Tissue engineering skin containing blood vessel structure and its construction method

A tissue-engineered skin and vascular structure technology, applied in skin transplantation, medical science, prosthesis, etc., can solve problems such as defective product cost in mature development cycle and imperfect tissue engineering products.

Inactive Publication Date: 2004-05-05
陕西艾尔肤组织工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Although tissue engineering methods have made great progress in the development of skin, and some products have been applied clinically, the tissue engineering pr

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  • Tissue engineering skin containing blood vessel structure and its construction method
  • Tissue engineering skin containing blood vessel structure and its construction method
  • Tissue engineering skin containing blood vessel structure and its construction method

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Embodiment Construction

[0027] The method of the present invention and implementation effect will be further described in conjunction with experiment now.

[0028] 1. Culture of vascular endothelial cells

[0029] Take the umbilical cord of the newborn, put it in sterile phosphate buffered saline PBS, store and transport it at 4°C, try to start the culture within 1 hour, otherwise the cell viability will be greatly reduced; put the umbilical cord in a sterile petri dish and buffer it with sterile PBS The umbilical cord vein was tightly ligated with a suture thread, and the digestive solution a was poured into it for 10 minutes at 37°C for 10 minutes, and then digested with the digestive solution b for 5 minutes; Cut the ligated umbilical cord from one end, pour the liquid out, then repeatedly wash the inner cavity of the blood vessel with PBS, collect the liquid, centrifuge, and wash twice; resuspend the cells with vascular endothelial cell culture medium, and inoculate and culture. The digestive so...

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Abstract

A tissue-engineered skin containing vascular structure features that it contains skin fibroblasts, keratinous cells and vascular endothelial cells. Its configuring method includes separating said three kinds of cells from eath other, external culture, proportionally mixing, and configuring the artificial skin with vascular structure. Its advantages are high survival rate and high resistance to infection.

Description

technical field [0001] The invention belongs to the technical field of constructing artificial organs by tissue engineering method in biomedical engineering, and relates to a tissue engineering skin containing blood vessel structure and a construction method thereof. Background technique [0002] In recent years, with the development of tissue engineering technology, there have been more and more reports on the research and development of biological substitutes that can repair, maintain or improve the function of damaged tissues, and there are also many research reports on artificial skin substitutes. At present, the research and development of tissue engineered skin has become a hot spot in skin defect transplantation. [0003] The skin is an important tissue and organ that covers and protects the body surface. Defects and abnormalities of the skin are often caused by inflammation, ulcers, trauma, burns, postoperative tumors, and congenital deformities. For this kind of ti...

Claims

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Application Information

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IPC IPC(8): A61F2/10A61L27/00A61L27/60
Inventor 金岩刘源赵宇
Owner 陕西艾尔肤组织工程有限公司
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