotype modificationNicotiana tabacumMethyl methanesulfonate
The invention belongs to the technical field of tobacco breeding and particularly relates to a large-scale efficient cultivation method for tobacco mutants on the basis of an EMS (ethyl methanesulfonate) mutagen. The method comprises steps of pretreatment of tobacco seeds, treatment with EMS, seed germination and transplanting, screening of variant plants as well as calculation of germination rateand survival rate. EMS treatment conditions are particularly optimized for main tobacco cultivar K326, the tobacco K326 is enabled to be sub-vital through probing, and the optimal EMS treatment conditions are obtained. The method can be applied to construction of a tobacco mutant library, can be directly applied to breeding of new varieties and has the advantage that advanced backcross is not needed, and genetic breeding resources of tobacco are enriched.
Owner:YUNNAN ACAD OF TOBACCO AGRI SCI
Breeding method for tetraploid maize
InactiveCN105409764AHigh mutagenic powerNot rotten seedlingsPlant genotype modificationBiotechnologyNitroso
The invention discloses a breeding method for tetraploid maize. The breeding method comprises the steps of seed selection, seed germination culture, mutagenesis and seedling culture, wherein the mutagenesis step adopts chemical mutagenesis; the mutagenesis method of a maize tetraploid is high in mutagenesis success rate, and free of seedling rotting. A mutagen adopted in chemical mutagenesis comprises a mixture of colchicine, heteroauxin, periethylenenaphthalene, ethyl methanesulfonate, ethylenimine, nitroso-ethyl-urethane, nitroso-methyl-urethane, diethyl sulphate, azaserine, and mitomycin C. The mutagenesis method of the maize tetraploid is high in mutagenesis success rate, and free of seedling rotting, and has a wide market prospect; the chemical mutagen adopted in the mutagenesis method is obtained via precise proportioning, and can realize accurate induction of tetraploid maize; the treated maize is high in yield, short in growth cycle, and has competitive advantage; the breeding method is simple to operate, and easy for wide-range popularization.
Owner:HUIAN KELIAN AGRI TECH CO LTD
Short-day sensitive cell death SSD1 gene and coded protein and application thereof
The invention discloses a short-day sensitive cell death SSD1 gene and a coded protein and application thereof. The SSD1 gene has a sequence as shown in SEQ ID NO:1. According to the invention, by using a model plant arabidopsis thaliana as a test material, through EMS (ethyl methanesulfonate) mutagenesis, an ssd1 mutant is obtained through screening. The SSD1 gene is separated by adopting a map-based cloning method, a gene mutational site is found, and the cell death is caused by deletion of the gene under the condition of short day. The SSD1 gene is necessary for the growth of arabidopsis thaliana under the condition of short day, and has a wide homology in plants. The finding of the biological function of the gene and the relevant research on a homologous gene are beneficial to deepening of the research on the controlling of the plant cell death by photoperiod, have important scientific significance in researching a molecular mechanism of the plant cell death, and also have important application value in improvement of crop varieties in a short-day region.
Owner:HUNAN AGRICULTURAL UNIV
Synthesis of chiral 2,2'-bi(diphenyl phosphine)-1,1'-binaphthalene
InactiveCN1982318AHigh yieldSimple purification methodGroup 5/15 element organic compoundsDiphenyl phosphateHalogen
Synthesis of chiral 2,2'-bi(diphenyl phosphine)-1,1'-binaphthalene is carried out by reacting 2,2'-bi(ethyl methanesulfonate trifluoroacetate)-1,1'-binaphthalene with diphenyl phosphate halogen under action of complex catalyst and auxiliaries to generate the final product. It's simple, has more yield and less by-products.
Owner:AOPUDISHI CHEM SCI & TECH SHANGHAI
Novel automobile accessory spraying technology
InactiveCN108686902ASimple preparation processSpray the surface evenlyPretreated surfacesCoatingsZincSilicon dioxide
The invention provides a novel automobile accessory spraying technology. Cleaning, grinding and static electricity removal treatment is conducted on the surface of an automobile accessory; a coating material is prepared, ethyl methanesulfonate, 2-methacrylamide, nano-molybdenum disulfide, teflon, nano silicon dioxide, zinc powder, magnesium stearate and ethidene diamine are mixed, the temperatureis increased to 100-120 DEG C, and stirring is conducted for 25-35 min; then, under the power of 25-35 KW, ultrasonic treatment is conducted for 30-45 min; a medium-caliber spray gun is adopted to spray the coating material two times, the first-time spraying pressure is 0.6-0.7 MPa, the spraying distance is 15-25 cm, and the angle between the sprayed face and an automobile accessory plane is 65-75degrees; the second-time spraying pressure is 1.0-1.25 MPa, the spraying distance is 30-50 cm, the angle between the sprayed face and the automobile accessory plane is 80-90 degrees, and the intervaltime between two times of spraying is 8-12 min; and the sprayed automobile accessory can be obtained after grinding, drying and polishing treatment.
Owner:苏州耐思特塑胶有限公司
Cesium-137 mutagenesis breeding method for macadimia nuts
ActiveCN112602591ARaise the ratioOptimal treatment processPlant genotype modificationBiotechnologyMacadamia spp
The invention relates to a cesium-137 mutagenesis breeding method for macadimia nuts. Macadimia nut seeds are subjected to sterilization treatment firstly, so that disease bacteria on the upper surfaces of the macadimia nut seeds can be killed, disease propagation is reduced, and field diseases are prevented. The seeds obtained after sterilization treatment are put into pretreatment liquid to be soaked, so that the effect of strengthening the chemical mutagenesis effect can be achieved, and chemical mutagenesis of the macadimia nut seeds is promoted. A chemical mutagenic agent is a mixture of ethyl methanesulfonate and diethyl sulfate, so that the mutation speed of the macadimia nut seeds can be increased, the mutation rate can be increased, and seeds with special characters can be obtained. A chemical mutagenesis treatment process is also limited and is divided into two times of mutagenesis treatment, so that the mutagenesis effect is better. Chemical mutagenesis treatment and 137Cs-gamma ray irradiation treatment are combined, so that the mutation speed of the macadimia nut seeds is further increased, the mutation rate is increased, and the seeds with special characters can be obtained. The seeds obtained after irradiation treatment are subjected to oscillation treatment, so that the mutation speed of the macadimia nut seeds can be further increased, and the mutation rate is increased.
Owner:SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI
Method for treating amaranth seeds by adopting ethyl methanesulfonate
PendingCN108849491ASimplify the Mutation Breeding ProcessThe breeding operation process is simple and effectivePlant genotype modificationMethyl methanesulfonateEthyl ester
The invention belongs to the field of crop seed breeding and in particular relates to a method for treating amaranth seeds by adopting ethyl methanesulfonate. According to the method provided by the invention, the amaranth seeds are selected as a starting receptor material for mutagenic treatment, and the amaranth seeds are subjected to the mutagenic treatment by utilizing an ethyl methanesulfonate solution with the concentration of 0 to 0.8 v / v percent; the amaranth seeds subjected to the mutagenic treatment are transferred into a culture dish paved with three layers of wet filter paper, andare continually cultured for several days under the conditions that the temperature is 20 to 25 DEG C, the illumination time is 12h / d and the intensity is 1500 to 2000lx; then the sprouted amaranth seeds are transferred into a hole disc with 72 holes and are continually cultured until the seeds grow to an M0 generation, and mutants are screened from the seeds. By adopting the method provided by the invention, the limitation of an existing seed breeding technology is overcome, and the mutagenic effect of the methanesulfonate on the amaranth seeds is improved; the method plays an active role onimprovement of the seed breeding efficiency of amaranths and promotion of sustainable development of an amaranth industry.
Owner:NANJING AGRICULTURAL UNIVERSITY
Short-day sensitive cell death SSD1 gene and coded protein and application thereof
The invention discloses a short-day sensitive cell death SSD1 gene and a coded protein and application thereof. The SSD1 gene has a sequence as shown in SEQ ID NO:1. According to the invention, by using a model plant arabidopsis thaliana as a test material, through EMS (ethyl methanesulfonate) mutagenesis, an ssd1 mutant is obtained through screening. The SSD1 gene is separated by adopting a map-based cloning method, a gene mutational site is found, and the cell death is caused by deletion of the gene under the condition of short day. The SSD1 gene is necessary for the growth of arabidopsis thaliana under the condition of short day, and has a wide homology in plants. The finding of the biological function of the gene and the relevant research on a homologous gene are beneficial to deepening of the research on the controlling of the plant cell death by photoperiod, have important scientific significance in researching a molecular mechanism of the plant cell death, and also have important application value in improvement of crop varieties in a short-day region.
Owner:HUNAN AGRICULTURAL UNIV
Mutagenesis method of seed lotus phenotypic variation mutant plant
Owner:WUHAN ACADEMY OF AGRI SCI
Cultivation method for novel variety by inducing mutation of tender stem tissues of epimedium koreanum
ActiveCN111316912ANot deformedDominant mutation highPlant tissue cultureHorticulture methodsBiotechnologyEpimedium koreanum
The invention belongs to the technical field of plant propagation, and specifically relates to a cultivation method for a novel variety by inducing mutation of tender stem tissues of epimedium koreanum. According to the cultivation method for the novel variety by inducing mutation of the tender stem tissues of the epimedium koreanum provided by the invention, with tender stems of the epimedium koreanum materials, by combination of ethyl methanesulfonate (EMS) with a plant tissue culture technology and adoption of methods of embryonic callus induction, redifferentiation, embryoid rejuvenation and the like, an artificially cultivated novel variety of the epimedium koreanum, which is strong in independence of each single plant, high in genetic information purity of each single plant and clearin single plant source, is obtained; and the cultivation method has important protection and ecological significance for amplification of an epimedium koreanum population; meanwhile, the cultivationmethod can be further applied to construction of an epimedium koreanum mutant library; and the novel variety cultivated by using the cultivation method provided by the invention can be directly used as a strain for cultivation and variety certification.
Owner:TONGHUA NORMAL UNIV +1
Method for detecting methanesulfonate genotoxic impurities in gemcitabine hydrochloride by GC-MS/MS method
ActiveCN111707747AReduce stepsThe extraction method is convenient and simpleComponent separationGas liquid chromatographicChromatography column
Owner:江西省药品检验检测研究院
Construction method of laver mutant library
PendingCN113349052AHigh mutation rateHigh mutation saturationAlgae productsCultivating equipmentsBiotechnologyGenetics
The invention discloses a construction method of a laver mutant library, and belongs to the technical field of plant breeding. According to the construction method of the laver mutant library, the mutant library with high mutation saturation is obtained by taking porphyra yezoensis monospores as a mutagenic material and ethyl methanesulfonate as a mutagenic agent and optimizing mutagenic conditions, so that the mutant library has mutants with various characters, the obtained mutagenic progeny is a pure line and is relatively high in mutation rate, and abundant germplasm resources are provided for germplasm breeding and genetic research of the laver.
Owner:OCEAN UNIV OF CHINA
A highly efficient mutagenesis method for tobacco pollen
InactiveCN102293154AIncreased sensitivityEasy maintenancePlant tissue cultureHorticulture methodsNicotiana tabacumLiquid medium
The invention relates to a high-efficiency mutagenesis method of nicotiana tabacum L. pollen. The high-efficiency mutagenesis method comprises the following steps of putting nicotiana tabacum L. pollen into a liquid medium under an aseptic condition to carry out mutagenesis treatment by 0.1 to 0.2% of ethyl methanesulfonate (EMS) in a dark place at a temperature of 30 to 32 DEG C, carrying out chromosome doubling treatment by a NLN-16 liquid medium in a dark environment at a temperature of 30 to 32 DEG C, putting the nicotiana tabacum L. pollen obtained by the previous step into a NLN-13 liquid medium to carry out embryo induction culture in a dark environment at a temperature of 24 to 26 DEG C, carrying out shake culture by a shaking table in a dark environment at a temperature of 24 to 26 DEG C when an immature embryo appears, and putting the immature embryo growing a cotyledon into a solid medium to carry out subculture, wherein during a transplanting season, after seedling hardening, a seedling obtained by the high-efficiency mutagenesis method can be transplanted in a field for mutant character identification. The high-efficiency mutagenesis method overcomes dominant and recessive barriers of gene expression, is beneficial for selection, shortens greatly a mutant character stabilization period, saves breeding generation time, and has the characteristics of wide variation range, high mutagenesis frequency and the like.
Owner:TOBACCO RES INST OF HUBEI PROVINCE
Preparation method of silodosin intermediate
ActiveCN109516933AHigh purityNew routeOrganic compound preparationSulfonic acid esters preparationChemical synthesisBenzaldehyde
The invention discloses a preparation method of a silodosin intermediate and relates to the technical field of chemical synthesis of drugs. The preparation method comprises the following steps: subjecting salicyaldehyde and ethylene carbonate to transesterification to obtain 2-(2-hydroxyethoxy)benzaldehyde; then, carrying out a Dakin oxidation reaction to obtain sodium 2-(2-hydroxyethoxy) phenol;then, subjecting sodium 2-(2-hydroxyethoxy) phenol and trifluoroethanol to an etherification reaction to obtain 2-[2-(2,2,2-trifluoroethyoxy)phenoxy]ethyl alcohol; and finally, subjecting 2-[2-(2,2,2-trifluoroethyoxy)phenoxy]ethyl alcohol and methanesulfonyl chloride to an esterfication reaction to obtain the silodosin intermediate 2-[2-(2,2,2-trifluoroethyoxy)phenoxy]ethyl methanesulfonate. The preparation method is novel and short in synthesis route, and a target product can be prepared by only four-step reaction. Both raw materials and reagents used for preparation are cheap, available andenvironment-friendly, all the reaction conditions are mild and controllable, the preparation method is convenient and simple in operation, and the prepared silodosin intermediate is high in purity andyield, suitable for industrial production and wide in prospect and industrial application value.
Owner:ANHUI QINGYUN PHARMA & CHEM
Method for culturing sorghum seeds induced by ethyl methanesulfonate
ActiveCN110393148AImprove germination rateAccurate identificationCultivating equipmentsSoilless cultivationCulture mediumsEthyl fumarate
The invention provides a method for culturing sorghum seeds induced by ethyl methanesulfonate. The method comprises the following steps of (1) preparation of a seedling culture vessel; (2) preparationof a seedling culture medium, wherein the seedling culture medium is prepared, disinfected and air-dried; (3) preparation of a seedling culture site, wherein a layer of sand with the thickness of 1-2cm is laid on the upper portion of the ground of a sunlight glass greenhouse, and the lower layer is soil; (4) sowing; (5) seedling culture management; (6) transplanting, wherein seedlings are transplanted into a field when the seedling age is 35-50 days and the seedling height is 5 cm or above. According to the method, by carrying out greenhouse seedling culture on the seeds obtained after EMS mutagenesis, the emergence rate of mutagenetic offspring can be obviously increased, the maturity of mutagenetic single plants is accelerated, a nearly theoretical number of mutagenetic offspring materials are obtained, and accurate identification of the mutation characters of M1-generation mutagenetic plants is facilitated.
Owner:甘肃省农业科学院生物技术研究所
Rape mutation breeding method
PendingCN114680041AIncreased resistance to SclerotiniaEarly maturityHorticulture methodsPlant tissue cultureBiotechnologyMutation breeding
The invention discloses an oilseed rape mutation breeding method, and relates to the technical field of oilseed rape breeding, a test-tube plantlet cultured by a cabbage type oilseed rape young blank is adopted for mutagenesis, firstly, MS is used as a culture medium and is added into a mutagenesis culture medium containing 0.3% of ethyl methanesulfonate for mutagenesis culture, then C5H9NO2 and C5H9NO3 are used for two rounds of screening, and the mutagenesis of the oilseed rape is realized. According to the present invention, the enzyme activity of the HMP pathway of the mutant screened by C5H9NO2 and C5H9NO3 is strong, and the determination result of the glucose-6-phosphate dehydrogenase activity in the rapeseed forming process proves that the synthesis of fatty acid is easily achieved, such that the yield and the oil content quality of the rape are improved, the sclerotiniose resistance is enhanced, and the mature period is advanced.
Owner:ANHUI JINPEIYIN TECH
Method for obtaining fusarium oxysporum resistant carnation clone
PendingCN112544442AEasy to operateShort cycleHorticulture methodsPlant tissue cultureBiotechnologyFusarium oxysporum
The invention relates to a method for obtaining a fusarium oxysporum resistant carnation clone, belongs to the technical field of plant tissue culture, and aims at obtaining a fusarium oxysporum resistant breeding intermediate material by inducing calluses by utilizing carnation, mutagenizing by adopting ethyl methanesulfonate (EMS) and adding fusarium oxysporum toxin screening. According to the method, a large number of cells can be cultured and treated in a smaller space by utilizing a technology of adding pathogenic toxin into a culture medium to screen disease-resistant germplasm after clone mutation of the cells; direct induction and screening of mutants at the cellular level is an attempt of higher plant resistance breeding microbiotization; and the disease-resistant breeding periodcan be shortened, directional breeding is conducted, the selection efficiency is high, the effect is good, the field breeding period is shortened, and a new method is provided for disease-resistant breeding of carnation.
Owner:FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
Breeding method for cellulase high-yield bacteria
InactiveCN110079518AMaintain enzyme production capacitySimple stepsMutant preparationMicroorganism based processesBiotechnologyTrichoderma reesei
The invention discloses a breeding method for cellulase high-yield bacteria. The breeding method comprises the following steps: 1) culturing trichoderma viride and trichoderma reesei in a first medium, followed by 60 Co-gamma ray irradiation to obtain a mutant mixed mold; 2) culturing clostridium thermocellum in a second medium, followed by ultraviolet irradiation to obtain a mutant strain; 3) culturing the mutant mixed mold and the mutant strain in an MS medium to obtain a cellulase-producing strain, wherein the first medium contains nitrosoguanidine and diethyl sulfate; and the second mediumcontains ethyl methanesulfonate and diethyl sulfate. The cellulase high-yield bacteria selected by the method are resistant to degradation.
Owner:ANHUI WUYUE FOOD
A seedling raising method for mutagenizing sorghum seeds with ethyl methanesulfonate
ActiveCN110393148BImprove germination rateAccurate identificationCultivating equipmentsSoilless cultivationBiotechnologyGreenhouse
The invention provides a method for raising seedlings of sorghum seeds induced by ethyl methanesulfonate, which comprises the following steps: (1) preparation of seedling raising containers; (2) preparation of seedling raising substrates: preparing seedling raising substrates and sterilizing and drying them; (3) Preparation of nursery site: choose to place in a solar glass greenhouse, spread a layer of 1‑2cm thick sand on the upper part of the ground, and the lower layer is soil; (4) sowing; (5) seedling management; (6) transplanting: seedling age 35‑ After 50 days, when the seedling height reaches more than 5 cm, the seedlings are transplanted to the field. In the present invention, by growing seedlings in a greenhouse for seeds induced by EMS, the emergence rate of the mutant offspring can be significantly improved, the early maturation of the mutant single plant can be promoted, and the number of mutant offspring materials close to the theoretical value can be obtained, which is beneficial to the M1 generation Accurate identification of mutant traits of mutagenic strains.
Owner:甘肃省农业科学院生物技术研究所
Novel variety cultivation method for inducing petal mutation of physalis alkekengi l
ActiveCN111296294ANot deformedDominant mutation highPlant tissue cultureHorticulture methodsBiotechnologyPetal
The invention belongs to the technical field of plant propagation, in particular to a novel variety cultivation method for inducing petal mutation of physalis alkekengi l. According to the novel variety cultivation method for inducing petal mutation of physalis alkekengi l, the cultivation method comprises the following steps: sterile petals are obtained through flower bud development and cultivation, pale green embryogenic calli are obtained by inducing embryogenic calli from the flower petals, the embryonic calli are induced by ethyl methanesulfonate to differentiate into embryoids with differences, the embryoids grow into individual plants and the like, so that a novel physalis alkekengi l cultivated variety with characteristics of strong independence, high purity of genetic informationand clear source and the like of the individual plants is obtained. The novel physalis alkekengi l cultivated variety is genetically stable, and can be utilized for strain cultivation and construction of physalis alkekengi l mutant library.
Owner:通化天妍生物技术有限公司 +1
Preparation method of high-content sisomicin fermentation liquor
InactiveCN105603026AHigh purification yieldHigh biological potencyMicroorganism based processesFermentationMicrowaveBiological value
The invention discloses a preparation method of high-content sisomicin fermentation liquor, which belongs to the field of the medicine synthesis, and aims at solving the problems of the existing sisomicin fermentation liquor that the biological value is relatively low, the difference between a maximum value and a minimum value is great, the production is unstable, the content of a main component of the sisomicin is not high and the extraction and purification yield is low. The method comprises the steps: performing the activation by virtue of micromonospora inyoensis, then performing induced variation for an activated bacterial strain under the condition of ethyl methanesulfonate and microwaves, placing the mutated bacterial strain in a fermentation tank, promoting generation of sisomicin in a cobalt chloride solution, generating the sisomicin under the initiation of a specific bacterial liquid, and then filtering and separating to obtain the high-content sisomicin fermentation liquor. The purification yield is high.
Owner:CHANGZHOU LANXU CHEM CO LTD
Method for producing pectinase preparation through solid-state fermentation of penicillium
PendingCN113502282AIncrease production capacitySimple and fast operationFungiMicroorganism based processesBiotechnologyPectinase
The invention discloses a method for producing a pectinase preparation through solid-state fermentation of penicillium, which comprises the following steps: screening a pectinase high-yield strain Penicilliumsp.Y through high-throughput screening, and optimizing solid-state fermentation conditions of the pectinase high-yield strain Penicilliumsp.Y, so that the activity of the finally produced pectinase preparation is as high as 13800U / g; according to the method for producing the pectinase preparation through solid-state fermentation of the penicillium, an enzyme-producing strain with the enzyme activity as high as 19000U / g is successfully screened out under the condition that the fatality rate is 85.8% by adopting an ethyl methanesulfonate (EMS) mutant strain, and the optimal culture medium components and growth conditions of the strain are determined through a subsequent optimization test: 20g of soybean meal, 68% of initial water content, 3% of glucose content, the content of peptone as 1%, the content of CaCl2 as 0.04%, the content of FeCl3 as 0.04%, the inoculum size as 4%, and the culture temperature as 38 DEG C; and the enzyme activity is up to 2200U / g after the strain is cultured for 5 days, so that the enzyme production capacity of the strain is greatly improved, and an ideal target is achieved.
Owner:江苏悠恒生物技术有限公司
A cesium-137 mutation breeding method for macadamia nuts
ActiveCN112602591BRaise the ratioOptimal treatment processPlant genotype modificationBiotechnologyMutation breeding
The invention discloses a cesium-137 mutation breeding method for macadamia nuts. The macadamia nut seeds are sterilized first, which can kill the disease bacteria on the upper surface of the macadamia nut seeds, reduce the spread of diseases, and prevent the occurrence of field diseases; put the sterilized seeds into Soaking treatment in the pre-treatment solution can strengthen the effect of chemical mutagenesis and promote the chemical mutagenesis of macadamia nut seeds; the chemical mutagen is a mixture of ethyl methanesulfonate and diethyl sulfate, which can accelerate the mutagenesis of macadamia nuts. Seed mutation speed, increase the mutation rate, and can obtain seeds with special traits; also limit the treatment process of chemical mutagenesis, which is divided into two mutagenesis treatments, so that the effect of mutagenesis is better; adopt chemical mutagenesis treatment and 137 The combination of Cs-γ-ray irradiation treatment can further accelerate the mutation speed of macadamia nut seeds, increase the mutation rate, and can obtain seeds with special traits; the irradiated seeds are subjected to shock treatment, which can further accelerate the mutation speed of macadamia nut seeds and improve mutation rate.
Owner:SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI
Rapid detection method of three commonly used anesthetics in aquatic products
ActiveCN113092604BSimple and fast operationEasy to operateComponent separationBenzoic acidIsoeugenol
The invention discloses a rapid detection method for three commonly used anesthetics in aquatic products, belonging to the technical field of inspection. The present invention utilizes a single quadrupole gas chromatography spectrometer (GC-MS) for screening, quantification and confirmation, adopts a Thermo TG 1701MS capillary column as an analysis column, and establishes and measures eugenol in aquatic products under a positive ion-selected ion monitoring mode (SIM) (Eugenol, CAS: 97‑53‑0), Isoeugenol (Isoeugenol, CAS: 97‑57‑1), ethyl 3‑aminobenzoate methanesulfonate (MS‑222, CAS: 886‑86‑ 2) Three methods of anesthetic content. The detection limits of the method were 25.0 μg / kg for eugenol and isoeugenol, and 50.0 μg / kg for ethyl 3-aminobenzoate methanesulfonate. The detection method is simpler and quicker than the traditional instrumental analysis method, and has the characteristics of good linear correlation, high sensitivity, resistance to matrix interference, high accuracy and high stability.
Owner:FISHERIES RES INST OF FUJIAN
Method for culturing new varieties for inducing mutation of rhizome slice tissues of Aralia elata(Miq)Seem.
ActiveCN111316913ANot deformedDominant mutation highSaving energy measuresPlant tissue cultureBiotechnologyAralia elata
The invention belongs to the technical field of plant propagation, in particular to a method for culturing new varieties for inducing mutation of rhizome slice tissues of Aralia elata(Miq)Seem.. According to the method for culturing new varieties for inducing mutation of rhizome slice tissues of Aralia elata(Miq)Seem. disclosed by the invention, by using the rhizomes of the Aralia elata(Miq)Seem.as materials, the rhizomes are soaked with ethyl methanesulfonate with appropriate concentration for a certain time, then a plant tissue culturing technology is combined, and methods such as inductionof rhizome slice tissue buds (cormus) as well as rooting and rejuvenation growth of the buds (cormus) are adopted, so that the novel artificial cultured varieties of the Aralia elata(Miq)Seem. havingthe characteristics of being high in independence of each single plant, high in purity of genetic information of the single plant and clear in source of single plant can be obtained. The varieties are stable in genetic property, and can be applied to variety-line cultivation and building of mutant libraries of Aralia elata(Miq)Seem.
Owner:TONGHUA NORMAL UNIV +1
Rapid screening method of non-transgenic herbicide-tolerant rape
InactiveCN106258950AAvoid pollutionIncrease workloadPlant genotype modificationScreening methodPollen
The invention discloses a rapid screening method of non-transgenic herbicide-tolerant rape. Compared with the prior art, an M1 group mutated by EMS (Ethyl Methanesulfonate) is screened. A previous common flow of EMS mutation is that mutated M0 seeds are sowed to obtain M1, the M1 is harvested to obtain M2 segregating strains, and an M2 group is screened. The rape is an often cross-pollinated crop, and pollution caused by foreign pollen can be avoided when M1 single plants are bagged and self-crossed, and extremely great working amount is needed. However, a screened mutant target property is dominant, the M1 can be screened, and only extremely specific suspected single plants are further identified in the M2, so that the time is greatly shortened and the investment cost is reduced; large-scale screening becomes possible, and the rapid screening method has promotion and application value.
Owner:CHENGDU ACAD OF AGRI & FORESTRY SCI
A kind of method of ethyl methanesulfonate mutagenesis treatment Andrographis paniculata
ActiveCN111685036BConvenient researchImprove industrial efficiencySeed and root treatmentPlant genotype modificationBiotechnologyEthyl ester
Owner:CROP RES INST GUANGDONG ACAD OF AGRI SCI
A method for mutagenic treatment of Jatropha curcas seeds with ethyl methanesulfonate
InactiveCN103340150BAvoid the effects of large variations in germination rateShorten the timePlant genotype modificationRadicleEthylmethane Sulfonate
The invention discloses a method for mutagenizing barbadosnut seeds by ethylmethane sulfonate. The method disclosed by the invention comprises the following steps of: first, performing pre-bud forcing treatment at 30 DEG C on the barbadosnut seeds, selecting the seeds which are broken and exposed with unexpanded radicles after 3-7 days and soaking the seeds in a 0.9% EMS (Ethylmethane Sulfonate) liquor and soaking for 7 hours; forcing buds of the treated seeds at 28 DEG C and culturing for 3-4 days; and sowing mutagenized survival seeds in a soft peat soil substrate with radicles facing downward, and earthing by 1cm to breed. According to the method, the defects in the prior art are overcome, the mutagenizing concentration and time of EMS are reduced and the mutagenizing efficiency of the barbadosnut seeds by ethylmethane sulfonate is improved so as to facilitate selection by mutation of barbadosnut and promote industrialized development of the barbadosnut.
Owner:CROP RES INST GUANGDONG ACAD OF AGRI SCI
GC/FID determination method for contents of alkyl methanesulfonate and methanesulfonyl chloride in methanesulfonic acid
PendingCN112505188ALow costReach quantitative detectionComponent separationGas liquid chromatographicMethyl methanesulfonate
The invention discloses a GC / FID (Gas Chromatography / Flame Ionization Detector) determination method for contents of alkyl methanesulfonate and methanesulfonyl chloride in methanesulfonic acid (MsOH),namely methyl methanesulfonate (MMS), ethyl methanesulfonate (EMS), isopropyl methanesulfonate (IMS) and methanesulfonyl chloride (MsCl); and the method is characterized in that the methanesulfonic acid is subjected to water and dichloromethane extraction and layering treatment; gas chromatography and a hydrogen flame ion detector (GC / FID) are adopted, and the contents of methyl methanesulfonate,ethyl methanesulfonate, isopropyl methanesulfonate and methylsulfonyl chloride in methanesulfonic acid are detected quantitatively by adopting an internal standard method. According to the detectionmethod provided by the invention, the methyl methanesulfonate MMS, the ethyl methanesulfonate EMS, the isopropyl methanesulfonate IMS and the methylsulfonyl chloride MsCl are combined and detected inone method, so that the detection efficiency is improved, and the extraction method is simple, effective, high in sensitivity and good in recovery rate.
Owner:JIANGSU ALPHA PHARM CO LTD
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