86results about How to "Improve the quality of healing" patented technology

The invention discloses a double-layer membranous tissue repair material and a preparation method thereof, wherein, a cell-free membranous biological derivative material is used as a surface layer, and a fibroblast is compounded in the interior of a biological support material to form a substrate, and then the surface layer and the substrate are combined in a chimeric way to form the double-layer membranous tissue repair material; a compact surface layer structure can effectively reduce the loss of water, electrolytes and protein from surface of wound, avoid the invading and the reproduction of bacteria to the impaired surface of wound as well as prevent the infection of the surface of wound, thus being beneficial to epitheliosis and epithelial growth; the substrate can directly repair the surface of wound, promote the ingrowth of cells around the surface of wound and the angiogenesis, induce the differentiation from stem cells to skin cells and quicken wound healing; compared with the existing products, the tissue repair material has the advantages of being capable of promoting the regeneration of skin, improving the elasticity, the flexibility and the mechanical abrasion resistance of skin after the surface of wound is healed, reducing hyperplasia of scar tissues, controlling the contracture, having excellent biocompatibility, increasing the success rate of transplant and improving the quality of healing; the invention has wide material resources and simple production method; the double-layer membranous tissue repair material prepared is applicable to the clinical treatment of skin defect caused by inflammation, ulcer, thermal burns, iatrogenicity and the like.

The present invention relates to a composition for reducing sport injury and promoting sport injury repair. The composition comprises the following raw materials and auxiliary materials, by weight, 500-2000 parts of hydrolyzed collagen protein, 100-400 parts of soybean protein peptide, 50-400 parts of chondroitin sulphate, 50-400 parts of glucosamine or a salt thereof, 100-1000 parts of a traditional Chinese medicine, 10-400 parts of haematococcus pluvialis, 1-100 parts of vitamin, and 1-100 parts of a mineral. The composition provides good effects for sport injury reduction and sport injury repair promotion.

Disclosed is a cell sheet for tissue repair and bio-artificial tissue engineering. The cell sheet comprises treated stem cell embedded in its self-secreted extracellular matrix (ECM) and formed a cell sheet. The cell sheet is formed by isolating the stem cell, expanding the stem cell and treating the stem cell with biological factors or factors leading to the production of biological factors, to induce its differentiation, production of extracellular matrix and formation of a cell sheet in vitro. The cell sheet is used as a bioactive material or as an acellular material for the promotion of tissue repairs or used to form a bio-artificial organ for tissue replacement. The cell sheet of the present invention eliminates the need to use scaffolds for cell delivery. The cell sheet facilitates in vivo cell transplantation and provides some tensile mechanical strength for bearing early mechanical load during tissue repair.

The invention relates to a double-layer collagen dermal scaffold and a preparation method thereof; the double-layer collagen dermal scaffold comprises a collagen layer and a silicone layer; the collagen layer is a collagen sponge layer, and the silicone layer is a silicone mixture layer or a pretreated silicone mixture layer; the collagen layer induces cell migration and promotes proliferation and differentiation so as to accelerate the recovery of an affected part; along with the degrading of material, regenerated tissues integrated to tissues around are finally formed; the silicone layer is semipermeable membrane, which is capable of controlling water loss of the affected part, protecting the wound and avoiding infection, has good mechanical strength and helps suture and fix the double-layer collagen dermal scaffold; by combining collagen and the silicone layer, it is possible to accelerate the growth of granulation tissues; by controlling collagen concentration and pH value to control the degrading rate of the collagen layer, the silicone layer can be removed smoothly after the granulation tissue phase; by transplanting auto-epidermis, healing cycle can be shortened, healing quality can be improved, and scars are decreased.

The invention discloses a fusion protein with antibacterial and repairing function and a production method and application thereof. A fusion gene is prepared by splicing an anthropogenic antibacterial peptide gene and a cell growth factor gene through a segment of hydrophobic polypeptide joint; and the fusion protein is prepared by adopting the expression of a prokaryocyte or yeast expression system, and is encoded by the anthropogenic antibacterial peptide, the polypeptide joint and the cell growth factor gene from an N end to a C end of the fusion protein. The invention simultaneously provides application of the fusion protein to the preparation of medicaments for treating wounds, burns/scalds, cold injury, chronic ulcer or bedsore. The invention creatively fuses the two kinds of polypeptide genes together to successfully construct a fusion protein with dual function of preventing infection and accelerating the repair of the damaged tissue, improves the safety of clinical medicament simultaneously, and prolongs the half-life period of the fusion protein.

The invention belongs to the technical field of plant biology, in particular relates to a method of tissue culture of Medicago sativa L. By way of developing a novel tissue culture technical method, the invention focuses on improvement and innovation of two technical nodes (seed disinfection and callus formation and differentiation) in culture steps. With adoption of a mercury bichloride and hydrogen peroxide compound disinfection method, the invention realizes the purposes of thorough disinfection effect and low pollution rate and minimum damage to seeds. Finally, the invention solves the problem of severe pollution to current explants. Meanwhile, a method of TDZ and 6-BA is adopted, thereby, the callus induction rate and the formation rate are remarkably increased, the callus quality is improved, the differentiation capacity of the callus is reserved to a maximum extent, the formation of embryoid sprouts is promoted so that the novel technical method of the tissue culture of the Medicago sativa L. which is efficient, saves time, and has low toxicity and low browning, is obtained.

The invention discloses a composite support containing a PGA strengthening net, a preparation method and applications thereof. The support comprises at least one layer of PGA strengthening net and a collagen/chitosan porous support, which is casted on the PGA strengthening net; the pore size of the collagen/chitosan porous support is in a range of 30 to 500 [mu]m, the diameter of the net hole of the PGA strengthening net is in a range of 200 to 3000 [mu]m, and the diameter of single PGA wire is in a range of 10 to 200 [mu]m. The composite support prepared by the provided preparation method has the advantages of good biocompatibility, excellent mechanical properties, and specific three-dimensional porous structure, can be applied to constructions of organism and tissues, thus promotes the healing and filling of damaged tissues, and improves the healing quality.

The invention provides an environment-friendly and simple preparation method for a hyaluronic acid-chitosan microsphere carrying an epidermal growth factor. The hyaluronic acid-chitosan microsphere is prepared via ionic polymerization, centrifugation, purification, and freeze-drying under the protection of a protective agent. The hyaluronic acid-chitosan microsphere carrying the epidermal growth factor can be used for preparing pharmaceutical preparations (including a gel, a film agent, a powder agent, an ointment, and the like) for treating burn and scald wounds. According to the hyaluronic acid-chitosan microsphere carrying the epidermal growth factor and the pharmaceutical preparations of the hyaluronic acid-chitosan microsphere, sustained release of the epidermal growth factor on the burn and scald wounds is realized; wound cell proliferation is promoted; and functions, of protecting burn and scald tissues and promoting wound healing, of sodium hyaluronate and chitosan are developed. The hyaluronic acid-chitosan microsphere can provide a support for wound cell proliferation, and an excellent medicine for treating burn and scald wounds is obtained.

The invention relates to a Chinese medicinal compound preparation for treating fracture, which comprises the following Chinese medicine raw materials in part by weight: 8 to 12 parts of peach seed, 13 to 17 parts of safflower, 8 to 12 parts of Szechuan lovage rhizome, 8 to 12 parts of red paeony root, 8 to 12 parts of Chinese angelica, 8 to 12 parts of root of red-rooted salvia, 13 to 17 parts of fortune drynaria rhizome, 13 to 17 parts of Himalayan teasel root and 2 to 4 parts of notoginseng root (electuary). A preparation method comprises the following steps of: cleaning the nine medicinal materials, mixing in a ratio, adding water in an amount which is 4 to 7 times the total weight of the medicinal materials or 70 volume percent ethanol, decocting for two times, 80 minutes each time, mixing two-time decoction, filtering, and concentrating to obtain liquid extract of which the specific gravity is 1.18 at the temperature of 60 DEG C. The conventional preparation process is adopted. The preparation can be prepared into any one pharmaceutical formulation such as tablets, capsules, granules, oral liquid, pills and syrup. By the Chinese medicinal compound preparation prepared by the method, fracture healing time is obviously shortened, calluses grow more quickly, firmly and reliably, and the curative effect is far superior to that of the conventional Chinese medicine for treating the fracture.

The invention discloses a simulation system for simulating a fracture healing process, which relates to the field of biochemical engineering. The simulation system is used for predicting the complex process of fracture healing and providing guidance for doctors in establishing a good surgical program for fracture healing. The system comprises a three-dimensional geometric modeling module, a finite element creation module, a fracture site biomechanical-modeling module, a fracture site cell evolution modeling module, a callus growth modeling module and a display module. Cell concentration and tissue material properties are updated by the fracture site biomechanical-modeling module and the fracture site cell evolution modeling module; the geometric shape of callus is updated by the callus growth modeling module; and the effect of fracture healing is judged by the display module. The simulation system can more accurately simulate the process of fracture healing and repetitively carry out experimental simulation for unlimited times, thus providing help for making the best surgical program for fracture healing.

A fracture healing simulation method based on cell activity relates to the field of biomedical engineering and is used for predicting a complex process of fracture healing and seeking an optimal fracture healing scheme. The method comprises the steps of I, establishing a three-dimensional geometric model of a fracture region; II, establishing a fracture healing region finite element model; III, acquiring material attribute and cell concentration of a fracture region unit; IV, subjecting the fracture region to finite element analysis; V, solving mechanical stimulus borne by the fracture region unit, and acquiring new tissue phenotype according to force regulating algorithm; VI, analyzing cell activity; VII, calculating material attribute of a new fracture healing region unit; VIII, judging whether the program ends or not. Through the fracture healing simulation method based on cell activity, it is possible to simulate a fracture healing process more precisely, and doctors are helped to make an optimal fracture healing surgical scheme.

The invention provides an oral toothpaste with anti-helicobacter pylori and anti-caries and stomach-enhancing functions. The toothpaste contains bismuth glycyrrhizinate at a concentration of 0.001% to 10% by weight. Bismuth Glycyrrhizinate combines the anti-inflammatory, analgesic, anti-ulcer, anti-oxidation effects of glycyrrhizic acid and the gastric mucosal protection function of bismuth. The bismuth glycyrrhizinate toothpaste of the present invention is a kind of daily oral hygiene article, practical and convenient, multi-effect of one product, can effectively kill HP and cariogenic bacteria, simultaneously bismuth glycyrrhizinate is dental plaque inhibitory and cleaning agent, thereby helps to inhibit and remove dental plaque, reduce the chance of bacterial adhesion and aggregation, and treat both symptoms and root causes; making it into toothpaste for external use can effectively, conveniently and durably kill Helicobacter pylori in the oral cavity, thereby effectively preventing gastrointestinal The purpose of treating tract infection and preventing the recurrence of gastritis, gastric and duodenal ulcers.

The invention discloses a drug preparation for treating chronic diabetic ulcers, which consists of hydroxysafflor yellow A, deferoxamine mesylate and pharmaceutical excipient. In the drug preparation,the percentage by weight of the hydroxysafflor yellow A is 0.02 to 0.2 percent, and the percentage by weight of deferoxamine mesylate is 0.02 to 0.2 percent. The invention further discloses a preparation method of the drug preparation. On the basis of keeping the original pharmacological activity of the traditional Chinese medicine extract hydroxysafflor yellow A, the chemical drug, i.e. low-molecular weight deferoxamine mesylate, is jointly used by the drug preparation to enhance the neovascularization effect of the hydroxysafflor yellow A, realizing the strategy of anti-inflammation and vascular remodeling multi-target therapy and increasing the therapeutic effect of drug combination.

The invention discloses a stomach medicine which is characterized by comprising the following Chinese medical herbs in parts by weight: 80-120 parts of rubber, 120-180 parts of dragon's blood, 80-120 parts of ruddle, 150-250 parts of calcined ark shell and 120-180 parts of Fritillaria hupehenis Hsiao et K.C.Hsia. The traditional formula is integrated and improved and the Chinese medicinal herbs are matched with mineral medicines, thus the stomach medicine has the advantages of special formula, accurate treatment effect, low cost, no toxic and side effects, and smooth medicine properties, and has the effects of soothing liver and harmonizing stomach, promoting qi circulation and relieving pain, relieving the stasis and refreshing mind, regulating the flow of vital energy and removing obstruction toit, reducing phlegm, promoting qi circulation and removing obstruction in the collateral, dredging the Shao-Yang channels, warming without drying, and tonifying without causing stagnation; and the stomach medicine has ulcer cure rate of 93 percent in 8 weeks for the peptic ulcer and disappear rate of 95 percent for the chronic gastritis, remarkable treatment effect on the chronic atrophic gastritis which can not be effectively treated at present, and no toxic and side effects through chute and long term toxicity tests.

The invention provides a simulated tendinous sheath film which adopts a double-layered structure. The simulated tendinous sheath film comprises a fibrous layer and a synovial layer, wherein the fibrous layer is made of degradable high polymer material, and does not contain hyaluronic acid (HA) or metal salt; and the synovial layer is made of degradable high polymer material, and contains hyaluronic acid (HA) or metal salt. The synovial layer contains the hyaluronic acid (HA) or metal salt, thereby realizing the controlled release of HA or the metal salt, simulating the function of secreting HA by the tendinous sheath inner layer, providing a long-acting lubrication action environment for tendon, promoting the endogenous healing of the tendon, improving the tendon healing quality, and improving the postoperation function of the tendon; and the fibrous layer mainly serves as a physical barrier, thereby reducing the paratenon adhesion, and preventing exogenous healing. Moreover, the biodegradable high polymer material can be automatically degraded, is non-toxic, free from immunogenicity, and good in biocompatibility, improves the curative effect, and reduces the side effect.

A Chinese medicine for setting up bone and treating closed bone fracture is prepared from 11 Chinese-medicinal materials including Chinese angelica root, ground beetle, borneol, notoginseng, etc.

The invention discloses a skin anastomat. The skin anastomat comprises an elastic thread, a first sheath pipe, a tensioning assembly, a second sheath pipe, a plaster, a bolt and a zipper, wherein thefirst sheath pipe penetrates through the tensioning assembly to be arranged on the plaster; the zipper is arranged at one end of the first sheath pipe; the second sheath pipe is arranged at the otherend of the first sheath pipe; the bolt is rotatably arranged in the second sheath pipe; and the elastic thread is arranged in the first sheath pipe, and is connected with the zipper and the bolt; andthe tensioning assembly can extend and retract the elastic thread. According to the skin anastomat disclosed by the invention, the pinching distance and the pinching pulling force of skin on two sidesof a wound are adjustable, and the healing quality of the wound is increased.

The invention discloses a diabetic foot ulcers (DFC) external medicine, and relates to the technical field of medical preparations containing traditional Chinese medicinal herbs. The DFC external medicine is medicinal liquor prepared from the following traditional Chinese medicine compositions in parts by weight: 2-5 parts of unprocessed semen strychni, 2-5 parts of unprocessed radix aconiti sinensis, 2-5 parts of unprocessed radix aconiti agrestis, 3-6 parts of radix et rhizoma rhei, 4-6 parts of dahurian loosestrife herb with root, 4-6 parts of folium ilicis chinensis, 180-230 parts of rhizoma zingiberis recens and 180-230 parts of mung bean; the medicinal liquor is prepared by brewing 1900-2300 parts of baijiu with a degree of 50-65. The DFC external medicine disclosed by the inventionhas the efficacy of relieving swelling and pain, disinfecting, activating blood, dissipating blood stasis, eliminating putridity and engendering flesh, and has a remarkable effect on curing DFC; compared with a plaster preparation and a powder preparation, a liquor preparation is convenient to administer; a clean operation during medicine change is convenient and rapid; the medicine is simple in preparation; the used traditional Chinese medicines are common medicines, have no need to be prepared; there is no high requirement for manufacturing technologies, and patients can make by themselves;there is little toxic or side effect.

The invention provides a decellularized flap as well as a preparation method and application thereof, and relates to the technical field of clinical medicine, biomedicine and regenerative medicine. The preparation method of the decellularized flap, which is provided by the invention, comprises the following steps of infusing a heparin solution, a Triton X-100 solution and an SDS ((Sodium Dodecyl Sulfate) solution in sequence, and then carrying out drying, sterilization and storage treatments. Operation steps are simple; the preparation is short in consumed time. The decellularized flap provided by the invention has no an immunological rejection reaction, does not relate to hypertoxic and harmful chemical substances, and further has favorable cytocompatibility and histocompatibility. Through the application of the decellularized flap provided by the invention to the repair of the wound surface of a defect and to scientific research, the restoration of defects of a full-thickness skin and a subcutaneous tissue can be realized; the healing quality is good.