14 results about "Mouse Fibroblast" patented technology

The invention discloses a monoclonal antibody targeting all CD45 subtypes and application of the monoclonal antibody, and belongs to the field of monoclonal antibody preparation. The hybridoma cell strain capable of stably secreting all anti-human CD45 subtype antibodies is successfully obtained by stably expressing short and long molecular subtypes of human CD45 extracellular regions in L929 mouse fibroblasts, immunizing BALB / c mice with L929 cells expressing the two molecular subtypes of human CD45, and fusing splenocytes with SP20 myeloma cells after the serum titer reaches the standard. And the monoclonal antibody targeting all subtypes of CD45 is obtained. The monoclonal antibody provided by the invention shows a high-affinity binding characteristic with all human CD45 molecular subtypes, has important application value in the aspect of human CD45 molecular detection, and can be used as a therapeutic antibody in the fields of tumor immunotherapy, autoimmune disease treatment, transplant rejection resistance and the like.

The invention belongs to the technical field of biological medicines, and particularly relates to an antibacterial peptide CeP28 from red deer as well as a preparation method and application thereof. The amino acid sequence of the antibacterial peptide CeP28 is shown as SEQ ID NO.1, and the antibacterial peptide CeP28 has good broad-spectrum antibacterial activity and has good antibacterial activity on gram-negative bacteria, gram-positive bacteria and fungi. Meanwhile, the antibacterial peptide CeP28 has low hemolysis activity on sheep red blood cells and low toxicity on mouse fibroblasts L929. Therefore, the antibacterial peptide CeP28 disclosed by the invention can be used as a potential broad-spectrum anti-infection drug.

The invention belongs to the technical field of biology, and discloses a serum-free culture medium and application thereof in obtaining nerve cells through embryo fibroblast transdifferentiation. The serum-free culture medium consists of a basic culture medium, a protein additive and cell culture auxiliary components, the protein additive consists of transferrin, a leukemia inhibition factor and a basic fibroblast growth factor; the final concentration of the transferrin is 5 ng / mL-30 ng / mL, the final concentration of the leukemia inhibition factor is 50 ng / mL-150 ng / mL, and the final concentration of the basic fibroblast growth factor is 10 ng / mL-25 ng / mL. The serum-free culture medium can promote the conversion speed of transdifferentiation of fibroblasts into nerve cells, can significantly improve the transdifferentiation efficiency, and can improve the cell survival rate of transdifferentiation of mouse embryo fibroblasts into nerve cells.

The invention discloses recombinant collagen with a triple helix structure as well as a preparation method and application of the recombinant collagen, and belongs to the technical field of biochemical engineering. The recombinant collagen with the triple helix structure provided by the invention comprises at least one of CAIII81C, CAIII142C and CAIII405C, and the recombinant collagen with the triple helix structure comprises at least one of CAIII81C, CAIII142C and CAIII405C, the amino acid sequence of the CAIII81C is as shown in SEQ ID NO: 1, the amino acid sequence of the CAIII142C is as shown in SEQ ID NO: 2, and the amino acid sequence of the CAIII405C is as shown in SEQ ID NO: 3. The eukaryotic expression system realizes the expression of the recombinant collagen CAIII81C, CAIII142C and CAIII405C with the triple helix structure, and the triple helix structure stably exists at the temperature of 65 DEG C. An activity experiment result of mouse embryo fibroblast (BALB / c 3T3) migration shows that the recombinant collagen CAIII81C, the recombinant collagen CAIII142C and the recombinant collagen CAIII405C expressed by the invention have very good activity of promoting cell migration.

The invention provides activity evaluation and application of recombinant humanized III-type collagen, which comprises the following steps of: carrying out cell proliferation, cell adhesion and cell migration experiments on the recombinant humanized III-type collagen by adopting a plurality of cell models, namely human skin fibroblasts HSF, cuticle cells HaCat and mouse fibroblasts 3T3; the bioactivity of the recombinant humanized III-type collagen is subjected to comprehensive and deep analysis from multiple dimensions, so that multi-cell and multi-aspect accurate evaluation is realized, and the biological characteristics and functional expressions of the collagen in different cell environments are accurately captured. Experimental data can provide a powerful experimental basis for the application of the recombinant humanized III-type collagen in the fields of cosmetics, medical instruments and the like, so that the potentials of the recombinant humanized III-type collagen in the aspects of cosmetic effects of improving skin appearance, promoting skin repair and the like are clear; and a key reference can be provided for the properties such as tissue repair and cell adhesion involved in the development of medical apparatuses and instruments.

The present invention relates to a composition for preventing, alleviating, or treating respiratory diseases, the composition comprising Codonopsis lanceolata, Saururus chinensis, Platycodon grandiflorum, and propolis as active ingredients. The composition according to the present invention was proven to be effective in preventing, alleviating or, treating respiratory diseases on the basis of experiments using a mouse fibroblast cell model.

The present invention discloses a reagent composition for identifying mouse fibroblast subpopulations and a method for using the same, relating to the field of cell technology. The reagent composition includes an antigen blocking solution, an allophycocyanin (APC)-labeled Ly6C antibody, a phycoerythrin (PE)-labeled CyclinD1 antibody, a fluorescein isothiocyanate (FITC)-labeled DCN antibody, a PBS phosphate buffer, a cell digestion solution, and an erythrocyte lysis solution. The present invention can identify and sort DCN-positive fibroblast subpopulations in the lung tissue of mice with acute lung injury, or identify and sort DCN-positive fibroblast subpopulations in the lung tissue of mice with acute lung injury that are undergoing tissue damage or repair, providing conditions for in-depth research on the role and mechanism of DCN-positive fibroblasts in ARDS, and exploring clinical treatment or prognostic targets.

The present invention relates to the field of biotechnology, specifically to a tuna scale oligopeptide with the efficacy of repairing skin ultraviolet damage, its preparation method and application. The present invention uses tuna scales as raw materials, and through pretreatment, composite enzymatic hydrolysis, membrane ultrafiltration, and chromatographic separation and purification, an oligopeptide Tyr-Ser-Gly-Pro-Leu-Gly-Ile-Arg (YSGPLGIR) with the efficacy of repairing skin ultraviolet damage is obtained. The molecular weight measured by ESI-MS is 862.0 Da. The oligopeptide YSGPLGIR prepared by the present invention with the efficacy of repairing skin ultraviolet damage can significantly improve the survival rate and antioxidant enzyme activity of ultraviolet-damaged mouse fibroblasts (NIH 3T3), and can be applied to the preparation of drugs and health products related to the treatment of skin ultraviolet damage.

The application discloses a kind of circular RNA of expressing collagen type III polypeptide and application thereof, belong to collagen preparation technical field.The application provides a kind of circular recombinant RNA by no-scar design, element screening, with high translation efficiency, good cyclization effect, strong expression stability, more durable, more close to nature, nucleotide chain is sequentially connected by promoter, 5'homologous arm, 3'intron, IRES sequence, target protein coding sequence, 5'intron, 3'homologous arm, nucleotide sequence is as shown in SEQ ID NO.8;Circular recombinant RNA can successfully express collagen type III peptide in ex vivo human fibroblast, mouse fibroblast and mouse in vivo, to a certain extent, reverse ultraviolet light-induced photoaging process, improve skin photoaging damage and promote tissue repair, with high cell uptake rate, strong expression stability, good persistence;Low immunogenicity, good biocompatibility and in vitro safety.

The application belongs to the technical field of cell engineering, and particularly relates to a mammalian target of rapamycin (mTOR) self-activation mouse model and a construction method and application of a mouse embryonic fibroblast cell line. In the application, a serine (S) at position 2215 of an mTOR protein is mutated into a tyrosine (Y), and a transgenic mouse model of an mTORS2215Y activation mutation, i.e. a mammalian target of rapamycin (mTOR) self-activation mouse model, is constructed. The transgenic mouse model is used to construct a mouse embryonic fibroblast cell line which can be immortalized, so that the related mechanism research of the mTOR self-activation mutation can be carried out at a cell level, and the screening of related therapeutic drugs can be carried out.

The application discloses a kind of Wang Baihe glycosides compound and application thereof, wherein Wang Baihe glycosides compound is any one of compound I, compound II, compound III, which is a new chemical component found in lily.The application carries out structural identification to the compound separated by the above method by chromatography, modern spectroscopy and the like means.The application also carries out cell proliferation experiment using mouse embryonic fibroblast NIH-3T3, and finds that the compound has the function of promoting skin cell proliferation, and can be used in skin protection and repair field.

The invention discloses a cell membrane modified PLGA porous microsphere and a preparation method and application thereof.The preparation method comprises the steps that a cell membrane and a PLGA porous microsphere aqueous solution are evenly mixed and then subjected to water bath ultrasonic treatment for 20-40 s, then stirring is conducted for 20-40 min, freeze drying is conducted after collection, and the cell membrane modified PLGA porous microsphere is obtained, wherein the cell membrane is obtained by extracting mouse fibroblasts (L929) or human umbilical vein endothelial cells (HUVEC) as homologous cells. According to the prepared cell membrane modified PLGA porous microsphere, the through pore structure and uniform pore size distribution of the PLGA porous microsphere are kept, the affinity and adhesion of the cell membrane modified PLGA porous microsphere to homologous cells are enhanced, and meanwhile, the cell membrane modified PLGA porous microsphere is used for homologous cell delivery to improve the loading efficiency, survival rate and proliferation activity of loaded homologous cells.

The invention belongs to the technical field of biology, and particularly discloses application of dihydromyricetin in preparation of an inflammatory factor inhibitor for regulating immune homeostasis, the inhibitor comprises a medicine for reducing the content of inflammatory factors in cells and / or the content of cyclin, and specifically comprises dihydromyricetin; the inflammatory factors comprise Il8, Il1-alpha, Il1-beta and Il6. According to the application, by establishing a H2O2 induced human skin fibroblast premature senility model and a H2O2 induced mouse fibroblast premature senility model, the dihydromyricetin is found to be used as an inhibitor, so that overexpression of inflammatory factors including Il8, Il1-alpha, Il1-beta and Il6 can be effectively reduced, expression of cyclin P16 is also reduced, and the inflammatory factors such as inflammatory factors including Il8, Il1-alpha, Il1-beta and Il6 can be effectively inhibited. The anti-aging and anti-inflammatory effects are effectively achieved in mammals, and the immune homeostasis is maintained.

The invention belongs to the technical field of biomedical materials and oral implantation, and particularly relates to a titanium alloy oral implant with a gradient porous structure and a partitioned modified surface and a preparation method of the titanium alloy oral implant. The implant is totally divided into an upper area and a lower area, the lower area adopts an innovative three-stage gradient porous structure, the neck edge area is provided with small holes, the middle transition area is provided with middle holes, and the root area is provided with large holes; after the implant is embedded into a jaw bone part, the elasticity modulus is gradually reduced from the crown part to the root part, the implant is matched with the mechanical property of a natural alveolar bone, and the stress shielding effect is effectively reduced. The surface of the implant is subjected to partitioned modification treatment through a femtosecond laser technology, and adhesion and proliferation of mouse fibroblasts are remarkably promoted after the upper region is treated; a specific micro-nano composite structure is formed after the lower region is treated, so that the proliferation rate of osteoblasts and the activity of alkaline phosphatase can be remarkably improved. Through the structure-function integrated design, mechanical suitability optimization, soft tissue closure enhancement and bone combination efficiency improvement are achieved at the same time.