Fructan-rich fruit and vegetable juice beverage and preparation method thereof
A technology for fruit and vegetable juice and fructan, which is applied to sugary food ingredients, food preparation, bacteria used in food preparation, etc., can solve problems such as artificial addition, and achieve the effects of saving production costs, reducing pollution risks, and having a wide range of sources.
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Embodiment 1
[0029] Embodiment 1 is rich in the preparation of the fruit and vegetable juice drink of fructan
[0030] 1. Materials and methods
[0031] Preparation of tomato juice sucrose medium: ripe tomatoes were washed, peeled, squeezed by a juicer, filtered through 100-mesh gauze to get the juice, boiled for 5 minutes, centrifuged at 8000g for 10 minutes to get the supernatant, added 15% (w / v) sucrose and heated to dissolve Cool to room temperature, adjust the pH to 6.5 with food-grade alkali, sterilize at 121°C for 20 minutes, and cool to room temperature to obtain a sterile tomato juice sucrose medium.
[0032] Preparation of seeds (fermentation strains): the lyophilized powder of Leuconostoccitreum (Leuconostoccitreum) BD01707 whose preservation number is CGMCCNo. / v) On the M17 agar medium (MerckCo. Germany) of sucrose, 28 ℃ of aerobic cultures were taken out for 24 hours, and a single colony was picked with an inoculation loop and put into 20 mL of M17 liquid medium (MerckCo.) c...
Embodiment 2
[0060] Embodiment 2 is rich in the preparation of the fruit and vegetable juice drink of fructan
[0061] 1. Materials and methods
[0062] Preparation of tomato juice sucrose medium: ripe tomatoes were washed, peeled, squeezed by a juicer, filtered through 100-mesh gauze to get the juice, boiled for 10 minutes, centrifuged at 4000g for 12 minutes to take the supernatant, added 30% sucrose by mass, heated to dissolve and then cooled to room temperature, adjust the pH to 7.5 with food-grade alkali, sterilize at 135°C for 10 minutes, and cool to room temperature to obtain a sterile tomato juice sucrose medium.
[0063] The preparation of seeds (fermentation strains), the preparation of polysaccharides (fructans) in the fermentation broth, and the preparation of polysaccharides (fructans) in fruit and vegetable juice beverages: the same as in Example 1.
[0064] 2. Preparation of fruit and vegetable juice beverage rich in fructan
[0065] (1) Aseptically inoculate the obtained ...
Embodiment 3
[0068] Embodiment 3 is rich in the preparation of the fruit and vegetable juice beverage of fructan
[0069] 1. Materials and methods
[0070] Preparation of tomato juice sucrose medium: ripe tomatoes were cleaned, peeled, squeezed by a juicer, filtered through 120-mesh gauze to get the juice, boiled for 1 min, centrifuged at 12000 g for 8 min to take the supernatant, added 5% sucrose by mass, heated to dissolve and then cooled to room temperature, adjust the pH to 5.5 with food-grade alkali, sterilize at 110°C for 30 minutes, and cool to room temperature to obtain a sterile tomato juice sucrose medium.
[0071] The preparation of seeds (fermentation strains), the preparation of polysaccharides (fructans) in the fermentation broth, and the preparation of polysaccharides (fructans) in fruit and vegetable juice beverages: the same as in Example 1.
[0072] 2. Preparation of fruit and vegetable juice beverage rich in fructan
[0073] (1) Aseptically inoculate the obtained Leucono...
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