20 results about "Fructan" patented technology

The application discloses a method for screening garlic fructan adversity response key enzyme genes and metabolites based on transcriptome and metabolome, and the method can better analyze the relationship between genes and metabolites by jointly analyzing the fructan-related differentially expressed genes measured by the transcriptome and the fructan-related metabolism substances with obvious metabolism changes measured by the metabolome, and constructing a gene-metabolite network interaction diagram.

The invention relates to a macadamia nut flower and fruit retention synergistic method which comprises the following steps: in the early stage of flower bud differentiation, annularly applying slow-release granules to the root of a macadamia nut tree, and spraying fructan emulsion to the crown at the same time; in the early flowering period, a water dispersible sustained release preparation containing microencapsulated compound sodium nitrophenolate is sprayed to tree crowns of macadamia nuts, sodium alginate embedded borax sustained release microsphere suspension liquid is sprayed to leaf surfaces, and a hydrogel complexing agent containing sustained release monopotassium phosphate and amino acid is sprayed to the leaf surfaces; in the full-bloom stage, a composite slow-release emulsion containing liposome-embedded compound sodium nitrophenolate and brassinolide is sprayed to tree crowns of the macadamia nuts, slow-release gel microsphere suspension liquid of borax and amino acid is sprayed to leaf surfaces, and slow-release nano-emulsion containing beta-cyclodextrin-embedded bee wax volatile substances is sprayed to the tree crowns; in the initial fruit setting stage, sulfur-coated slow-release compound fertilizer is applied to the roots of macadamia nut trees, and ionic cross-linked calcium alginate microsphere embedded amino acid slow-release foliar fertilizer is sprayed to the leaf surfaces of the macadamia nut trees.

The present invention relates to the use of cellobiofructose (CBF) as a dietary fiber and / or prebiotic component in food products, food product additives, nutraceuticals and / or beverages or for increasing the growth of probiotic microorganisms. The invention further relates to CBF and compositions containing it for use in the prevention and / or treatment of obesity, constipation, high cholesterol, diabetes, hypertension, and / or heart disease. The invention further encompasses a method for the (recombinant) production of cellobiofructose (CBF), as described herein, using a polypeptide having levansucrase activity which comprises or consists of a polypeptide having at least 80 % sequence identity to the amino acid sequence set forth in SEQ ID NO: 1.

The application discloses ecdysterone-low molecular weight fructan composite microspheres with fat-reducing and muscle-increasing functions, and a preparation method and application thereof, and belongs to the technical field of biological medicine and functional food. The composite microspheres have a core-shell structure, the inner core is a calcium alginate gel, the outer shell is chitosan, the inner core co-encapsulates ecdysterone and low molecular weight fructan, and the mass ratio of the two is 0.8-1.2:1. The preparation adopts a liquid drop microfluidic technology combined with an internal gelation method to form the inner core, and adopts a post-crosslinking method to coat the outer shell. The composite microspheres are regular in shape, uniform in particle size, high in encapsulation rate, good in stability, excellent in pH response slow-release and anti-degradation characteristics in the gastrointestinal tract. In-vivo pharmacological experiments prove that the composite microspheres can reduce the body weight and body fat rate of obese mice, increase the lean meat content and muscle strength, realize synergistic effects of fat reduction and muscle increase, and can be used for preparing drugs or functional food for improving obesity-related muscle loss, low-muscle obesity and related metabolic syndrome.

The invention discloses a method for increasing the alcohol content of fermented mash of broomcorn millet yellow rice wine, which comprises the following steps: inoculating saccharomyces cerevisiae into a liquid seed culture medium, culturing to obtain a saccharomyces cerevisiae seed solution, inoculating the saccharomyces cerevisiae seed solution into a Levan fructan liquid culture medium, culturing for a period of time, and then carrying out ultrasonic treatment to obtain the alcohol content of the fermented mash of broomcorn millet yellow rice wine. After the ultrasonic treatment is finished, obtaining saccharomyces cerevisiae strain fermentation liquor; inoculating saccharomyces cerevisiae strain fermentation liquor obtained after ultrasonic treatment into broomcorn millet mash for fermentation, and obtaining broomcorn millet yellow wine fermentation mash with high alcohol content after fermentation is finished. The invention provides a technical means for improving the quality such as flavor and mellowness of the broomcorn millet yellow rice wine, can further improve the production efficiency of the broomcorn millet yellow rice wine, save the production cost and improve the economic benefit of broomcorn millet yellow rice wine enterprises, has important economic value and practical significance, and is worthy of popularization and application. And technical support is provided for high-quality development and market competitiveness improvement of the husked millet yellow wine industry.

The application discloses a kind of planting methods for improving the content of Dendrobium candidum polysaccharide, covers seedling pretreatment, substrate preparation, light temperature water stress regulation, microbial reinforcement, selenium-silicon nutrition supplement and intelligent harvesting decision.By red light / blue light combination (3:1) and pulse drought activation secondary metabolism, inoculate beta-fructan endophyte Paenibacillus polymyxa and add chelated selenium-silicon foliar fertilizer, combined with low temperature and low humidity adversity in the later growth stage, the stem polysaccharide content reaches 38%-42%.System integration LED light source, EC sensing irrigation and bacterial agent spraying equipment, realize standardization production.Embodiment shows that the method is 35-40% higher than traditional planting polysaccharide, and there is no gene editing risk, in line with GAP specification.

The present invention relates to a composition comprising a fructan, a resistant starch and psyllium. The present invention also relates to a method of increasing fibre consumption in a subject in need thereof, comprising administering the composition to the subject. Furthermore, the present invention relates to a method of: (i) promoting growth of short chain fatty acid producing bacteria in the gastrointestinal tract of a subject in need thereof; (ii) increasing short chain fatty acid production in the gastrointestinal tract of a subject in need thereof; and / or (iii) reducing the potential side effects associated with the consumption of fibre by a subject in need thereof; comprising administering the composition to the subject. Uses of the composition and methods of preparing the composition are also discussed. 33 20 25 20 08 73 07 F eb 2 02 5 A B S T R A C T 2 0 2 5 2 0 0 8 7 3 0 7 F e b 2 0 2 5 3 3

The present invention relates to a composition, foodstuff, food supplement, or pharmaceutical composition comprising butyric acid or a derivative thereof and agave fructan, and its use for maintaining and / or enhancing proper gastrointestinal function. Figure for the abstract: Fig. 1

The invention discloses active fructan. The fructan is prepared from ginseng, poria cocos and bighead atractylodes rhizome powder in a classic famous formula for the first time, and the activity of the fructan is verified. Particularly relates to preparation of fructan and application of fructan in medicines and health-care foods for treating obesity T2DM, and belongs to the technical field of traditional Chinese medicines. Extraction and purification, structural analysis and activity verification prove that fructan has a relatively strong effect of improving obesity T2DM. The fructan is newly found from the ginseng, poria cocos and rhizoma atractylodis macrocephalae powder, has relatively good activity in the aspect of improving the obesity T2DM function, is small in side effect, low in price and relatively good in druggability, and can be used for preparing the medicine for improving the obesity T2DM function independently or in combination with other medicines. The compound can be prepared into granules, capsules, tablets and other dosage forms with pharmaceutically or food acceptable carriers or excipients.

The present invention relates to a prebiotic composition for use in a method for maintaining or improving the health of a subject in need thereof, comprising at least one inulin-type fructan (ITF) and at least one human milk oligosaccharide (HMO), wherein the subject in need thereof is an HMO non-responder; a food, a dietary supplement or a drink comprising a prebiotic composition for use in a method for maintaining or improving the health of a subject in need thereof, wherein the subject in need thereof is an HMO non-responder; and an inulin-type fructan (ITF) for use in a method for maintaining or improving the health of a subject in need thereof by administering a prebiotic composition comprising at least one human milk oligosaccharide (HMO), wherein the subject in need thereof is an HMO non-responder.The present invention also relates to the use of said prebiotic composition as a dietary supplement, food or drink for prebiotic non-responders. The present invention also relates to a prebiotic composition comprising at least one inulin-type fructan (ITF) and at least one human milk oligosaccharide (HMO) for use in a method for the treatment of a mood disorder, preferably depression or anxiety, wherein a subject in need thereof is an HMO non-responder.

The invention relates to the technical field of plant polysaccharide extracts, in particular to a fructan polysaccharide substance derived from rhizoma atractylodis macrocephalae and application of the fructan polysaccharide substance in preparation of anti-aging drugs. According to the fructan polysaccharide substance AMP, the AMP is neutral fructan, a main chain is formed by alternating 1, 2-connected beta-D-fructofuranose, 2, 6-connected beta-D-Fuf and 1, 2, 6-connected beta-D-Fuf, and a side chain is selected from one or more of alpha-L-arabinofuranose, 1, 3-connected alpha-D-glucopyranose and beta-D-Fuf. The chemical structural characteristics and properties of AMP, including functional groups, monosaccharide composition, molecular weight, spatial conformation, crystallization characteristics and stability, are analyzed. The AMP activates a spleen GPR41 / GPR43 signal channel, promotes T / B lymphocyte proliferation and relieves the G1 phase retardation of a cell cycle by improving the generation of intestinal short-chain fatty acid, especially butyric acid, so that the imbalance of peripheral lymphocyte subpopulations is corrected, tissue aging and DNA damage markers are reduced, and the purposes of recovering immune homeostasis and delaying immune decline are achieved.

The invention discloses levan sucrase as well as a mutant, a recombinant expression vector and application thereof, and belongs to the technical field of bioengineering. The amino acid sequence of the fructan sucrase is as shown in SEQ ID NO.2 or SEQ ID NO.4, and the amino acid sequence of the fructan sucrase mutant is as shown in SEQ ID NO.8. The invention further discloses a preparation method of the fructan sucrase mutant. The recombinant expression vector contains the coding gene of the levan sucrase or the mutant of the levan sucrase. The invention also discloses application of the fructan sucrase and the mutant thereof in hydrolysis of cane sugar or synthesis of kestose. The specific activity of the fructan sucrase is 66.17 U / mg, a fructan sucrase mutant with higher enzyme activity and higher thermal stability is obtained after mutation modification of the fructan sucrase, and the specific activity of the fructan sucrase mutant is 169.37 U / mg. The fructan sucrase and the mutant thereof can be used for hydrolysis of cane sugar and synthesis of kestose, and have a good application prospect.

The invention relates to a low-sugar fruit juice beverage and a production process thereof, and the production process of the low-sugar fruit juice beverage comprises the following preparation steps: S1, raw material pretreatment; s2, mixing ingredients; s3, blending core components; s4, dispersing a stabilizer; s5, performing homogenizing treatment; s6, sterilizing and cooling; s7, filtering and filling; the composite granules and the composite sol are added into a low-sugar formula, a natural functional component and dietary fiber dual nutrition system is constructed, and the composite granules take high-purity natural theanine as a core material and are embedded by a fructan wall material, so that the effects of soothing nerves and improving sleep are reserved, and reaction with other components is avoided; the composite sol is derived from blueberry pomace and celery juice, is rich in natural dietary fibers, can overcome the defects that traditional low-sugar fruit juice is single in nutrition, insufficient in function and the like, and all functional components are natural sources and accord with the clean label consumption trend that the components are extremely simple, natural and traceable.

The present application relates to a kind of uniform fructan GEIN and its application, belong to food processing technical field.The uniform fructan GEIN described in the present application has similar spherical structure of starch granule, can significantly improve the pasting properties of Gastrodia elata starch.And, the uniform fructan GEIN described in the present application also has the effect of improving intestinal flora, can promote the growth of beneficial bacteria in intestinal tract, while not cause postprandial hyperglycemia.The discovery and isolation of the uniform fructan GEIN described in the present application fill the research blank of the starch pasting regulation effect of Gastrodia elata polysaccharide, provide new active ingredient for the depth development of Gastrodia elata in the field of food and medicine, and have important significance for the development of Gastrodia elata food and health care product.

The invention belongs to the technical field of medical biogel preparation, and discloses medical biogel of microbial fructan and a preparation method of the medical biogel. The medical biogel of the microbial fructan comprises a hydroxyl functionalized non-woven fabric base layer, the gel layer is compounded with the base layer; the gel layer is of a three-dimensional network structure formed by crosslinking methylacryloylation modified microbial fermentation fructan and polylipoic acid through covalent bonds. The activity of sulfur free radicals at the tail end of the modified microbial fermentation fructan molecule is quenched through combination of a double bond on the modified microbial fermentation fructan molecule and a disulfide bond in lipoic acid, so that the lipoic acid-based material is more stable, and the adhesive force is improved. The medical biological glue based on the polylipoic acid can be effectively adhered to the surfaces of various human tissues, and can release lipoic acid molecules with antibacterial activity, so that the potential of the medical biological glue as an active medical biological glue is shown. And finally, by taking the cellulose non-woven fabric as a template, gelation is performed on the cellulose non-woven fabric, so that the overall material is endowed with excellent mechanical strength and biocompatibility.

The application discloses tea oil ice cream and a preparation method thereof, and relates to the technical field of food preparation, and comprises the following steps: purifying fructan and rhamnus frangula leaf pectin; dissolving the purified compound pectin and xanthan gum in ultrapure water to form a compound polysaccharide solution; mixing tea oil and the compound polysaccharide solution to prepare an oil gel; mixing the oil gel with skimmed milk powder, white granulated sugar, egg yolk, gellan gum and pure water, sterilizing, homogenizing and aging, and then adding the mixture into an ice cream machine to process tea oil ice cream. The application has the beneficial effects that tea oil is ingeniously applied in ice cream, thereby expanding the application range of tea oil; the oil gel prepared by using polysaccharide does not need a surfactant, and the hardness of the oil gel is lower and the oil separation rate is lower due to the use of pectin in the polysaccharide; and the ice cream prepared by using the oil gel has better expansion rate and melting resistance compared with the ice cream prepared by using butter.

The invention discloses polygonatum kingianum polysaccharide and a preparation method thereof, the polygonatum kingianum polysaccharide is composed of fructose and glucose in a molar ratio of 18.23: 1, is a Graminan type fructan, and has a structure shown as a formula I; the preparation method comprises the following steps: (1) carrying out alcohol extraction and impurity removal, water extraction and alcohol precipitation, redissolution and freeze drying on polygonatum kingianum powder to obtain polygonatum kingianum water extraction crude polysaccharide; (2) performing combined deproteinization by a papain-Sevage method, and dialyzing to obtain polygonatum kingianum refined polysaccharide; (3) carrying out primary separation by adopting DEAE-cellulose DE-52 anion exchange chromatographic column chromatography to obtain polygonatum kingianum polysaccharide washing component powder; and (4) carrying out purification by using a Sephadex G-100 gel chromatographic column, so as to obtain the purified polygonatum kingianum polysaccharide. Polygonatum kingianum polysaccharide can be used for preparing medicines, foods or functional foods with the effects of resisting fatigue and regulating intestinal flora.

This invention relates to a β-2,6-fructan sucrase mutant specifically for the production of high molecular weight L-glycans and its applications. LimosiLactobacillus reuteri A mutant β-2,6-fructan sucrase, N275W, was obtained by mutating asparagine at position 275 of its amino acid sequence to tryptophan. Under optimal catalytic conditions, the mutant completely lost its ability to generate oligosaccharides and significantly enhanced its ability to synthesize polysaccharides. This discovery is of significant research value for studying β-2,6-fructan sucrase enzymes that can produce L-fructans of specific chain lengths.

The application belongs to the technical field of medical biological glue preparation, and discloses a medical biological glue of microbial fructan and a preparation method thereof. The medical biological glue of microbial fructan comprises a hydroxyl-functionalized non-woven fabric base layer and a gel layer compounded with the base layer; the gel layer is a three-dimensional network structure formed by covalent bond cross-linking of methyl acryloyl-modified microbial fermentation fructan and polysulfated fatty acid. The combination of the double bond on the modified microbial fermentation fructan molecule and the disulfide bond in the sulfated fatty acid quenches the terminal sulfur radical activity, so that the sulfated fatty acid-based material is more stable and the adhesion is improved. The medical biological glue based on polysulfated fatty acid can effectively adhere to various human tissue surfaces and release sulfated fatty acid molecules with antibacterial activity, showing the potential as an active medical biological glue. Finally, the cellulose non-woven fabric is used as a template to perform gelation thereon, so that the material as a whole has excellent mechanical strength and biocompatibility.

The invention provides application of recombinant fructan sucrase in catalyzing EGCG (epigallocatechin gallate) glycosylation reaction, an obtained product and application. The gene sequence of the recombinant fructan sucrase is shown as SEQ ID NO: 1. The enzyme can efficiently catalyze EGCG with high regioselectivity, through process optimization, the conversion rate reaches 65% or above, the enzyme method is mild in process condition and good in selectivity, the water solubility of the product is remarkably improved compared with that of an EGCG raw material, meanwhile, the antioxidant activity is reserved, the enzyme method shows clear advantages in the aspect of improving inherent defects (poor solubility and instability) of EGCG, the bioavailability of the enzyme is improved, and the enzyme method is suitable for industrial production. A core raw material and a feasible method are provided for developing new products with high stability and high bioavailability in the fields of functional foods, medicines, cosmetics and the like.