6 results about "Mouse Fibroblast" patented technology

The invention belongs to the technical field of biological medicines, and particularly relates to an antibacterial peptide CeP28 from red deer as well as a preparation method and application thereof. The amino acid sequence of the antibacterial peptide CeP28 is shown as SEQ ID NO.1, and the antibacterial peptide CeP28 has good broad-spectrum antibacterial activity and has good antibacterial activity on gram-negative bacteria, gram-positive bacteria and fungi. Meanwhile, the antibacterial peptide CeP28 has low hemolysis activity on sheep red blood cells and low toxicity on mouse fibroblasts L929. Therefore, the antibacterial peptide CeP28 disclosed by the invention can be used as a potential broad-spectrum anti-infection drug.

The invention belongs to the technical field of biology, and discloses a serum-free culture medium and application thereof in obtaining nerve cells through embryo fibroblast transdifferentiation. The serum-free culture medium consists of a basic culture medium, a protein additive and cell culture auxiliary components, the protein additive consists of transferrin, a leukemia inhibition factor and a basic fibroblast growth factor; the final concentration of the transferrin is 5 ng / mL-30 ng / mL, the final concentration of the leukemia inhibition factor is 50 ng / mL-150 ng / mL, and the final concentration of the basic fibroblast growth factor is 10 ng / mL-25 ng / mL. The serum-free culture medium can promote the conversion speed of transdifferentiation of fibroblasts into nerve cells, can significantly improve the transdifferentiation efficiency, and can improve the cell survival rate of transdifferentiation of mouse embryo fibroblasts into nerve cells.

The present invention relates to a composition for preventing, alleviating, or treating respiratory diseases, the composition comprising Codonopsis lanceolata, Saururus chinensis, Platycodon grandiflorum, and propolis as active ingredients. The composition according to the present invention was proven to be effective in preventing, alleviating or, treating respiratory diseases on the basis of experiments using a mouse fibroblast cell model.

The application discloses a kind of circular RNA of expressing collagen type III polypeptide and application thereof, belong to collagen preparation technical field.The application provides a kind of circular recombinant RNA by no-scar design, element screening, with high translation efficiency, good cyclization effect, strong expression stability, more durable, more close to nature, nucleotide chain is sequentially connected by promoter, 5'homologous arm, 3'intron, IRES sequence, target protein coding sequence, 5'intron, 3'homologous arm, nucleotide sequence is as shown in SEQ ID NO.8;Circular recombinant RNA can successfully express collagen type III peptide in ex vivo human fibroblast, mouse fibroblast and mouse in vivo, to a certain extent, reverse ultraviolet light-induced photoaging process, improve skin photoaging damage and promote tissue repair, with high cell uptake rate, strong expression stability, good persistence;Low immunogenicity, good biocompatibility and in vitro safety.

The application discloses a kind of Wang Baihe glycosides compound and application thereof, wherein Wang Baihe glycosides compound is any one of compound I, compound II, compound III, which is a new chemical component found in lily.The application carries out structural identification to the compound separated by the above method by chromatography, modern spectroscopy and the like means.The application also carries out cell proliferation experiment using mouse embryonic fibroblast NIH-3T3, and finds that the compound has the function of promoting skin cell proliferation, and can be used in skin protection and repair field.

The invention discloses a cell membrane modified PLGA porous microsphere and a preparation method and application thereof.The preparation method comprises the steps that a cell membrane and a PLGA porous microsphere aqueous solution are evenly mixed and then subjected to water bath ultrasonic treatment for 20-40 s, then stirring is conducted for 20-40 min, freeze drying is conducted after collection, and the cell membrane modified PLGA porous microsphere is obtained, wherein the cell membrane is obtained by extracting mouse fibroblasts (L929) or human umbilical vein endothelial cells (HUVEC) as homologous cells. According to the prepared cell membrane modified PLGA porous microsphere, the through pore structure and uniform pore size distribution of the PLGA porous microsphere are kept, the affinity and adhesion of the cell membrane modified PLGA porous microsphere to homologous cells are enhanced, and meanwhile, the cell membrane modified PLGA porous microsphere is used for homologous cell delivery to improve the loading efficiency, survival rate and proliferation activity of loaded homologous cells.