The invention relates to a filter device comprising: a first filter carrier (10) having x first optical filters (11, 12); and a second filter carrier (20) having y second optical filters (21-23), wherein x and y each independently have a value of at least 2, and wherein the filter carriers (10, 20) are each designed such that one filter (11, 12, 21-23) of each filter carrier (10, 20) can be positioned in an
optical path of the filter device. Each first
optical filter (11, 12) transmits light in a first
wavelength band (71, 72), and each second
optical filter (21-23) transmits light in x second
wavelength bands (81-86), wherein the first
wavelength bands (71, 72) do not overlap, the second wavelength bands (81-86) do not overlap, and each first
wavelength band (71, 72) contains a second
wavelength band (81-86) from each of the second optical filters (21-23). An optical analysis device comprises a
light source (41), a sample carrier (42), and an optical sensor (43). The filter device is arranged in an
optical path between the
light source (41) and the sample carrier (42) and / or in an
optical path between the sample carrier (42) and the optical sensor (43). In a method for operating the optical analysis device, a sample (50) is arranged on the sample carrier (42), is excited to fluoresce by means of the
light source (41), and light emitted by the sample (50) is detected by the optical sensor (43), wherein a
fluorescence analysis of the sample is carried out in x · y optical channels.