Preparation method of tissue engineering skin containing hair follicles

A tissue-engineered skin and tissue-engineering technology, applied in the field of tissue-engineered skin preparation, can solve problems such as unsatisfactory

Inactive Publication Date: 2010-07-14
FOURTH MILITARY MEDICAL UNIVERSITY +1
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  • Application Information

AI Technical Summary

Problems solved by technology

Simulating the conditions for the formation of hair follicles in the early embryo, mixing epithelial cells and mesenchymal cells can achieve hair follicle regeneration through in vivo culture; most of them use hair follicle regeneration-inducing hair papilla cells and hair follicle out

Method used

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Embodiment Construction

[0026] The specific implementation of the technical solution of the present invention will be described in detail below in conjunction with examples.

[0027] Step 1. Prepare culture medium

[0028] Culture medium A: In 500ml of commercial DMEM medium containing V / V of 15% fetal bovine serum, 6 μg of epidermal growth factor EGF, 10 ng of fibroblast growth factor bFGF, and 25 μg / ml of bovine pituitary extract BPE were added;

[0029] Culture medium B: Add EGF 6 μg, bFGF 10 ng, BPE 25 μg / ml, insulin-like growth factor IGF-110 ng in 500 ml of serum-free commercial Williams E culture medium;

[0030] Culture medium C: In 500ml of commercial DMEM medium containing 10% fetal bovine serum at V / V, add 10ng of insulin, 100μg of hydrocortisone, 15mg of adenine, 10mg of Vc, 6μg of EGF, 10ng of bFGF, and 25μg of BPE / ml;

[0031] Culture solution D: Calcium chloride containing 0.8mM concentration in culture solution C;

[0032] Culture solution E: Calcium chloride at a concentration of...

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Abstract

The invention relates to a preparation method of tissue engineering skin containing hair follicles. In the invention, tissue engineering hair follicles are evenly inoculated in a tissue engineering dermal layer formed by compounding a bracket material and cells, then the tissue engineering dermal layer is covered by epidermal cells, and the tissue engineering skin containing the hair follicles is formed after culture; the invention overcomes the defects of little number of the hair follicles in the skin, no uniformity and uncontrollable quantity of the hair follicles caused by mixed injection formerly, and the prepared tissue engineering skin contains an epidermis, dermis and hair follicle type tissue structure, wherein the number of the hair follicles approaches to the natural number, the hair follicles are evenly distributed in artificial pores and develop continuously after in-vitro culture and transplantation, a multi-layer concentric circle structure is formed, an inner layer is hair papilla cells wrapt by micro-capsule membranes, and an outer layer is epithelium class cells; and the skin cultured in vitro or transplanted in vivo finally forms functional skin containing a hair follicle accessory structure, and the regeneration of the hair follicles after skin transplantation can be realized.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering of biological materials, and in particular relates to a preparation method of tissue engineering skin containing hair follicles. Background technique [0002] Tissue engineering is a research hotspot in the field of life sciences, and related research involves various aspects. Its content is to plant functional cells cultured in vitro on corresponding scaffold materials, and form tissues or organs with certain functions after culture. With the rapid development of tissue engineering technology, tissue engineered skin has been widely used clinically. The current tissue-engineered skin is mainly to plant skin dermal cells and epidermal cells on the skin scaffold material. The tissue-engineered skin thus constructed has a two-layer structure of dermis and epidermis similar to normal skin, but it does not have hair follicles, sebaceous glands and other skin accessory organs. , There are s...

Claims

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Application Information

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IPC IPC(8): C12N5/077C12N5/071A61L27/60
CPCC12N2502/094C12N5/0698C12N2502/1323A61L27/38A61L2430/18A61L2400/06
Inventor 金岩凡孝菊张勇杰王爱军
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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