37 results about "Ethyl methanesulfonate" patented technology

The invention relates to novel trans N-{4-{2-[4-(2, 3-dichlorophenyl)-piperazine-1-il]-ethyl}-cyclohexylamine dihydrochloride monohydrate and a process for the preparation of the trans N-{4-{2-[4-(2,3-dichlorophenyl)-piperazine-1-il]-ethyl}-cyclohexylamine dihydrochloride monohydrate, said process comprising the steps

• • a) reacting trans 2-{1-[4-(N-tert-butoxycarbonyl) amino]-cyclohexyl}-acetic acid ester with sodium borohydride and aluminium trichloride to give trans 2-{1-[4-(N-tert-butoxycarbonyl)-amino]-cyclohexyl}-ethanol;
  • b) reacting trans 2-{1-[4-(N-tert-butoxycarbonyl)-amino]cyclohexyl}-ethanol obtained with methanesulfonic acid chloride in the presence of an acid binding agent to give trans 2-{1-[4-(N-tert-butoxycarbonyl)-amino]-cyclohexyl}-ethyl methanesulfonate;
  • c) reacting trans 2-{1-[4-(N-tert-butoxycarbonyl)-amino]-cyclohexyl}-ethyl methanesulfonate obtained with 2,3-dichlorophenyl-piperazine in the presence of an acid binding agent to give trans 2-{1-[4-(N-tert-butoxycarbonyl)-amino]-cyclohexyl}-carbamic acid tert-butylester;
  • d) heating trans 2-{1-[4-(N-tert-butoxycarbonyl)-amino]-cyclohexyl}-carbamic acid tert-butylester obtained to a temperature between 40-100° C. in a mixture of aqueous hydrochloric acid/methanol to give trans N-{4-{2-[4-(2,3-dichlorophenyl) piperazine-1-il]-ethyl}-cyclohexylamine dihydrochloride monohydrate.

The invention belongs to the technical field of tobacco breeding and particularly relates to a large-scale efficient cultivation method for tobacco mutants on the basis of an EMS (ethyl methanesulfonate) mutagen. The method comprises steps of pretreatment of tobacco seeds, treatment with EMS, seed germination and transplanting, screening of variant plants as well as calculation of germination rateand survival rate. EMS treatment conditions are particularly optimized for main tobacco cultivar K326, the tobacco K326 is enabled to be sub-vital through probing, and the optimal EMS treatment conditions are obtained. The method can be applied to construction of a tobacco mutant library, can be directly applied to breeding of new varieties and has the advantage that advanced backcross is not needed, and genetic breeding resources of tobacco are enriched.

The invention discloses a breeding method for tetraploid maize. The breeding method comprises the steps of seed selection, seed germination culture, mutagenesis and seedling culture, wherein the mutagenesis step adopts chemical mutagenesis; the mutagenesis method of a maize tetraploid is high in mutagenesis success rate, and free of seedling rotting. A mutagen adopted in chemical mutagenesis comprises a mixture of colchicine, heteroauxin, periethylenenaphthalene, ethyl methanesulfonate, ethylenimine, nitroso-ethyl-urethane, nitroso-methyl-urethane, diethyl sulphate, azaserine, and mitomycin C. The mutagenesis method of the maize tetraploid is high in mutagenesis success rate, and free of seedling rotting, and has a wide market prospect; the chemical mutagen adopted in the mutagenesis method is obtained via precise proportioning, and can realize accurate induction of tetraploid maize; the treated maize is high in yield, short in growth cycle, and has competitive advantage; the breeding method is simple to operate, and easy for wide-range popularization.

Synthesis of chiral 2,2'-bi(diphenyl phosphine)-1,1'-binaphthalene is carried out by reacting 2,2'-bi(ethyl methanesulfonate trifluoroacetate)-1,1'-binaphthalene with diphenyl phosphate halogen under action of complex catalyst and auxiliaries to generate the final product. It's simple, has more yield and less by-products.

The invention provides a novel automobile accessory spraying technology. Cleaning, grinding and static electricity removal treatment is conducted on the surface of an automobile accessory; a coating material is prepared, ethyl methanesulfonate, 2-methacrylamide, nano-molybdenum disulfide, teflon, nano silicon dioxide, zinc powder, magnesium stearate and ethidene diamine are mixed, the temperatureis increased to 100-120 DEG C, and stirring is conducted for 25-35 min; then, under the power of 25-35 KW, ultrasonic treatment is conducted for 30-45 min; a medium-caliber spray gun is adopted to spray the coating material two times, the first-time spraying pressure is 0.6-0.7 MPa, the spraying distance is 15-25 cm, and the angle between the sprayed face and an automobile accessory plane is 65-75degrees; the second-time spraying pressure is 1.0-1.25 MPa, the spraying distance is 30-50 cm, the angle between the sprayed face and the automobile accessory plane is 80-90 degrees, and the intervaltime between two times of spraying is 8-12 min; and the sprayed automobile accessory can be obtained after grinding, drying and polishing treatment.

The invention relates to a cesium-137 mutagenesis breeding method for macadimia nuts. Macadimia nut seeds are subjected to sterilization treatment firstly, so that disease bacteria on the upper surfaces of the macadimia nut seeds can be killed, disease propagation is reduced, and field diseases are prevented. The seeds obtained after sterilization treatment are put into pretreatment liquid to be soaked, so that the effect of strengthening the chemical mutagenesis effect can be achieved, and chemical mutagenesis of the macadimia nut seeds is promoted. A chemical mutagenic agent is a mixture of ethyl methanesulfonate and diethyl sulfate, so that the mutation speed of the macadimia nut seeds can be increased, the mutation rate can be increased, and seeds with special characters can be obtained. A chemical mutagenesis treatment process is also limited and is divided into two times of mutagenesis treatment, so that the mutagenesis effect is better. Chemical mutagenesis treatment and 137Cs-gamma ray irradiation treatment are combined, so that the mutation speed of the macadimia nut seeds is further increased, the mutation rate is increased, and the seeds with special characters can be obtained. The seeds obtained after irradiation treatment are subjected to oscillation treatment, so that the mutation speed of the macadimia nut seeds can be further increased, and the mutation rate is increased.

The invention belongs to the field of crop seed breeding and in particular relates to a method for treating amaranth seeds by adopting ethyl methanesulfonate. According to the method provided by the invention, the amaranth seeds are selected as a starting receptor material for mutagenic treatment, and the amaranth seeds are subjected to the mutagenic treatment by utilizing an ethyl methanesulfonate solution with the concentration of 0 to 0.8 v/v percent; the amaranth seeds subjected to the mutagenic treatment are transferred into a culture dish paved with three layers of wet filter paper, andare continually cultured for several days under the conditions that the temperature is 20 to 25 DEG C, the illumination time is 12h/d and the intensity is 1500 to 2000lx; then the sprouted amaranth seeds are transferred into a hole disc with 72 holes and are continually cultured until the seeds grow to an M0 generation, and mutants are screened from the seeds. By adopting the method provided by the invention, the limitation of an existing seed breeding technology is overcome, and the mutagenic effect of the methanesulfonate on the amaranth seeds is improved; the method plays an active role onimprovement of the seed breeding efficiency of amaranths and promotion of sustainable development of an amaranth industry.

The invention discloses a short-day sensitive cell death SSD1 gene and a coded protein and application thereof. The SSD1 gene has a sequence as shown in SEQ ID NO:1. According to the invention, by using a model plant arabidopsis thaliana as a test material, through EMS (ethyl methanesulfonate) mutagenesis, an ssd1 mutant is obtained through screening. The SSD1 gene is separated by adopting a map-based cloning method, a gene mutational site is found, and the cell death is caused by deletion of the gene under the condition of short day. The SSD1 gene is necessary for the growth of arabidopsis thaliana under the condition of short day, and has a wide homology in plants. The finding of the biological function of the gene and the relevant research on a homologous gene are beneficial to deepening of the research on the controlling of the plant cell death by photoperiod, have important scientific significance in researching a molecular mechanism of the plant cell death, and also have important application value in improvement of crop varieties in a short-day region.

The invention discloses a mutagenesis method of a seed lotus phenotypic variation mutant plant. The mutagenesis method comprises the following steps of (1) shelling seed lotus seeds; (2) soaking the shelled lotus seeds in clear water to accelerate germination, and then cutting off part of cotyledons to expose germ growing points; (3) carrying out mutagenesis treatment by using an ethyl methanesulfonate solution as a mutagenic agent; and (4) performing rooting and germination in water, then performing field planting, and obtaining the mutant plant with phenotypic variation through phenotypic observation. A lotus EMS mutation system is explored by taking the seed lotus seeds as a material, and a result shows that shells of the lotus seeds are completely removed, so that the lotus seeds swelland germinate quickly and germinate orderly and consistently; and the shelled lotus seeds are soaked in clear water for 24 hours, 1/2-1/3 of the cotyledons are cut off, and the cotyledons are treatedin an EMS solution with the concentration of 0.5% for 16 hours to obtain mutant materials with various phenotypes, thereby providing a new germplasm material for seed-provided lotus breeding and effectively solving the problem of genetic stenosis of seed-provided lotus.

The invention relates to the technical field of drug analysis, and particularly relates to a method for detecting methanesulfonate genotoxic impurities in gemcitabine hydrochloride by a GC-MS/MS method. The method comprises the following steps of 1) adding 1, 2-dichloroethane into gemcitabine hydrochloride, performing ultrasonic treatment, adding 1, 2-dichloroethane to a constant volume to obtainan extracting solution, and filtering the extracting solution to obtain a test solution,2) determining the test solution in the step 1) by adopting a GC-MS/MS method, and calculating the content of each methanesulfonate genotoxic impurity in gemcitabine hydrochloride by adopting an external standard method, wherein the used gas chromatographic column is a VF-624ms chromatographic column, and the methanesulfonate genotoxic impurities comprise methyl methanesulfonate, ethyl methanesulfonate, isopropyl methanesulfonate, propyl methanesulfonate and butyl methanesulfonate. The determination methodprovided by the invention has the characteristics of strong impurity interference resistance, low noise, strong specificity and high sensitivity.

The invention provides a method for culturing sorghum seeds induced by ethyl methanesulfonate. The method comprises the following steps of (1) preparation of a seedling culture vessel; (2) preparationof a seedling culture medium, wherein the seedling culture medium is prepared, disinfected and air-dried; (3) preparation of a seedling culture site, wherein a layer of sand with the thickness of 1-2cm is laid on the upper portion of the ground of a sunlight glass greenhouse, and the lower layer is soil; (4) sowing; (5) seedling culture management; (6) transplanting, wherein seedlings are transplanted into a field when the seedling age is 35-50 days and the seedling height is 5 cm or above. According to the method, by carrying out greenhouse seedling culture on the seeds obtained after EMS mutagenesis, the emergence rate of mutagenetic offspring can be obviously increased, the maturity of mutagenetic single plants is accelerated, a nearly theoretical number of mutagenetic offspring materials are obtained, and accurate identification of the mutation characters of M1-generation mutagenetic plants is facilitated.

The invention discloses an oilseed rape mutation breeding method, and relates to the technical field of oilseed rape breeding, a test-tube plantlet cultured by a cabbage type oilseed rape young blank is adopted for mutagenesis, firstly, MS is used as a culture medium and is added into a mutagenesis culture medium containing 0.3% of ethyl methanesulfonate for mutagenesis culture, then C5H9NO2 and C5H9NO3 are used for two rounds of screening, and the mutagenesis of the oilseed rape is realized. According to the present invention, the enzyme activity of the HMP pathway of the mutant screened by C5H9NO2 and C5H9NO3 is strong, and the determination result of the glucose-6-phosphate dehydrogenase activity in the rapeseed forming process proves that the synthesis of fatty acid is easily achieved, such that the yield and the oil content quality of the rape are improved, the sclerotiniose resistance is enhanced, and the mature period is advanced.

The invention relates to a method for obtaining a fusarium oxysporum resistant carnation clone, belongs to the technical field of plant tissue culture, and aims at obtaining a fusarium oxysporum resistant breeding intermediate material by inducing calluses by utilizing carnation, mutagenizing by adopting ethyl methanesulfonate (EMS) and adding fusarium oxysporum toxin screening. According to the method, a large number of cells can be cultured and treated in a smaller space by utilizing a technology of adding pathogenic toxin into a culture medium to screen disease-resistant germplasm after clone mutation of the cells; direct induction and screening of mutants at the cellular level is an attempt of higher plant resistance breeding microbiotization; and the disease-resistant breeding periodcan be shortened, directional breeding is conducted, the selection efficiency is high, the effect is good, the field breeding period is shortened, and a new method is provided for disease-resistant breeding of carnation.

The invention discloses a breeding method for cellulase high-yield bacteria. The breeding method comprises the following steps: 1) culturing trichoderma viride and trichoderma reesei in a first medium, followed by 60 Co-gamma ray irradiation to obtain a mutant mixed mold; 2) culturing clostridium thermocellum in a second medium, followed by ultraviolet irradiation to obtain a mutant strain; 3) culturing the mutant mixed mold and the mutant strain in an MS medium to obtain a cellulase-producing strain, wherein the first medium contains nitrosoguanidine and diethyl sulfate; and the second mediumcontains ethyl methanesulfonate and diethyl sulfate. The cellulase high-yield bacteria selected by the method are resistant to degradation.

The invention discloses a preparation method of high-content sisomicin fermentation liquor, which belongs to the field of the medicine synthesis, and aims at solving the problems of the existing sisomicin fermentation liquor that the biological value is relatively low, the difference between a maximum value and a minimum value is great, the production is unstable, the content of a main component of the sisomicin is not high and the extraction and purification yield is low. The method comprises the steps: performing the activation by virtue of micromonospora inyoensis, then performing induced variation for an activated bacterial strain under the condition of ethyl methanesulfonate and microwaves, placing the mutated bacterial strain in a fermentation tank, promoting generation of sisomicin in a cobalt chloride solution, generating the sisomicin under the initiation of a specific bacterial liquid, and then filtering and separating to obtain the high-content sisomicin fermentation liquor. The purification yield is high.

The invention belongs to the technical field of plant propagation, in particular to a method for culturing new varieties for inducing mutation of rhizome slice tissues of Aralia elata(Miq)Seem.. According to the method for culturing new varieties for inducing mutation of rhizome slice tissues of Aralia elata(Miq)Seem. disclosed by the invention, by using the rhizomes of the Aralia elata(Miq)Seem.as materials, the rhizomes are soaked with ethyl methanesulfonate with appropriate concentration for a certain time, then a plant tissue culturing technology is combined, and methods such as inductionof rhizome slice tissue buds (cormus) as well as rooting and rejuvenation growth of the buds (cormus) are adopted, so that the novel artificial cultured varieties of the Aralia elata(Miq)Seem. havingthe characteristics of being high in independence of each single plant, high in purity of genetic information of the single plant and clear in source of single plant can be obtained. The varieties are stable in genetic property, and can be applied to variety-line cultivation and building of mutant libraries of Aralia elata(Miq)Seem.

The invention discloses a GC/FID (Gas Chromatography/Flame Ionization Detector) determination method for contents of alkyl methanesulfonate and methanesulfonyl chloride in methanesulfonic acid (MsOH),namely methyl methanesulfonate (MMS), ethyl methanesulfonate (EMS), isopropyl methanesulfonate (IMS) and methanesulfonyl chloride (MsCl); and the method is characterized in that the methanesulfonic acid is subjected to water and dichloromethane extraction and layering treatment; gas chromatography and a hydrogen flame ion detector (GC/FID) are adopted, and the contents of methyl methanesulfonate,ethyl methanesulfonate, isopropyl methanesulfonate and methylsulfonyl chloride in methanesulfonic acid are detected quantitatively by adopting an internal standard method. According to the detectionmethod provided by the invention, the methyl methanesulfonate MMS, the ethyl methanesulfonate EMS, the isopropyl methanesulfonate IMS and the methylsulfonyl chloride MsCl are combined and detected inone method, so that the detection efficiency is improved, and the extraction method is simple, effective, high in sensitivity and good in recovery rate.