A new variety breeding method for inducing mutation of Physalis petals
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A breeding method and technology of new varieties, applied in the field of new variety cultivation of induced Physalis petal mutations, can solve the problems of aphids, cabbage caterpillars, cotton bollworms, development and utilization restrictions, and low edible value, and achieve low cultivation polymorphism , Shorten the breeding cycle, the effect of high mutation rate
Active Publication Date: 2022-03-08
通化天妍生物技术有限公司 +1
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[0004] In order to solve the above-mentioned Physalis fruit small, sour, low quality, poor disease resistance, little edible value and low yield, and Physalis common pests such as aphids, cabbage caterpillars, cotton bollworms, etc., resulting in its development and utilization are greatly affected Problems such as limitation, the present invention provides a kind of new variety breeding method of inducing Physalis petal mutation
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[0028] The technical solution in the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them.
[0029] Such as figure 1and 2 Shown, a kind of new variety cultivation method of inducing Physalis petal mutation, comprises the following steps:
[0030] 1 Materials and methods
[0031] 1.1 Material selection and pretreatment
[0032] In mid-June, after wild Physalis buds appear, select unopened full flower buds, remove the flower buds with flower stalks, rinse with 75% ethanol for 7 seconds, then move to saturated sodium hypochlorite solution for 4 minutes, rinse with sterile water 10 times, Blot the surface moisture with sterile filter paper for later use.
[0033] 1.2 Method
[0034] 1.2.1 Preparation of ethyl methanesulfonate (EMS) solution
[0035] EMS at a concentration of 0...
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Abstract
The invention belongs to the technical field of plant reproduction, and in particular relates to a method for cultivating a new variety of physalis petals induced mutation. The method for cultivating new varieties of Physalis physalis petal mutation induced by the present application is to obtain aseptic petals and embryogenic callus of petals by developing and cultivating flower buds, and to obtain light green embryogenic callus by inducing embryogenic callus. Ethyl acid ester mutagenesis and then differentiated culture pathways including differential embryoid bodies, embryoid body development into individual plants, etc., to obtain a new type of single plant with strong independence, high purity of genetic information of individual plants, and clear source of individual plants. The artificial cultivar of Physalis physalis is genetically stable and can be used as strain cultivation and construction of Physalis mutant library.
Description
technical field [0001] The invention belongs to the technical field of new plant varieties, and in particular relates to a method for cultivating new varieties by inducing mutations in Physalis petals. Background technique [0002] Physalis (Physalis alkekengi L.) is a perennial erect herbaceous perennial plant of the Solanaceae Physalis genus. Ghost lamps, etc., are eaten with fruits. Fruiting pedicels about 2-3cm long, mostly covered with persistent pilose; calyx ovoid, 2.5-4cm long, 2-3.5cm in diameter, thin leathery, prominent network veins, with 10 longitudinal ribs, orange or fiery red, covered with Persisting pilose, closed at the top, sunken at the base; berry spherical, orange-red, 10-15mm in diameter, soft and juicy. The flowering period is from May to September, and the fruiting period is from June to October. Physalis is highly adaptable, cold-resistant, heat-resistant, likes cool and humid climates, likes sunshine, and does not choose soil, especially Physali...
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