Method for culturing oral mucosa cells

A technology of oral mucosal cells and culture methods, applied in the field of preparation of urethral stents, which can solve the problems of inability to achieve tissue engineering, weakened cell adhesion and proliferation, and prone to aging of cells

Inactive Publication Date: 2015-08-26
SHANGHAI TONGJI HOSPITAL
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0003] The invention provides a method for culturing oral mucosa cells, which solves the technical problem that in the prior art oral mucosa culture met

Method used

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  • Method for culturing oral mucosa cells

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Embodiment Construction

[0014] In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0015] Embodiments of the present invention provide a method for culturing oral mucosal cells, such as figure 1 shown, including:

[0016] Step 101, using digestive enzymes to separate the rabbit oral mucosa epithelium from the connective tissue;

[0017] Wherein, the digestive enzyme is Dispase II digestive enzyme. Dispase II digestive enzyme is a rapid, effective, and mild neutral protease extracted from the culture medium of Bacillus polymyxa, which can selectively separate fibronectin and type I collagen on the basement membrane, thereby separating Epithelium and dermis, but no effect on epithelial cells and cell junctions. After digestion with Dispase II digestive enzyme, the proliferative cells located in the basal layer ...

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Abstract

The invention relates to the field of preparation of urethral stents and discloses a method for culturing oral mucosa cells. The method comprises the following steps: separating oral mucosal epithelium and connective tissues of a rat by using digestive enzymes, and digesting by using pancreatin so as to remove the connective tissues, thereby obtaining the oral mucosa cells; inoculating the oral mucosa cells to mouse fibroblast cells 3T3 which are inactivated by mitomycin, and mixing keratinocyte serum-free culture solution KSFM and DMEM to form the mixed solution; and culturing the oral mucosa cells inoculated to the cells 3T3 by adopting the mixed solution. According to the method disclosed by the invention, the separation purity of the oral mucosa cells is improved, and the adherence and proliferation capacities of seed cells can be improved.

Description

technical field [0001] The invention relates to the field of preparation of urethral stents, in particular to a method for culturing oral mucosal cells. Background technique [0002] The in vitro culture of oral mucosal epithelial cells has important biological and clinical significance. It not only provides a good experimental system for the study of oral mucosal differentiation, oral pathophysiology, toxicology and carcinogenesis, but also has great significance for the construction of human tissue engineering oral mucosa and the repair of oral mucosal tissue defects. The repair and reconstruction of mucosal defects such as trachea and urethra have important clinical significance. The construction of tissue-engineered urethra requires a sufficient number of seed cells. People try to use oral mucosa cells as seed cells, and the proliferation ability and purity of seed cells are closely related to the effect of cell separation. In the oral mucosa culture method in the prior...

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Application Information

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IPC IPC(8): C12N5/071
Inventor 李超吴登龙徐月敏乐威
Owner SHANGHAI TONGJI HOSPITAL
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