23 results about "Macadamia spp" patented technology

The invention relates to a method for creating and identifying excellent drought-enduring germplasm of macadamia nuts. The method comprises the following steps: (1) selecting seeds; (2) seed soaking treatment; (3) chemical mutagenesis; (4) performing ray irradiation mutagenesis; (5) raising seedlings; (6) culturing in a culture medium; (7) identifying selected products; more germplasm with various shapes can be obtained under the combination of chemical mutagenesis and low, high and medium three-level ray irradiation mutagenesis, and then a germplasm culture medium is optimized so as to identify and screen excellent drought-resistant macadamia germplasm; the first culture medium is optimized, meanwhile, the temperature and the impermeability of the culture medium are controlled, and macadimia nut germplasm with the better drought resisting effect can be preferably screened out; the second culture medium is optimized, then small molecule active substances are added, meanwhile, parameters such as impermeability of the culture medium are controlled, macadamia nut germplasm with the better drought resistance effect is further screened out, macadamia nut seedling root growth can be effectively promoted, and the drought resistance of macadamia nut seedlings can be conveniently calculated according to the rooting amount of seedling root systems.

The invention relates to a macadamia nut flower and fruit retention synergistic method which comprises the following steps: in the early stage of flower bud differentiation, annularly applying slow-release granules to the root of a macadamia nut tree, and spraying fructan emulsion to the crown at the same time; in the early flowering period, a water dispersible sustained release preparation containing microencapsulated compound sodium nitrophenolate is sprayed to tree crowns of macadamia nuts, sodium alginate embedded borax sustained release microsphere suspension liquid is sprayed to leaf surfaces, and a hydrogel complexing agent containing sustained release monopotassium phosphate and amino acid is sprayed to the leaf surfaces; in the full-bloom stage, a composite slow-release emulsion containing liposome-embedded compound sodium nitrophenolate and brassinolide is sprayed to tree crowns of the macadamia nuts, slow-release gel microsphere suspension liquid of borax and amino acid is sprayed to leaf surfaces, and slow-release nano-emulsion containing beta-cyclodextrin-embedded bee wax volatile substances is sprayed to the tree crowns; in the initial fruit setting stage, sulfur-coated slow-release compound fertilizer is applied to the roots of macadamia nut trees, and ionic cross-linked calcium alginate microsphere embedded amino acid slow-release foliar fertilizer is sprayed to the leaf surfaces of the macadamia nut trees.

This invention discloses a soothing and repairing composition and its application, comprising the following raw materials: phytosterols, chamomile extract, hydrolyzed sodium hyaluronate, tocopherol, ceramide NP, triethanolamine, lactobacillus, macadamia seed oil, magnolia alcohol, matricaria flower extract, maltodextrin, lecithin, phosphatidylcholine, ascorbyl palmitate, inulin root juice, arginine / lysine polypeptide, squalane, jojoba seed oil, scutellaria baicalensis root extract, polygonum cuspidatum root extract, fructose, glycerin, p-hydroxyacetophenone, butylene glycol, acrylate / C10-30 alkanol acrylate crosspolymer, α-glucan oligosaccharide, 1,2-hexanediol, and water as the balance. Compared with the prior art, the soothing and repairing composition prepared by this invention has the effects of rapidly rebuilding the skin barrier, regulating skin immune function, improving skin microecology, synergistic effect, and rapid skin repair.

The invention belongs to the technical field of agricultural planting, and particularly relates to a method for increasing the fruit setting number of macadamia nuts, which comprises the following steps: separating selected macadamia nut female parent and male parent plants by using an insect-proof net before flowering so as to avoid the influence of insects; after the female parents enter a full-bloom stage, selecting sunny weather with the daytime air temperature of 20-30 DEG C and the wind speed of less than 1m / s, cleaning original pollen on styluses of the female parents by using a sterile writing brush, brushing the collected pollen of the male parents on the styluses by using a brush pen for pollination, keeping isolation for 14 days after pollination, and then removing the insect-proof net. The high-affinity pollination combination is screened by combining germplasm such as Guijin No.1, Guijin A4 and Guijin YM26 with pollen viability. The optimal pollination combination is screened by evaluating the affinity of 16 hybrid combinations of 10 germplasms of macadamia nuts. Results show that when A4 is used as a male parent and Guihot No.1 is used as a female parent, the affinity of cross pollination is the highest, the success rate of cross pollination is remarkably improved, the average fruit setting number is remarkably increased, and effective technical support is provided for high-yield cultivation of macadamia nuts.

The invention belongs to the technical field of microbial prevention and control of plant diseases and insect pests, and particularly discloses a plant disease biocontrol bacillus cereus strain LCBC246 which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the strain is CGMCC No.33323. The invention further discloses a preparation method of the plant disease biocontrol bacillus cereus strain LCBC246. The LCBC246 strain provided by the invention is a gram-positive bacterium, has a very strong bacteriostatic effect on colletotrichum fucicola, the bacteriostatic rate is 92.1%, the disease control rate on the colletotrichum fucicola is 90.8%, and the LCBC246 strain has a very good inhibitory effect on other important plateau characteristic crop pathogenic bacteria and also has a remarkable crop growth promoting property. The bacterial inhibition rates of fermentation liquor and volatile substances of the strain LCBC246 on colletotrichum gloeosporioides of macadamia nuts are 62.70% and 76.58% respectively; the bacillus amyloliquefaciens can generate sterilization metabolites such as cellulase, amylase and siderophore, and has a very strong broad-spectrum bacteriostatic effect on various plant pathogenic fungi. Therefore, the strain LCBC246 and the metabolite thereof have the application value of developing bacterial biopesticides.

The application belongs to the technical field of molecular biology, and particularly relates to a macadamia SNP molecular marker combination and application thereof. The SNP molecular marker combination comprises 284 SNP sites, the 284 SNP sites can uniformly cover a macadamia genome, have high polymorphism information content, and can be used for constructing a macadamia DNA fingerprint map, and on the basis of the DNA fingerprint map, the SNP molecular marker combination can be further used for identifying macadamia germplasm resources, and can solve problems such as mixed germplasm in resources, repeated preservation and management, and intellectual property right disputes. Meanwhile, the 284 SNP sites are used for screening macadamia core germplasm, so that the most abundant genetic diversity of a species can be maximally represented by the least number of germplasm and the lowest genetic redundancy, the management and utilization efficiency of the germplasm resources is improved, the genetic resources are effectively protected and utilized, and useful genetic resources are selected for a macadamia breeding plan.

The present invention belongs to the field of microbial technology, and specifically relates to a synthetic bacterial community for preventing and treating macadamia nut decay disease, and a construction method and application thereof. An application of a synthetic bacterial community in preventing and treating macadamia nut decay disease, wherein the synthetic bacterial community is formed by mixing equal volumes of Bacillus subtilis X17, Pseudomonas fluorescens X62 and Trichoderma harzianum Z27; the pathogen of the macadamia nut decay disease is Pseudomonas J1 and / or Cladosporium J2. The present invention isolates and screens strains from healthy rhizosphere soil, and conducts a pairwise confrontation test on the well-growing strains to screen out three strains that have no antagonistic effect between the two and have an inhibitory effect on the pathogen of macadamia nut decay disease. Molecular biological identification determines that the three strains are: Bacillus subtilis X17, Pseudomonas fluorescens X62 and Trichoderma harzianum Z27, and then the three strains are constructed into a synthetic bacterial community. It is verified by potted plant experiments that the synthetic bacterial community constructed by the present invention can effectively prevent and treat macadamia nut decay disease, and can provide support for the planting and economic development of macadamia nuts.

The invention relates to a method for effectively reducing the fungus bag pollution rate of oyster mushroom cultivated by using macadamia nut peel and bagasse as raw materials, and belongs to the technical field of edible fungus cultivation. The method comprises the steps of compost preparation, earlier-stage pretreatment of compost, bagging, sterilization, inoculation, mycelium culture and the like. Macadamia nut peel and bagasse are used as main raw materials for cultivation of oyster mushrooms, the macadamia nut peel is mixed with a small amount of lime in advance for pre-wetting and composting for 24 h, then the macadamia nut peel is mixed with bagasse and wood dust for pre-wetting for 24 h, and finally bran, gypsum and residual lime are added. After bagging, the bag is sterilized at normal pressure at 100 DEG C or above and kept for 16 hours, so that the pollution rate of the fungus bag in the mycelium culture period can be effectively reduced. The method disclosed by the invention solves the problem of high fungus bag pollution rate caused by slow water absorption and incomplete pre-wetting after the macadamia nut peels are dried.

The invention provides a composition containing plant seed grease and a preparation method thereof. The plant seed oil is selected from simmondsia chinensis seed oil, safflower seed oil, meadowfoam seed oil, sunflower seed oil, macadamia nut seed oil, castor seed oil, peony seed oil, grape seed oil, grapefruit seed oil, apple seed oil, pomegranate seed oil, watermelon seed oil, zucchini seed oil, linseed oil, rapeseed oil, sesame seed oil and the like or a combination thereof. Based on the total weight of the composition, the composition comprises at least 0.5 wt% of plant seed grease. The invention also relates to an application of the composition containing the plant seed grease in enhancing the skin barrier function and / or in preparing a medicine for enhancing the skin barrier function and / or an external preparation for the skin.

The application discloses an application of a macadamia germplasm HAES826 in breeding of a new macadamia variety with self-compatibility and high-quality fruits, and belongs to the technical field of plant breeding. The application discloses that the macadamia germplasm HAES826 has excellent traits of self-compatibility and low fruit quality degradation rate of offspring, and can be used as a core breeding parent to obtain self-compatible and high-quality varieties through hybrid breeding or pure line breeding. The bagging selfing fruit setting rate of the macadamia germplasm HAES826 is greater than or equal to 0.5%, and the selfing fruit weight, total amount of unsaturated fatty acids and total amount of essential amino acids of the selfing fruits have a selfing degradation rate of less than 20%, 10% and 10%, respectively. The application can solve the problem that self-compatibility and high-quality traits are difficult to be considered in macadamia breeding, significantly shorten the breeding cycle, and cultivate varieties with reduced orchard pollination management cost, stable yield and high quality, and outstanding industrial application value.

The application provides a method for improving drought resistance of macadamia rootstock by overexpressing MiNAC2a gene, and belongs to the technical field of planting, and the method comprises the following steps: obtaining cotyledon of aseptic seedling from macadamia seed, inducing callus by using the cotyledon of the aseptic seedling, constructing an overexpression vector according to the obtained MiNAC2a gene sequence, transferring the vector into agrobacterium, mediating the agrobacterium to transfer into macadamia callus by injection method, culturing positive callus to grow adventitious buds and adventitious roots, extracting DNA of the seedling to verify whether the positive plant is true or not by detecting bands, hardening the seedling, transplanting the seedling to a field, obtaining transgenic drought-resistant rootstock resources, strengthening the root system of the transgenic seedling, and improving the drought resistance of the rootstock. The technology uses transgenic technology to cross boundaries, break through reproductive isolation, and broaden the gene resource library, and provides a train of thought for precisely introducing key drought-resistant genes in bacteria, fungi and even other drought-resistant plants into macadamia trees.

A method for improving the survival rate of seedling grafting and promoting well-developed roots, comprising the following steps: (1) selecting budstock seedlings; (2) pre-treating the budstock seedlings; (3) pre-treating the elite mother trees and selecting grafting buds; (4) grafting; (5) managing the grafted seedlings. Before grafting the budstock seedlings and before taking grafting buds from the elite mother trees, they are pre-treated with the budstock seedling pre-treatment solution and the elite mother tree pre-treatment solution respectively. After grafting, a grafting healing agent is coated around the grafting incision. After one month of grafting, a root-promoting fertilizer is applied to the roots. When raising seedlings by the method of the present invention, the seedling age is smaller, the survival rate is over 95%, the emergence time is short, and the macadamia grafted seedlings grow vigorously and have good growth potential during subsequent field planting.

The invention belongs to the technical field of agricultural planting, and particularly relates to a method for increasing the fruit setting number of macadamia nuts, which comprises the following steps: separating selected macadamia nut female parent and male parent plants by using an insect-proof net before flowering so as to avoid the influence of insects; after the female parents enter a full-bloom stage, selecting sunny weather with the daytime air temperature of 20-30 DEG C and the wind speed of less than 1m / s, cleaning original pollen on styluses of the female parents by using a sterile writing brush, brushing the collected pollen of the male parents on the styluses by using a brush pen for pollination, keeping isolation for 14 days after pollination, and then removing the insect-proof net. The high-affinity pollination combination is screened by combining germplasm such as Guijin No.1, Guijin A4 and Guijin YM26 with pollen viability. The optimal pollination combination is screened by evaluating the affinity of 16 hybrid combinations of 10 germplasms of macadamia nuts. Results show that when A4 is used as a male parent and Guihot No.1 is used as a female parent, the affinity of cross pollination is the highest, the success rate of cross pollination is remarkably improved, the average fruit setting number is remarkably increased, and effective technical support is provided for high-yield cultivation of macadamia nuts.

The utility model discloses a cultivation device for screening drought-enduring germplasm of macadamia nut, the cultivation device for screening drought-enduring germplasm of macadamia nut adopts a cultivator, the cultivator is provided with a cultivation groove, a reservoir and a plurality of gutters, the gutters are arranged at intervals along the circumference of the cultivator, and the gutters are communicated with the reservoir and the cultivation groove; water supply cotton of the water supply assembly can be contained in any water guide groove, at least part of the water supply cotton extends into the water storage pool and the culture groove, and the water supply cotton is used for guiding a solution in the water storage pool into the culture groove. And the spray header is connected to the culture device and is positioned above the culture tank. According to the cultivation device for screening the drought-enduring germplasm of the macadamia nuts, the relative humidity of the matrix can be kept within a constant range numerical value, so that an operator can conveniently cultivate and screen the drought-enduring germplasm of the macadamia nuts to a great extent.

The present invention belongs to the field of microbial technology, and specifically relates to a synthetic bacterial community that promotes the growth of macadamia nuts, and a construction method and application thereof. A synthetic bacterial community, the synthetic bacterial community is formed by mixing equal volumes of Bacillus subtilis X17, Pseudomonas fluorescens X62 and Trichoderma harzianum Z27. The present invention separates and screens strains from healthy rhizosphere soil, and conducts a pairwise confrontation test on the well-growing strains to screen out strains that have no antagonistic effect between the two. Through molecular biological identification, three strains with growth-promoting effects are screened, namely: Bacillus subtilis X17, Pseudomonas fluorescens X62 and Trichoderma harzianum Z27, and then the three strains are constructed into a synthetic bacterial community. It is verified by potted plant experiments that the synthetic bacterial community constructed by the present invention can promote the growth of macadamia nut plants and the accumulation of biomass, and can also activate the soil, improve the soil nutrient content, enzyme activity and microbial biomass carbon, nitrogen and phosphorus content, and can provide support for the planting and economic development of macadamia nuts.

The application provides a salt-tolerant bacillus YH30 and application thereof, and relates to the technical field of microbial prevention and treatment. Bacillus halotolerans The salt-tolerant bacillus YH30 is classified as bacillus halodurans , and was preserved in the China General Microbiological Culture Collection Center on December 2, 2024, with a preservation number of CGMCC No: 32877; and the salt-tolerant bacillus YH30 is applied to the prevention and treatment of grapefruit penicillium disease, blueberry fruit botrytis disease and macadamia leaf anthracnose. The application overcomes the defects of the prior art, can effectively control the damage of grapefruit penicillium disease, blueberry fruit botrytis disease and macadamia leaf anthracnose, and promotes the improvement of the resistance level of grapefruit fruit, blueberry fruit and macadamia leaf, and has a positive promoting effect on the sustainable and healthy development of grape, blueberry and macadamia planting industry.

The present application relates to the field of agricultural planting technology, and particularly relates to a method for improving the germination rate of macadamia seed. The method for improving the germination rate of macadamia seed comprises the following steps: (1) drying the shelled macadamia seed for 5-10 days; (2) cold storing the dried macadamia seed of step (1) at 0-10 DEG C for 270-300 days; (3) sterilizing the cold stored macadamia seed of step (2); and (4) performing germination treatment on the sterilized macadamia seed of step (3). The method is simple, only cold storage treatment is performed on the macadamia seed, and the germination rate of the treated macadamia seed can reach 92.9%, which significantly improves the germination rate of the macadamia seed, enhances the germination ability of the seed, and shortens the germination time of the seed.

The present invention belongs to the technical field of plant molecular breeding and germplasm identification, and specifically relates to a macadamia SNP molecular marker and its application. Specifically, the present invention uses resequencing data of 208 macadamia germplasms to identify and screen core SNP markers in the whole genome. Based on the core SNP markers, the DNA fingerprint map and phylogenetic tree of the above macadamia germplasm are constructed, which provides data reference for the management, utilization and new variety registration of macadamia germplasm resources, and therefore has good practical application value.

The application discloses a method for promoting the formation and growth of macadamia roots. The method comprises the following steps: first, cultivating the macadamia seedlings with a vegetative growth solution A, irrigating each seedling with 200-400 mL of the vegetative growth solution A every 2-3 days, and continuing for 9-10 days; second, cultivating the seedlings with a nutrient mixed solution after the cultivation with the vegetative growth solution A is completed, irrigating each seedling with 300-400 mL of the nutrient mixed solution every 2-3 days, and continuing for 25-30 days; the nutrient mixed solution comprises 40-100 micromoles per liter of naphthalene acetic acid (NAA), 10-40 micromoles per liter of strigolactone, and a vegetative growth solution B; and after the cultivation period of the nutrient mixed solution is completed, the macadamia seedlings can form abundant macadamia roots, which is beneficial to the absorption of the nutrients in the soil by the macadamia. The method combines the vegetative growth solution, the strigolactone and the naphthalene acetic acid in the growth process, promotes the root formation ability of the macadamia, and increases the formation of the macadamia roots.

The invention discloses a method for interplanting pleurotus citrinopileatus under a macadamia nut forest. The method comprises the following steps: constructing a standardized cultivation-management circulating unit; waste of the macadamia nut industry is used as a core raw material, and the culture medium is prepared according to the dry weight volume; paving the culture medium into the culture furrows, and then inoculating pleurotus citrinopileatus cultispecies by adopting a layer sowing or mixed sowing mode; an orchard drip irrigation system is started for humidity management, and nutrition is supplemented in the mushroom cultivation period after each flush of mushrooms is harvested; when pleurotus citrinopileatus sporocarp pilei are bright golden yellow, timely harvesting by adopting a rotating pulling method; after 2-3 times of mushrooms are harvested, mushroom bran which is rich in hypha protein and completely decomposed in the cultivation furrows and covering soil are turned over and buried into the deep position of the orchard soil on the spot. According to the method, a forest-fungus interplanting mode is adopted, the under-forest space ecological niche and fruit shells, branches and other solid wastes generated by the method are integrated and utilized, large-scale and wild-imitating cultivation of pleurotus citrinopileatus is conducted, and collaborative symbiosis and cyclic development of cultivating fungi with forests and promoting forests with fungi are achieved.

The present application belongs to the field of plant biotechnology and molecular breeding technology, and particularly relates to a drought resistance early identification method for macadamia based on multi-omics markers and application thereof. The present application detects the expression amount of MiWRKY22, MiNAC29 and MiP5CS genes in the leaves of the macadamia to be tested, and the content of proline and quercetin, substitutes the detection data into a drought resistance index (DRI) calculation model, and determines the drought resistance grade of the plant according to the DRI value. The present application integrates transcription and metabolic information into a quantifiable evaluation index for the first time, can be identified under non-stress conditions at the seedling stage, has the advantages of early identification, accuracy and high throughput, is suitable for macadamia germplasm resource evaluation, parent selection and early generation screening of breeding, and significantly improves the drought resistance breeding efficiency.

The application discloses a kind of planting management methods that can reduce physiological fruit drop of macadamia in karst area, and relates to the technical field of fruit tree planting management.The application embeds the mixture of composite water-retaining material and decomposed organic fertilizer in the hole dug at drip line during the flowering period of macadamia, sprays plant growth regulator, photosynthetic recovery agent and fruit retention fertilizer successively after 7-10 days of thanks to flower, and simultaneously root applies water-soluble high-potassium fertilizer and removes new shoots and a series of technical means, solves the problems that hormone combination and spraying fruit retention fertilizer are difficult to reduce physiological fruit drop of macadamia in karst area and difficult to increase fruit weight in the prior art.Using the management method of the application, the cumulative fruit drop rate in the early stage can be reduced from about 75% of the conventional method to 62-65%, the cumulative fruit drop rate in the later stage can be reduced from about 85% of the conventional method to 65-68%, and the size of fruit can be increased, and the weight of single fruit can be increased.

The invention relates to self-stabilization type oil-water double-soluble transparent essence oil and a preparation method thereof, and the essence oil comprises the following components by weight: 11-25% of macadamia nut seed oil, 1-3% of Candida bee / glucose / rapeseed methyl oleate fermentation product, 10-20% of plant extract composite liquid, and the balance of caprylic acid / capric triglyceride and 1, 2-hexanediol in a mass ratio of (0.9-1.2): 1. Wherein the plant extraction compound liquid is an aqueous solution of a plant extraction compound, and the plant extraction compound is a composition of a cockscomb extract, an andrographis paniculata leaf extract and a campsis grandiflora extract. The essence oil realizes a self-stabilization transparent system without a traditional emulsifier, can stably contain 10-20% of plant extraction composite liquid, has high-content water-soluble active matters, stable and transparent oil properties and good skin compatibility, also realizes repairing and anti-inflammatory effects, and avoids the problem of weak effect of single plant extraction.