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Mouse Embryonic Fibroblasts (MEFs) are a type of fibroblast prepared from mouse embryo. MEFs show a spindle shape when cultured in vitro, a typical feature of fibroblasts. The MEF is a limited cell line.
Circular RNA expressing collagen type III polypeptide and application thereof
The application discloses a kind of circular RNA of expressing collagen type III polypeptide and application thereof, belong to collagen preparation technical field.The application provides a kind of circular recombinant RNA by no-scar design, element screening, with high translation efficiency, good cyclization effect, strong expression stability, more durable, more close to nature, nucleotide chain is sequentially connected by promoter, 5'homologous arm, 3'intron, IRES sequence, target protein coding sequence, 5'intron, 3'homologous arm, nucleotide sequence is as shown in SEQ ID NO.8;Circular recombinant RNA can successfully express collagen type III peptide in ex vivo human fibroblast, mouse fibroblast and mouse in vivo, to a certain extent, reverse ultraviolet light-induced photoaging process, improve skin photoaging damage and promote tissue repair, with high cell uptake rate, strong expression stability, good persistence;Low immunogenicity, good biocompatibility and in vitro safety.
Owner:DALIAN LINLIN BIOTECHNOLOGY CO LTD