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A bio-ink, water-soluble technology, applied in medical science, prosthesis, additive processing, etc., can solve problems such as toxicity and cell damage, achieve mild reaction, promote proliferation and differentiation, and good biocompatibility.
Inactive Publication Date: 2020-02-18
RENMIN UNIVERSITY OF CHINA
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However, the cross-linking agents currently used in the gelatin cross-linking process, such as glutaraldehyde, have certain toxicity, and ultraviolet light will also cause certain damage to cells.
Method used
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Embodiment 1
[0042] Embodiment 1 Preparation of bioprinting ink
[0043] (1) The raw materials used in the bio-ink in this example consist of the following, in terms of mass percentage: 8.0% gelatin, 0.1% bacterial cellulose, 0.8% growth factor, 16.7% transglutaminase, and 74.4% sterile PBS solution.
[0044] (2) The preparation steps are as follows:
[0045] 1) Add gelatin, bacterial cellulose, and growth factors to a square petri dish in sequence at 37°C, and irradiate with ultraviolet light for 12-24 hours.
[0046] 2) Add the irradiated gelatin, bacterial cellulose, cell growth factor and sterile PBS solution in 1) into the Shu Niu bottle, and then heat it with magnetic stirring at 30-35°C for 1 hour to dissolve all the components and form a mixture (hydrogel solution).
[0047] 3) Adding counted biological cells into the hydrogel solution to make a suspension.
[0048] 4) Add transglutaminase solution to the suspension.
Embodiment 2
[0049] Embodiment 2 Preparation of bioprinting ink
[0050] (1) The raw materials used in the bio-ink in this example consist of the following components in terms of mass percentage: 8.0% gelatin, 0.2% bacterial cellulose, 0.8% growth factor, 16.7% transglutaminase, and 74.3% sterile PBS solution.
[0051] (2) The preparation method is the same as in Example 1 of the present invention to form a bioink.
Embodiment 3
[0052] Example 3 Preparation of bioprinting ink
[0053] (1) The raw materials used in the bio-ink in this example consist of the following components by mass percentage: 8.0% gelatin, 0.3% bacterial cellulose, 0.8% growth factor, 16.7% transglutaminase, and 74.2% sterile PBS solution.
[0054] (2) The preparation method is the same as in Example 1 of the present invention to form a bioprinting ink.
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Abstract
The invention discloses a biological ink used for 3D biological printing and a preparation method thereof. The biological ink comprises the following components in percentage by mass: 8%-20% of a hydrophilic polymer with the crosslinking function, 10-20% of a water-soluble enzyme with the crosslinking function, 0.1%-1% of bacterial cellulose, and the balance a sterile PBS solution. The biologicalink can be used for 3D printing after mixed with cells, and the shear thinning properties of the used materials make the biological ink have the characteristics of self-supporting after extrusion. Theused materials have good cell compatibility, the enzyme crosslinking agent forms a hydrogel at physiological temperature (about 37 DEG C), the reaction conditions are mild, the cells are not damaged,a hydrogel support has relatively good stability in a culture medium, and thereby cell proliferation can be supported.
Description
technical field [0001] The invention belongs to the technical field of bio-ink preparation, and in particular relates to a bio-ink with excellent biocompatibility, a preparation method and application thereof. Background technique [0002] 3D bioprinting is a technology based on the principle of discrete-stacking forming, using living cells, bioactive factors and basic forming units of biomaterials to design and manufacture biologically active artificial organs, implants or three-dimensional cell structures. It combines manufacturing science and Biomedicine is an emerging technology with intersecting and cutting-edge features. 3D bioprinting has the characteristics of high precision, high efficiency, and personalized manufacturing. It has attracted enough attention in the field of biomedicine and has a very broad application prospect. [0003] Bioinks are materials that can be used for 3D bioprinting, usually fluids containing nutrients, matrix components and cells. An ide...
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