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Preparation method of double-layer tissue engineering skin comprising basement membrane structure

A technology of tissue engineering skin and double-layer tissue, which is applied in the field of basement membrane formation between the dermis and epidermis, can solve the problems of high research standards and inapplicability, and achieve the effect of improving performance and maintaining integrity

Active Publication Date: 2007-03-07
威力保生物技术(成都)有限公司
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  • Abstract
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Problems solved by technology

However, the research standard of tissue engineered skin is high, the ingredients are clear, non-toxic and harmless, so this method is not suitable for the construction of basement membrane for tissue engineered skin
After searching, no technical literature has been published about the preparation method of double-layer tissue-engineered skin with basement membrane structure.

Method used

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  • Preparation method of double-layer tissue engineering skin comprising basement membrane structure
  • Preparation method of double-layer tissue engineering skin comprising basement membrane structure
  • Preparation method of double-layer tissue engineering skin comprising basement membrane structure

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Embodiment Construction

[0025] The following is a preparation example of tissue-engineered skin containing basement membrane structure compound chitin, but the method of the present invention is by no means limited to the preparation example given.

[0026] First, add a calcium chloride solution with a concentration of 6M to the DMEM medium, adjust the calcium ion concentration to 1.85mM, then add a laminin solution with a concentration of 200μg / ml, adjust the concentration of laminin to 20μg / ml, and prepare the obtained tissue Engineering skin culture medium; mix collagen acetate, chitin, hyaluronic acid and elastin in a weight ratio of 16:2:1:1, adjust the pH value of the gel solution to 7.2 with 1N sodium hydroxide, and prepare gel Gel solution 20ml; add fibroblasts 1×10 to the gel solution 6 , mix well; inhale into the culture dish, after 15 minutes, gently add keratinocyte suspension 1×10 6 , and finally add 8ml of the prepared tissue engineering skin culture medium, place at 37°C, 5% CO 2 Cul...

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Abstract

The invention relates to a method for producing dual-layer organism skin with basilar membrane. Wherein, it is formed by preparing organism skin cultivate base, preparing gel solution, cultivating breeding box and cultivating screen support; it adjusts the calcium density of cultivate base and adds external laminin to accelerate the basilar membrane formation, to strengthen the connection between organism protective skin and corium. The invention has simple process while all materials are liquid and better compatible, without hurting transplanted one; and the skin will not be separated easily; and the product has better compatibility.

Description

1. Technical field [0001] The invention belongs to tissue engineering, and specifically relates to a method for promoting the formation of a basement membrane between the epidermis of double-layer tissue engineering skin by adding exogenous laminin. 2. Background technology [0002] The basement membrane is an important structure with a thickness of about 0.5-1.0 μm located between the epidermis and the dermis. It is mainly composed of macromolecules such as type IV collagen, type VII collagen, nestin, and laminin. PAS staining shows a purple-red homogeneous band . The basement membrane has many important biological functions. The most obvious function is to tightly connect the epidermis and dermis, provide a strong combination for the epidermis and dermis, and avoid epidermal separation under physiological conditions; another significant function is to determine the polarity of the epidermis and provide epidermal migration. In addition, it plays an irreplaceable role in ma...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/60
Inventor 伍津津鲁元刚雷霞
Owner 威力保生物技术(成都)有限公司
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