The invention belongs to the fields of paddy rice improvement and biological technology application, and specifically relates to a breeding method utilizing wild rice resource to create japonica-type novel paddy rice species. The method comprises the following steps: S1, extracting DNA genome from wild rice; S2, introducing DNA into paddy rice; S3, screening the plants with varied characters; S4, carrying out molecular verification on the screened variant plants. The invention provides a practicable and feasible way for creating novel paddy rice species. The method introduces a wild rice DNA into japonica-type rice so as to obtain an offspring material having wild rice characters of the japonica-type rice, and the introduction efficiency is as high as 6.8%. The method has the advantages of wide variation, wide selection population, shortened breeding period, suitability for breeding needs, and quick character stability, and the third generation of the introduced rice has stable varied characters. The created novel species can be used as a parent material for japonica rice breeding, or is subjected to a directed breeding to generate a novel species which can be applied to paddy rice production.