Rapid screening method of non-transgenic herbicide-tolerant rape

Abstract

The invention discloses a rapid screening method of non-transgenic herbicide-tolerant rape. Compared with the prior art, an M1 group mutated by EMS (Ethyl Methanesulfonate) is screened. A previous common flow of EMS mutation is that mutated M0 seeds are sowed to obtain M1, the M1 is harvested to obtain M2 segregating strains, and an M2 group is screened. The rape is an often cross-pollinated crop, and pollution caused by foreign pollen can be avoided when M1 single plants are bagged and self-crossed, and extremely great working amount is needed. However, a screened mutant target property is dominant, the M1 can be screened, and only extremely specific suspected single plants are further identified in the M2, so that the time is greatly shortened and the investment cost is reduced; large-scale screening becomes possible, and the rapid screening method has promotion and application value.

Description

technical field [0001] The invention belongs to the technical field of crop breeding, in particular to a rapid screening method for non-transgenic herbicide-resistant rapeseed. Background technique [0002] Weeds are a major disaster in agricultural production. They compete with crops for fertilizer, glory, and land, resulting in a decline in crop yield and quality, and have become a serious problem in agricultural production. Chemical weeding can not only effectively control the harm of weeds, increase the yield of crops, improve the quality of crops, but also liberate people from heavy agricultural labor and greatly improve labor efficiency. [0003] Imidazolinone herbicides are a class of broad-spectrum herbicides whose target is acetohydroxyacid synthase. At present, a series of resistant crops have been developed through mutagenesis and selection. Such resistant crops not only expand the application range of imidazolinone herbicides, but also prevent their damage to su...

AI Technical Summary

Problems solved by technology

[0002] Weeds are a major disaster in agricultural production. They compete with crops for fertilizer, glory, and land, resulting in a decline in crop yield and quality, and have become a serious problem in agricultural production.