Novel variety cultivation method for inducing petal mutation of physalis alkekengi l
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A cultivation method and technology for new varieties are applied in the field of cultivation of new varieties for inducing mutation of physalis petals, which can solve the problems of aphids, cabbage caterpillars, cotton bollworms, restrictions on development and utilization, small edible value and the like, and achieve low cultivation polymorphism. , the effect of shortening the breeding cycle and high mutation rate
Active Publication Date: 2020-06-19
通化天妍生物技术有限公司 +1
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[0004] In order to solve the above-mentioned Physalis fruit small, sour, low quality, poor disease resistance, little edible value and low yield, and Physalis common pests such as aphids, cabbage caterpillars, cotton bollworms, etc., resulting in its development and utilization are greatly affected Problems such as limitation, the present invention provides a kind of new variety breeding method of inducing Physalis petal mutation
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[0028] The technical solution in the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them.
[0029] Such as figure 1and 2 Shown, a kind of new variety cultivation method of inducing Physalis petal mutation, comprises the following steps:
[0030] 1 Materials and methods
[0031] 1.1 Material selection and pretreatment
[0032] In mid-June, after wild Physalis buds appear, select unopened full flower buds, remove the flower buds with flower stalks, rinse with 75% ethanol for 7 seconds, then move to saturated sodium hypochlorite solution for 4 minutes, rinse with sterile water 10 times, Blot the surface moisture with sterile filter paper for later use.
[0033] 1.2 Method
[0034] 1.2.1 Preparation of ethyl methanesulfonate (EMS) solution
[0035] EMS at a concentration of 0...
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Abstract
The invention belongs to the technical field of plant propagation, in particular to a novel variety cultivation method for inducing petal mutation of physalis alkekengi l. According to the novel variety cultivation method for inducing petal mutation of physalis alkekengi l, the cultivation method comprises the following steps: sterile petals are obtained through flower bud development and cultivation, pale green embryogenic calli are obtained by inducing embryogenic calli from the flower petals, the embryonic calli are induced by ethyl methanesulfonate to differentiate into embryoids with differences, the embryoids grow into individual plants and the like, so that a novel physalis alkekengi l cultivated variety with characteristics of strong independence, high purity of genetic informationand clear source and the like of the individual plants is obtained. The novel physalis alkekengi l cultivated variety is genetically stable, and can be utilized for strain cultivation and construction of physalis alkekengi l mutant library.
Description
technical field [0001] The invention belongs to the technical field of new plant varieties, and in particular relates to a method for cultivating new varieties by inducing mutations in Physalis petals. Background technique [0002] Physalis (Physalis alkekengi L.) is a perennial erect herbaceous perennial plant of the Solanaceae Physalis genus. Ghost lamps, etc., are eaten with fruits. Fruiting pedicels about 2-3cm long, mostly covered with persistent pilose; calyx ovoid, 2.5-4cm long, 2-3.5cm in diameter, thin leathery, prominent network veins, with 10 longitudinal ribs, orange or fiery red, covered with Persisting pilose, closed at the top, sunken at the base; berry spherical, orange-red, 10-15mm in diameter, soft and juicy. The flowering period is from May to September, and the fruiting period is from June to October. Physalis is highly adaptable, cold-resistant, heat-resistant, likes cool and humid climates, likes sunshine, and does not choose soil, especially Physali...
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