Extract of Dioscorea sp. and the medical uses thereof
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
Problems solved by technology
Method used
Image
Examples
example 1
The Proliferative Response of Osteoprogenitor Cells of Mice Treated with the Methanol Extract and the Further Extracted Fractions of Dioscorea sp.
[0053] The cells obtained in preparatory step 4 were dispersed using a 22 G gauge needle and suspended in DMEM / F12 medium containing 15% FCS, 50 μg / ml ascorbic acid, 10 mM sodium β-glycerophosphate and 10 nM dexamethazone to form a concentration of 4.5×104 cells / ml. 225 μl cell suspension was added into each well of a 96-well microplate. After 3 hours, to the cell suspension in each well was added 25 μl methanol extract, each of the further extracted fractions and DHEA and incubated for 72 hours. Then, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed. 1 mg / ml MTT solution was added in each well and reacted for 4 hours MTT lysis buffer (20% SDS-50% DMF) was added in each well in the amount of 150 μl / well and reacted for 16 hours, and the absorbance was measured at O.D. 570 nm for the resultant cell sus...
example 2
The Effect of the Methanol Extract and Different Extracted Fractions of Dioscorea sp on the Differentiation of Matured Osteoblastic Cells of Mice Determined by Alkaline Phosphatase Activity (ALP) In Vitro
[0055] Osteoprogenitor cells collected in preparatory step 4 were incubated in a T-flask for 6 days. The cells were dispersed with 22 G gauge needle, and the cell concentration was adjusted to 5×103 cell / cm2. The cells were then incubated in 6-well plates, in which 4.5 ml of cell culture medium was added in each well, and 0.5 ml methanol extract and each of the extracted fractions were added the next day. After incubation for 14 days, alkaline phosphatase activity assay was conducted thereon, as described below.
[0056] Drawing out the culture medium, the cell layer was washed several times with PBS. 0.5% Triton X-100 in PBS was added into each well. The resultant suspension was subjected to a freezing and thawing process at a temperature of 70° C. and 37° C., respectively. The trea...
example 3
The effects of the Methanol Extract Dioscorea sp on Alkaline Phosphatase (ALP) Activity of Bone Marrow Cells Derived from a Patient Suffering from Glucocorticoid-Induced Osteoporosis
[0058] The patient's bone marrow cells obtained from the Taipei Veterans General Hospital were cultured in DMEM / F12 medium containing 15% FCS, 50 μg / ml ascorbic acid, 10 mM sodium β-glycerophosphate and 10 nM dexamethasone for 7 days. Alkaline phosphatase activity assay was conducted thereon.
[0059] As shown in FIG. 3, expression of alkaline phosphatase was noted. Compared with the control group and positive group (1 nM estrogen, which is the known treatment for osteoporosis), the methanol extract of Dioscorea sp. increased the amount of the expressed alkaline phosphatase, in which the methanol extract at 10 μg / ml showed the strongest enhancement effect.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com