Method for culturing oral mucosa cells

Abstract

The invention relates to the field of preparation of urethral stents and discloses a method for culturing oral mucosa cells. The method comprises the following steps: separating oral mucosal epithelium and connective tissues of a rat by using digestive enzymes, and digesting by using pancreatin so as to remove the connective tissues, thereby obtaining the oral mucosa cells; inoculating the oral mucosa cells to mouse fibroblast cells 3T3 which are inactivated by mitomycin, and mixing keratinocyte serum-free culture solution KSFM and DMEM to form the mixed solution; and culturing the oral mucosa cells inoculated to the cells 3T3 by adopting the mixed solution. According to the method disclosed by the invention, the separation purity of the oral mucosa cells is improved, and the adherence and proliferation capacities of seed cells can be improved.

Description

technical field

[0001] The invention relates to the field of preparation of urethral stents, in particular to a method for culturing oral mucosal cells. Background technique

[0002] The in vitro culture of oral mucosal epithelial cells has important biological and clinical significance. It not only provides a good experimental system for the study of oral mucosal differentiation, oral pathophysiology, toxicology and carcinogenesis, but also has great significance for the construction of human tissue engineering oral mucosa and the repair of oral mucosal tissue defects. The repair and reconstruction of mucosal defects such as trachea and urethra have important clinical significance. The construction of tissue-engineered urethra requires a sufficient number of seed cells. People try to use oral mucosa cells as seed cells, and the proliferation ability and purity of seed cells are closely related to the effect of cell separation. In the oral mucosa culture method in the prior...

Images