Temperature-sensitive hepatic cell culture support material and preparation method thereof

Abstract

The invention discloses a temperature-sensitive hepatic cell culture support material and a preparation method thereof, and relates to an intelligent tissue engineering support material and technology, in particular to a temperature-sensitive hepatic cell culture support material, a method and technology. The support material is characterized in that: the surface of the material is relatively lyophobic at the temperature of 37 DEG C and is suitable for cell adherence / spreading and cell propagation, but the surface is hydrophilic at the temperature of 32 DEG C, so cells are desorbed from the surface automatically. The preparation method of the cell culture support material comprises the following steps of: (1) surface cleaning; (2) the temperature-sensitive carboxylation modification of a TCPS surface; (3) the preparation of amido lactobionic acid (L-NH2); and (4) the saccharifying modification of the TCPS surface. Through the preparation method, the biocompatibility and cell-bound loci of the support material are enhanced, the adsorption rate of the cells on the material surface is increased, and the intelligent support material which is suitable for hepatic tissue engineering is obtained.

Description

technical field [0001] The invention relates to an intelligent tissue engineering scaffold material and technology, in particular to a temperature-sensitive liver cell culture scaffold material and method technology. Background technique [0002] my country is a high-incidence area of ​​liver disease, among which severe hepatitis / liver failure is one of the liver diseases with the most serious condition and the highest mortality rate. The three common methods of treatment for severe hepatitis / liver failure are medical treatment, liver transplantation and artificial substitute treatment. Due to the lack of liver donors and the high cost of surgery, liver transplantation is difficult to be widely used in my country, and artificial substitutes cannot completely replace all functions of human organs and cannot automatically adjust according to the requirements of the human body, so they cannot cure diseases , can only temporarily relieve symptoms or prolong the patient's life in...

AI Technical Summary

Problems solved by technology

[0006] Although many meaningful research results have been obtained on liver tissue engineering scaffold materials, according to traditional tissue engineering methods, when cells are cultured and expanded in vitro to form cell sheets or tissues, they must be transplanted into the main body together with scaffold materials, which will cause Many complicated problems are caused: (1) After the scaffold material is degraded in vivo, the space previously occupied by it will be filled with extracellular matrix, so that the cells cannot be tightly aggregated. The liver is an organ where cells are tightly aggregated, so that the transplantation The growth of the cells in the body cannot simulate the body tissue well; (2) For larger structures, around the scaffold material, the transplanted cells will grow healthily and are very similar to the body tissue, but due to passive transport, nutrients The transportation of metabolic waste and the discharge of metabolic waste are slow, resulting in the loss of cell activity in the central part of the structure, thus forming a necrotic center; (3) the acid-base of some scaffold materials, such as polylactic acid (PLA), chitin, etc. It has certain toxicity to cells; (4) Some scaffold materials, such as polylactic acid (PLA), polyglycolic alcohol (PGA), etc., degrade into small molecular fragments, which will cause the aggregation of macrophages and neutrophils in the body , these immune cells also phagocytize implanted cells

However, grafting isopropylacrylamide (NIPAAm) to the surface of polystyrene by ultraviolet light fixation method, and used for tissue engineering, has hardly been reported.