Biological ink and preparation method thereof
A bio-ink, water-soluble technology, applied in medical science, prosthesis, additive processing, etc., can solve problems such as toxicity and cell damage, achieve mild reaction, promote proliferation and differentiation, and good biocompatibility.
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Embodiment 1
[0042] Embodiment 1 Preparation of bioprinting ink
[0043] (1) The raw materials used in the bio-ink in this example consist of the following, in terms of mass percentage: 8.0% gelatin, 0.1% bacterial cellulose, 0.8% growth factor, 16.7% transglutaminase, and 74.4% sterile PBS solution.
[0044] (2) The preparation steps are as follows:
[0045] 1) Add gelatin, bacterial cellulose, and growth factors to a square petri dish in sequence at 37°C, and irradiate with ultraviolet light for 12-24 hours.
[0046] 2) Add the irradiated gelatin, bacterial cellulose, cell growth factor and sterile PBS solution in 1) into the Shu Niu bottle, and then heat it with magnetic stirring at 30-35°C for 1 hour to dissolve all the components and form a mixture (hydrogel solution).
[0047] 3) Adding counted biological cells into the hydrogel solution to make a suspension.
[0048] 4) Add transglutaminase solution to the suspension.
Embodiment 2
[0049] Embodiment 2 Preparation of bioprinting ink
[0050] (1) The raw materials used in the bio-ink in this example consist of the following components in terms of mass percentage: 8.0% gelatin, 0.2% bacterial cellulose, 0.8% growth factor, 16.7% transglutaminase, and 74.3% sterile PBS solution.
[0051] (2) The preparation method is the same as in Example 1 of the present invention to form a bioink.
Embodiment 3
[0052] Example 3 Preparation of bioprinting ink
[0053] (1) The raw materials used in the bio-ink in this example consist of the following components by mass percentage: 8.0% gelatin, 0.3% bacterial cellulose, 0.8% growth factor, 16.7% transglutaminase, and 74.2% sterile PBS solution.
[0054] (2) The preparation method is the same as in Example 1 of the present invention to form a bioprinting ink.
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