Breeding method utilizing wild rice resource to create japonica-type novel paddy rice species

Abstract

The invention belongs to the fields of paddy rice improvement and biological technology application, and specifically relates to a breeding method utilizing wild rice resource to create japonica-type novel paddy rice species. The method comprises the following steps: S1, extracting DNA genome from wild rice; S2, introducing DNA into paddy rice; S3, screening the plants with varied characters; S4, carrying out molecular verification on the screened variant plants. The invention provides a practicable and feasible way for creating novel paddy rice species. The method introduces a wild rice DNA into japonica-type rice so as to obtain an offspring material having wild rice characters of the japonica-type rice, and the introduction efficiency is as high as 6.8%. The method has the advantages of wide variation, wide selection population, shortened breeding period, suitability for breeding needs, and quick character stability, and the third generation of the introduced rice has stable varied characters. The created novel species can be used as a parent material for japonica rice breeding, or is subjected to a directed breeding to generate a novel species which can be applied to paddy rice production.

Description

technical field [0001] The invention belongs to rice genetic improvement and biotechnology application, in particular to a breeding method for creating new japonica rice germplasm by utilizing wild rice resources. Background technique [0002] Rice is an important food crop in the world. About 50% of the world's population uses rice as the staple food. my country is the world's largest producer and consumer of rice. The sown area and output occupy the first place in my country's food crops. During the process of rice domestication into cultivated rice and modern breeding of cultivated rice, many genes were lost due to selection pressure and the pursuit of high yield. The genetic basis of modern cultivated varieties is becoming increasingly narrow and single. Whether future breeding work can make continuous progress and breakthroughs is uncertain. Much depends on the utilization of germplasm resources. [0003] During the long-term natural selection process, wild rice has fo...

AI Technical Summary

Problems solved by technology

At present, the genetic basis of japonica rice cultivars is narrow and their stress resistance is poor. The hybridization of wild rice and japonica rice has problems such as poor flowering, poor fruiting, and infertility of hybrid offspring. It is difficult to improve japonica rice breeding by using wild rice. long

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