8 results about "Tissue engineered skin" patented technology

The invention belongs to the field of biomedicine, and particularly relates to a preparation method of a full-biological humanized bionic dermal tissue. The invention provides a construction method of'cell sheet-scaffold material adaptation 'by combining a cell sheet technology and a macroporous hydrogel technology. The preparation method comprises the following steps: preparing an NHDF cell patch, compounding the NHDF cell patch and macroporous hydrogel, co-culturing, and guiding cells to migrate and proliferate into the hydrogel by taking HAMA macroporous hydrogel as a sacrifice scaffold, so as to construct a millimeter-level human-derived bionic dermal tissue. And it is proved that the human-derived bionic dermal tissue with high activity and high mechanical property can be efficiently constructed through the strategy of combining the macroporous hydrogel and the cell patch. The bionic dermal tissue prepared by the method has better mechanical properties and biological functions. The achievement provides an important technical route and theoretical basis for developing tissue engineering skin products with clinical applicability.

This invention discloses a method for constructing tissue-engineered skin organoids. After dissecting primary skin organoids induced and differentiated from iPSCs, transparent sac-like structures are seeded onto a decellularized porcine skin scaffold with the epidermis facing upwards and the dermis facing downwards. The tissue-engineered skin organoids are then cultured using an air-liquid interface culture system to construct a structure closely resembling the physiological and anatomical structure of human skin. The tissue-engineered skin organoids provided by this invention can be applied to disease model construction, drug screening and toxicity testing, skin injury and repair regeneration, and skin development. UVB irradiation of the tissue-engineered skin organoids can induce aging in human skin organoids, thus constructing a UVB-induced skin organoid aging model.

The invention belongs to the technical field of tissue engineering skin and drug testing, and particularly relates to a regenerated skin drug testing method which comprises the following steps: firstly, collecting in-vitro human skin samples of different genders, ages and anatomical areas to establish a sample library, then selecting the samples according to test requirements, and dividing the samples into test samples and culture samples; a culture sample is used for 3D printing of regenerated skin, and through comparison of IVRT / IVPT test data of a test sample and a regenerated skin sample and multi-round optimization of a printing strategy, regenerated skin with the skin type close to that of in-vitro skin is finally manufactured in batches and used for tests of multiple drug application strategies. By optimizing the 3D printing strategy, the 3D printing regenerated skin manufactured in batches can reflect gender, age and anatomical area information more accurately, the use amount requirements of various drug application strategies are met, test errors are reduced, and challenges caused by the large use amount of the in-vitro skin are reduced.

The invention discloses in-vitro tissue engineering skin automatic cleaning equipment, and belongs to the technical field of cleaning equipment, the in-vitro tissue engineering skin automatic cleaning equipment comprises a cleaning tank, an ultrasonic cleaning module is arranged in the cleaning tank, the rear side of the cleaning tank is communicated with a liquid discharge pipe, a partition plate is mounted in an inner cavity of the cleaning tank, and the ultrasonic cleaning module is located at the top of the partition plate. According to the tissue engineering skin cleaning and rubbing device, the cleaning and rubbing effect on tissue engineering skin is improved through mutual circumferential movement of the pretreatment rollers on the two sides, the pretreated tissue skin falling into the surface of the conveying roller belt can be conveyed to the compaction mechanism on the rear side, and ultrasonic cleaning is conducted through ultrasonic waves emitted by the ultrasonic cleaning module in the cleaning tank in the compaction process; according to the in-vitro tissue engineering skin cleaning device, residual impurities of tissue skin are cleaned into a medium in the cleaning tank through ultrasonic waves, automatic cleaning of the in-vitro tissue engineering skin is achieved through pretreatment and compaction superposition cleaning, and cleaned materials can be conveyed to the tail section of the cleaning tank through a transmission roller to be conveniently taken and used.

The invention belongs to the technical field of biological materials. The preparation steps of the existing composite injectable gel are tedious, and the prepared composite injectable gel has the problems of poor injectability, low swelling rate, low mechanical strength, low drug loading rate, high cytotoxicity and the like. On the basis, the invention provides a method for preparing the composite injectable gel, and the method comprises the following steps: 1) dissolving hyaluronic acid and sodium chloride in an aqueous solution of NaOH or KOH; (2) adding a cross-linking agent 1, 4-butanediol diglycidyl ether or genipin, and mixing; 3) carrying out a cross-linking reaction; 4) purifying a reaction product; and 5) performing vacuum freeze-drying to obtain the final composite injectable gel. According to the method, the preparation process is simplified, and the prepared composite injectable gel has significantly improved injectability, swelling ratio, mechanical strength and drug loading rate, and lower cytotoxicity, and has good application prospects in the fields of drug delivery, tissue engineering, skin wound healing and repair, medical cosmetology and the like.

The invention discloses tissue engineering skin with a skin appendage reconstruction function and a preparation method and application thereof. The tissue engineering skin with the skin appendage reconstruction function is obtained by taking a decellularized pigskin matrix containing a basement membrane band as a scaffold material and planting a hiPSCs-sourced skin-imitating cell component seed on the scaffold material and then culturing the hiPSCs-sourced skin-imitating cell component seed. The stent material is a decellularized pigskin matrix containing a basement membrane band, and the basement membrane band is of a structure lacked by stent materials in the market at present. However, the basilar membrane has the functions of supporting and connecting the epidermal layer and the corium layer, signal transduction and permeation barrier, so that the basilar membrane is very important in tissue engineering skin. Meanwhile, the decellularized pigskin scaffold is good in biocompatibility, high in biological activity and good in strength and toughness, and materials are easy to obtain.

The invention relates to the technical field of material preparation, in particular to composite bio-ink for constructing tissue engineering skin through 3D printing and a preparation method and application of the composite bio-ink. The oxidized sodium alginate-gelatin bio-ink forms a Schiff base network through oxidized sodium alginate and gelatin to complete gelation. The preparation method comprises the following steps: performing in-situ reduction on a # imgabs0 # solution through polydopamine to obtain an AgNPs colloidal solution; the composite bio-ink provided by the invention has good printability and mechanical properties, and can be used for 3D bio-printing; the prepared scaffold has excellent biocompatibility, also has the functions of self-healing, resisting bacteria and promoting proliferation and differentiation of skin fibroblasts, can be used for preparing tissue engineering skin scaffolds and promoting wound healing and functional reconstruction of skin tissues, and has a relatively high application prospect.

The present application belongs to the technical field of biomaterials. The existing preparation process of composite injectable gel is complicated, and the prepared composite injectable gel has the problems of poor injectability, low swelling rate, low mechanical strength, low drug loading rate and high cytotoxicity. Based on this, the present application provides a method for preparing a composite injectable gel, which comprises: 1) dissolving hyaluronic acid and sodium chloride in an aqueous solution of NaOH or KOH; 2) adding a crosslinking agent 1,4-butanediol diglycidyl ether or genipin and mixing; 3) performing crosslinking reaction; 4) purifying the reaction product; 5) vacuum freeze-drying to obtain the final composite injectable gel. The method simplifies the preparation process, and the prepared composite injectable gel has significantly improved injectability, swelling rate, mechanical strength, drug loading rate, and lower cytotoxicity, and has good application prospect in the fields of drug delivery, tissue engineering, skin wound healing repair, medical cosmetology and the like.