3D model constructed in vitro by means of serum-free culture medium as well as construction method of 3D model

A serum-free culture, serum-free culture medium technology, applied in the field of 3D model and its construction, can solve the problems of long construction time and affect the stability of the model, achieve good repeatability, simplify the gas-liquid surface culture stage, and inhibit cell apoptosis. the effect of death
CN107326003AActive Publication Date: 2017-11-07GUANGDONG BOXI BIO TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGDONG BOXI BIO TECH CO LTD
Publication Date
2017-11-07

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Abstract

The invention discloses a 3D model constructed in vitro by means of a serum-free culture medium as well as a construction method of the 3D model and relates to the technical field of biological materials in tissue engineering. Due to selection variety of seed cells and good in-vitro construction repeatability, industrial preparation of the 3D model can be realized. The model is an in-vitro model formed by primary corneal epithelial cells subjected to tissue isolation, limbal stem cells, immortalized corneal epithelial cells, primary oral epithelial cells or oral mucosa squamous cancer cell lines TR146 subjected to in-vitro amplification and stratified through the serum-free culture medium with an undersurface-gas-liquid surface staged culture method.
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Description

Technical field

[0001] The invention belongs to the technical field of tissue engineering biomaterials, and specifically relates to a 3D model constructed in vitro using a serum-free culture system and a construction method thereof. Background technique

[0002] The 3D model in the present invention is constructed by applying the principles of cell biology and engineering to a small number of seed cells after in vitro amplification. Its structure is highly similar to that of normal human corneal epithelium. The bottom-up structure is the basal layer, The spinous layer, granular layer, and spinous layer all have no stratum corneum, have a complete epithelial structure, and express corneal-associated keratin. The 3D model constructed in vitro is mainly used to detect eye irritation in vitro to evaluate the safety of small molecule compounds, active proteins, medical devices, chemicals, cosmetics and other products.

[0003] Direct contact with chemicals or exposure to the eyes can c...

Claims

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