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Sulfonic quantum dot/silk fibroin composite nanofiber membrane and preparation method and application thereof

A technology for composite nanofiber and silk fibroin, applied in the field of sulfonic acid quantum dot/silk fibroin composite nanofiber membrane and its preparation

Pending Publication Date: 2019-11-15
SHANGHAI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, single-component silk fibroin electrospun fiber membranes have been reported, but the preparation of composite fiber membranes by electrospinning by blending edge sulfonated quantum dot nanomaterials with silk fibroin has not been reported yet.

Method used

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  • Sulfonic quantum dot/silk fibroin composite nanofiber membrane and preparation method and application thereof
  • Sulfonic quantum dot/silk fibroin composite nanofiber membrane and preparation method and application thereof
  • Sulfonic quantum dot/silk fibroin composite nanofiber membrane and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The present embodiment is the preparation method of a preferred sulfonic acid-based quantum dots / silk fibroin composite nanofiber membrane, which comprises the following steps: 0.5% sodium carbonate (sodium carbonate ( Na 2 CO 3 ) aqueous solution, boiled for 30 minutes, picked up, rinsed with a large amount of deionized water, repeated the above steps three times to carry out degumming treatment, air-dried the silk at room temperature, loosened and packaged for later use. Take 2g of degummed silk and dissolve it in 9mol / L lithium bromide (LiBr) aqueous solution at 40°C for 2 hours, place the resulting solution in a constant temperature laboratory for 10 hours, inject it into a dialysis bag with a molecular weight cut-off of 7000, and dialyze it with deionized water. Stir continuously at low temperature, change the deionized water every 2 hours, centrifuge the obtained solution at a speed of 10000r / min for 15min to remove impurities after 3 days, and vacuum freeze-dry ...

Embodiment 2

[0053] The present embodiment is the preparation method of a preferred sulfonic acid-based quantum dot / silk fibroin composite nanofiber membrane, which comprises the following steps: putting 20 g of shredded cocoons into 1000 ml of boiling mass concentration of 0.7% sodium carbonate ( Na 2 CO 3 ) aqueous solution, boiled for 20 minutes, picked up, rinsed with a large amount of deionized water, repeated the above steps three times to carry out degumming treatment, air-dried the silk at room temperature, loosened and packaged for later use. Take 2g of degummed silk and dissolve it in 6mol / L lithium bromide (LiBr) aqueous solution at 40°C for 3 hours, place the resulting solution in a constant temperature laboratory for 12 hours, inject it into a dialysis bag with a molecular weight cut-off of 8000, and dialyze it with deionized water. Stir continuously at low temperature, change the deionized water every 1.5 hours, centrifuge the obtained solution at a speed of 12000r / min for 1...

Embodiment 3

[0055] This embodiment is the cytotoxicity experiment of the sulfonic acid-based quantum dots / silk fibroin composite nanofiber membrane prepared in Example 1:

[0056] The biocompatibility of the fiber membrane prepared in Example 1 was evaluated according to the ISO10993-5 standard test (indirect contact) method. After the composite nanofibrous membrane was soaked in 75% and sterilized by ultraviolet light, it was immersed in a 24-well polystyrene plate containing complete medium at 37°C (6 cm 2 ·mL -1 ) for 24 hours. Mouse fibroblasts (L929) were used at 5×10 per well 3 The density was seeded in a 96-well plate in 5% CO 2 After 24 hours of incubation in a 37°C incubator, replace the medium in the 96-well plate with 100 μL of the extraction medium in the 24-well plate and continue to cultivate for 24 hours, then add 10 μL of CCK8 reagent to each well, and continue to culture in the incubator After 4 hours, the absorbance at 450 nm was measured with a microplate reader. T...

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Abstract

The invention provides a sulfonic quantum dot / silk fibroin composite nanofiber membrane and a preparation method and application thereof. The preparation method comprises the following steps that degumming treatment is performed on natural silk, and silk fibroin is obtained; then, fibroin is dissolved in a lithium bromide water solution, and a silk fibroin sponge is obtained through dialysis, centrifugal and freeze drying; the silk fibroin sponge is dissolved into formic acid to be mixed to be uniform, then, a sulfonate graphene quantum dot water solution is ultrasonically and uniformly dispersed into a silk fibroin-formic acid solution, and a composite nanofiber membrane is prepared through electrostatic spinning equipment; mouse fibroblasts are used for performing cell viability evaluation on the composite nanometer member, and it shows that the composite nanometer membrane has the excellent biocompatibility. The prepared composite fiber membrane has the good biocompatibility and degradability, has the potential for promoting proliferation and differentiation of human bone marrow storm stem cells, and has the good biologic medical material application prospect.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a sulfonic acid-based quantum dot / silk fibroin composite nanofiber membrane and a preparation method and application thereof. Background technique [0002] Mulberry silk is mainly composed of sericin and silk fibroin, of which sericin accounts for about 25% and silk fibroin accounts for about 70%. Sericin has good water solubility, while silk fibroin is insoluble in water, so the sericin protein can be removed by simple alkali or enzyme degumming, and the silk fibroin protein with excellent plasticity we need can be obtained. Silk fibroin is a natural biopolymer with good biocompatibility and excellent biodegradability, so it has attracted extensive attention in the field of biomaterials. In order to meet the requirements of various applications, silk fibroin needs It can be dissolved into various forms such as gel, powder, film, and electrospun fiber. [0003] Ele...

Claims

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Application Information

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IPC IPC(8): D04H1/728D01D5/00D01C3/02D01F4/02
CPCD04H1/728D01D5/003D01D5/0061D01C3/02D01F4/02D10B2509/00
Inventor 施文彦季舒婷徐沁王云沈龙祥吴明红徐刚唐量
Owner SHANGHAI UNIV
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