199results about How to "Promote proliferation and differentiation" patented technology

The invention discloses a polypeptide composition for facial care. The polypeptide composition comprises the following components in percentage by mass: 0.0001-0.0005% of acetyl hexapeptide-8, 0.01-0.1% of acetyl tetrapeptide-5, 0.02-0.1% of tripeptide-1 copper, 0.0005-0.1% of glutathione, 0.0001-0.0005% of oligopeptide-1, 0.0001-0.0005% of oligopeptide-2, 0.0001-0.0005% of oligopeptide-3, 0.0001-0.0005% of oligopeptide-4, 0.0001-0.0005% of oligopeptide-5, 1.0-5.0% of polyethylene glycol, 84.697-97.9689% of mannitol and 1.0-10.0% of deionized water. The polypeptide composition disclosed by the invention comprises 9 polypeptide active matters, has the advantages of remedying and whitening skin and preventing aging, and is capable of permeating into skin for improving blood circulation of the face, supplying sufficient nutrients and energy to cells and promoting metabolism of the skin, and thus the purposes of remedying and caring skin can be achieved; and a freeze-drying technique is adopted in a production process, so that various active substances can be retained to the maximum extent, original properties of various active substances can be maintained, conversion of active matters can be prevented, and the stability of a product can be improved.

The invention discloses an anti-aging tightening eye mask with tuber magnatum. Essence for the anti-aging tightening eye mask with the tuber magnatum comprises, by weight, 2-6% of butanediol, 2-5% ofglycerin, 0.1-1% of tuber magnatum extract, 1-5% of grape fruit extract, 1-5% of chamomile extract liquid, 0.5-3% of corn protein amino acids, 0.5-2% of hibiscus esculentus fruit extract, 0.5-2% of biological carbohydrate gum-1, 0.5-1.5% of tremella fuciformis polysaccharides, 0.5-1% of D-panthenol, 1-3% of oligopeptides-1, 0.03-0.1% of sodium hyaluronate, 0.1-0.3% of allantoin, 0.5-3% of auxiliary components and the balance deionized water. An eye mask bag with a built-in non-woven fabric is filled with the essence for the anti-aging tightening eye mask with the tuber magnatum to obtain the anti-aging tightening eye mask with the tuber magnatum. The anti-aging tightening eye mask has the advantages that components in the anti-aging tightening eye mask are matched with one another, accordingly, skin can be multi-dimensionally repaired from diversified aspects such as wrinkle resistance, moisturizing, oxidation resistance, inflammation diminishing and antiviral action and cell proliferation and differentiation promotion, and the anti-aging tightening eye mask can assist in solving problems of eye skin loosening, eye wrinkle, under-eye puffiness and the like for the skin.

The present invention provides a method for treating osteoporosis in a subject in need of such treatment, including administering orally to the subject an effective amount of an extract product of a Dioscorea species capable of promoting proliferation and differentiation of bone marrow cells in the subject, wherein the Dioscorea species is characterized by a randomly amplified polymorphic DNA (RAPD) fingerprint including 14 DNA bands ranging from 428 bp, 452 bp, 537 bp, 602 bp, 723 bp, 817 bp, 934 bp, 1140 bp, 1242 bp, 1478 bp, 1641 bp, 1904 bp, 2151 bp and 2918 bp, respectively, when genomic DNA of the Dioscorea species is amplified with a primer of SEQ ID NO: 9, and the extract product is prepared by a process including the steps of: (a) extracting a tuber of the Dioscorea species using an alcohol-based solvent in the presence of acetic acid; (b) subjecting the resultant product obtained in step (a) to a separating treatment to obtain a soluble fraction; and (c) removing solvent from the soluble fraction obtained in step (b) extract fractions of Dioscorea sp.

The invention discloses a method for forming a porous bone hydroxyapatite ceramic cytoskeleton based on a photo-curing 3D printing technology. The method mainly includes the five steps of ball-millingbone hydroxyapatite powder, mixing a pore forming agent, preparing photo-curing resin slurry, forming a ceramic blank through photo-curing, and degreasing and sintering the blank. The technological steps are simple, a complicated skeleton structure which cannot be formed through a traditional method can be formed, and the cytoskeleton similar to a human skeleton in structure and component can beprepared through a hollowed-out topological structure, especially a porous structure with two types of apertures.

The invention discloses a method for preparing a three-dimensional porous stent composite layer, and belongs to the technical field of biomaterials. In order to prevent inflammatory reaction caused by drop of scraps of a stent due to a dynamic action, a coating film is required on the surface of a porous ceramic stent, and addition of hydroxyapatite powder fills up a voidness defect of the surface layer of the stent so as to improve the strength of the stent; a porous calcium phosphate ceramic stent is compounded by a polylactic acid solution added with hydroxyapatite and a growth factor-loaded hydrogel solution; growth factors loaded in the hydrogel can be slowly released to a human part needing the growth factors under the joint action of own diffusion and slow degradation of polymeric hydrogel so as to promote vascular endothelial growth of a tissue and proliferation and differentiation of an osteoblast; and the stent is gradually degraded and eventually transformed into a bone; and the method is mainly used for preparing the three-dimensional porous stent composite layer.

The invention relates to a double-layer collagen dermal scaffold and a preparation method thereof; the double-layer collagen dermal scaffold comprises a collagen layer and a silicone layer; the collagen layer is a collagen sponge layer, and the silicone layer is a silicone mixture layer or a pretreated silicone mixture layer; the collagen layer induces cell migration and promotes proliferation and differentiation so as to accelerate the recovery of an affected part; along with the degrading of material, regenerated tissues integrated to tissues around are finally formed; the silicone layer is semipermeable membrane, which is capable of controlling water loss of the affected part, protecting the wound and avoiding infection, has good mechanical strength and helps suture and fix the double-layer collagen dermal scaffold; by combining collagen and the silicone layer, it is possible to accelerate the growth of granulation tissues; by controlling collagen concentration and pH value to control the degrading rate of the collagen layer, the silicone layer can be removed smoothly after the granulation tissue phase; by transplanting auto-epidermis, healing cycle can be shortened, healing quality can be improved, and scars are decreased.

The invention discloses a surface modified titanium or titanium alloy material. The surface of the material is provided with at least five layers of bioactive coats consisting of double-layer chitosan-silk fibroin. The material has favorable osteoplast compatibility, can promote multiplication and division of osteoplast, shows potential for further developing a novel medical hard tissue implant material, and has favorable clinical application prospect. The surface modified titanium or titanium alloy material can be prepared by a self-assembly method layer by layer; alternate layers of polycation electrolyte chitosan and polyanion electrolyte gelatin are laminated and assembled on the surface of the titanium or titanium alloy material through electrostatic adsorption action; and the preparation method is simple, can control coating thickness, is not affected by size and shape of the material, is suitable for the materials with complex appearance, has moderate preparation conditions, needs no special equipment, and has low production cost, strong practicability, and better popularization and application values.

The invention discloses a biological ink used for 3D biological printing and a preparation method thereof. The biological ink comprises the following components in percentage by mass: 8%-20% of a hydrophilic polymer with the crosslinking function, 10-20% of a water-soluble enzyme with the crosslinking function, 0.1%-1% of bacterial cellulose, and the balance a sterile PBS solution. The biologicalink can be used for 3D printing after mixed with cells, and the shear thinning properties of the used materials make the biological ink have the characteristics of self-supporting after extrusion. Theused materials have good cell compatibility, the enzyme crosslinking agent forms a hydrogel at physiological temperature (about 37 DEG C), the reaction conditions are mild, the cells are not damaged,a hydrogel support has relatively good stability in a culture medium, and thereby cell proliferation can be supported.

The invention belongs to the technical field of skin care products, and particularly relates to mask liquid with a repairing function and a preparation method thereof in order to solve the problems that in the prior art, flushing, red itching and slow skin healing are caused after a face operation, skin is dry, rough, loose and free of elasticity, pigmentation is likely to be generated, or scars are left and hard to heal. The mask liquid with the repairing function is prepared from, by weight, 1-15 parts of butanediol, 1-15 parts of propylene glycol, 0.1-1 part of xanthan gum, 0.1-1 part of EDTA, 0.1-1 part of allantoin, 45-96 parts of water, 3-20 parts of glucan, 0.1-2 parts of snail mucus extract, 0.01-0.3 part of drawing agent, 0.01-0.4 part of decoyl hydroximic acid, 0.5-3 parts of glycerol, 0.01-0.6 part of oligopeptides-1, 0.1-3 parts of the stem of noble dendrobium and 0.01-0.5 part of sodium hyaluronate. The preparation method of the mask liquid with the repairing function is further provided. All the components act together according to the matching relationship to achieve the functions of repairing, skin whitening and face skin regeneration promoting.

The invention provides a sulfonic quantum dot/silk fibroin composite nanofiber membrane and a preparation method and application thereof. The preparation method comprises the following steps that degumming treatment is performed on natural silk, and silk fibroin is obtained; then, fibroin is dissolved in a lithium bromide water solution, and a silk fibroin sponge is obtained through dialysis, centrifugal and freeze drying; the silk fibroin sponge is dissolved into formic acid to be mixed to be uniform, then, a sulfonate graphene quantum dot water solution is ultrasonically and uniformly dispersed into a silk fibroin-formic acid solution, and a composite nanofiber membrane is prepared through electrostatic spinning equipment; mouse fibroblasts are used for performing cell viability evaluation on the composite nanometer member, and it shows that the composite nanometer membrane has the excellent biocompatibility. The prepared composite fiber membrane has the good biocompatibility and degradability, has the potential for promoting proliferation and differentiation of human bone marrow storm stem cells, and has the good biologic medical material application prospect.

The invention discloses a temperature-sensitive hepatic cell culture support material and a preparation method thereof, and relates to an intelligent tissue engineering support material and technology, in particular to a temperature-sensitive hepatic cell culture support material, a method and technology. The support material is characterized in that: the surface of the material is relatively lyophobic at the temperature of 37 DEG C and is suitable for cell adherence/spreading and cell propagation, but the surface is hydrophilic at the temperature of 32 DEG C, so cells are desorbed from the surface automatically. The preparation method of the cell culture support material comprises the following steps of: (1) surface cleaning; (2) the temperature-sensitive carboxylation modification of a TCPS surface; (3) the preparation of amido lactobionic acid (L-NH2); and (4) the saccharifying modification of the TCPS surface. Through the preparation method, the biocompatibility and cell-bound loci of the support material are enhanced, the adsorption rate of the cells on the material surface is increased, and the intelligent support material which is suitable for hepatic tissue engineering is obtained.

The embodiment of the invention discloses an autologous collagen-containing water needle and a preparation method thereof. The autologous collagen-containing water needle comprises the following components in parts by weight: 10-20 parts of autologous collagen, 10-20 parts of hyaluronic acid, 5-10 parts of biopolysaccharide glue, 5-10 parts of a sheep placenta extract, 3-8 parts of a levorotatoryC stock solution, 2-3 parts of a grape seed extract, 2-5 parts of fructan, 2-5 parts of beta-glucan, 1-5 parts of vitamin C, 2-5 parts of amino acid, 2-3 parts of an coenzyme, 2-4 parts of an anti-allergic agent, 2-5 parts of an antioxidant, 1-3 parts of a sun-screening agent and 3-8 parts of a moisturizer. The water needle is prepared from the autologous collagen extracted from human venous blood, is autologous, does not generate exclusion, contains a variety of nutrients required for cell repair and cell growth, and has relatively strong efficacies of accelerating wound healing of skin and mucosa, promoting the cell growth, promoting wound healing and strengthening the immune system.

The invention relates to a tissue engineering meniscus repair sheet and a preparation method thereof. The prepared tissue engineering meniscus repair sheet comprises an acellular meniscal thin sheet, seed cells and growth factor slow-release carriers, wherein blind micro vias are uniformly distributed on two surfaces of the acellular meniscal thin sheet, and the seed cells and the growth factor slow-release carriers are combined on two surfaces of the acellular meniscal thin sheet. The blind micro vias distributed on the acellular meniscal thin sheet increase the area of a support material, and can be used as a storage pool of the seed cells and the growth factor slow-release carriers, so that the seed cells and the growth factor slow-release carriers can be firmly attached and are not easy to fall off. Nutrition can be persistently released through the slow-release carrier by multiple growth factors, the cell amplification efficiency is improved, the cell phenotype is also maintained, cell ageing is inhibited, and fusion of the tissue engineering meniscus repair sheet and self tissue is promoted. The prepared tissue engineering meniscus repair sheet has the structure characteristic similar to the structure characteristic of natural meniscus, and is applicable to repair meniscus injure and induce regeneration of meniscus fibrous cartilage tissue.

The invention discloses a 3D model constructed in vitro by means of a serum-free culture medium as well as a construction method of the 3D model and relates to the technical field of biological materials in tissue engineering. Due to selection variety of seed cells and good in-vitro construction repeatability, industrial preparation of the 3D model can be realized. The model is an in-vitro model formed by primary corneal epithelial cells subjected to tissue isolation, limbal stem cells, immortalized corneal epithelial cells, primary oral epithelial cells or oral mucosa squamous cancer cell lines TR146 subjected to in-vitro amplification and stratified through the serum-free culture medium with an undersurface-gas-liquid surface staged culture method.

The invention discloses a hair-growing and/or white-hair-to-black-hair essence liquid containing various peptides and plant extracts. The hair-growing and/or white-hair-to-black-hair essence liquid isprepared from tripeptide-1, copper tripeptide-1, acetyl tetrapeptide-3, a cacumen platycladi extract, a sargassum pallidum extract and bisabolol. According to the hair-growing and/or white-hair-to-black-hair essence liquid, by compounding plant traditional Chinese medicine components and small-molecular peptide components, scalp blood circulation is improved, oxidation resistance and free radicalresistance are achieved, hair follicle tissues are repaired, keratin genes are activated, hair growth is promoted, and a scalp nutrient and healthy environment is maintained. The effects of activating blood, resisting inflammation, inhibiting bacteria, inhibiting sebaceous gland excessive secretion and promoting hair follicle stem cell proliferation and differentiation are effectively achieved, the effects of relieving itching, controlling oil, strengthening hair and preventing alopecia are achieved, white hair becomes black from roots, white hair can be rapidly turned into black hair, and moreover, symptoms of alopecia and dry and withered hair can be effectively eliminated. The components are natural, safe, mild, free of irritation, capable of taking effect rapidly and free of any potential hazard.

The invention discloses a face filling agent composition for injection type cosmetic plastic surgery and a preparation method of the face filling agent composition, relates to a ace filling agent composition for cosmetic plastic surgery and a preparation method of the face filling agent composition, and aims to solve the problems that conventional cross-linked hyaluronic acid is poor in cross-linking effect, a user has red and swelling inflammation, potential safety hazards can be caused, not only is a skin repairing effect not achieved, but also vascular thrombosis can be possibly caused. Theface filling agent composition for injection type cosmetic plastic surgery comprises a cross-linked hyaluronic acid composite gel, hydroxyapatite micro particles, collagen powder, cellulose ethers, an epimedium herb extract, a white peony root extract and an injection aqueous solution. The method comprises the following steps: I, weighing materials; and II, performing mixing and sterilization. The face filling agent composition for injection type cosmetic plastic surgery, which is prepared by the invention, has a use mode of being injected into dermis of a part to be filled, of a face. The invention obtains the face filling agent composition for injection type cosmetic plastic surgery.

The invention relates to a facial repair anti-inflammatory composition and application thereof. The facial repair anti-inflammatory composition comprises SIS, active peptide, arbutin, trehalose, vitamin E and allantoin, wherein the SIS is an acellular tissue matrix composite material, and the acellular tissue matrix composite material is obtained by pulverizing an acellular tissue, degrading with digestive liquid or a lysate, then mixing with a polyelectrolyte solution, freezing, drying and pulverizing. When being used for the repair and the anti-inflammation of facial skin, the facial repair anti-inflammatory composition can achieve the efficacies of effectively promoting the metabolism, the growth, the proliferation and the differentiation of facial cells, scavenging free radicals, reducing pigment accumulation, resisting the radiation, moisturizing, resisting the inflammation and the like, thereby effectively solving the skin problems such as skin dryness, skin aging, pigmentation, irritant dermatitis, daylight dermatitis, hormone dependent dermatitis and the like.

The invention relates to a method for preparing a porcine hepatocyte and mesenchymal stem cell co-microencapsulated internal bio-artificial liver. The invention comprises the following steps: establishing an in-vitro culture amplification system for porcine mesenchymal stem cells, performing in-situ perfusion separation of porcine primary hepatocytes by a two-step collagenase method, and preparing alginate-poly-L-lysine microencapsulated hepatocytes and mesenchymal stem cells. The invention overcomes the defects that internal cells usually can not survive for a long time; the proliferation ability is quite limited; and the function of the injected hepatocytes for generating various stimulating factors for promoting liver regeneration is inhibited. The invention introduces mesenchymal stem cells into an internal bio-artificial liver system, fully simulates the in-vivo microenvironment of hepatocytes by heterogeneous intercellular interaction, effectively prolongs the survival time of hepatocytes, promotes the proliferation and differentiation of hepatocytes, maintains the specific biological functions of hepatocytes, and thus significantly improves the efficacy of the internal bio-artificial liver on treating hepatic failure.

The invention belongs to the technical field of tissue engineering biomedical applications, and discloses an antibacterial piezoelectric Mxene composite material and an application thereof. The composite material is obtained by the steps of adding Mxene into a nano niobate, loading the nano niobate on the surface of the Mxene in situ to obtain an Mxene composite piezoelectric material, soaking theMxene composite piezoelectric material in a solvent containing an antibacterial drug, and performing vacuum drying at 40-80 DEG C to obtain the composite material, wherein the Mxene is M<n+1>X<n>T<x>, n = 1 to 3, X is C or N, M is a transition metal element selected from Sc, Ti, Zr, Hf, V, Nb, Ta, Cr, Mo and Mn, T<x> represents a surface functional group, and the surface functional group is -O, -OH or -F. The Mxene composite material provided by the invention has large porosity, and has the characteristics of promoting formation of calcium and phosphate salts on the surface of an implant, promoting proliferation and differentiation of bone cells and cell adhesion under the action of external pressure, and having good biocompatibility and a simple preparation method.

The invention belongs to the field of daily chemicals. A bubble hair conditioner comprises the following components in percentage by mass: 1-2% of saponin, 0.1-11% of a nonionic surfactant, 0.1-3% of a cationic surfactant, 0.1-18% of a humectant, 1-10% of proline, 0.1-1% of lactic acid, 0.1-1% of silicone oil, 0.1-5% of a plant extract and the balance of water. The hair conditioner disclosed by the invention has good film-forming property, foaming property and conditioning property, is rich and fine in foam, easy to clean, mild and low in irritation, reduces surface activity residual quantity, enables the skin to feel fresh and cool and not to be sticky and greasy, enables hair to be soft and fluffy and not to be greasy after being used, and can effectively repair hair cuticles, improve hair glossiness, smoothness and tensile strength, replenish water, preserve moisture, regulate scalp water-oil balance, and maintain the scalp environment steady state.

The invention discloses a chitosan-extracellular matrix tissue repairing membrane and a preparation method thereof and belongs to the field of biomedical materials. Extracellular matrixes are uniformly distributed in a chitosan solution, and the membrane is obtained through drying. The prepared tissue repairing membrane has excellent biocompatibility and degradability, and can promote tissue regeneration and repairing and prevent tissue adhesion.

The invention relates to the technical field of skin care products, in particular to oligopeptide repair essence and a preparation process thereof. The oligopeptide repair essence is prepared from thefollowing components in percentages by mass: 3.5-5.5% of methylpropanediol, 2-4% of glycerol, 2-4% of betaine, 1-3% of dipropylene glycol, 0.3-0.6% of bis-PEG-15 methyl ether polydimethylsiloxane, 0.2-0.5% of a glycerol glucoside mixture, 0.3-0.5% of p-hydroxyacetophenone, 0.3-0.5% of an FUCOOSORB extract, 0.1-0.3% of a Lysofix emulsifier, 0.16-0.19% of phenoxyethanol, 0.08-0.12% of dipotassium glycyrrhizinate, 0.04-0.06% of hydroxyethyl cellulose, 0.07-0.09% of Symdol 68, 0.02-0.04% of disodium EDTA (ethylene diamine tetraacetic acid), 0.01-0.03% of sodium hyaluronate, 0.01-0.03% of oligopeptide-1, 0.01-0.03% of a PENTAVITIN humectant, 0.01-0.03% of ceramide, 0.01-0.03% of a witch hazel extract and the balance of deionized water. The oligopeptide repair essence disclosed by the inventionis suitable for repairing skin, so that the skin is rapidly calmed, the damage degree of the skin after being dried in the sun is reduced, and the repair time of the skin after the skin is exposed inthe sun is shortened.

The invention discloses a method for preparing bone-like structure film layer on surface of titanium, and belongs to the technical field of biomedical materials. The method comprises the following steps of firstly utilizing three-dimensional printing laser-casting technology to do pretreatment on the surface of the metal titanium, so as to coarsen the surface of the medical metal titanium; then performing electrolytic oxidation to the medical metal titanium after being pretreated, so as to generate a titanium dioxide bone-like film layer on the surface of the medical metal titanium; and finally cleaning and drying the medical metal titanium. The film layer obtained through the method is combined strongly with the titanium base, and its surface has no microcrack and the form is uniform, these conditions are all beneficial to the adhesion of protein and nutrient of bone cells, so as to promote their differentiation and multiplication and improve the ability of bone consolidation. The method has wide application in the field of medical interplantation products such as tooth implantation.