13 results about "Macadamia spp" patented technology

The invention relates to a macadamia nut flower and fruit retention synergistic method which comprises the following steps: in the early stage of flower bud differentiation, annularly applying slow-release granules to the root of a macadamia nut tree, and spraying fructan emulsion to the crown at the same time; in the early flowering period, a water dispersible sustained release preparation containing microencapsulated compound sodium nitrophenolate is sprayed to tree crowns of macadamia nuts, sodium alginate embedded borax sustained release microsphere suspension liquid is sprayed to leaf surfaces, and a hydrogel complexing agent containing sustained release monopotassium phosphate and amino acid is sprayed to the leaf surfaces; in the full-bloom stage, a composite slow-release emulsion containing liposome-embedded compound sodium nitrophenolate and brassinolide is sprayed to tree crowns of the macadamia nuts, slow-release gel microsphere suspension liquid of borax and amino acid is sprayed to leaf surfaces, and slow-release nano-emulsion containing beta-cyclodextrin-embedded bee wax volatile substances is sprayed to the tree crowns; in the initial fruit setting stage, sulfur-coated slow-release compound fertilizer is applied to the roots of macadamia nut trees, and ionic cross-linked calcium alginate microsphere embedded amino acid slow-release foliar fertilizer is sprayed to the leaf surfaces of the macadamia nut trees.

This invention discloses a soothing and repairing composition and its application, comprising the following raw materials: phytosterols, chamomile extract, hydrolyzed sodium hyaluronate, tocopherol, ceramide NP, triethanolamine, lactobacillus, macadamia seed oil, magnolia alcohol, matricaria flower extract, maltodextrin, lecithin, phosphatidylcholine, ascorbyl palmitate, inulin root juice, arginine / lysine polypeptide, squalane, jojoba seed oil, scutellaria baicalensis root extract, polygonum cuspidatum root extract, fructose, glycerin, p-hydroxyacetophenone, butylene glycol, acrylate / C10-30 alkanol acrylate crosspolymer, α-glucan oligosaccharide, 1,2-hexanediol, and water as the balance. Compared with the prior art, the soothing and repairing composition prepared by this invention has the effects of rapidly rebuilding the skin barrier, regulating skin immune function, improving skin microecology, synergistic effect, and rapid skin repair.

The invention belongs to the technical field of microbial prevention and control of plant diseases and insect pests, and particularly discloses a plant disease biocontrol bacillus cereus strain LCBC246 which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the strain is CGMCC No.33323. The invention further discloses a preparation method of the plant disease biocontrol bacillus cereus strain LCBC246. The LCBC246 strain provided by the invention is a gram-positive bacterium, has a very strong bacteriostatic effect on colletotrichum fucicola, the bacteriostatic rate is 92.1%, the disease control rate on the colletotrichum fucicola is 90.8%, and the LCBC246 strain has a very good inhibitory effect on other important plateau characteristic crop pathogenic bacteria and also has a remarkable crop growth promoting property. The bacterial inhibition rates of fermentation liquor and volatile substances of the strain LCBC246 on colletotrichum gloeosporioides of macadamia nuts are 62.70% and 76.58% respectively; the bacillus amyloliquefaciens can generate sterilization metabolites such as cellulase, amylase and siderophore, and has a very strong broad-spectrum bacteriostatic effect on various plant pathogenic fungi. Therefore, the strain LCBC246 and the metabolite thereof have the application value of developing bacterial biopesticides.

The application belongs to the technical field of molecular biology, and particularly relates to a macadamia SNP molecular marker combination and application thereof. The SNP molecular marker combination comprises 284 SNP sites, the 284 SNP sites can uniformly cover a macadamia genome, have high polymorphism information content, and can be used for constructing a macadamia DNA fingerprint map, and on the basis of the DNA fingerprint map, the SNP molecular marker combination can be further used for identifying macadamia germplasm resources, and can solve problems such as mixed germplasm in resources, repeated preservation and management, and intellectual property right disputes. Meanwhile, the 284 SNP sites are used for screening macadamia core germplasm, so that the most abundant genetic diversity of a species can be maximally represented by the least number of germplasm and the lowest genetic redundancy, the management and utilization efficiency of the germplasm resources is improved, the genetic resources are effectively protected and utilized, and useful genetic resources are selected for a macadamia breeding plan.

The application discloses an application of a macadamia germplasm HAES826 in breeding of a new macadamia variety with self-compatibility and high-quality fruits, and belongs to the technical field of plant breeding. The application discloses that the macadamia germplasm HAES826 has excellent traits of self-compatibility and low fruit quality degradation rate of offspring, and can be used as a core breeding parent to obtain self-compatible and high-quality varieties through hybrid breeding or pure line breeding. The bagging selfing fruit setting rate of the macadamia germplasm HAES826 is greater than or equal to 0.5%, and the selfing fruit weight, total amount of unsaturated fatty acids and total amount of essential amino acids of the selfing fruits have a selfing degradation rate of less than 20%, 10% and 10%, respectively. The application can solve the problem that self-compatibility and high-quality traits are difficult to be considered in macadamia breeding, significantly shorten the breeding cycle, and cultivate varieties with reduced orchard pollination management cost, stable yield and high quality, and outstanding industrial application value.

The application provides a method for improving drought resistance of macadamia rootstock by overexpressing MiNAC2a gene, and belongs to the technical field of planting, and the method comprises the following steps: obtaining cotyledon of aseptic seedling from macadamia seed, inducing callus by using the cotyledon of the aseptic seedling, constructing an overexpression vector according to the obtained MiNAC2a gene sequence, transferring the vector into agrobacterium, mediating the agrobacterium to transfer into macadamia callus by injection method, culturing positive callus to grow adventitious buds and adventitious roots, extracting DNA of the seedling to verify whether the positive plant is true or not by detecting bands, hardening the seedling, transplanting the seedling to a field, obtaining transgenic drought-resistant rootstock resources, strengthening the root system of the transgenic seedling, and improving the drought resistance of the rootstock. The technology uses transgenic technology to cross boundaries, break through reproductive isolation, and broaden the gene resource library, and provides a train of thought for precisely introducing key drought-resistant genes in bacteria, fungi and even other drought-resistant plants into macadamia trees.

The invention belongs to the technical field of agricultural planting, and particularly relates to a method for increasing the fruit setting number of macadamia nuts, which comprises the following steps: separating selected macadamia nut female parent and male parent plants by using an insect-proof net before flowering so as to avoid the influence of insects; after the female parents enter a full-bloom stage, selecting sunny weather with the daytime air temperature of 20-30 DEG C and the wind speed of less than 1m / s, cleaning original pollen on styluses of the female parents by using a sterile writing brush, brushing the collected pollen of the male parents on the styluses by using a brush pen for pollination, keeping isolation for 14 days after pollination, and then removing the insect-proof net. The high-affinity pollination combination is screened by combining germplasm such as Guijin No.1, Guijin A4 and Guijin YM26 with pollen viability. The optimal pollination combination is screened by evaluating the affinity of 16 hybrid combinations of 10 germplasms of macadamia nuts. Results show that when A4 is used as a male parent and Guihot No.1 is used as a female parent, the affinity of cross pollination is the highest, the success rate of cross pollination is remarkably improved, the average fruit setting number is remarkably increased, and effective technical support is provided for high-yield cultivation of macadamia nuts.

The present application relates to the field of agricultural planting technology, and particularly relates to a method for improving the germination rate of macadamia seed. The method for improving the germination rate of macadamia seed comprises the following steps: (1) drying the shelled macadamia seed for 5-10 days; (2) cold storing the dried macadamia seed of step (1) at 0-10 DEG C for 270-300 days; (3) sterilizing the cold stored macadamia seed of step (2); and (4) performing germination treatment on the sterilized macadamia seed of step (3). The method is simple, only cold storage treatment is performed on the macadamia seed, and the germination rate of the treated macadamia seed can reach 92.9%, which significantly improves the germination rate of the macadamia seed, enhances the germination ability of the seed, and shortens the germination time of the seed.

The application discloses a method for promoting the formation and growth of macadamia roots. The method comprises the following steps: first, cultivating the macadamia seedlings with a vegetative growth solution A, irrigating each seedling with 200-400 mL of the vegetative growth solution A every 2-3 days, and continuing for 9-10 days; second, cultivating the seedlings with a nutrient mixed solution after the cultivation with the vegetative growth solution A is completed, irrigating each seedling with 300-400 mL of the nutrient mixed solution every 2-3 days, and continuing for 25-30 days; the nutrient mixed solution comprises 40-100 micromoles per liter of naphthalene acetic acid (NAA), 10-40 micromoles per liter of strigolactone, and a vegetative growth solution B; and after the cultivation period of the nutrient mixed solution is completed, the macadamia seedlings can form abundant macadamia roots, which is beneficial to the absorption of the nutrients in the soil by the macadamia. The method combines the vegetative growth solution, the strigolactone and the naphthalene acetic acid in the growth process, promotes the root formation ability of the macadamia, and increases the formation of the macadamia roots.

The invention discloses a method for interplanting pleurotus citrinopileatus under a macadamia nut forest. The method comprises the following steps: constructing a standardized cultivation-management circulating unit; waste of the macadamia nut industry is used as a core raw material, and the culture medium is prepared according to the dry weight volume; paving the culture medium into the culture furrows, and then inoculating pleurotus citrinopileatus cultispecies by adopting a layer sowing or mixed sowing mode; an orchard drip irrigation system is started for humidity management, and nutrition is supplemented in the mushroom cultivation period after each flush of mushrooms is harvested; when pleurotus citrinopileatus sporocarp pilei are bright golden yellow, timely harvesting by adopting a rotating pulling method; after 2-3 times of mushrooms are harvested, mushroom bran which is rich in hypha protein and completely decomposed in the cultivation furrows and covering soil are turned over and buried into the deep position of the orchard soil on the spot. According to the method, a forest-fungus interplanting mode is adopted, the under-forest space ecological niche and fruit shells, branches and other solid wastes generated by the method are integrated and utilized, large-scale and wild-imitating cultivation of pleurotus citrinopileatus is conducted, and collaborative symbiosis and cyclic development of cultivating fungi with forests and promoting forests with fungi are achieved.

The present application belongs to the field of plant biotechnology and molecular breeding technology, and particularly relates to a drought resistance early identification method for macadamia based on multi-omics markers and application thereof. The present application detects the expression amount of MiWRKY22, MiNAC29 and MiP5CS genes in the leaves of the macadamia to be tested, and the content of proline and quercetin, substitutes the detection data into a drought resistance index (DRI) calculation model, and determines the drought resistance grade of the plant according to the DRI value. The present application integrates transcription and metabolic information into a quantifiable evaluation index for the first time, can be identified under non-stress conditions at the seedling stage, has the advantages of early identification, accuracy and high throughput, is suitable for macadamia germplasm resource evaluation, parent selection and early generation screening of breeding, and significantly improves the drought resistance breeding efficiency.

The application discloses a kind of planting management methods that can reduce physiological fruit drop of macadamia in karst area, and relates to the technical field of fruit tree planting management.The application embeds the mixture of composite water-retaining material and decomposed organic fertilizer in the hole dug at drip line during the flowering period of macadamia, sprays plant growth regulator, photosynthetic recovery agent and fruit retention fertilizer successively after 7-10 days of thanks to flower, and simultaneously root applies water-soluble high-potassium fertilizer and removes new shoots and a series of technical means, solves the problems that hormone combination and spraying fruit retention fertilizer are difficult to reduce physiological fruit drop of macadamia in karst area and difficult to increase fruit weight in the prior art.Using the management method of the application, the cumulative fruit drop rate in the early stage can be reduced from about 75% of the conventional method to 62-65%, the cumulative fruit drop rate in the later stage can be reduced from about 85% of the conventional method to 65-68%, and the size of fruit can be increased, and the weight of single fruit can be increased.

The invention relates to self-stabilization type oil-water double-soluble transparent essence oil and a preparation method thereof, and the essence oil comprises the following components by weight: 11-25% of macadamia nut seed oil, 1-3% of Candida bee / glucose / rapeseed methyl oleate fermentation product, 10-20% of plant extract composite liquid, and the balance of caprylic acid / capric triglyceride and 1, 2-hexanediol in a mass ratio of (0.9-1.2): 1. Wherein the plant extraction compound liquid is an aqueous solution of a plant extraction compound, and the plant extraction compound is a composition of a cockscomb extract, an andrographis paniculata leaf extract and a campsis grandiflora extract. The essence oil realizes a self-stabilization transparent system without a traditional emulsifier, can stably contain 10-20% of plant extraction composite liquid, has high-content water-soluble active matters, stable and transparent oil properties and good skin compatibility, also realizes repairing and anti-inflammatory effects, and avoids the problem of weak effect of single plant extraction.