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Organization engineering skin containing adipose layer and method of preparing the same

A tissue engineering skin and fat layer technology, applied in medical science, prostheses, etc., can solve the problems of tissue engineering skin products without fat layer, improve healing quality, reduce scar hyperplasia, and reduce shrinkage Effect

Inactive Publication Date: 2008-03-12
陕西艾尔肤组织工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Organization engineering skin containing adipose layer and method of preparing the same

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Embodiment 1

[0026] Step 1. Preparation of culture medium: F12 commercial culture medium and fetal bovine serum were mixed as base solution according to the volume ratio of 9:1; then 2 ng of insulin, 10 μg of hydrocortisone, and 2 μg of epidermal growth factor were added according to the standard of 500 ml of base solution. Basic fibroblast growth factor 2ng, adenine 12mg, transferrin 5mg, and vitamin C 5mg were prepared as basal medium; then the basal medium was used to prepare the following medium, and the concentration of the additives was the final concentration.

[0027]Medium a: basal medium + bovine pituitary extract 25μg / ml

[0028] Medium b: basal medium + calcium chloride 0.1mM

[0029] Medium c: basal medium + calcium chloride 0.2mM

[0030] Medium d: basal medium + calcium chloride 0.3mM

[0031] Step 2. Preparation of bio-scaffold material: at 4°C, mix collagen 7: chitosan 0.5: hyaluronic acid 3: chondroitin sulfate 0.5 by mass ratio, and use 0.5M acetic acid to prepare a co...

Embodiment 2

[0037] Step 1. Preparation of culture medium: F12 commercial culture medium and fetal bovine serum were mixed as base solution according to the volume ratio of 9:1; then 50 ng of insulin, 500 μg of hydrocortisone, and 10 μg of epidermal growth factor were added according to the standard of 500 ml of base solution. 40ng of basic fibroblast growth factor, 17mg of adenine, 10mg of transferrin, and 30mg of vitamin C were prepared into a basal medium; the following medium was prepared with the basal medium, and the concentration of the additives was the final concentration:

[0038] Medium a: basal medium + bovine pituitary extract 25μg / ml

[0039] Medium b: basal medium + calcium chloride 1mM

[0040] Medium c: basal medium + calcium chloride 2mM

[0041] Medium d: basal medium + calcium chloride 3mM

[0042] Step 2. Bio-scaffold material preparation: under the condition of 4°C, collagen 10: chitosan 1: hyaluronic acid 2: chondroitin sulfate 1 was mixed by mass ratio, and it was...

Embodiment 3

[0048] Step 1. Preparation of culture medium: F12 commercial culture medium and fetal bovine serum were mixed as base solution according to the volume ratio of 9:1; then 40 ng of insulin, 100 μg of hydrocortisone, and 10 μg of epidermal growth factor were added according to the standard of 500 ml of base solution. Basic fibroblast growth factor 15ng, adenine 15mg, transferrin 6mg, and vitamin C 30mg were prepared into a basal medium; then the basal medium was used to prepare the following medium, and the concentration of the additives was the final concentration:

[0049] Medium a: basal medium + bovine pituitary extract 25μg / ml

[0050] Medium b: basal medium + calcium chloride 0.5mM

[0051] Medium c: basal medium + calcium chloride 1mM

[0052] Medium d: basal medium + calcium chloride 2mM

[0053] Step 2. Under the condition of 4 DEG C, collagen 9: chitosan 1: hyaluronic acid 2.5: chondroitin sulfate 1 is mixed by mass ratio, and it is prepared into a concentration of 10...

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Abstract

The present invention is a tissue engineering skin containing the fat layer and the preparation method. The present invention is characterized in that the present invention is manufactured by the fat cell, the skin fibroblast cell, the epidermal cell and the biological frame. The fat cell and the skin fibroblast cell are mixed inside the biological frame. The active tissue engineering skin containing the fat layer is built by planting the epidermal cell on the surface. The prepared issue engineering skin containing the fat layer not only has a certain degree of elastic and toughness properties, but also has short incubation time and no significant immunological rejection. The tissue has similar structure as the natural skin and the subcutaneous adipose tissue. The present invention has multiple effects in aspects of anti infection, homeostasis and inhabiting the scar formation.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering of biological materials, and in particular relates to a tissue-engineered skin containing fat layer and a preparation method thereof. Background technique [0002] In recent years, in the fields of medical cosmetology, trauma, burn surgery, etc., with the requirements for functional recovery and aesthetic considerations, it is far from enough to achieve skinization on the wound surface for some deep skin defects, and efforts need to be made to explore applications A more reasonable method, so that the skin graft area can finally achieve the same or similar results as skin flap transplantation. [0003] Studies have shown that the cytokines secreted by adipocytes in adipose tissue can promote the growth and crawling of epidermal cells at the wound edge, the formation of dermo-epidermal junctions, improve the mechanical properties of grafts, and have a cosmetic effect on filling soft tiss...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/38A61L27/18
Inventor 金岩张勇杰陆伟
Owner 陕西艾尔肤组织工程有限公司
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