Cesium-137 mutagenesis breeding method for macadimia nuts

A technology for mutation breeding and nut, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve problems such as single method, and achieve the effect of promoting chemical mutagenesis, speeding up mutation speed, and improving mutation rate

Active Publication Date: 2021-04-06
SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, macadamia nuts are usually bred by hybrid breeding, which is a single method, and it is of high research value to find breeding methods other than hybrid breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A cesium-137 mutation breeding method of macadamia nuts, comprising the following steps:

[0023] (1) Seed selection: Select mature shelled macadamia nuts with a diameter of 25㎜~30㎜, a kernel moisture content of 15.5%~20.0%, and a smooth and undamaged shell surface as seeds; the seeds can be picked in the same year, or It can be the seeds picked in the previous year, and it is not suitable to use seeds that have been stored for more than two years; in this implementation, the seeds picked in the current year are used, a total of 100;

[0024] (2) Seed pre-treatment: put the seeds into the sterilizing solution for 3 hours, and filter the water to obtain sterilized seeds; soak the sterilized seeds in the pre-treatment solution for 4 hours, and dry them in the shade to obtain the pre-treated seeds. Seeds; the pretreatment liquid uses water as a solvent, and contains ethanol with a concentration of 50g / kg, citric acid with a concentration of 20g / kg and sodium chloride with ...

Embodiment 2

[0033] A cesium-137 mutation breeding method of macadamia nuts, comprising the following steps:

[0034] (1) Seed selection: Select mature shelled macadamia nuts with a diameter of 25㎜~30㎜, a kernel moisture content of 15.5%~20.0%, and a smooth and undamaged shell surface as seeds; the seeds can be picked in the same year, or It can be the seeds picked in the previous year, and it is not suitable to use seeds that have been stored for more than two years; in this implementation, the seeds picked in the current year are used, a total of 100;

[0035] (2) Seed pre-treatment: put the seeds in the sterilizing solution for 5 hours, and filter the water with a filter to obtain sterilized seeds; put the sterilized seeds in the pre-treatment solution for 6 hours, and dry them in the shade to obtain the pre-treated seeds. Seeds; the pretreatment liquid uses water as a solvent, containing ethanol with a concentration of 100g / kg, citric acid with a concentration of 25g / kg and sodium chlo...

Embodiment 3

[0044] A cesium-137 mutation breeding method of macadamia nuts, comprising the following steps:

[0045] (1) Seed selection: Select mature shelled macadamia nuts with a diameter of 25㎜~30㎜, a kernel moisture content of 15.5%~20.0%, and a smooth and undamaged shell surface as seeds; the seeds can be picked in the same year, or It can be the seeds picked in the previous year, and it is not suitable to use seeds that have been stored for more than two years; in this implementation, the seeds picked in the current year are used, a total of 100;

[0046] (2) Seed pre-treatment: put the seeds into the sterilizing solution for 4 hours, and filter the water to obtain sterilized seeds; soak the sterilized seeds in the pre-treatment solution for 5 hours, and dry them in the shade to obtain the pre-treated seeds. Seed; the pretreatment liquid uses water as a solvent, and contains ethanol with a concentration of 80g / kg, citric acid with a concentration of 23g / kg and sodium chloride with a...

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Abstract

The invention relates to a cesium-137 mutagenesis breeding method for macadimia nuts. Macadimia nut seeds are subjected to sterilization treatment firstly, so that disease bacteria on the upper surfaces of the macadimia nut seeds can be killed, disease propagation is reduced, and field diseases are prevented. The seeds obtained after sterilization treatment are put into pretreatment liquid to be soaked, so that the effect of strengthening the chemical mutagenesis effect can be achieved, and chemical mutagenesis of the macadimia nut seeds is promoted. A chemical mutagenic agent is a mixture of ethyl methanesulfonate and diethyl sulfate, so that the mutation speed of the macadimia nut seeds can be increased, the mutation rate can be increased, and seeds with special characters can be obtained. A chemical mutagenesis treatment process is also limited and is divided into two times of mutagenesis treatment, so that the mutagenesis effect is better. Chemical mutagenesis treatment and 137Cs-gamma ray irradiation treatment are combined, so that the mutation speed of the macadimia nut seeds is further increased, the mutation rate is increased, and the seeds with special characters can be obtained. The seeds obtained after irradiation treatment are subjected to oscillation treatment, so that the mutation speed of the macadimia nut seeds can be further increased, and the mutation rate is increased.

Description

technical field [0001] The invention relates to the technical field of agricultural planting, in particular to a cesium-137 mutation breeding method for macadamia nuts. Background technique [0002] Macadamia nut alias: Queensland chestnut, macadamia walnut, macadamia nut, Queensland fruit, is a tree nut native to Australia. Macadamia nuts are evergreen trees and dicotyledonous plants. The crown is tall, with 3 to 4 leaves in whorls, lanceolate, leathery, smooth, with spiny serrations on the edges. Racemes axillary, beige flowers, spherical fruit, leathery, hard endocarp, beige to light brown kernels. It is suitable for growing in mild, humid and windy areas. Among the many dried fruits in the world, macadamia nuts have the highest economic value and have always enjoyed the reputation of "the king of dried fruits". The kernel of macadamia nuts is rich in nutrition (milky white), the exocarp is turquoise, the endocarp is hard and brown, the weight of a single fruit is 15-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 谭秋锦王文林宋海云韦媛荣汤秀华何铣扬郑树芳张涛韦哲君
Owner SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI
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